Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_O19
(352 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A) 70 4e-13
At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) simi... 68 2e-12
At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C) 67 3e-12
At1g12440.2 68414.m01438 zinc finger (AN1-like) family protein c... 28 1.5
At1g12440.1 68414.m01437 zinc finger (AN1-like) family protein c... 28 1.5
At4g35120.1 68417.m04992 kelch repeat-containing F-box family pr... 27 3.5
At1g72440.1 68414.m08377 CCAAT-box-binding transcription factor-... 27 3.5
At3g23320.1 68416.m02941 hypothetical protein 27 4.6
At1g48175.1 68414.m05377 deaminase-related similar to tRNA-speci... 27 4.6
At5g39550.1 68418.m04791 zinc finger (C3HC4-type RING finger) fa... 26 6.1
At1g66050.1 68414.m07497 zinc finger (C3HC4-type RING finger) fa... 26 6.1
At1g66040.1 68414.m07495 zinc finger (C3HC4-type RING finger) fa... 26 6.1
At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) simila... 26 6.1
At2g19650.1 68415.m02296 DC1 domain-containing protein contains ... 26 8.1
At1g70140.1 68414.m08071 formin homology 2 domain-containing pro... 26 8.1
>At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A)
Length = 135
Score = 70.1 bits (164), Expect = 4e-13
Identities = 28/67 (41%), Positives = 45/67 (67%)
Frame = +1
Query: 13 MKPGKVVLVLSGRYAGRKAIVXXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDGKX*NPQ 192
MKPGK V++L GRY G+KA++ ++D+GT +K YGH VAG+ +YP + ++D +
Sbjct: 5 MKPGKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAKKTAK 64
Query: 193 EVQDKAF 213
+ + K F
Sbjct: 65 KSRVKCF 71
Score = 53.6 bits (123), Expect = 4e-08
Identities = 30/71 (42%), Positives = 41/71 (57%), Gaps = 7/71 (9%)
Frame = +2
Query: 149 PGKVHKRMGXNKIHKRSKIKPFVKVVNYNHLMPTRYTVDF------SFEKFSAKDLKDPA 310
P KV ++ K K+S++K F KV+NY H+MPTRYT+D S + S+KD K A
Sbjct: 50 PSKVIRKDSAKKTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTA 109
Query: 311 -KRKKLRFNTR 340
K K +F R
Sbjct: 110 LKEAKAKFEER 120
>At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) similar
to 60S RIBOSOMAL PROTEIN L27 GB:P41101 from [Solanum
tuberosum]
Length = 135
Score = 67.7 bits (158), Expect = 2e-12
Identities = 28/71 (39%), Positives = 46/71 (64%)
Frame = +1
Query: 1 MGXIMKPGKVVLVLSGRYAGRKAIVXXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDGKX 180
M +K K V++L GRYAG+KA++ ++D+GTSD+ YGH VAG+ +YP + ++D
Sbjct: 1 MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 181 *NPQEVQDKAF 213
++ + K F
Sbjct: 61 KTAKKSRVKCF 71
Score = 54.4 bits (125), Expect = 2e-08
Identities = 25/57 (43%), Positives = 37/57 (64%), Gaps = 1/57 (1%)
Frame = +2
Query: 149 PGKVHKRMGXNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD-LKDPAKR 316
P KV ++ K K+S++K F+K+VNY HLMPTRYT+D ++ + D LK K+
Sbjct: 50 PSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKK 106
>At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C)
Length = 135
Score = 67.3 bits (157), Expect = 3e-12
Identities = 27/71 (38%), Positives = 45/71 (63%)
Frame = +1
Query: 1 MGXIMKPGKVVLVLSGRYAGRKAIVXXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDGKX 180
M +K K V++L GRYAG+KA++ ++D+G D+PYGH VAG+ +YP + ++D
Sbjct: 1 MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 181 *NPQEVQDKAF 213
++ + K F
Sbjct: 61 KTAKKSRVKCF 71
Score = 54.0 bits (124), Expect = 3e-08
Identities = 23/57 (40%), Positives = 35/57 (61%)
Frame = +2
Query: 149 PGKVHKRMGXNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRK 319
P KV ++ K K+S++K F+K+VNY HLMPTRYT+D ++ + D +K
Sbjct: 50 PSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKK 106
>At1g12440.2 68414.m01438 zinc finger (AN1-like) family protein
contains Pfam domains, PF01428: AN1-like Zinc finger and
PF01754: A20-like zinc finger
Length = 168
Score = 28.3 bits (60), Expect = 1.5
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -3
Query: 230 SLQPSRKALSWTSCGFYXFPSSCALC-RGYLSIPATK 123
S PS L CGF+ PS+ LC + Y I AT+
Sbjct: 10 SFSPSEPKLCVKGCGFFGSPSNMNLCSKCYRDIRATE 46
>At1g12440.1 68414.m01437 zinc finger (AN1-like) family protein
contains Pfam domains, PF01428: AN1-like Zinc finger and
PF01754: A20-like zinc finger
Length = 168
Score = 28.3 bits (60), Expect = 1.5
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -3
Query: 230 SLQPSRKALSWTSCGFYXFPSSCALC-RGYLSIPATK 123
