Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_A19
(655 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g05020.1 68417.m00736 NADH dehydrogenase-related similar to a... 31 0.67
At1g78430.1 68414.m09139 tropomyosin-related similar to Tropomyo... 30 1.2
At5g46630.2 68418.m05740 clathrin adaptor complexes medium subun... 29 2.0
At5g46630.1 68418.m05741 clathrin adaptor complexes medium subun... 29 2.0
At5g27330.1 68418.m03263 expressed protein 29 2.7
At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEME... 29 3.6
At3g01175.1 68416.m00022 expressed protein 29 3.6
At2g07770.1 68415.m01005 hypothetical protein low similarity to ... 29 3.6
At5g27630.1 68418.m03310 acyl-CoA binding family protein similar... 28 6.2
At4g09780.1 68417.m01607 meprin and TRAF homology domain-contain... 28 6.2
At1g79830.1 68414.m09326 expressed protein weak similarity to TA... 28 6.2
At5g65450.1 68418.m08231 ubiquitin carboxyl-terminal hydrolase f... 27 8.2
At1g69630.1 68414.m08010 F-box family protein contains F-box dom... 27 8.2
At1g03310.2 68414.m00310 isoamylase, putative / starch debranchi... 27 8.2
At1g03310.1 68414.m00309 isoamylase, putative / starch debranchi... 27 8.2
>At4g05020.1 68417.m00736 NADH dehydrogenase-related similar to
alternative NADH-dehydrogenase [Yarrowia lipolytica]
GI:3718005, 64 kDa mitochondrial NADH dehydrogenase
[Neurospora crassa] GI:4753821; contains Pfam profile
PF00070: Pyridine nucleotide-disulphide oxidoreductase
Length = 582
Score = 31.1 bits (67), Expect = 0.67
Identities = 21/90 (23%), Positives = 42/90 (46%), Gaps = 2/90 (2%)
Frame = +2
Query: 275 VFYDSENDVD-SLDYEHLNRAMIDECKEYDKMDKXLLKLNRAKVQTMCLKTEKGKLSKEN 451
+F ++ D +L + AM D C Y +++ L + + + E +SK N
Sbjct: 387 IFSKADKDKSGTLTLKEFQEAMDDICVRYPQVELYLKSKRMRGIADLLKEAETDDVSKNN 446
Query: 452 IQLK-HYIKKYLTELALREKDRPHSVKIAS 538
I+LK K L+++ + K P + ++A+
Sbjct: 447 IELKIEEFKSALSQVDSQVKFLPATAQVAA 476
>At1g78430.1 68414.m09139 tropomyosin-related similar to Tropomyosin
1 [Baker's yeast](SP:P17536) {Saccharomyces cerevisiae};
similar to enterophilin-2L (GI:12718845) [Cavia
porcellus]; similar to latent nuclear antigen
(GI:5669894) [Human herpesvirus 8]; similar to multiple
ligand-binding protein 1 (GI:1403575) [Streptococcus
sp.]
Length = 326
Score = 30.3 bits (65), Expect = 1.2
Identities = 26/113 (23%), Positives = 56/113 (49%), Gaps = 4/113 (3%)
Frame = +2
Query: 329 RAMIDECKEYDKMDKXLLKLNRAKVQTMCLKTEKGKLSKENIQLKHYIKKYLTELA-LRE 505
+A E + D++ ++N K + L+ E+ LS+EN LK +KK TE++ +
Sbjct: 142 KAKESEKTKNDELASKEDQINVLKARLYDLEKERVSLSEENETLKDQLKKTDTEMSCAKA 201
Query: 506 KDRPHSVKIASGVQKLDANGKT---LNRPVTCIEGALCNAVMHEKRMKTYVKK 655
K+ + K++ ++L+ + +T L + + +E A K++K ++
Sbjct: 202 KEDEIASKVSQIGEELEESNETTAKLKKKLESVEEAKETLEAEMKKLKVQTEQ 254
>At5g46630.2 68418.m05740 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 441
Score = 29.5 bits (63), Expect = 2.0
Identities = 21/79 (26%), Positives = 35/79 (44%)
Frame = -1
Query: 343 VNHCSIQMFIVKRIHIILGVIKNNVIADVFVFTANISQLNYFIYFLFHFFQPLKVYIRSN 164
+ CS + ++I++ V N +A F F L + YF F + IR+N
Sbjct: 53 IGGCSFVYMRISNVYIVIVVSSNANVACGFKFVVEAVAL-FKSYFGGAFDEDA---IRNN 108
Query: 163 IIMVYELLNSTYLNSYENN 107
+++YELL+ Y N
