Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_L03
(612 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 154 1e-38
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 77 2e-15
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 68 1e-12
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 62 5e-11
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 56 4e-09
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 42 9e-05
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 36 0.006
SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2 |Sc... 29 0.53
SPAC3A12.08 |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.70
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 28 0.93
SPCC1753.02c |git3||G-protein coupled receptor Git3|Schizosaccha... 28 1.2
SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr... 27 2.1
SPBC800.10c |||EPS15 repeat family actin cortical patch componen... 27 2.1
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 27 2.8
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy... 27 2.8
SPAC57A7.06 |||U3 snoRNP protein Utp14 |Schizosaccharomyces pomb... 27 2.8
SPBC56F2.08c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 26 3.7
SPBC2G5.07c |rpc25||DNA-directed RNA polymerase III complex subu... 26 4.9
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 6.5
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 154 bits (373), Expect = 1e-38
Identities = 72/89 (80%), Positives = 80/89 (89%)
Frame = +3
Query: 342 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVXADGNGTI 521
LT+EQIAEF+EAFSLFD+D DG IT+ ELG VMRSLGQ+PT AELQDMINEV ADGNGTI
Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65
Query: 522 DFPEFLTMMARKMNDTDSEEEIREGFRVF 608
DF EFLTMMARKM DTD+EEE+RE F+VF
Sbjct: 66 DFTEFLTMMARKMKDTDNEEEVREAFKVF 94
Score = 67.3 bits (157), Expect = 2e-12
Identities = 33/76 (43%), Positives = 50/76 (65%)
Frame = +3
Query: 330 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVXADG 509
M D EE++ +EAF +FDKDG+G IT +EL V+ SLG+ ++ E+ DMI E DG
Sbjct: 78 MKDTDNEEEV---REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDG 134
Query: 510 NGTIDFPEFLTMMARK 557
+G I++ EF +++ K
Sbjct: 135 DGVINYEEFSRVISSK 150
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 77.4 bits (182), Expect = 2e-15
Identities = 40/88 (45%), Positives = 58/88 (65%)
Frame = +3
Query: 345 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVXADGNGTID 524
++EQ E KEAF L+D D DG I T +G+V+RSLG N T+AEL + NE+ ID
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAID 59
Query: 525 FPEFLTMMARKMNDTDSEEEIREGFRVF 608
+F++ ++ K+ +T+SEEE + FRVF
Sbjct: 60 EKKFMSFVSNKLRETESEEEYIKAFRVF 87
Score = 49.6 bits (113), Expect = 4e-07
Identities = 25/79 (31%), Positives = 46/79 (58%), Gaps = 1/79 (1%)
Frame = +3
Query: 324 STMADQLTE-EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVX 500
S ++++L E E E+ +AF +FDKD G I T + M++LG+ ++ E+Q M+ E
Sbjct: 65 SFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEAD 124
Query: 501 ADGNGTIDFPEFLTMMARK 557
+G+ D+ +F+ + K
Sbjct: 125 PTNSGSFDYYDFVQRIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 67.7 bits (158), Expect = 1e-12
Identities = 32/90 (35%), Positives = 50/90 (55%)
Frame = +3
Query: 339 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVXADGNGT 518
++TEEQ + EAF LFD D D I EL MR+LG N ++E+ ++ + G G
Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89
Query: 519 IDFPEFLTMMARKMNDTDSEEEIREGFRVF 608
+ +F+ +M K+ + D EEI+ F +F
Sbjct: 90 LQMEDFVRVMTEKIVERDPLEEIKRAFELF 119
