Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_A16
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1751.03 ||SPAC31A2.01|translation initiation factor eIF3m|Sc... 42 8e-05
SPAC25G10.08 |||translation initiation factor eIF3b |Schizosacch... 29 0.77
SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces p... 29 0.77
SPAC6F6.08c |cdc16|bub2|two-component GAP Cdc16|Schizosaccharomy... 27 3.1
SPAC27F1.04c |nuf2||spindle pole body protein Nuf2|Schizosacchar... 26 5.5
SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16 |Schizosa... 25 7.2
SPCC1919.01 |ppk34|SPCC830.12|serine/threonine protein kinase Pp... 25 7.2
SPAC1751.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 9.5
>SPAC1751.03 ||SPAC31A2.01|translation initiation factor
eIF3m|Schizosaccharomyces pombe|chr 1|||Manual
Length = 402
Score = 41.9 bits (94), Expect = 8e-05
Identities = 36/152 (23%), Positives = 73/152 (48%), Gaps = 9/152 (5%)
Frame = +3
Query: 219 LEQFVLLAKGAKGSACAELIKQVLEAPGVHVFGELLEMPNIKELESGPYATHLKTLNLFA 398
L + V + A EL+++ + +P F +++ +P +++LE L+ L + +
Sbjct: 196 LLEAVKMDNSTADEAVRELVQRAVNSPKYFFFDDIVTLPPVQQLEQST----LQLLGILS 251
Query: 399 YGTYKDYI----ENKSDYLELTTVQ---CKKLQHLTIATLATQ--EKCIPYSVLLEELDI 551
G DY+ EN + + +K++ LTIA+LATQ + Y + + L I
Sbjct: 252 GGMTDDYVSWVAENHAHCQHQKFDEDAIARKMKLLTIASLATQAPNNTLSYGDVAKSLKI 311
Query: 552 KNVRDLEDLIIEAXYADIIHGKLDQECKRVEV 647
+ ++E II+ A ++ G++ Q K + +
Sbjct: 312 -DENEVELWIIDVIRAGLVEGRMSQLTKTLSI 342
>SPAC25G10.08 |||translation initiation factor eIF3b
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 725
Score = 28.7 bits (61), Expect = 0.77
Identities = 25/84 (29%), Positives = 40/84 (47%), Gaps = 3/84 (3%)
Frame = +3
Query: 318 ELLEMPNIKELESGPYATHLKTLNLFAYGTYKDYIENKSDYLELTTVQCKKLQHLTIATL 497
E+ P L S P + ++T NLF K Y ++ DYL + + K + T + L
Sbjct: 379 EITNQPARVALISIPSKSTIRTKNLFNVSDCKLYWQSNGDYLCVKVDRHTKTKKSTFSNL 438
Query: 498 ---ATQEKCIPYSVLLEELDIKNV 560
+EK IP +E +D+K+V
Sbjct: 439 EIFRIREKNIP----VEVVDLKDV 458
>SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 28.7 bits (61), Expect = 0.77
Identities = 18/63 (28%), Positives = 27/63 (42%), Gaps = 1/63 (1%)
Frame = +3
Query: 363 YATHLKTLNLFAYGTYKDYIENKSDYLELTTVQCKKLQHLTIATLATQEKCI-PYSVLLE 539
YA + YK Y+E +SDY EL K+L + + + CI S L+
Sbjct: 161 YAKSAHSTKTVVDALYKSYLEKQSDYSELLDNYKKQLNKASESCKGNYQACIESRSTLIT 220
Query: 540 ELD 548
L+
Sbjct: 221 SLE 223
>SPAC6F6.08c |cdc16|bub2|two-component GAP Cdc16|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 299
Score = 26.6 bits (56), Expect = 3.1
Identities = 12/37 (32%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
Frame = +3
Query: 447 LTTVQCKKLQHLTIATLATQEKCIP---YSVLLEELD 548
+ ++ CK+LQ L + Q KCI Y V+L++++
Sbjct: 1 MPSIDCKRLQKLAPKSPENQSKCISRLRYMVMLDQVE 37
>SPAC27F1.04c |nuf2||spindle pole body protein
Nuf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 5.5
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = +3
Query: 441 LELTTVQCKKLQHLTIATLATQEKCIPYSVLLEELDIKNVRDLEDL 578
LE V+ KL+H T+ QE C + ++L+ + + L+ L
Sbjct: 295 LEECLVELDKLEHATVLLSTNQELCDQIEINKKKLEFRKEQLLKQL 340
>SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/27 (40%), Positives = 19/27 (70%)
Frame = -2
Query: 425 LNVVLICAIGKQIKSF*MSCIRSTLQL 345
LN+ + C I + I+SF +S +RST ++
Sbjct: 491 LNLNVCCCIAEPIQSFILSFLRSTYKV 517
>SPCC1919.01 |ppk34|SPCC830.12|serine/threonine protein kinase
Ppk34|Schizosaccharomyces pombe|chr 3|||Manual
Length = 354
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +3
Query: 495 LATQEKCIPYSVLLEELDIKNVRDLEDLI 581
+ QEK PY + +ELD+ V D E+++
Sbjct: 106 IRNQEKKDPYYYIRKELDVLKVLDHENVV 134
>SPAC1751.04 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 123
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +3
Query: 312 FGELLEMPNIKELESGPYATHLKTLNLFAYGTYKDYIENK 431
F + + NIK+ +S + +N+ Y +YK Y NK
Sbjct: 7 FTQQVIFKNIKQQKSQVSVVDVTMINISLYKSYKSYPTNK 46
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,566,066
Number of Sequences: 5004
Number of extensions: 49430
Number of successful extensions: 134
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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