Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_C04
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 132 1e-31
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 130 6e-31
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 130 6e-31
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 129 1e-30
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 129 1e-30
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 129 1e-30
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 116 2e-26
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 111 4e-25
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 111 4e-25
At4g20920.1 68417.m03032 double-stranded RNA-binding domain (DsR... 28 4.7
At3g63380.1 68416.m07135 calcium-transporting ATPase, plasma mem... 28 6.2
At3g21180.1 68416.m02677 calcium-transporting ATPase, plasma mem... 27 8.2
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 132 bits (320), Expect = 1e-31
Identities = 61/102 (59%), Positives = 78/102 (76%)
Frame = +3
Query: 348 AQNYTFQTEVNRMMKLIINSLYRNKEIXLRELISNGXDALDKIRLMSLTDRGVLEANPEL 527
A+ + FQ E+N+++ LIIN+ Y NKEI LRELISN DALDKIR SLTD+ L+ PEL
Sbjct: 9 AETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQPEL 68
Query: 528 XIRIKAEPDKRLLHIIDSGVGMTRADLINNLGTIAKSGTADF 653
IR+ + + L IIDSG+GMT+ADL+NNLGTIA+SGT +F
Sbjct: 69 FIRLVPDKSNKTLSIIDSGIGMTKADLVNNLGTIARSGTKEF 110
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 130 bits (315), Expect = 6e-31
Identities = 72/136 (52%), Positives = 93/136 (68%), Gaps = 2/136 (1%)
Frame = +3
Query: 252 GSRTDAEAVLREEEAISPDALSVAQMKELRDRAQNYTFQTEVNRMMKLIINSLYRNKEIX 431
G TD++ V RE E++S K LR A+ + FQ EV+R+M +IINSLY NK+I
Sbjct: 52 GLSTDSDVVHRESESMSK--------KTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIF 103
Query: 432 LRELISNGXDALDKIRLMSLTDRGVLEA--NPELXIRIKAEPDKRLLHIIDSGVGMTRAD 605
LRELISN DALDKIR ++LTD+ VL +L I+IK + K++L I D G+GMT+ D
Sbjct: 104 LRELISNASDALDKIRFLALTDKDVLGEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKED 163
Query: 606 LINNLGTIAKSGTADF 653
LI NLGTIAKSGT+ F
Sbjct: 164 LIKNLGTIAKSGTSAF 179
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 130 bits (315), Expect = 6e-31
Identities = 72/136 (52%), Positives = 93/136 (68%), Gaps = 2/136 (1%)
Frame = +3
Query: 252 GSRTDAEAVLREEEAISPDALSVAQMKELRDRAQNYTFQTEVNRMMKLIINSLYRNKEIX 431
G TD++ V RE E++S K LR A+ + FQ EV+R+M +IINSLY NK+I
Sbjct: 52 GLSTDSDVVHRESESMSK--------KTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIF 103
Query: 432 LRELISNGXDALDKIRLMSLTDRGVLEA--NPELXIRIKAEPDKRLLHIIDSGVGMTRAD 605
LRELISN DALDKIR ++LTD+ VL +L I+IK + K++L I D G+GMT+ D
Sbjct: 104 LRELISNASDALDKIRFLALTDKDVLGEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKED 163
Query: 606 LINNLGTIAKSGTADF 653
LI NLGTIAKSGT+ F
Sbjct: 164 LIKNLGTIAKSGTSAF 179
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 129 bits (312), Expect = 1e-30
Identities = 61/102 (59%), Positives = 76/102 (74%)
Frame = +3
Query: 348 AQNYTFQTEVNRMMKLIINSLYRNKEIXLRELISNGXDALDKIRLMSLTDRGVLEANPEL 527
A+ + FQ E+N+++ LIIN+ Y NKEI LRELISN DALDKIR SLTD+ L+ PEL
Sbjct: 4 AETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQPEL 63
Query: 528 XIRIKAEPDKRLLHIIDSGVGMTRADLINNLGTIAKSGTADF 653
I I + L IIDSG+GMT+ADL+NNLGTIA+SGT +F
Sbjct: 64 FIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEF 105
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 129 bits (312), Expect = 1e-30
Identities = 61/102 (59%), Positives = 76/102 (74%)
Frame = +3
Query: 348 AQNYTFQTEVNRMMKLIINSLYRNKEIXLRELISNGXDALDKIRLMSLTDRGVLEANPEL 527
A+ + FQ E+N+++ LIIN+ Y NKEI LRELISN DALDKIR SLTD+ L+ PEL
Sbjct: 4 AETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQPEL 63
Query: 528 XIRIKAEPDKRLLHIIDSGVGMTRADLINNLGTIAKSGTADF 653
I I + L IIDSG+GMT+ADL+NNLGTIA+SGT +F
Sbjct: 64 FIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEF 105
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 129 bits (312), Expect = 1e-30
Identities = 61/102 (59%), Positives = 76/102 (74%)
Frame = +3
Query: 348 AQNYTFQTEVNRMMKLIINSLYRNKEIXLRELISNGXDALDKIRLMSLTDRGVLEANPEL 527
