Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_O19
(352 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein L27|Schizosacch... 69 3e-13
SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein L27|Schizosac... 69 3e-13
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 30 0.091
SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces pomb... 27 0.64
SPCC622.18 |rpl6||60S ribosomal protein L6|Schizosaccharomyces p... 26 1.5
SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||... 26 2.0
SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr... 26 2.0
SPCC11E10.06c |||RNA polymerase II elongator complex subunit Elp... 25 2.6
SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyce... 25 3.4
SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces pombe... 25 4.5
SPBC1604.04 |||thiamine pyrophosphate transporter|Schizosaccharo... 25 4.5
SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr 1|||M... 24 6.0
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 24 6.0
SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr... 24 7.9
SPAC23H4.17c |srb10|prk1, cdk8|cyclin-dependent protein kinase S... 24 7.9
>SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein
L27|Schizosaccharomyces pombe|chr 3|||Manual
Length = 136
Score = 68.5 bits (160), Expect = 3e-13
Identities = 28/58 (48%), Positives = 40/58 (68%)
Frame = +1
Query: 1 MGXIMKPGKVVLVLSGRYAGRKAIVXXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDG 174
M I+KPGKV LV GR+AG+K ++ N D+G+ P+GHA VAG++RYP + + G
Sbjct: 1 MVKILKPGKVALVTRGRFAGKKVVILQNVDQGSKSHPFGHAVVAGVERYPLKVTKSMG 58
Score = 63.7 bits (148), Expect = 8e-12
Identities = 30/70 (42%), Positives = 43/70 (61%), Gaps = 2/70 (2%)
Frame = +2
Query: 149 PGKVHKRMGXNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEK--FSAKDLKDPAKRKK 322
P KV K MG +I KRS++KPF+KV+NYNHLMPTRY ++ K + +P++R
Sbjct: 50 PLKVTKSMGAKRIAKRSRVKPFIKVINYNHLMPTRYALELDNLKGLVTPTTFSEPSQRSA 109
Query: 323 LRFNTRVXFE 352
+ + FE
Sbjct: 110 AKKTVKNTFE 119
>SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein
L27|Schizosaccharomyces pombe|chr 2|||Manual
Length = 136
Score = 68.5 bits (160), Expect = 3e-13
Identities = 33/70 (47%), Positives = 44/70 (62%), Gaps = 2/70 (2%)
Frame = +2
Query: 149 PGKVHKRMGXNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEK--FSAKDLKDPAKRKK 322
P KV K MG +I +RS++KPF+KVVNYNHLMPTRY ++ K +A K+P +R
Sbjct: 50 PLKVTKSMGAKRIARRSRVKPFIKVVNYNHLMPTRYALELDNLKGLITADTFKEPTQRSA 109
Query: 323 LRFNTRVXFE 352
R + FE
Sbjct: 110 ARKTVKKTFE 119
Score = 64.9 bits (151), Expect = 3e-12
Identities = 26/58 (44%), Positives = 39/58 (67%)
Frame = +1
Query: 1 MGXIMKPGKVVLVLSGRYAGRKAIVXXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDG 174
M I+KPGKV L+ GR+AG+K ++ D+G+ P+GHA VAG++RYP + + G
Sbjct: 1 MVKILKPGKVALITRGRFAGKKVVILQAIDQGSKSHPFGHAVVAGVERYPLKVTKSMG 58
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 30.3 bits (65), Expect = 0.091
Identities = 17/52 (32%), Positives = 21/52 (40%)
Frame = -1
Query: 289 CAEFFKAEVNCITCWHQVIIVYNLHERLYLGPLVDFIXSHPLVHFAGGTCRY 134
CA+F E N + WH YNL R Y + D + P G C Y
Sbjct: 772 CAKFSLKETNKTSFWHS---SYNLFNRQYHVDMPDNVSDLPQTRLCYGACVY 820
>SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 225
Score = 27.5 bits (58), Expect = 0.64
Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%)
Frame = +2
Query: 173 GXNKIHKRSKIKPFVKVVNYNHLMPTRYTVDF-SFEKFSAKDLKDP 307
G + I R K P K+VNYNH + + +F K D+ DP
Sbjct: 28 GNHWIFARFKFYPLNKIVNYNHFHSSSCQSEAKNFYKQFEGDISDP 73
>SPCC622.18 |rpl6||60S ribosomal protein L6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 195
Score = 26.2 bits (55), Expect = 1.5
