Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_B08
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53900.2 68418.m06706 expressed protein similar to unknown pr... 32 0.38
At5g53900.1 68418.m06705 expressed protein similar to unknown pr... 32 0.38
At1g01700.1 68414.m00088 expressed protein contains Pfam profile... 31 0.67
At3g07300.1 68416.m00869 eukaryotic translation initiation facto... 29 2.7
At4g37530.1 68417.m05310 peroxidase, putative similar to peroxid... 29 3.6
At3g56040.1 68416.m06226 expressed protein 29 3.6
At5g49100.1 68418.m06078 expressed protein 28 4.7
At2g33080.1 68415.m04056 leucine-rich repeat family protein cont... 28 4.7
At5g43780.1 68418.m05354 sulfate adenylyltransferase 4 / ATP-sul... 28 6.2
At4g36090.1 68417.m05137 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 6.2
At1g28350.1 68414.m03483 tRNA synthetase class I (W and Y) famil... 28 6.2
At4g37520.1 68417.m05308 peroxidase 50 (PER50) (P50) (PRXR2) ide... 27 8.2
At3g26744.1 68416.m03344 basix helix-loop-helix (bHLH) family pr... 27 8.2
At2g30690.1 68415.m03742 expressed protein contains Pfam profile... 27 8.2
>At5g53900.2 68418.m06706 expressed protein similar to unknown
protein (gb|AAF34833.1)
Length = 377
Score = 31.9 bits (69), Expect = 0.38
Identities = 30/131 (22%), Positives = 54/131 (41%), Gaps = 1/131 (0%)
Frame = +2
Query: 188 RNGLLFNNAFTSVSSCSPSRAALLTGSPSHQNGMYGLHHGVHHFNSFDNVTSLPNLLRQN 367
R+G + F+S + +PS + + P Q +G H + SF N LP R
Sbjct: 200 RSGFYLSQLFSSNRTATPSSSTVPNQIPQSQGFNWGSHSPLLPSPSFQN--QLPASARFG 257
Query: 368 GIMTGIIGKKHVGPSSVYQFDYEQTEENNHINQV-GRNITHMKLLAREFIASANKENKPF 544
+ + + + P ++ D + + N + GR +LL + N EN+
Sbjct: 258 FLQDNNVPPQMLPPMEEHEDDIKWPNGLSLFNALTGRADEASRLLFNQEQNPMNVENQNE 317
Query: 545 FLYVAFHDPHR 577
FL + H P++
Sbjct: 318 FLNLEGHHPNK 328
>At5g53900.1 68418.m06705 expressed protein similar to unknown
protein (gb|AAF34833.1)
Length = 265
Score = 31.9 bits (69), Expect = 0.38
Identities = 30/131 (22%), Positives = 54/131 (41%), Gaps = 1/131 (0%)
Frame = +2
Query: 188 RNGLLFNNAFTSVSSCSPSRAALLTGSPSHQNGMYGLHHGVHHFNSFDNVTSLPNLLRQN 367
R+G + F+S + +PS + + P Q +G H + SF N LP R
Sbjct: 88 RSGFYLSQLFSSNRTATPSSSTVPNQIPQSQGFNWGSHSPLLPSPSFQN--QLPASARFG 145
Query: 368 GIMTGIIGKKHVGPSSVYQFDYEQTEENNHINQV-GRNITHMKLLAREFIASANKENKPF 544
+ + + + P ++ D + + N + GR +LL + N EN+
Sbjct: 146 FLQDNNVPPQMLPPMEEHEDDIKWPNGLSLFNALTGRADEASRLLFNQEQNPMNVENQNE 205
Query: 545 FLYVAFHDPHR 577
FL + H P++
Sbjct: 206 FLNLEGHHPNK 216
>At1g01700.1 68414.m00088 expressed protein contains Pfam profile
PF03759: Domain of unknown function (DUF315)
Length = 485
Score = 31.1 bits (67), Expect = 0.67
Identities = 27/106 (25%), Positives = 43/106 (40%), Gaps = 1/106 (0%)
