Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_A12
(653 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g49510.1 68418.m06127 VHL binding protein, putative / prefold... 103 1e-22
At1g06530.1 68414.m00692 myosin heavy chain-related similar to m... 43 2e-04
At5g62250.1 68418.m07816 microtubule associated protein (MAP65/A... 34 0.072
At2g25140.1 68415.m03007 heat shock protein 100, putative / HSP1... 33 0.13
At1g68790.1 68414.m07863 expressed protein 33 0.22
At3g53260.1 68416.m05870 phenylalanine ammonia-lyase 2 (PAL2) ne... 31 0.88
At2g37040.1 68415.m04544 phenylalanine ammonia-lyase 1 (PAL1) ne... 30 1.5
At5g06060.1 68418.m00671 tropinone reductase, putative / tropine... 29 2.0
At1g75150.1 68414.m08729 expressed protein ; expression supporte... 29 2.0
At1g63300.1 68414.m07156 expressed protein similar to Intracellu... 29 2.0
At1g61290.1 68414.m06908 syntaxin, putative (SYP124) similar to ... 29 2.0
At3g53120.1 68416.m05854 expressed protein 29 2.7
At3g10340.1 68416.m01240 phenylalanine ammonia-lyase, putative s... 29 2.7
At3g47060.1 68416.m05110 FtsH protease, putative contains simila... 29 3.6
At2g29330.1 68415.m03562 tropinone reductase, putative / tropine... 28 4.7
At1g60200.1 68414.m06781 splicing factor PWI domain-containing p... 28 6.2
At1g10950.1 68414.m01257 endomembrane protein 70, putative 28 6.2
At3g19720.2 68416.m02498 dynamin family protein identical to cDN... 27 8.2
At3g19720.1 68416.m02497 dynamin family protein identical to cDN... 27 8.2
At1g30190.1 68414.m03690 expressed protein ; expression supporte... 27 8.2
>At5g49510.1 68418.m06127 VHL binding protein, putative / prefoldin,
putative similar to Swiss-Prot:Q15765 prefoldin subunit
3 (Von Hippel-Lindau binding protein 1; VHL binding
protein-1; VBP-1; HIBBJ46) [Mus musculus]
Length = 195
Score = 103 bits (246), Expect = 1e-22
Identities = 57/146 (39%), Positives = 90/146 (61%), Gaps = 5/146 (3%)
Frame = +1
Query: 229 GIPEAEFVDNVDEFMKSPINAEGVDV--VLKSLDEKHRKYKVMEYTLATKRRRLRQQIPD 402
GIP A+F+ +V+ ++ G+D L E+ ++YKV+E L ++R L+ +IPD
Sbjct: 18 GIPAAKFIQDVETYLSQ----SGLDPNSALAFHQERLQQYKVVEMKLLAQQRDLQAKIPD 73
Query: 403 LARTIEVIEKLKEQK---EEVETQFLLSDQVFVKANVPPTKSVCLWLGANVMLEYSLEDA 573
+ + +EV+ L+ +K E + F +S+ ++ +A + T SVCLWLGANVMLEYS E+A
Sbjct: 74 IEKCLEVVATLEAKKGTGEALLADFEVSEGIYSRACIEDTDSVCLWLGANVMLEYSCEEA 133
Query: 574 EKLLTTNMETAQENLNQVEHDLDFLR 651
LL N+E A+ +L + DL FLR
Sbjct: 134 SALLKNNLENAKASLEVLVADLQFLR 159
>At1g06530.1 68414.m00692 myosin heavy chain-related similar to
myosin heavy chain (GI:1408194) {Placopecten
magellanicus}; similar to Myosin heavy chain, clone 203
(Fragment) (SP:P39922){Hydra attenuata}; contains one
transmembrane domain
Length = 323
Score = 42.7 bits (96), Expect = 2e-04
Identities = 36/152 (23%), Positives = 78/152 (51%), Gaps = 1/152 (0%)
Frame = +1
Query: 178 IKMEGDGVEPSNPKSYSGIPEAEFVDNVDEFMKSPINAEGVDVVLKSLDEKHRKYKVMEY 357
