Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_P20
(652 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g31600.3 68414.m03879 oxidoreductase, 2OG-Fe(II) oxygenase fa... 44 9e-05
At1g31600.2 68414.m03877 oxidoreductase, 2OG-Fe(II) oxygenase fa... 44 9e-05
At1g31600.1 68414.m03878 oxidoreductase, 2OG-Fe(II) oxygenase fa... 44 9e-05
At4g20350.1 68417.m02970 expressed protein hypothetical protein ... 33 0.22
At4g02485.1 68417.m00337 oxidoreductase, 2OG-Fe(II) oxygenase fa... 33 0.22
At1g08140.1 68414.m00896 cation/hydrogen exchanger (CHX6a) Note:... 32 0.29
At1g64540.1 68414.m07316 F-box family protein contains F-box dom... 31 0.66
At1g31360.1 68414.m03838 DNA helicase, putative (RECQl2) nearly ... 31 0.88
At5g40780.2 68418.m04951 lysine and histidine specific transport... 29 2.0
At5g40780.1 68418.m04950 lysine and histidine specific transport... 29 2.0
At1g22590.2 68414.m02821 MADS-box family protein similar to puta... 29 2.7
At2g17970.1 68415.m02084 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 4.7
At5g43040.1 68418.m05254 DC1 domain-containing protein contains ... 27 8.2
At1g24400.1 68414.m03076 lysine and histidine specific transport... 27 8.2
>At1g31600.3 68414.m03879 oxidoreductase, 2OG-Fe(II) oxygenase
family protein contains Pfam profiles PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family, PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 431
Score = 44.0 bits (99), Expect = 9e-05
Identities = 36/129 (27%), Positives = 64/129 (49%), Gaps = 12/129 (9%)
Frame = +3
Query: 294 PTAYYISKFITEAEEQLILSNIYAAPKPKWTQLSNRXLQNWG-----GVPH--NKGMIAE 452
P + + F+T AEEQ +L+ + A W L+ R +Q++G G + K + E
Sbjct: 211 PGLFLLPDFVTVAEEQQLLAAVDAR---HWIGLAKRRVQHYGYEFCYGTRNVDTKKRLGE 267
Query: 453 TIPTWLDLYLERIHKL----NVMCGRRPNHVLVNEYTPGQGIMPHLD-GNLFYPTITTIS 617
+P+++ LERI+ N + + VNEY G G+ PH+D + F I ++S
Sbjct: 268 -LPSFVSPILERIYLFPNFDNGSASLNLDQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLS 326
Query: 618 VGSHIVLKF 644
+ +++F
Sbjct: 327 LAGPCIMEF 335
>At1g31600.2 68414.m03877 oxidoreductase, 2OG-Fe(II) oxygenase
family protein contains Pfam profiles PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family, PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 344
Score = 44.0 bits (99), Expect = 9e-05
Identities = 36/129 (27%), Positives = 64/129 (49%), Gaps = 12/129 (9%)
Frame = +3
Query: 294 PTAYYISKFITEAEEQLILSNIYAAPKPKWTQLSNRXLQNWG-----GVPH--NKGMIAE 452
P + + F+T AEEQ +L+ + A W L+ R +Q++G G + K + E
Sbjct: 124 PGLFLLPDFVTVAEEQQLLAAVDAR---HWIGLAKRRVQHYGYEFCYGTRNVDTKKRLGE 180
Query: 453 TIPTWLDLYLERIHKL----NVMCGRRPNHVLVNEYTPGQGIMPHLD-GNLFYPTITTIS 617
+P+++ LERI+ N + + VNEY G G+ PH+D + F I ++S
Sbjct: 181 -LPSFVSPILERIYLFPNFDNGSASLNLDQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLS 239
Query: 618 VGSHIVLKF 644
+ +++F
Sbjct: 240 LAGPCIMEF 248
>At1g31600.1 68414.m03878 oxidoreductase, 2OG-Fe(II) oxygenase