Sbjct: 109 FVLIYELLDEIMDFGYPQN 127
>At5g46630.1 68418.m05741 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 438
Score = 29.5 bits (63), Expect = 2.0
Identities = 21/79 (26%), Positives = 35/79 (44%)
Frame = -1
Query: 343 VNHCSIQMFIVKRIHIILGVIKNNVIADVFVFTANISQLNYFIYFLFHFFQPLKVYIRSN 164
+ CS + ++I++ V N +A F F L + YF F + IR+N
Sbjct: 53 IGGCSFVYMRISNVYIVIVVSSNANVACGFKFVVEAVAL-FKSYFGGAFDEDA---IRNN 108
Query: 163 IIMVYELLNSTYLNSYENN 107
+++YELL+ Y N
Sbjct: 109 FVLIYELLDEIMDFGYPQN 127
>At5g27330.1 68418.m03263 expressed protein
Length = 628
Score = 29.1 bits (62), Expect = 2.7
Identities = 19/63 (30%), Positives = 36/63 (57%), Gaps = 2/63 (3%)
Frame = +2
Query: 416 LKTEKGKLSKENIQLKHYIKKYLTELALREKDRPHSVKIASGVQK--LDANGKTLNRPVT 589
+K E+G+L KE +L++ + E A+ +KD +K A+GV K L++ G + +T
Sbjct: 478 IKAERGRLIKEKKELENRSESLRNEKAILQKDIV-ELKRATGVLKTELESAGTNAKQSLT 536
Query: 590 CIE 598
++
Sbjct: 537 MLK 539
>At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEMETER
protein [Arabidopsis thaliana] GI:21743571; contains Pfam
profile PF00730: HhH-GPD superfamily base excision DNA
repair protein
Length = 1729
Score = 28.7 bits (61), Expect = 3.6
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +2
Query: 287 SENDVDSLDYEHLNRAMIDECKE 355
++N++DS+DYE + RA I E E
Sbjct: 1209 NKNNMDSIDYEAIRRASISEISE 1231
>At3g01175.1 68416.m00022 expressed protein
Length = 401
Score = 28.7 bits (61), Expect = 3.6
Identities = 31/134 (23%), Positives = 64/134 (47%), Gaps = 6/134 (4%)
Frame = +2
Query: 155 HNYIRS-NVNFQRLEEVKQKINKVIQLADICRKYENVGDNIVFYDSENDVDSLDYEHLNR 331
HN + + + +F+ + K+ +Q I + E+V D YD E+ V+ LD +
Sbjct: 110 HNPVPAFSFSFKYQVDSHLKVRISMQKGKILEEEEDVDDFFYEYDQEDVVELLDAKE--- 166
Query: 332 AMIDECKEYDKMDKXLLKLN-RAKVQTMCLKTE-KGKLSKENIQ---LKHYIKKYLTELA 496
++E + D D ++ N ++QTM + E + L+ ++ + + ++ L+ L
Sbjct: 167 --VEEEHDVDIFDGVNMEKNLEFEIQTMPIHQEDEASLTNDDEEEEIVNSDVENDLSFLV 224
Query: 497 LREKDRPHSVKIAS 538
++D P + IAS
Sbjct: 225 SSKRDHPSPLSIAS 238
>At2g07770.1 68415.m01005 hypothetical protein low similarity to KED
[Nicotiana tabacum] GI:8096269; contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 518
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/33 (36%), Positives = 23/33 (69%)
Frame = +2
Query: 314 YEHLNRAMIDECKEYDKMDKXLLKLNRAKVQTM 412
++ +N+ M D CK+Y++ DK LKL A ++++
Sbjct: 257 FKSMNKRMKDFCKKYEEQDKR-LKLMEAAIKSI 288
>At5g27630.1 68418.m03310 acyl-CoA binding family protein similar to
RING finger rngB protein, cytosolic - Dictyostelium
discoideum, PIR:S68824; contains Pfam profiles PF01344:
Kelch motif, PF00887: Acyl CoA binding protein (ACBP)
Length = 648
Score = 27.9 bits (59), Expect = 6.2
Identities = 19/72 (26%), Positives = 37/72 (51%), Gaps = 1/72 (1%)
Frame = +2
Query: 386 LNRAKVQTMCLKTEKGKLSKENIQLKHYIKKYLTELALRE-KDRPHSVKIASGVQKLDAN 562
LN+ K+QT+ LK E ++ N +L ++ +LA + + V++A QKL
Sbjct: 543 LNKEKIQTLQLKEELAEIDTRNTELYKELQSVRNQLAAEQSRCFKLEVEVAELRQKLQTM 602
Query: 563 GKTLNRPVTCIE 598
+TL + + ++
Sbjct: 603 -ETLQKELELLQ 613
>At4g09780.1 68417.m01607 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein low similarity
to ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 443
Score = 27.9 bits (59), Expect = 6.2