I +G G+E K +G+ + + ++ MK + G + +K LDEK++K++ E
Sbjct: 139 ISQKGGGIEELE-KEVAGLRTVK--EENEKRMKELESKLGA-LEVKELDEKNKKFRAEE- 193
Query: 358 TLATKRRRLRQQIPDLARTIEVIEKLKEQKEEVETQFLLSDQVFVKANVPPTKSVCLWLG 537
+ K +++ DL I+ +E K + E Q +++++ V+ ++ ++ + L
Sbjct: 194 EMREKIDNKEKEVHDLKEKIKSLES-DVAKGKTELQKWITEKMVVEDSLKDSEKKVVALE 252
Query: 538 ANVM-LEYSLEDAEKLLTTNMETAQENLNQVE 630
+ ++ L+ L+DAEK++ +E LN +E
Sbjct: 253 SEIVELQKQLDDAEKMINGLKNVVEEPLNGIE 284
>At5g62250.1 68418.m07816 microtubule associated protein
(MAP65/ASE1) family protein low similarity to protein
regulating cytokinesis 1 (PRC1) [Homo sapiens]
GI:2865521; contains Pfam profile PF03999: Microtubule
associated protein (MAP65/ASE1 family)
Length = 549
Score = 34.3 bits (75), Expect = 0.072
Identities = 20/53 (37%), Positives = 30/53 (56%)
Frame = +1
Query: 262 DEFMKSPINAEGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIE 420
DE S +A+ D+ L+ L+E HR+ YTL ++R +QI D RT+E
Sbjct: 145 DETCSSDFSADESDLSLRKLEELHREL----YTLQEQKRNRVKQIQDNIRTLE 193
>At2g25140.1 68415.m03007 heat shock protein 100, putative / HSP100,
putative / heat shock protein clpB, putative /
HSP100/ClpB, putative similar to HSP100/ClpB GI:9651530
[Phaseolus lunatus]
Length = 964
Score = 33.5 bits (73), Expect = 0.13
Identities = 31/130 (23%), Positives = 64/130 (49%), Gaps = 2/130 (1%)
Frame = +1
Query: 259 VDEFMKSPINAEGVDVVLKSLDEKHRKYKV--MEYTLATKRRRLRQQIPDLARTIEVIEK 432
+D ++ I E + LK+ +K K ++ +E L+T +++ ++ + ++ K
Sbjct: 493 LDGIDRAVIKLEMEKLSLKNDTDKASKERLQKIENDLSTLKQKQKELNVQWEKEKSLMTK 552
Query: 433 LKEQKEEVETQFLLSDQVFVKANVPPTKSVCLWLGANVMLEYSLEDAEKLLTTNMETAQE 612
++ KEE++ L + + ++ ++ L G + L+ LE+AEK LT + Q
Sbjct: 553 IRSFKEEIDRVNLEIESAEREYDL--NRAAELKYGTLLSLQRQLEEAEKNLTNFRQFGQS 610
Query: 613 NLNQVEHDLD 642
L +V DLD
Sbjct: 611 LLREVVTDLD 620
>At1g68790.1 68414.m07863 expressed protein
Length = 1085
Score = 32.7 bits (71), Expect = 0.22
Identities = 18/58 (31%), Positives = 29/58 (50%)
Frame = +1
Query: 292 EGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIEVIEKLKEQKEEVETQ 465
EGV K LD + + K E L + ++L + L E + KLK++ EE+ T+
Sbjct: 415 EGVKKKEKDLDARLKTVKEKEKALKAEEKKLHMENERLLEDKECLRKLKDEIEEIGTE 472
>At3g53260.1 68416.m05870 phenylalanine ammonia-lyase 2 (PAL2)
nearly identical to SP|P45724
Length = 717
Score = 30.7 bits (66), Expect = 0.88
Identities = 18/50 (36%), Positives = 27/50 (54%)
Frame = -1
Query: 293 SALIGDFINSSTLSTNSASGIPEYDLGFEGSTPSPSIFIYCEVYIQFLEN 144
S L+ DF N+ S +AS P D GF+G+ ++ YC +Q+L N
Sbjct: 429 SELVNDFYNNGLPSNLTASSNPSLDYGFKGA--EIAMASYCS-ELQYLAN 475
>At2g37040.1 68415.m04544 phenylalanine ammonia-lyase 1 (PAL1)
nearly identical to SP|P35510
Length = 725
Score = 29.9 bits (64), Expect = 1.5
Identities = 18/50 (36%), Positives = 27/50 (54%)
Frame = -1
Query: 293 SALIGDFINSSTLSTNSASGIPEYDLGFEGSTPSPSIFIYCEVYIQFLEN 144
S L+ DF N+ S +AS P D GF+G+ ++ YC +Q+L N
Sbjct: 437 SELVNDFYNNGLPSNLTASRNPSLDYGFKGA--EIAMASYCS-ELQYLAN 483
