Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_A23
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces... 299 3e-82
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 27 1.8
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 3.1
SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 25 7.2
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 25 9.5
SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb... 25 9.5
SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 25 9.5
SPBC25H2.15 |||programmed cell death protein homolog|Schizosacch... 25 9.5
>SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 249
Score = 299 bits (733), Expect = 3e-82
Identities = 146/203 (71%), Positives = 164/203 (80%)
Frame = +2
Query: 35 AVNNISKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLG 214
A ISKKRKFV DGVF AELNEF TREL+E+GYSG EVRVTP RSEIII AT TQ VLG
Sbjct: 3 AAFTISKKRKFVADGVFYAELNEFFTRELSEEGYSGCEVRVTPSRSEIIIRATHTQDVLG 62
Query: 215 EKGRRIRELTSVVQKRFNIPEQSVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACY 394
EKGRRIRELT++VQKRF E +VELYAEKV RGLCA+AQ ESLRYKL+ GLAVRRA Y
Sbjct: 63 EKGRRIRELTALVQKRFKFAENTVELYAEKVQNRGLCAVAQCESLRYKLLAGLAVRRAAY 122
Query: 395 GVLRFIMESGARGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPCNDYVNTAXRHVLLRQG 574
GVLR++ME+GA+GCEVV+SGKLR RAKSMKF DG MIHSG P D++++A RHVLLRQG
Sbjct: 123 GVLRYVMEAGAKGCEVVISGKLRAARAKSMKFADGFMIHSGQPAVDFIDSATRHVLLRQG 182
Query: 575 VLGIKVKSCCRGTAGRRPKITPD 643
VLG+KVK R+ K PD
Sbjct: 183 VLGVKVKIMLPEPKTRQKKSLPD 205
>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2111
Score = 27.5 bits (58), Expect = 1.8
Identities = 15/52 (28%), Positives = 26/52 (50%)
Frame = +2
Query: 401 LRFIMESGARGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPCNDYVNTAXRH 556
+ F ME + + ++ +L+ S K D ++I GD ND + T+ RH
Sbjct: 1868 IHFAMEFLHKNTKSLLDSELKDGNYISAKGKDVIVIGGGDTGNDCLGTSVRH 1919
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.6 bits (56), Expect = 3.1
Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
Frame = +2
Query: 185 MATRTQSVLGEKGRRIRELTSVVQKRFNIPEQSVELYAEKVATRGLCAI-AQAESLRYKL 361
MATR Q RR E + +QK +N + ++EL E+V G + + + RY +
Sbjct: 726 MATRIQRAWRSYVRRRSEAAACIQKLWNRNKVNMEL--ERVRNEGTKLLQGKKQRRRYSI 783
Query: 362 IG 367
+G
Sbjct: 784 LG 785
>SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 471
Score = 25.4 bits (53), Expect = 7.2
Identities = 22/70 (31%), Positives = 28/70 (40%), Gaps = 2/70 (2%)
Frame = -2
Query: 499 SIYKLH*FGTLTTQLARYNNFTTTGARFHDETENTIASTTYSETSDKLVS*RFGLG--DS 326
S+Y + T+ LA+YN E I+ST S V R LG
Sbjct: 296 SVYSTMAYAEATSNLAKYNTIAFGNCLDEKFEEEIISSTARSFFLGDEVKKRLLLGAYSL 355
Query: 325 AKTTSSHLFS 296
A+ SS LFS
Sbjct: 356 ARMNSSDLFS 365
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 25.0 bits (52), Expect = 9.5
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = -3
Query: 303 FSAYNSTDCSGMLNRFCTTEVSSR 232
+ +Y S+DC G+L+R T+ R
Sbjct: 360 YPSYLSSDCKGLLSRMLVTDPLKR 383
>SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +2
Query: 464 GQRAKSMKFVDGLMIHSGDPCNDYVN 541
G+R K + F D H G P DY++
Sbjct: 218 GRREKLLVFGDDYDSHDGTPIRDYIH 243
>SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 878
Score = 25.0 bits (52), Expect = 9.5
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2
Query: 289 FYRLLWNVESLLYYGSQL 236
FYRL W + +LY SQ+
Sbjct: 643 FYRLCWKDKGILYQDSQI 660
>SPBC25H2.15 |||programmed cell death protein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 396
Score = 25.0 bits (52), Expect = 9.5
Identities = 15/53 (28%), Positives = 23/53 (43%)
Frame = -3
Query: 363 ISLYLRDSAWAIAQRPRVATFSAYNSTDCSGMLNRFCTTEVSSRILRPFSPST 205
+ L L+ A+ ++ S+ +N F T SSR L PFS +T
Sbjct: 101 VRLPLKSDIEAVKSPKAISHLEEKKSSPKEKKVNPFAITSESSRGLNPFSDAT 153
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,785,596
Number of Sequences: 5004
Number of extensions: 56179
Number of successful extensions: 122
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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