Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_P07
(651 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 37 0.013
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 37 0.013
At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17... 35 0.041
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 34 0.094
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 31 0.50
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 31 0.66
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 29 3.5
At5g04290.1 68418.m00422 KOW domain-containing transcription fac... 28 6.2
At3g49540.1 68416.m05414 expressed protein 28 6.2
At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 27 8.2
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 36.7 bits (81), Expect = 0.013
Identities = 26/104 (25%), Positives = 53/104 (50%), Gaps = 8/104 (7%)
Frame = +3
Query: 285 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEGKHXXXKDQHG----YISR---QFTR 440
K + ++ LD+ +E+ ++ + G + V G+ +++ G + R +F R
Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135
Query: 441 RYALPEGCTAESVESRLSSDGVLSVIAPXKVPPAVEGERKIPIA 572
++ LP+ ESV+++L +GVL++ P V+G R + IA
Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTINLTKLSPEKVKGPRVVNIA 178
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 36.7 bits (81), Expect = 0.013
Identities = 28/93 (30%), Positives = 45/93 (48%), Gaps = 8/93 (8%)
Frame = +3
Query: 318 DVQHFAPEEISVKTADGYIV-VEGKHXXXKDQHG-------YISRQFTRRYALPEGCTAE 473
D+ EE+ V+ DG I+ + G+ ++ S +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 474 SVESRLSSDGVLSVIAPXKVPPAVEGERKIPIA 572
+++ + +GVLSV P KVP + I I+
Sbjct: 126 EIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156
>At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17.6A
(HSP17.7-CII) identical to heat shock protein 17.6A
GI:3256075 from [Arabidopsis thaliana]
Length = 156
Score = 35.1 bits (77), Expect = 0.041
Identities = 34/126 (26%), Positives = 55/126 (43%), Gaps = 9/126 (7%)
Frame = +3
Query: 219 SREYYRPWRHLAAAARDLGSSIKSDKDKFQVNLDVQHFAPEEISVKTA-DGYIVVEGKHX 395
SR Y R + +AA D + D + +D+ +EI V+ + +VV GK
Sbjct: 33 SRAYMRDAKAMAATPAD----VIEHPDAYVFAVDMPGIKGDEIQVQIENENVLVVSGKRQ 88
Query: 396 XX-KDQHGY----ISRQ---FTRRYALPEGCTAESVESRLSSDGVLSVIAPXKVPPAVEG 551
K+ G + R+ F R++ LP+ E + S +DGVL V P PP +
Sbjct: 89 RDNKENEGVKFVRMERRMGKFMRKFQLPDNADLEKI-SAACNDGVLKVTIPKLPPPEPKK 147
Query: 552 ERKIPI 569
+ I +
Sbjct: 148 PKTIQV 153
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 33.9 bits (74), Expect = 0.094
Identities = 24/77 (31%), Positives = 37/77 (48%), Gaps = 8/77 (10%)
Frame = +3
Query: 318 DVQHFAPEEISVKTADGYIV-VEGKHXXXKDQHGYI-------SRQFTRRYALPEGCTAE 473
DV EE+ V+ DG I+ + G+ ++ S +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 474 SVESRLSSDGVLSVIAP 524
V++ + +GVLSV P
Sbjct: 125 EVKASM-ENGVLSVTVP 140
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 31.5 bits (68), Expect = 0.50
Identities = 23/77 (29%), Positives = 39/77 (50%), Gaps = 8/77 (10%)
Frame = +3
Query: 318 DVQHFAPEEISVKTA-DGYIVVEG-KHXXXKDQHGYISR------QFTRRYALPEGCTAE 473
D+ EE+ V+ D + + G +H +D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 474 SVESRLSSDGVLSVIAP 524
V++ + +GVL+V P
Sbjct: 122 QVKAAM-ENGVLTVTVP 137
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 31.1 bits (67), Expect = 0.66
Identities = 18/50 (36%), Positives = 28/50 (56%)
Frame = +3
Query: 423 SRQFTRRYALPEGCTAESVESRLSSDGVLSVIAPXKVPPAVEGERKIPIA 572
S +F RR+ LPE E V++ + +GVL+V+ P K P + I I+
Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPEKKPQVKSIDIS 158
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 28.7 bits (61), Expect = 3.5
Identities = 21/77 (27%), Positives = 39/77 (50%), Gaps = 8/77 (10%)
Frame = +3
Query: 318 DVQHFAPEEISVKTADGYIV-VEGKHXXXKDQ-----HGY--ISRQFTRRYALPEGCTAE 473
D+ EE+ V+ D ++ + G+ K++ H S QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 474 SVESRLSSDGVLSVIAP 524
V++ + +GVL+V P
Sbjct: 124 QVKASM-ENGVLTVTVP 139
>At5g04290.1 68418.m00422 KOW domain-containing transcription factor
family protein
Length = 1493
Score = 27.9 bits (59), Expect = 6.2
Identities = 16/44 (36%), Positives = 20/44 (45%)
Frame = +2
Query: 260 GSRPWLQHQK**G*VPSKPGRPAFRPGRNLC*NGGRLHRG*RQT 391
GS+PW +H + G FR GRN GGR G R +
Sbjct: 1321 GSKPWNEHSGGGRGFGERRGGGGFRGGRNQSGRGGRSFDGGRSS 1364
>At3g49540.1 68416.m05414 expressed protein
Length = 166
Score = 27.9 bits (59), Expect = 6.2
Identities = 17/49 (34%), Positives = 28/49 (57%)
Frame = +3
Query: 456 EGCTAESVESRLSSDGVLSVIAPXKVPPAVEGERKIPIAQTGPVRKEVK 602
E E+ E+++ + ++V AP KV AVE E+K A+ PV+ E +
Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKK---AEAEPVKAEAE 142
>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 478
Score = 27.5 bits (58), Expect = 8.2
Identities = 23/59 (38%), Positives = 25/59 (42%), Gaps = 2/59 (3%)
Frame = -1
Query: 483 TPQIQPYSPPAKRSDE*IDAICNHADLXXPXVCLQPR--CSRPPF*QRFLPGRNAGRPG 313
T I+ YS A I N A L P PR S PPF LPG+NAG G
Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,198,896
Number of Sequences: 28952
Number of extensions: 312557
Number of successful extensions: 766
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 766
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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