>At5g06060.1 68418.m00671 tropinone reductase, putative / tropine
dehydrogenase, putative similar to tropinone reductase
SP:P50165 from [Datura stramonium]
Length = 264
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/39 (41%), Positives = 27/39 (69%), Gaps = 3/39 (7%)
Frame = +1
Query: 532 LGANVM---LEYSLEDAEKLLTTNMETAQENLNQVEHDL 639
+G NV +EYS E+ K+++TN+E+A +L+Q+ H L
Sbjct: 97 VGTNVRKPTVEYSSEEYAKIMSTNLESA-FHLSQIAHPL 134
>At1g75150.1 68414.m08729 expressed protein ; expression supported
by MPSS
Length = 753
Score = 29.5 bits (63), Expect = 2.0
Identities = 18/72 (25%), Positives = 34/72 (47%), Gaps = 2/72 (2%)
Frame = +1
Query: 262 DEFMKSPINAEGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLART--IEVIEKL 435
D+F + P++ +++ K E + + L + R + L R V+EK+
Sbjct: 197 DDFDELPVSTASMNMTKKERREYLDQLRAENQRLLRETRDAAFEAAPLVRKPISSVLEKI 256
Query: 436 KEQKEEVETQFL 471
+ +KEE+ QFL
Sbjct: 257 RRRKEEISKQFL 268
>At1g63300.1 68414.m07156 expressed protein similar to Intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]; similar to Myosin II heavy
chain, non muscle (Swiss-Prot:P08799) [Dictyostelium
discoideum]
Length = 1029
Score = 29.5 bits (63), Expect = 2.0
Identities = 22/86 (25%), Positives = 40/86 (46%)
Frame = +1
Query: 244 EFVDNVDEFMKSPINAEGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIEV 423
E DN++E M+ +E + D+K + V ++ A L Q+I DL IE+
Sbjct: 424 EGADNIEESMRRSCRSETDE---DDHDQKALEDLVKKHVDAKDTHILEQKITDLYNEIEI 480
Query: 424 IEKLKEQKEEVETQFLLSDQVFVKAN 501
++ K++ E Q L ++ + N
Sbjct: 481 YKRDKDELEIQMEQLALDYEILKQQN 506
>At1g61290.1 68414.m06908 syntaxin, putative (SYP124) similar to
syntaxin-related protein Nt-syr1 GI:4206787 from
[Nicotiana tabacum]
Length = 303
Score = 29.5 bits (63), Expect = 2.0
Identities = 14/58 (24%), Positives = 32/58 (55%)
Frame = +1
Query: 286 NAEGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIEVIEKLKEQKEEVE 459
N +GV+ + KSL + + + K + K R + D+A+ ++ ++ +K++ E +E
Sbjct: 46 NMKGVETLYKSLQDSNEECKTVHNAKKVKELRAKMD-GDVAQVLKRVKMIKQKLEALE 102
>At3g53120.1 68416.m05854 expressed protein
Length = 217
Score = 29.1 bits (62), Expect = 2.7
Identities = 26/81 (32%), Positives = 43/81 (53%), Gaps = 7/81 (8%)
Frame = +1
Query: 409 RTIEVIEKLKEQKEEVETQFLLS-DQVFVKANVPPT-KSVCLWLGA-NVMLEYSLEDAEK 579
++++ + KL K+ + QFLLS DQV V+ N+ + L L N+ E + +
Sbjct: 65 KSVDELRKLLSDKDAYQ-QFLLSLDQVKVQNNIKDELRRETLQLARDNLEKEPQIMELRN 123
Query: 580 ----LLTTNMETAQENLNQVE 630
+ TT + TAQE LN++E
Sbjct: 124 QCRIIRTTELATAQEKLNELE 144
>At3g10340.1 68416.m01240 phenylalanine ammonia-lyase, putative
similar to phenylalanine ammonia-lyase GB:S48726
[Petroselinum crispum]
Length = 707
Score = 29.1 bits (62), Expect = 2.7
Identities = 18/50 (36%), Positives = 25/50 (50%)
Frame = -1
Query: 293 SALIGDFINSSTLSTNSASGIPEYDLGFEGSTPSPSIFIYCEVYIQFLEN 144
S L+ DF N+ S S P D GF+G+ ++ YC +QFL N
Sbjct: 419 SELVNDFYNNGLPSNLSGGRNPSLDYGFKGA--EIAMASYCS-ELQFLAN 465