family protein contains Pfam profiles PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family, PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 431
Score = 44.0 bits (99), Expect = 9e-05
Identities = 36/129 (27%), Positives = 64/129 (49%), Gaps = 12/129 (9%)
Frame = +3
Query: 294 PTAYYISKFITEAEEQLILSNIYAAPKPKWTQLSNRXLQNWG-----GVPH--NKGMIAE 452
P + + F+T AEEQ +L+ + A W L+ R +Q++G G + K + E
Sbjct: 211 PGLFLLPDFVTVAEEQQLLAAVDAR---HWIGLAKRRVQHYGYEFCYGTRNVDTKKRLGE 267
Query: 453 TIPTWLDLYLERIHKL----NVMCGRRPNHVLVNEYTPGQGIMPHLD-GNLFYPTITTIS 617
+P+++ LERI+ N + + VNEY G G+ PH+D + F I ++S
Sbjct: 268 -LPSFVSPILERIYLFPNFDNGSASLNLDQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLS 326
Query: 618 VGSHIVLKF 644
+ +++F
Sbjct: 327 LAGPCIMEF 335
>At4g20350.1 68417.m02970 expressed protein hypothetical protein -
Caenorhabditis elegans,SPTREMBL:Q17527
Length = 139
Score = 32.7 bits (71), Expect = 0.22
Identities = 11/26 (42%), Positives = 18/26 (69%)
Frame = +3
Query: 567 MPHLDGNLFYPTITTISVGSHIVLKF 644
MPH DG ++P + +S+GS +V+ F
Sbjct: 1 MPHQDGPAYFPVVAILSLGSPVVMDF 26
>At4g02485.1 68417.m00337 oxidoreductase, 2OG-Fe(II) oxygenase
family protein contains Pfam domain PF03171, 2OG-Fe(II)
oxygenase superfamily
Length = 226
Score = 32.7 bits (71), Expect = 0.22
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = +3
Query: 525 NHVLVNEYTPGQGIMPHLDGNLFYPTITTISVGSHIVLKF 644
+ ++VN Y PG+GI H+D F I +S+ S V++F
Sbjct: 121 DQLIVNLYQPGEGICAHVDLLRFEDGIAIVSLESPCVMRF 160
>At1g08140.1 68414.m00896 cation/hydrogen exchanger (CHX6a) Note:
CHX6a and CHX6b were originally 1 gene but were split
pased on alignments with other family members; may be a
pseudogene and requires futher investigation; monovalent
cation:proton antiporter family 2 (CPA2) member,
PMID:11500563
Length = 818
Score = 32.3 bits (70), Expect = 0.29
Identities = 14/44 (31%), Positives = 24/44 (54%)
Frame = -1
Query: 553 GVYSFTNTWFGRLPHITFNL*ILSRYRSNQVGIVSAIIPLLCGT 422
G + F WF + + L + RS+ +G ++ IIPL+CG+
Sbjct: 128 GAFGFVLYWFLKGVTMDAELPFRTEKRSSVIGFITVIIPLICGS 171
>At1g64540.1 68414.m07316 F-box family protein contains F-box domain
Pfam:PF00646
Length = 444
Score = 31.1 bits (67), Expect = 0.66
Identities = 11/23 (47%), Positives = 19/23 (82%)
Frame = +3
Query: 558 QGIMPHLDGNLFYPTITTISVGS 626
QG++PH DG +F+P + T+S+G+
Sbjct: 132 QGLVPH-DGTVFFPALKTLSLGA 153
>At1g31360.1 68414.m03838 DNA helicase, putative (RECQl2) nearly
identical to DNA Helicase [Arabidopsis thaliana]
GI:11121445
Length = 705
Score = 30.7 bits (66), Expect = 0.88
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = -1
Query: 547 YSFTNTWFGRLPHITFNL*ILSRYRSNQVGIVSAII 440
+S T W R + FN+ +S+YR+NQ I++AI+
Sbjct: 67 WSETFEWDSRADDVRFNVFGISKYRANQKEIINAIM 102
>At5g40780.2 68418.m04951 lysine and histidine specific transporter,
putative strong similarity to lysine and histidine
specific transporter GI:2576361 from [Arabidopsis
thaliana]; contains Pfam profile PF01490: Transmembrane
amino acid transporter protein
Length = 445
Score = 29.5 bits (63), Expect = 2.0
