BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_L24
(650 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q1AG34 Cluster: Ded1-like DEAD-box RNA helicase; n=1; C... 55 1e-06
UniRef50_Q17CR5 Cluster: DEAD box ATP-dependent RNA helicase; n=... 50 6e-05
UniRef50_UPI0000E4A09E Cluster: PREDICTED: hypothetical protein;... 48 3e-04
UniRef50_Q7QDB7 Cluster: ENSANGP00000017541; n=1; Anopheles gamb... 46 0.001
UniRef50_Q9VHP0 Cluster: ATP-dependent RNA helicase bel; n=4; Pr... 44 0.002
UniRef50_O00571 Cluster: ATP-dependent RNA helicase DDX3X; n=74;... 44 0.004
UniRef50_Q1DGU8 Cluster: Catrin, putative; n=2; Culicidae|Rep: C... 35 1.9
UniRef50_Q2QNM2 Cluster: Expressed protein; n=4; Magnoliophyta|R... 34 3.4
UniRef50_A1RQW7 Cluster: Putative uncharacterized protein; n=2; ... 34 3.4
>UniRef50_Q1AG34 Cluster: Ded1-like DEAD-box RNA helicase; n=1;
Chironomus tentans|Rep: Ded1-like DEAD-box RNA helicase
- Chironomus tentans (Midge)
Length = 776
Score = 55.2 bits (127), Expect = 1e-06
Identities = 25/41 (60%), Positives = 33/41 (80%)
Frame = +2
Query: 131 MSNVTNQNGTGLEQQLAGLDLQPQAPKSTGRYIPPHLRRQL 253
M+N N+NGTGLEQQ+AGLDL + KS+ +YIPPHLR ++
Sbjct: 1 MNNYANENGTGLEQQVAGLDLNER--KSSTKYIPPHLRGEI 39
>UniRef50_Q17CR5 Cluster: DEAD box ATP-dependent RNA helicase; n=2;
Aedes aegypti|Rep: DEAD box ATP-dependent RNA helicase -
Aedes aegypti (Yellowfever mosquito)
Length = 625
Score = 49.6 bits (113), Expect = 6e-05
Identities = 29/63 (46%), Positives = 32/63 (50%), Gaps = 8/63 (12%)
Frame = +2
Query: 131 MSNVTNQNGTGLEQQLAGLDLQP--------QAPKSTGRYIPPHLRRQLQATSDQGEESK 286
MSN NQNGTGLEQQ AGLDLQ S+ RY+PP LR G E+
Sbjct: 1 MSNAINQNGTGLEQQFAGLDLQQSQCVQDSGNLKSSSARYVPPQLRSGRGGGGGGGPEND 60
Query: 287 RSS 295
S
Sbjct: 61 NQS 63
>UniRef50_UPI0000E4A09E Cluster: PREDICTED: hypothetical protein;
n=2; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 146
Score = 47.6 bits (108), Expect = 3e-04
Identities = 23/38 (60%), Positives = 26/38 (68%)
Frame = +2
Query: 131 MSNVTNQNGTGLEQQLAGLDLQPQAPKSTGRYIPPHLR 244
MSN TNQNG L+QQ A +DL P A K Y+PPHLR
Sbjct: 1 MSNGTNQNGPSLDQQFAAMDLMPGAKK----YVPPHLR 34
>UniRef50_Q7QDB7 Cluster: ENSANGP00000017541; n=1; Anopheles gambiae
str. PEST|Rep: ENSANGP00000017541 - Anopheles gambiae
str. PEST
Length = 771
Score = 45.6 bits (103), Expect = 0.001
Identities = 30/62 (48%), Positives = 34/62 (54%), Gaps = 16/62 (25%)
Frame = +2
Query: 131 MSNVTNQNGTGLEQQLAGLDLQ-------------PQA--PK-STGRYIPPHLRRQLQAT 262
MSN NQNGTGLEQQ AGLDLQ P++ PK GRY+PP LR +
Sbjct: 1 MSNAINQNGTGLEQQFAGLDLQQKQQQLGGGGGSNPESGNPKHPAGRYVPPQLRECADSG 60
Query: 263 SD 268
D
Sbjct: 61 GD 62
>UniRef50_Q9VHP0 Cluster: ATP-dependent RNA helicase bel; n=4;
