Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_O06
(652 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 201 6e-53
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 198 4e-52
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 32 0.083
SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 28 0.20
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 26 5.4
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 7.2
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 25 7.2
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 25 9.5
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 201 bits (491), Expect = 6e-53
Identities = 100/178 (56%), Positives = 127/178 (71%), Gaps = 2/178 (1%)
Frame = +2
Query: 125 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYXNVVXARRSSW 304
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAY NV+ ARR+SW
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62
Query: 305 RVISSIEQK--TXGSXRKQQMAKEYXVKVQKELREICYDVLGLLDKHLIPKASNPESKVF 478
R++SSIEQK + G+ + ++ KEY K+++EL IC D+L +L+KHLIP A++ ESKVF
Sbjct: 63 RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVF 122
Query: 479 YLKMKGDYYRYLAXVATGETXHSVVXDSQKAYQDAFEISKAKMQPTHPIXLGLALNFS 652
Y KMKGDYYRYLA A GE S + Y+ A EI+ A++ PTHPI LGLALNFS
Sbjct: 123 YYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFS 180
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 198 bits (484), Expect = 4e-52
Identities = 100/179 (55%), Positives = 128/179 (71%), Gaps = 2/179 (1%)
Frame = +2
Query: 122 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYXNVVXARRSS 301
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAY N++ ARR+S
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60
Query: 302 WRVISSIEQK--TXGSXRKQQMAKEYXVKVQKELREICYDVLGLLDKHLIPKASNPESKV 475
WR+ISSIEQK + G+ R+ + KEY K++ EL +IC+DVL +L+KHLIP A+ ESKV
Sbjct: 61 WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKV 120
Query: 476 FYLKMKGDYYRYLAXVATGETXHSVVXDSQKAYQDAFEISKAKMQPTHPIXLGLALNFS 652
FY KMKGDYYRYLA GE S +AY+ A +I+ A++ PT P+ LGLALNFS
Sbjct: 121 FYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFS 179
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 31.9 bits (69), Expect = 0.083
Identities = 23/104 (22%), Positives = 43/104 (41%), Gaps = 2/104 (1%)
Frame = +2
Query: 104 PLPSSTMSVDKE--ELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYXN 277
P+ S TMS E + R + + Y+ MA + E E ++ + LLS Y N
Sbjct: 2997 PIMSITMSDSSAYGEELMRERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDN 3056
Query: 278 VVXARRSSWRVISSIEQKTXGSXRKQQMAKEYXVKVQKELREIC 409
+R+ + +E+ + EY +++ L++ C
Sbjct: 3057 EAYQAELFYRLSNCVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100
>SPAC16A10.08c |mug74|SPAC589.01c|sequence
orphan|Schizosaccharomyces pombe|chr 1|||Manual
Length = 285
Score = 28.3 bits (60), Expect(2) = 0.20
Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 5/61 (8%)
Frame = +3
Query: 18 AQEKNSILC-----LNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNWPNKLSD 182
+ ++NSILC L+L+V + ++ S+ + F +PR K N WPN+ S
Sbjct: 40 SDKENSILCKQLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETSH 99
Query: 183 M 185
+
Sbjct: 100 L 100
Score = 21.0 bits (42), Expect(2) = 0.20
Identities = 11/36 (30%), Positives = 15/36 (41%)
Frame = +3
Query: 300 HGVSFPQLNRKPXVXKENSKWQKNIXLKYKRSSEKS 407
H L P + KEN+K + Y +EKS
Sbjct: 109 HSNGLDALAMTPYIVKENNKTHLSPRGNYPSEAEKS 144
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 25.8 bits (54), Expect = 5.4
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +2
Query: 326 QKTXGSXRKQQMAKEYXVKVQKELREICYDVLGLLDKHLIPK 451
+K + KEY K Q++LR+ D+ LL KH I +
Sbjct: 328 EKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 7.2
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +3
Query: 69 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 191
FSP +K + +L LFH + PS+++ V N + SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 25.4 bits (53), Expect = 7.2
Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 3/49 (6%)
Frame = +2
Query: 425 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAXVATGETXHSVVXDS 562
+++K IP++ E+K Y + GD+ +L A E H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +3
Query: 84 KGISELVLFHRPRCPSTRKNWCNVPNW 164
+G+ + L RC +T CNVP W
Sbjct: 694 EGVRKSALSSLWRCATTYYKVCNVPQW 720
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,563,578
Number of Sequences: 5004
Number of extensions: 48085
Number of successful extensions: 137
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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