Frame = -1
Query: 351 FGRLVTLSKELKWWTPWCSPYIPFW*LGDPVRSAARLGEQLLTDVNALLNNRPFRANSSI 172
F +++ KE KWW P P +P L + R + + + + NSSI
Sbjct: 262 FRKVIVQRKEEKWWLP--IPLVPLQGLSEKARKQLKSKRESTNQI----HKAAMAINSSI 315
Query: 171 FGV*HILLRY-APISKPPSSANSKRTLRTLSR*YSFSNEKIKDKGK 37
G I Y A + K ++ R ++ FS EK+ D+ K
Sbjct: 316 LGEMDIPDSYMATLPKSGKASTGDAIYRHMTSSGRFSPEKLLDRLK 361
>At3g07300.1 68416.m00869 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|P49770 Translation initiation factor eIF-2B beta
subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF01008: Initiation factor 2
subunit family
Length = 407
Score = 29.1 bits (62), Expect = 2.7
Identities = 27/116 (23%), Positives = 52/116 (44%)
Frame = +2
Query: 239 PSRAALLTGSPSHQNGMYGLHHGVHHFNSFDNVTSLPNLLRQNGIMTGIIGKKHVGPSSV 418
PS LL G+P Y G + + +SL L+ + ++ G+ + +
Sbjct: 137 PSLQTLLEGTPESATVPYTSSSGADSESKTADKSSLTRKLKHD-VIEGV-NQLIQEIAGC 194
Query: 419 YQFDYEQTEENNHINQVGRNITHMKLLAREFIASANKENKPFFLYVAFHDPHRCGH 586
++ EQ E+ H N+V + + + EF+ +A ++ + F ++VA P GH
Sbjct: 195 HEQIAEQAIEHIHQNEVILTLGSSRTVL-EFLCAAKEKKRSFRVFVAEGAPRYQGH 249
>At4g37530.1 68417.m05310 peroxidase, putative similar to peroxidase
[Arabidopsis thaliana] gi|1402906|emb|CAA66958;
identical to Pfam profile PF00141: Peroxidase; identical
to cDNA peroxidase ATP37 GI:18874553
Length = 329
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/34 (47%), Positives = 18/34 (52%)
Frame = +2
Query: 167 PNIDELARNGLLFNNAFTSVSSCSPSRAALLTGS 268
P +D A NG LFN AF S S R + TGS
Sbjct: 285 PTVDLWANNGQLFNQAFIS-SMIKLGRVGVKTGS 317
>At3g56040.1 68416.m06226 expressed protein
Length = 883
Score = 28.7 bits (61), Expect = 3.6
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +2
Query: 224 VSSCSPSRAALLTGSPSHQNGMYGLHHGVHHFNSFDNVTSLPNLL 358
+S+ SPS L P++ N +Y H S N SLPN++
Sbjct: 531 ISNRSPSSNGLQADFPANTNILYVDLHSAELIGSSSNAKSLPNMV 575
>At5g49100.1 68418.m06078 expressed protein
Length = 396
Score = 28.3 bits (60), Expect = 4.7
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = +2
Query: 131 EIGAYRNKICQTPNIDELARNGLLFNNAFTSVSSCSPSRAALLTGSPSHQNGM 289
++G + I QT ELA + +++ +S SS R GS S++NG+
Sbjct: 165 KVGNFHQPISQTETKTELAETTTVGSSSSSSASSSMSKRVVGGGGSSSNRNGI 217
>At2g33080.1 68415.m04056 leucine-rich repeat family protein
contains leucine rich-repeat domain Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.2
[Lycopersicon pimpinellifolium] gi|1184077|gb|AAC15780
Length = 740
Score = 28.3 bits (60), Expect = 4.7
Identities = 11/37 (29%), Positives = 19/37 (51%)
Frame = +2
Query: 506 EFIASANKENKPFFLYVAFHDPHRCGHSDPQYGPFCE 616
+ +A + + + F + D HRC HSD G +C+
Sbjct: 35 DLVACRSHQIQAFTQFKNEFDTHRCNHSDHSNGVWCD 71
>At5g43780.1 68418.m05354 sulfate adenylyltransferase 4 /