>At3g47060.1 68416.m05110 FtsH protease, putative contains
similarity to FtsH protease GI:13183728 from [Medicago
sativa]
Length = 802
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/44 (27%), Positives = 22/44 (50%)
Frame = +1
Query: 196 GVEPSNPKSYSGIPEAEFVDNVDEFMKSPINAEGVDVVLKSLDE 327
G EP ++ +P +EF+ V+ + +GV V+ K D+
Sbjct: 165 GSEPRIQTTFVSVPYSEFLSKVNSNQVQKVEVDGVQVLFKLRDD 208
>At2g29330.1 68415.m03562 tropinone reductase, putative / tropine
dehydrogenase, putative similar to tropinone reductase
SP:P50165 from [Datura stramonium]
Length = 260
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/30 (43%), Positives = 21/30 (70%)
Frame = +1
Query: 550 LEYSLEDAEKLLTTNMETAQENLNQVEHDL 639
+EY ED L++TN+E+A +L+Q+ H L
Sbjct: 104 IEYEAEDFSFLISTNLESAY-HLSQLSHPL 132
>At1g60200.1 68414.m06781 splicing factor PWI domain-containing
protein / RNA recognition motif (RRM)-containing protein
contains Pfam profiles PF01480: PWI domain, PF00076: RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 899
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +1
Query: 370 KRRRLRQQIPDLARTIEVIEKLKEQKEEVETQFLLSDQV 486
+RR+LR++ DLA ++ E++ E K E Q L Q+
Sbjct: 539 RRRQLREKEDDLADRLKEEEEVAEAKRSAEEQNLQQQQL 577
>At1g10950.1 68414.m01257 endomembrane protein 70, putative
Length = 589
Score = 27.9 bits (59), Expect = 6.2
Identities = 8/25 (32%), Positives = 17/25 (68%)
Frame = -3
Query: 255 IYKFSFWYSRIRLRVRGFYSVSFHF 181
+Y +S +Y ++ ++ GF+ SF+F
Sbjct: 529 VYLYSIYYYYVKTKMSGFFQTSFYF 553
>At3g19720.2 68416.m02498 dynamin family protein identical to cDNA
dynamin-like protein (ARC5) GI: 30349145; contains Pfam
profile PF00350: Dynamin family
Length = 741
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +1
Query: 352 EYTLATKRRRLRQQIPDLARTIEVIEKL 435
++++AT RR + Q P+L+RTI V KL
Sbjct: 206 DWSIATTRRIVMQVDPELSRTIVVSTKL 233
>At3g19720.1 68416.m02497 dynamin family protein identical to cDNA
dynamin-like protein (ARC5) GI: 30349145; contains Pfam
profile PF00350: Dynamin family
Length = 777
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +1
Query: 352 EYTLATKRRRLRQQIPDLARTIEVIEKL 435
++++AT RR + Q P+L+RTI V KL
Sbjct: 206 DWSIATTRRIVMQVDPELSRTIVVSTKL 233
>At1g30190.1 68414.m03690 expressed protein ; expression supported
by MPSS
Length = 278
Score = 27.5 bits (58), Expect = 8.2
Identities = 17/57 (29%), Positives = 31/57 (54%)
Frame = +1
Query: 313 KSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIEVIEKLKEQKEEVETQFLLSDQ 483
+S +K R+ ++ T TKR+ ++ D +R + + EK + KEE E LS++
Sbjct: 195 RSKSDKPRRKRLSVDTETTKRKSYGRKKSDCSRWMVIPEKWEYVKEESEEFSKLSNE 251
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,161,173
Number of Sequences: 28952
Number of extensions: 201063
Number of successful extensions: 739
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 737
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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