Identities = 8/27 (29%), Positives = 18/27 (66%)
Frame = +1
Query: 13 MKPGKVVLVLSGRYAGRKAIVXXNYDE 93
+ PG V ++L+GR+ G++ +V ++
Sbjct: 52 LAPGTVCILLAGRFRGKRVVVLSQLED 78
>SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1260
Score = 25.8 bits (54), Expect = 2.0
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 155 KVHKRMGXNKIHKRSKIKPFVKVV 226
K+ KR+G I SK+ P VK+V
Sbjct: 205 KIAKRIGFTDISLSSKLTPMVKIV 228
>SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 872
Score = 25.8 bits (54), Expect = 2.0
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = -1
Query: 304 VFQVFCAEFFKAEVNCITCWHQVIIVYNLHERLY 203
V+QVFC F A+ I +Q++ V NL E Y
Sbjct: 315 VYQVFCTNFPNAQQLIIDNQNQLLKVANLLEPSY 348
>SPCC11E10.06c |||RNA polymerase II elongator complex subunit Elp4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 361
Score = 25.4 bits (53), Expect = 2.6
Identities = 18/61 (29%), Positives = 25/61 (40%), Gaps = 3/61 (4%)
Frame = +2
Query: 152 GKVHKRMGXNKIHKRSKIKPFVKVVNYNHLMPTRYTVD---FSFEKFSAKDLKDPAKRKK 322
G V + +G +IHK PF N N +TV F+ E + L D K K
Sbjct: 286 GLVTQPLGLFRIHKLPLALPFTNHANSNEAGDLSFTVSKRRFTIEPWVLPPLDDEQKDTK 345
Query: 323 L 325
+
Sbjct: 346 I 346
>SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 134
Score = 25.0 bits (52), Expect = 3.4
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 22 GKVVLVLSGRYAGRKAIV 75
G+VVLV G Y G+ A++
Sbjct: 11 GRVVLVTKGEYTGKLAVI 28
>SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 504
Score = 24.6 bits (51), Expect = 4.5
Identities = 14/46 (30%), Positives = 19/46 (41%)
Frame = +2
Query: 203 IKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRKKLRFNTR 340
+ PF K Y H + T + S KF + K K R +TR
Sbjct: 39 VSPFFKKERYKHHLETNVSSHQSATKFIEPEPSPTKKTKLTRRDTR 84
>SPBC1604.04 |||thiamine pyrophosphate
transporter|Schizosaccharomyces pombe|chr 2|||Manual
Length = 314
Score = 24.6 bits (51), Expect = 4.5
Identities = 11/27 (40%), Positives = 13/27 (48%)
Frame = -3
Query: 260 LYNVLASSDYSLQPSRKALSWTSCGFY 180
LY L+ S + P R W SC FY
Sbjct: 246 LYRGLSVSMLKVAPGRYECLWLSCHFY 272
>SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 757
Score = 24.2 bits (50), Expect = 6.0
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = -3
Query: 233 YSLQPSRKALSWTSCGFYXFPSSCALC 153
YS+ PS +L W + + PS LC
Sbjct: 16 YSILPSYSSLQWITNAWCSLPSIYILC 42
>SPBC354.13 |rga6||GTPase activating protein
Rga6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 733
Score = 24.2 bits (50), Expect = 6.0
Identities = 8/25 (32%), Positives = 16/25 (64%)
Frame = -1
Query: 286 AEFFKAEVNCITCWHQVIIVYNLHE 212
A FF + +C++ + ++ YN+HE
Sbjct: 69 APFFTKQNSCLSRFRELAQTYNIHE 93
>SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 454
Score = 23.8 bits (49), Expect = 7.9
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = -2
Query: 159 TLPGVPVDTSDESMPVRLVGGTFV 88
TLP VPV D+++ V+ +G V
Sbjct: 132 TLPTVPVGPEDKAVVVQKIGSPLV 155
>SPAC23H4.17c |srb10|prk1, cdk8|cyclin-dependent protein kinase
Srb10 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 352
Score = 23.8 bits (49), Expect = 7.9
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 134 VSTGTPGKVHKRMGXNKIHKR 196
+S+GT GKV+K + N KR
Sbjct: 11 ISSGTYGKVYKAVSSNSNDKR 31
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,357,663
Number of Sequences: 5004
Number of extensions: 25391
Number of successful extensions: 78
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 74
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 77
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 106195544
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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