ATP-sulfurylase 4 (APS4) identical to ATP sulfurylase
precursor (APS4) [Arabidopsis thaliana] GI:4633131
Length = 469
Score = 27.9 bits (59), Expect = 6.2
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +2
Query: 242 SRAALLTGSPSHQNGMYGLHHGVHHFNSFDNVTSLPNLLRQNGI 373
S AA+++GSP + + HH + +V SLP + + G+
Sbjct: 4 SAAAIVSGSPFRSSPLIHNHHASRYAPGSISVVSLPRQVSRRGL 47
>At4g36090.1 68417.m05137 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to At2g17970, At2g17960; contains
PF03171 2OG-Fe(II) oxygenase superfamily domain
Length = 385
Score = 27.9 bits (59), Expect = 6.2
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = +2
Query: 143 YRNKICQTPNIDELARNGLLFNNAFTSVSSCSPSRAALLTGSPSHQ 280
Y K+C + ++D L +F + SVS PS + ++ + S Q
Sbjct: 94 YDGKLCVSHSLDSLKAGAFVFGSLSDSVSHAMPSSQSTVSEAASAQ 139
>At1g28350.1 68414.m03483 tRNA synthetase class I (W and Y) family
protein contains Pfam profile: PF00579 tRNA synthetases
class I (W and Y)
Length = 824
Score = 27.9 bits (59), Expect = 6.2
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +2
Query: 458 INQVGRNITHMKLLAREFIASANKENKPFFL 550
I Q+G + + +LARE+ A ++NKP L
Sbjct: 655 ICQLGMDQRKVNMLAREYCADIKRKNKPIIL 685
>At4g37520.1 68417.m05308 peroxidase 50 (PER50) (P50) (PRXR2)
identical to SP|Q43731 Peroxidase 50 precursor (EC
1.11.1.7) (Atperox P50) (PRXR2) (ATP9a)] {Arabidopsis
thaliana}
Length = 329
Score = 27.5 bits (58), Expect = 8.2
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = +2
Query: 167 PNIDELARNGLLFNNAFTSVSSCSPSRAALLTGS 268
P +D A NG LFN AF + S R + TGS
Sbjct: 285 PTVDLWANNGQLFNQAFIN-SMIKLGRVGVKTGS 317
>At3g26744.1 68416.m03344 basix helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 494
Score = 27.5 bits (58), Expect = 8.2
Identities = 15/31 (48%), Positives = 19/31 (61%)
Frame = +2
Query: 191 NGLLFNNAFTSVSSCSPSRAALLTGSPSHQN 283
+ LL ++ S SSCSPS+A L PS QN
Sbjct: 79 DNLLLQHSIDSSSSCSPSQAFSL--DPSQQN 107
>At2g30690.1 68415.m03742 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593; expression
supported by MPSS
Length = 788
Score = 27.5 bits (58), Expect = 8.2
Identities = 21/57 (36%), Positives = 27/57 (47%), Gaps = 6/57 (10%)
Frame = +2
Query: 131 EIGAYRNKICQTPNIDELARNGLLFNNAFTSVSS------CSPSRAALLTGSPSHQN 283
E+ YR K P + LA G+L N T+V+S S LTGSPS +N
Sbjct: 732 ELEYYRVKYPDEPREEILASMGILGNTEETNVTSPTDETSIKDSTDTKLTGSPSAEN 788
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,392,221
Number of Sequences: 28952
Number of extensions: 268506
Number of successful extensions: 814
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 787
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 814
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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