Identities = 20/72 (27%), Positives = 36/72 (50%)
Frame = -2
Query: 555 LVYIHLPIHGLVVYHT*HLICEFFRDIGLTK*VLFLLSFPYCVGHLPNFVXYDLIAVSI* 376
+VY+ L +H L+C+ + I LT ++ S + + HLPNF + VS+
Sbjct: 131 IVYMVTGGKSLKKFH--ELVCDDCKPIKLTYFIMIFASVHFVLSHLPNF--NSISGVSLA 186
Query: 375 ASVLHICLTVSA 340
A+V+ + + A
Sbjct: 187 AAVMSLSYSTIA 198
>At5g40780.1 68418.m04950 lysine and histidine specific transporter,
putative strong similarity to lysine and histidine
specific transporter GI:2576361 from [Arabidopsis
thaliana]; contains Pfam profile PF01490: Transmembrane
amino acid transporter protein
Length = 446
Score = 29.5 bits (63), Expect = 2.0
Identities = 20/72 (27%), Positives = 36/72 (50%)
Frame = -2
Query: 555 LVYIHLPIHGLVVYHT*HLICEFFRDIGLTK*VLFLLSFPYCVGHLPNFVXYDLIAVSI* 376
+VY+ L +H L+C+ + I LT ++ S + + HLPNF + VS+
Sbjct: 132 IVYMVTGGKSLKKFH--ELVCDDCKPIKLTYFIMIFASVHFVLSHLPNF--NSISGVSLA 187
Query: 375 ASVLHICLTVSA 340
A+V+ + + A
Sbjct: 188 AAVMSLSYSTIA 199
>At1g22590.2 68414.m02821 MADS-box family protein similar to
putative DNA-binding protein GI:6714399 from
[Arabidopsis thaliana]; MADS-box protein AGL87
Length = 163
Score = 29.1 bits (62), Expect = 2.7
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = +3
Query: 471 DLYLERIHKLNVMCGRRPNHVLVNEYTPGQGIMPHLD 581
D L++I++L V+CG ++ +EY G + P+L+
Sbjct: 26 DGLLKKIYELTVLCGLPACAIIYSEYKDGPELWPNLN 62
>At2g17970.1 68415.m02084 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to At2g17960, At4g36090; contains
PF03171 2OG-Fe(II) oxygenase superfamily domain
Length = 507
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/41 (36%), Positives = 20/41 (48%)
Frame = +3
Query: 522 PNHVLVNEYTPGQGIMPHLDGNLFYPTITTISVGSHIVLKF 644
P+ +VN Y G I PH+D + F TIS S + F
Sbjct: 318 PDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILF 358
>At5g43040.1 68418.m05254 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 551
Score = 27.5 bits (58), Expect = 8.2
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = -3
Query: 275 VIFNICYICHHYSLVFICTCS 213
+ FN CYIC H + TC+
Sbjct: 286 IFFNACYICTHCDFILHETCA 306
>At1g24400.1 68414.m03076 lysine and histidine specific transporter,
putative similar to lysine and histidine specific
transporter GI:2576361 from [Arabidopsis thaliana];
contains Pfam profile PF01490: Transmembrane amino acid
transporter protein
Length = 441
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/40 (27%), Positives = 22/40 (55%)
Frame = -2
Query: 501 LICEFFRDIGLTK*VLFLLSFPYCVGHLPNFVXYDLIAVS 382
L+C ++I T ++ S + + HLPNF +I+++
Sbjct: 143 LVCPDCKEIRTTFWIMIFASVHFVISHLPNFNSISIISLA 182
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,710,089
Number of Sequences: 28952
Number of extensions: 272205
Number of successful extensions: 620
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 606
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 617
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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