A+ + FQ E+N+++ LIIN+ Y NKEI LRELISN DALDKIR SLTD+ L+ PEL
Sbjct: 4 AETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQPEL 63
Query: 528 XIRIKAEPDKRLLHIIDSGVGMTRADLINNLGTIAKSGTADF 653
I I + L IIDSG+GMT+ADL+NNLGTIA+SGT +F
Sbjct: 64 FIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEF 105
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 116 bits (278), Expect = 2e-26
Identities = 54/102 (52%), Positives = 78/102 (76%)
Frame = +3
Query: 348 AQNYTFQTEVNRMMKLIINSLYRNKEIXLRELISNGXDALDKIRLMSLTDRGVLEANPEL 527
A+ + +Q EV+R+M LI+NSLY NKE+ LRELISN DALDK+R +S+T+ + + P+L
Sbjct: 94 AEKFEYQAEVSRLMDLIVNSLYSNKEVFLRELISNASDALDKLRYLSVTNPELSKDAPDL 153
Query: 528 XIRIKAEPDKRLLHIIDSGVGMTRADLINNLGTIAKSGTADF 653
IRI A+ + ++ + DSG+GMTR +L++ LGTIA+SGTA F
Sbjct: 154 DIRIYADKENGIITLTDSGIGMTRQELVDCLGTIAQSGTAKF 195
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 111 bits (267), Expect = 4e-25
Identities = 53/120 (44%), Positives = 81/120 (67%)
Frame = +3
Query: 294 AISPDALSVAQMKELRDRAQNYTFQTEVNRMMKLIINSLYRNKEIXLRELISNGXDALDK 473
A+ DA + + + +Q EV+R++ LI++SLY +KE+ LREL+SN DALDK
Sbjct: 58 AVKCDAAVAEKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSHKEVFLRELVSNASDALDK 117
Query: 474 IRLMSLTDRGVLEANPELXIRIKAEPDKRLLHIIDSGVGMTRADLINNLGTIAKSGTADF 653
+R +S+T+ +L +L IRIK +PD + I D+G+GMT+ +LI+ LGTIA+SGT+ F
Sbjct: 118 LRFLSVTEPSLLGDGGDLEIRIKPDPDNGTITITDTGIGMTKEELIDCLGTIAQSGTSKF 177
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 111 bits (267), Expect = 4e-25
Identities = 53/120 (44%), Positives = 81/120 (67%)
Frame = +3
Query: 294 AISPDALSVAQMKELRDRAQNYTFQTEVNRMMKLIINSLYRNKEIXLRELISNGXDALDK 473
A+ DA + + + +Q EV+R++ LI++SLY +KE+ LREL+SN DALDK
Sbjct: 58 AVKCDAAVAEKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSHKEVFLRELVSNASDALDK 117
Query: 474 IRLMSLTDRGVLEANPELXIRIKAEPDKRLLHIIDSGVGMTRADLINNLGTIAKSGTADF 653
+R +S+T+ +L +L IRIK +PD + I D+G+GMT+ +LI+ LGTIA+SGT+ F
Sbjct: 118 LRFLSVTEPSLLGDGGDLEIRIKPDPDNGTITITDTGIGMTKEELIDCLGTIAQSGTSKF 177
>At4g20920.1 68417.m03032 double-stranded RNA-binding domain
(DsRBD)-containing protein contains Pfam profile
PF00035: Double-stranded RNA binding motif
Length = 870
Score = 28.3 bits (60), Expect = 4.7
Identities = 19/50 (38%), Positives = 28/50 (56%)
Frame = -3
Query: 172 ESSRTPSPKSQICFIAGVKNIQRDLDVNYSILNNCRKC*LTQVPSFNVLS 23
E +T +PK I GVK I R +V+ S + +C L Q+P F+V+S
Sbjct: 4 EEKQTLTPKEMILQKFGVKAIYRIEEVHVSSNDCLYRCHL-QLPEFSVVS 52
>At3g63380.1 68416.m07135 calcium-transporting ATPase, plasma
membrane-type, putative / Ca(2+)-ATPase, putative
(ACA12) identical to SP|Q9LY77 Potential
calcium-transporting ATPase 12, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 12) {Arabidopsis
thaliana}; similar to SP|Q9LF79 Calcium-transporting
ATPase 8, plasma membrane-type (EC 3.6.3.8)
(Ca2+-ATPase, isoform 8) {Arabidopsis thaliana};
contains InterPro Accession IPR006069: Cation
transporting ATPase
Length = 1033
Score = 27.9 bits (59), Expect = 6.2
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = -2
Query: 344 VAQLLHLRHAQRVRGDGLLLAQHGLGVSAGSFSG*TQVGINCDLFHCANPFL 189
V ++ L+ ++ DGL L H L V S +G + + ++ H NPFL
Sbjct: 248 VGDVVFLKIGDQIPADGLFLEGHSLQVDESSMTGESD---HLEVDHKDNPFL 296
>At3g21180.1 68416.m02677 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA9)
identical to SP|Q9LU41 Potential calcium-transporting
ATPase 9, plasma membrane-type (EC 3.6.3.8)
(Ca(2+)-ATPase isoform 9) {Arabidopsis thaliana}
Length = 1086
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/38 (28%), Positives = 22/38 (57%)
Frame = -2
Query: 344 VAQLLHLRHAQRVRGDGLLLAQHGLGVSAGSFSG*TQV 231
V ++ LR +V DG+L++ H L + S +G +++
Sbjct: 290 VGDVIPLRIGDQVPADGVLISGHSLAIDESSMTGESKI 327
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,215,582
Number of Sequences: 28952
Number of extensions: 172274
Number of successful extensions: 509
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 505
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 507
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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