BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_B21
(649 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit L3... 31 0.19
SPCC306.07c |||U3 snoRNP-associated protein Cic1/Utp30 family|Sc... 26 5.4
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 25 7.1
SPAC12G12.05c |taf9||transcription initiation factor Taf9|Schizo... 25 7.1
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 25 7.1
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 25 7.1
SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|... 25 7.1
SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b |Sc... 25 9.4
>SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit
L32|Schizosaccharomyces pombe|chr 2|||Manual
Length = 103
Score = 30.7 bits (66), Expect = 0.19
Identities = 21/69 (30%), Positives = 31/69 (44%)
Frame = +2
Query: 344 IGDGFLLAVPKFRRTVEKRLNRKFGWPEYVWKPFVPKTNLKTCHECGHHHEQGRLCGNCY 523
I + LLAVPK + + K+ +R K KT + C CG LC +C+
Sbjct: 41 IWNSILLAVPKKKTSYTKKRSRLLSG-----KALKDKT-VNRCPICGSFKLAHHLCSHCF 94
Query: 524 KKIGKETKE 550
+ I +E E
Sbjct: 95 RNIRREFNE 103
>SPCC306.07c |||U3 snoRNP-associated protein Cic1/Utp30
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 284
Score = 25.8 bits (54), Expect = 5.4
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = +3
Query: 111 KETLKKKIKHCNHSTY 158
KETL+ ++ C HSTY
Sbjct: 165 KETLRNQVARCLHSTY 180
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 25.4 bits (53), Expect = 7.1
Identities = 21/85 (24%), Positives = 29/85 (34%)
Frame = +2
Query: 224 NLERSILLSLFGHPPKELALAVSVNQQPKPTPKAFSIHDLIGDGFLLAVPKFRRTVEKRL 403
N+ R + L GH L L S K +F ++ DG P K
Sbjct: 420 NIIRKTGIQLLGHA--NLYLTTSQQIAKKYRVVSFPKLIVVRDGIASYYPAKMAQDNKDY 477
Query: 404 NRKFGWPEYVWKPFVPKTNLKTCHE 478
R GW + W P +P+ E
Sbjct: 478 RRILGWMKNNWLPVLPELRTSNSKE 502
>SPAC12G12.05c |taf9||transcription initiation factor
Taf9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 163
Score = 25.4 bits (53), Expect = 7.1
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2
Query: 248 SLFGHPPKELALAVSVNQQPKPTPK 322
S G PPKE L +++ + KP P+
Sbjct: 85 SFTGPPPKEFLLELAMERNRKPLPQ 109
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 25.4 bits (53), Expect = 7.1
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +1
Query: 184 NVSKVHTSGPTFKKFRKIDPVKPFRSSSKRTS 279
++SKV T+GP+ I+ + P SS+R+S
Sbjct: 565 SISKVTTNGPSETISEDIEEILPSLESSERSS 596
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 25.4 bits (53), Expect = 7.1
Identities = 9/19 (47%), Positives = 10/19 (52%)
Frame = +2
Query: 458 NLKTCHECGHHHEQGRLCG 514
NL H C HHHE + G
Sbjct: 567 NLDNDHHCHHHHENHNMHG 585
>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 532
Score = 25.4 bits (53), Expect = 7.1
Identities = 10/21 (47%), Positives = 17/21 (80%)
Frame = +2
Query: 386 TVEKRLNRKFGWPEYVWKPFV 448
TV +++++K+ PEY+WKP V
Sbjct: 359 TVGRQVSKKY--PEYLWKPQV 377
>SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 765
Score = 25.0 bits (52), Expect = 9.4
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +2
Query: 158 SLRLLPILKMFPRFIQVVQHLKNLERSILLSLFGHPPKE 274
S RLLP ++P+FI +L RS L +F + P E
Sbjct: 566 SFRLLPNFTLYPQFI------FHLRRSPFLHIFNNSPDE 598
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,622,003
Number of Sequences: 5004
Number of extensions: 52910
Number of successful extensions: 122
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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