Identities = 18/62 (29%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
Frame = -1
Query: 232 QLNYFIYFLFHFFQPLKV--YIRSNIIMVYELLNSTYLNSYENN*LLFS*GITCTNELKI 59
Q ++F+Y +FHFF K Y S +I V E LN + ++ + C E K+
Sbjct: 316 QTSFFLYLVFHFFSREKEDGYGTSELISV-EDLNDESKGYLVEDTIVLETTLLCVTETKV 374
Query: 58 EI 53
++
Sbjct: 375 KL 376
>At1g79830.1 68414.m09326 expressed protein weak similarity to TATA
element modulatory factor (TMF) (Swiss-Prot:P82094)
[Homo sapiens]
Length = 927
Score = 27.9 bits (59), Expect = 6.2
Identities = 10/29 (34%), Positives = 20/29 (68%)
Frame = +2
Query: 359 DKMDKXLLKLNRAKVQTMCLKTEKGKLSK 445
+++ + L ++N + Q CL+ E+G+LSK
Sbjct: 671 ERLSQTLSRINVLEAQLSCLRAEQGQLSK 699
>At5g65450.1 68418.m08231 ubiquitin carboxyl-terminal hydrolase
family protein / zinc finger (MYND type) family protein
similar to ubiquitin-specific protease 16 (UBP16)
[Arabidopsis thaliana] GI:11993477; contains Pfam
profiles PF00443: Ubiquitin carboxyl-terminal hydrolase,
PF01753: MYND finger
Length = 731
Score = 27.5 bits (58), Expect = 8.2
Identities = 17/44 (38%), Positives = 21/44 (47%), Gaps = 4/44 (9%)
Frame = +2
Query: 326 NRAMIDECKEYDKMDKXLLKLNRAKVQTMCLKTEK----GKLSK 445
NR CK Y K K L+ L + T+ LK + GKLSK
Sbjct: 505 NRYFCGRCKSYQKAKKKLMILEGPNILTVVLKRFQSDNFGKLSK 548
>At1g69630.1 68414.m08010 F-box family protein contains F-box domain
Pfam:PF00646
Length = 451
Score = 27.5 bits (58), Expect = 8.2
Identities = 15/56 (26%), Positives = 24/56 (42%)
Frame = +2
Query: 251 YENVGDNIVFYDSENDVDSLDYEHLNRAMIDECKEYDKMDKXLLKLNRAKVQTMCL 418
Y+ G+NI+ + SL+Y + R + E E + L K T+CL
Sbjct: 366 YQEKGENIILPGPRRFLSSLEYVKIERPLKGEAMEMKLVSYLLENSTILKKLTLCL 421
>At1g03310.2 68414.m00310 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase isoform 2
[Solanum tuberosum] GI:27728147, isoamylase from
[Triticum aestivum] GI:17932898, [Hordeum vulgare]
GI:21314275, [Oryza sativa] GI:3252794; contains Pfam
profiles PF00128: Alpha amylase catalytic domain,
PF02922: Isoamylase N-terminal domain; ESTs gb|H36690,
gb|AA712462, gb|AA651230 and gb|N95932 come from this
gene
Length = 882
Score = 27.5 bits (58), Expect = 8.2
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1
Query: 214 YFLFHFFQPLKVYIRSN 164
YF FHFF P+ +Y SN
Sbjct: 423 YFPFHFFSPMDIYGPSN 439
>At1g03310.1 68414.m00309 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase isoform 2
[Solanum tuberosum] GI:27728147, isoamylase from
[Triticum aestivum] GI:17932898, [Hordeum vulgare]
GI:21314275, [Oryza sativa] GI:3252794; contains Pfam
profiles PF00128: Alpha amylase catalytic domain,
PF02922: Isoamylase N-terminal domain; ESTs gb|H36690,
gb|AA712462, gb|AA651230 and gb|N95932 come from this
gene
Length = 882
Score = 27.5 bits (58), Expect = 8.2
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1
Query: 214 YFLFHFFQPLKVYIRSN 164
YF FHFF P+ +Y SN
Sbjct: 423 YFPFHFFSPMDIYGPSN 439
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,610,597
Number of Sequences: 28952
Number of extensions: 242892
Number of successful extensions: 714
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 714
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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