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 62.5 bits (145), Expect = 5e-11
Identities = 35/90 (38%), Positives = 48/90 (53%)
Frame = +3
Query: 339 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVXADGNGT 518
QLT QI E KEAF+L DKDGDG I +++ T++ SL Q+ +E D IN + N
Sbjct: 41 QLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASE----DSINHMFESINPP 96
Query: 519 IDFPEFLTMMARKMNDTDSEEEIREGFRVF 608
I+ FLT M + ++ E F F
Sbjct: 97 INLAAFLTAMGSMLCRISPRNDLLEAFSTF 126
Score = 25.8 bits (54), Expect = 4.9
Identities = 12/46 (26%), Positives = 20/46 (43%)
Frame = +3
Query: 372 EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVXADG 509
EAFS FD G I + + S+G E++ ++ + G
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSYTSHG 166
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 56.0 bits (129), Expect = 4e-09
Identities = 30/83 (36%), Positives = 50/83 (60%), Gaps = 2/83 (2%)
Frame = +3
Query: 366 FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVXADGNGTIDFPEFLTM 545
+K+AFSLFD+ G G I +G ++R+ GQNPT AE+ ++ + + A+ +D +FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAE----VDMEQFLQV 63
Query: 546 MARK--MNDTDSEEEIREGFRVF 608
+ R + EE +GF+VF
Sbjct: 64 LNRPNGFDMPGDPEEFVKGFQVF 86
Score = 41.9 bits (94), Expect = 7e-05
Identities = 20/62 (32%), Positives = 36/62 (58%)
Frame = +3
Query: 363 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVXADGNGTIDFPEFLT 542
EF + F +FDKD G I EL V+ SLG+ + E+ +++ V +G +++ +F+
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVK-DGMVNYHDFVQ 136
Query: 543 MM 548
M+
Sbjct: 137 MI 138
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 41.5 bits (93), Expect = 9e-05
Identities = 22/88 (25%), Positives = 46/88 (52%)
Frame = +3
Query: 345 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVXADGNGTID 524
+ E+I ++ F D + G+I E ++ S+ NP + L +++E DG G +D
Sbjct: 19 SNEEIERIRKRFIKIDANQSGSIDRNEFLSIP-SVASNPLASRLFSVVDE---DGGGDVD 74
Query: 525 FPEFLTMMARKMNDTDSEEEIREGFRVF 608
F EF+ ++ + EE+++ F+++
Sbjct: 75 FQEFINSLSVFSVHGNKEEKLKFAFKIY 102
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 35.5 bits (78), Expect = 0.006
Identities = 23/62 (37%), Positives = 32/62 (51%)
Frame = +3
Query: 351 EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVXADGNGTIDFP 530
E E +EAF LFD G I ++L LG+N T+ +LQ M++ A NG +
Sbjct: 9 EMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLD--LAGTNGKVSRE 66
Query: 531 EF 536
EF
Sbjct: 67 EF 68
>SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 334
Score = 29.1 bits (62), Expect = 0.53
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 4/72 (5%)
Frame = -2
Query: 257 PTAETILPPSLARVASLRNSGSRFGS----SSAANRSESTTRFVALSYPSFVQREG*FRK 90
P T+LP + + ++L +SGS+ GS S+ N S +TT A SF + +
Sbjct: 188 PHDMTVLPQTDSNTSNLMSSGSQLGSFATASTNGNNSTTTTTSSAAHPGSFHKGTNDYSS 247
Query: 89 LQGGNGLHNDYP 54
GN H+ +P
Sbjct: 248 TLAGNE-HSAFP 258
>SPAC3A12.08 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 214
Score = 28.7 bits (61), Expect = 0.70
Identities = 22/67 (32%), Positives = 29/67 (43%)
Frame = -2
Query: 317 WIIWSYTKKSQTTPQKRPHGPTAETILPPSLARVASLRNSGSRFGSSSAANRSESTTRFV 138
WI K+ T QK HG T+L SL V +LR+ S+ S S R T
Sbjct: 109 WISIGLMGKALTGYQKLAHGGAIATLLIESLETVRNLRS--SQANSQSTQPRDPIPTENF 166
Query: 137 ALSYPSF 117
+ PS+
Sbjct: 167 DVRTPSY 173
>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1011
Score = 28.3 bits (60), Expect = 0.93
Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Frame = +3