S PS L CGF+ PS+ LC + Y I AT+
Sbjct: 10 SFSPSEPKLCVKGCGFFGSPSNMNLCSKCYRDIRATE 46
>At4g35120.1 68417.m04992 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 389
Score = 27.1 bits (57), Expect = 3.5
Identities = 19/67 (28%), Positives = 31/67 (46%), Gaps = 3/67 (4%)
Frame = -3
Query: 212 KALSWTSCGFYXFPSSCALCRGYLSIPATKACPYGLSEVPSS*XLXTIAL---RPAYRPL 42
++LS S FY SS + I AT+ CPY +P T+A + + P+
Sbjct: 48 RSLSLVSKSFYSLLSSTEIYAARSHIGATEPCPYVCLWLPKKHRWFTLAEIEGKLSLEPV 107
Query: 41 KTSTTLP 21
+ S++ P
Sbjct: 108 RLSSSYP 114
>At1g72440.1 68414.m08377 CCAAT-box-binding transcription
factor-related similar to CCAAT-box-binding transcription
factor (CCAAT-binding factor) (CBF) (Swiss-Prot:Q03701)
[Homo sapiens], GB:P53569 [Mus musculus]
Length = 1056
Score = 27.1 bits (57), Expect = 3.5
Identities = 19/62 (30%), Positives = 26/62 (41%)
Frame = +2
Query: 137 STGTPGKVHKRMGXNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKR 316
S G G+ K+ K KR + PF + Y HL+ D K K +P K+
Sbjct: 994 SDGDDGRSKKK----KKEKRKRKSPFASLEEYKHLIDQDEKED---SKTKRKATSEPTKK 1046
Query: 317 KK 322
KK
Sbjct: 1047 KK 1048
>At3g23320.1 68416.m02941 hypothetical protein
Length = 191
Score = 26.6 bits (56), Expect = 4.6
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +3
Query: 12 NEAG*SSAGLKWPVRGSQG 68
N AG +GL+W +R SQG
Sbjct: 46 NHAGRQDSGLRWIIRNSQG 64
>At1g48175.1 68414.m05377 deaminase-related similar to tRNA-specific
adenosine deaminase 2 (EC 3.5.4.-) (tRNA-specific
adenosine-34 deaminase subunit TAD2) (Swiss-Prot:P47058)
[Saccharomyces cerevisiae]
Length = 122
Score = 26.6 bits (56), Expect = 4.6
Identities = 13/40 (32%), Positives = 17/40 (42%)
Frame = -3
Query: 227 LQPSRKALSWTSCGFYXFPSSCALCRGYLSIPATKACPYG 108
L PS+ A ++ C Y C +C LS K YG
Sbjct: 16 LSPSQVAEKFSKCVLYVTCEPCIMCASALSFLGIKEVYYG 55
>At5g39550.1 68418.m04791 zinc finger (C3HC4-type RING finger)
family protein contains zinc finger, C3HC4 type (RING
finger), signature, PROSITE:PS00518
Length = 617
Score = 26.2 bits (55), Expect = 6.1
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -3
Query: 167 SCALCRGYLSIPATKACPY 111
SC +CR LS+P T C +
Sbjct: 494 SCQICREVLSLPVTTPCAH 512
>At1g66050.1 68414.m07497 zinc finger (C3HC4-type RING finger)
family protein contains zinc finger, C3HC4 type (RING
finger), signature, PROSITE:PS00518
Length = 623
Score = 26.2 bits (55), Expect = 6.1
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -3
Query: 167 SCALCRGYLSIPATKACPY 111
SC +CR LS+P T C +
Sbjct: 497 SCQICRKVLSLPVTTPCAH 515
>At1g66040.1 68414.m07495 zinc finger (C3HC4-type RING finger)
family protein contains zinc finger, C3HC4 type (RING
finger), signature, PROSITE:PS00518
Length = 622
Score = 26.2 bits (55), Expect = 6.1
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -3
Query: 167 SCALCRGYLSIPATKACPY 111
SC +CR LS+P T C +
Sbjct: 497 SCQICRKVLSLPVTTPCAH 515
>At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) similar
to 60S ribosomal protein L6 GI:7208784 from [Cicer
arietinum]
Length = 233
Score = 26.2 bits (55), Expect = 6.1
Identities = 8/26 (30%), Positives = 16/26 (61%)
Frame = +1
Query: 19 PGKVVLVLSGRYAGRKAIVXXNYDEG 96
PG V+++L+GR+ G++ + G
Sbjct: 91 PGTVLIILAGRFKGKRVVFLKQLSSG 116
>At2g19650.1 68415.m02296 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 682
Score = 25.8 bits (54), Expect = 8.1
Identities = 12/43 (27%), Positives = 18/43 (41%)
Frame = -1
Query: 292 FCAEFFKAEVNCITCWHQVIIVYNLHERLYLGPLVDFIXSHPL 164
FC EFF C C Q+ ++ L + ++ HPL
Sbjct: 114 FCGEFFSVGYRCNKCDFQLDLICARRPALLVLAENSYLHEHPL 156
>At1g70140.1 68414.m08071 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 760
Score = 25.8 bits (54), Expect = 8.1
Identities = 14/38 (36%), Positives = 18/38 (47%)
Frame = +2
Query: 98 PPTSRTGMLSSLVSTGTPGKVHKRMGXNKIHKRSKIKP 211
PP + G LSS S P V G + K+ K+KP
Sbjct: 272 PPVKKVGALSSSASKPPPAPVRGASG-GETSKQVKLKP 308
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,125,106
Number of Sequences: 28952
Number of extensions: 133009
Number of successful extensions: 352
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 349
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 352
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 439384704
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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