Protostomia|Rep: ATP-dependent RNA helicase bel -
Drosophila melanogaster (Fruit fly)
Length = 798
Score = 44.4 bits (100), Expect = 0.002
Identities = 27/52 (51%), Positives = 31/52 (59%), Gaps = 14/52 (26%)
Frame = +2
Query: 131 MSNVTNQNGTGLEQQLAGLDLQ--------PQAPKST------GRYIPPHLR 244
MSN NQNGTGLEQQ+AGLDL P K++ G Y+PPHLR
Sbjct: 1 MSNAINQNGTGLEQQVAGLDLNGGSADYSGPITSKTSTNSVTGGVYVPPHLR 52
>UniRef50_O00571 Cluster: ATP-dependent RNA helicase DDX3X; n=74;
Metazoa|Rep: ATP-dependent RNA helicase DDX3X - Homo
sapiens (Human)
Length = 662
Score = 43.6 bits (98), Expect = 0.004
Identities = 23/46 (50%), Positives = 28/46 (60%), Gaps = 6/46 (13%)
Frame = +2
Query: 131 MSNVTNQNGTGLEQQLAGLDLQPQ------APKSTGRYIPPHLRRQ 250
MS+V +N GL+QQ AGLDL + S GRYIPPHLR +
Sbjct: 1 MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNR 46
>UniRef50_Q1DGU8 Cluster: Catrin, putative; n=2; Culicidae|Rep:
Catrin, putative - Aedes aegypti (Yellowfever mosquito)
Length = 256
Score = 34.7 bits (76), Expect = 1.9
Identities = 23/67 (34%), Positives = 35/67 (52%)
Frame = +2
Query: 89 LEQSSQKNVPLGXNMSNVTNQNGTGLEQQLAGLDLQPQAPKSTGRYIPPHLRRQLQATSD 268
L ++ ++N N+ +++ N TG Q LD +P PKS G+ P RQ TSD
Sbjct: 13 LNKAPRRNTT-ATNLDDISF-NDTGQTQDQ--LDNRPLLPKSLGQSTPDRKDRQKNGTSD 68
Query: 269 QGEESKR 289
GE+S +
Sbjct: 69 GGEDSPK 75
>UniRef50_Q2QNM2 Cluster: Expressed protein; n=4; Magnoliophyta|Rep:
Expressed protein - Oryza sativa subsp. japonica (Rice)
Length = 191
Score = 33.9 bits (74), Expect = 3.4
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +2
Query: 173 QLAGLDLQPQAPKSTGRYIPPHLRRQLQATSDQGEESKRSSLXTRPS 313
+L+G+D+ P P ++ IP H R+ +D GE+SK +PS
Sbjct: 111 RLSGIDMMPPPPSTS---IPSHQERERNMAADGGEKSKEIDTPEKPS 154
>UniRef50_A1RQW7 Cluster: Putative uncharacterized protein; n=2;
Pyrobaculum|Rep: Putative uncharacterized protein -
Pyrobaculum islandicum (strain DSM 4184 / JCM 9189)
Length = 467
Score = 33.9 bits (74), Expect = 3.4
Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
Frame = -1
Query: 641 IGTLFSSWLRLFPAVIPWCRWFIILLTSH--VARPASVPFAFTIIPFVGFI 495
IG S W+R F VI W W ++T + +A + FAFT+ F+G +
Sbjct: 92 IGIKPSDWVRGF-TVIYWLWWICAIITGYKPIASVVTTEFAFTLALFIGIL 141
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 532,488,924
Number of Sequences: 1657284
Number of extensions: 9526032
Number of successful extensions: 27338
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 26499
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27326
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 48760335122
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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