Query: 336 DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLG--QNPTEAELQDMINEV 497
D+ E A + +S+FD+D +G IT +E+ +G + A L+D+ + +
Sbjct: 538 DETGEVDNATLEACYSIFDRDLNGDITCEEIELACVEIGKERKSISASLRDLNDSI 593
>SPCC1753.02c |git3||G-protein coupled receptor
Git3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 466
Score = 27.9 bits (59), Expect = 1.2
Identities = 28/88 (31%), Positives = 38/88 (43%), Gaps = 7/88 (7%)
Frame = -2
Query: 302 YTKKSQTTPQKRPHGPTAETILPP-SLARVASLRNSGSRF-----GSSSAANRSESTTRF 141
Y+ +SQ +P RP + LPP S + A+L SG F S + N E T F
Sbjct: 269 YSSQSQPSPYSRPLLSSVHPNLPPGSQSTPANLNQSGIHFEQDFRDSPNRTNGLEDHTSF 328
Query: 140 VALSYPSFVQREG*FRKLQG-GNGLHND 60
LS P +G L GN + +D
Sbjct: 329 -KLSSPLTSDEDGASSVLAAYGNDMQDD 355
>SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 749
Score = 27.1 bits (57), Expect = 2.1
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = -2
Query: 260 GPTAETILPPSLARVASLRNSGSRFGSSSAANRSESTT 147
GP+ T+L PS +R+++ S S RS S T
Sbjct: 394 GPSRSTVLNPSTSRMSNYTGLESHLAQLSFKRRSNSAT 431
>SPBC800.10c |||EPS15 repeat family actin cortical patch component
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1116
Score = 27.1 bits (57), Expect = 2.1
Identities = 10/23 (43%), Positives = 18/23 (78%)
Frame = +2
Query: 170 RPSSIRTAIPSFSARPLARVRAA 238
+PS++RT++PS A P+ +V +A
Sbjct: 474 KPSAVRTSVPSQLAAPIPQVASA 496
>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
zf-fungal binuclear cluster type |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 977
Score = 26.6 bits (56), Expect = 2.8
Identities = 9/27 (33%), Positives = 15/27 (55%)
Frame = -1
Query: 480 LEVRLLWGSVLATSSRCPALWS*WCHR 400
LE++ WGS+ A + +W+ W R
Sbjct: 850 LEMKNYWGSISALCDKMSEIWADWVQR 876
>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 384
Score = 26.6 bits (56), Expect = 2.8
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +2
Query: 167 SRPSSIRTAIPSFSARPLARVRA 235
SR +++RT+IP FS P RV++
Sbjct: 354 SRHTALRTSIPKFSWTPTPRVKS 376
>SPAC57A7.06 |||U3 snoRNP protein Utp14 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 929
Score = 26.6 bits (56), Expect = 2.8
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +3
Query: 480 DMINEVXADGNGTIDFPEFLTMMARKMNDTDSEEEI 587
D N + +D + + D LT A MN++DSE EI
Sbjct: 173 DKENLLESDASSSNDSESELTDSADNMNESDSESEI 208
>SPBC56F2.08c |||RNA-binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 661
Score = 26.2 bits (55), Expect = 3.7
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = -2
Query: 245 TILPPSLARVASLRNSGSRFGSSSAANRSESTTRFVALSYPSFVQ 111
+++ P+LA+ ASL NS SSS R + +YP Q
Sbjct: 573 SLVNPTLAKSASLNNSSLLNPSSSLLRREVPAGKLTMPAYPYTTQ 617
>SPBC2G5.07c |rpc25||DNA-directed RNA polymerase III complex subunit
Rpc25|Schizosaccharomyces pombe|chr 2|||Manual
Length = 203
Score = 25.8 bits (54), Expect = 4.9
Identities = 9/19 (47%), Positives = 11/19 (57%), Gaps = 2/19 (10%)
Frame = +1
Query: 7 FNPCVVRPE--TWTWTFRG 57
F+PCV RP+ W W G
Sbjct: 115 FDPCVFRPDERAWVWKIEG 133
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 25.4 bits (53), Expect = 6.5
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +3
Query: 372 EAFSLFDKDGDGTITTKELGTVMR 443
+ F FD+D DG + +EL + R
Sbjct: 311 DLFYQFDRDNDGALNNEELSALFR 334
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,220,219
Number of Sequences: 5004
Number of extensions: 41457
Number of successful extensions: 139
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 267622334
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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