Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_K17
(652 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g22060.1 68414.m02759 expressed protein 31 0.88
At5g47420.1 68418.m05843 expressed protein contains Pfam domain,... 29 2.0
At5g08110.1 68418.m00946 DEAD/DEAH box helicase, putative severa... 29 2.0
At4g17420.1 68417.m02608 expressed protein contains Pfam domain,... 29 2.7
At3g18930.2 68416.m02403 zinc finger (C3HC4-type RING finger) fa... 28 4.7
At3g18930.1 68416.m02402 zinc finger (C3HC4-type RING finger) fa... 28 4.7
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 28 4.7
At5g57230.1 68418.m07150 expressed protein 28 6.2
At3g42800.1 68416.m04480 expressed protein hypothetical proteins... 28 6.2
At3g18370.1 68416.m02336 C2 domain-containing protein contains P... 28 6.2
At5g25060.1 68418.m02970 RNA recognition motif (RRM)-containing ... 27 8.2
At4g34920.1 68417.m04951 1-phosphatidylinositol phosphodiesteras... 27 8.2
At3g21300.1 68416.m02691 RNA methyltransferase family protein co... 27 8.2
At2g28030.1 68415.m03397 aspartyl protease family protein contai... 27 8.2
>At1g22060.1 68414.m02759 expressed protein
Length = 1999
Score = 30.7 bits (66), Expect = 0.88
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +3
Query: 168 ESMLDTHSLWSNLANEMQHLDNMMKELSLKFPXIINE 278
E MLDT +S++ E++ + + +LSLKF + E
Sbjct: 1932 EEMLDTKGRYSSMETELREMHDRYSQLSLKFAEVEGE 1968
>At5g47420.1 68418.m05843 expressed protein contains Pfam domain,
PF01987: Protein of unknown function
Length = 282
Score = 29.5 bits (63), Expect = 2.0
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 141 TTPFSPYVRESMLDTHSLWSNLANEMQHLDNMMK 242
+TPF PYV +S DT + + L E Q + M+K
Sbjct: 7 STPFQPYVYQSQQDTITPFQILGGESQVVQIMLK 40
>At5g08110.1 68418.m00946 DEAD/DEAH box helicase, putative several
putative ATP-dependent helicases
Length = 1058
Score = 29.5 bits (63), Expect = 2.0
Identities = 21/98 (21%), Positives = 48/98 (48%), Gaps = 2/98 (2%)
Frame = +3
Query: 285 VEGDKYXISIHLPGYEQKDINVKAKNGVLMVQANSAFNHYLKIQNLPWDVNSEGSWVYEK 464
+ G+ + + G +D+ K+ ++S F+ Y+K + L D + +
Sbjct: 20 INGESTTVQVSSDG-TIRDLKKALKSCFPPASSSSNFHLYIKGEKLKLDTRVAAITINDG 78
Query: 465 DVLKITFPLKQKQPEDSKRP-VAXPTXTTS-TNVSREE 572
D+L + FP K+K+ + +P ++ P+ +S T+ ++E
Sbjct: 79 DILGL-FPFKKKELRQTPKPDLSKPSSLSSRTSTMKDE 115
>At4g17420.1 68417.m02608 expressed protein contains Pfam domain,
PF01987: Protein of unknown function
Length = 285
Score = 29.1 bits (62), Expect = 2.7
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 141 TTPFSPYVRESMLDTHSLWSNLANEMQHLDNMMK 242
+TPF PYV +S DT + + L E Q + M+K
Sbjct: 7 STPFQPYVYQSQEDTITPFQILGGEAQVVQIMLK 40
>At3g18930.2 68416.m02403 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 Zinc
finger, C3HC4 type (RING finger)
Length = 411
Score = 28.3 bits (60), Expect = 4.7
Identities = 12/33 (36%), Positives = 15/33 (45%)
Frame = +3
Query: 66 CADCWRRSRPRHSTTMARHIGRITITTPFSPYV 164
C D W RS P +G + TP SP+V
Sbjct: 183 CIDEWLRSHPNCPLCRTAILGSAGVLTPMSPFV 215
>At3g18930.1 68416.m02402 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 Zinc
finger, C3HC4 type (RING finger)
Length = 411
Score = 28.3 bits (60), Expect = 4.7
Identities = 12/33 (36%), Positives = 15/33 (45%)
Frame = +3
Query: 66 CADCWRRSRPRHSTTMARHIGRITITTPFSPYV 164
C D W RS P +G + TP SP+V
Sbjct: 183 CIDEWLRSHPNCPLCRTAILGSAGVLTPMSPFV 215
>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
protein-like (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 232
Score = 28.3 bits (60), Expect = 4.7
Identities = 12/79 (15%), Positives = 39/79 (49%)
Frame = +3
Query: 150 FSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPXIINEGRVEGDKYXISIHLPGY 329
F+P + E T + + L ++++ + ++ ++ + + + D Y + +PG
Sbjct: 88 FTPTLNEFFPPT--IGNTLIQATENMNRIFDNFNVNPFQLMGQVKEQDDCYKLRYEVPGL 145
Query: 330 EQKDINVKAKNGVLMVQAN 386
++D+ + +G+L ++ +
Sbjct: 146 TKEDVKITVNDGILTIKGD 164
>At5g57230.1 68418.m07150 expressed protein
Length = 160
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +3
Query: 207 ANEMQHLDNMMKELSLKFPXIINEGRVEGDKY 302
+N QHLD M++E + +F I RVE KY
Sbjct: 60 SNYTQHLDRMLEEAAAEFYPNIKFMRVECPKY 91
>At3g42800.1 68416.m04480 expressed protein hypothetical proteins -
Arabidopsis thaliana; expression supported by MPSS
Length = 341
Score = 27.9 bits (59), Expect = 6.2
Identities = 18/45 (40%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = -1
Query: 619 KPTSTSRTLLSVVNSI-SSRLTFVEVVSVGXATGLLLSSGCFCFS 488
+P S L S +NSI S V++ SVG T + SS CFS
Sbjct: 155 QPVSPGARLTSFLNSIFQSNAKKVKLCSVGKTTDVKSSSSKSCFS 199
>At3g18370.1 68416.m02336 C2 domain-containing protein contains Pfam
profile: PF00168 C2 domain
Length = 815
Score = 27.9 bits (59), Expect = 6.2
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +3
Query: 210 NEMQHLDNMMKELSLKFPXIINEGRVEG 293
N+ L N MK++ +KF +I++G +EG
Sbjct: 735 NKALLLSNQMKQVMIKFQNLIDDGDLEG 762
>At5g25060.1 68418.m02970 RNA recognition motif (RRM)-containing
protein KIAA0332 - Homo sapiens, EMBL:AB002330
Length = 946
Score = 27.5 bits (58), Expect = 8.2
Identities = 9/26 (34%), Positives = 15/26 (57%)
Frame = +3
Query: 186 HSLWSNLANEMQHLDNMMKELSLKFP 263
HS W + +E +H+ N E+ +K P
Sbjct: 686 HSTWEEVKSEREHIKNSYAEVEMKEP 711
>At4g34920.1 68417.m04951 1-phosphatidylinositol
phosphodiesterase-related contains weak similarity to
1-phosphatidylinositol phosphodiesterase precursor (EC
3.1.4.10) (Phosphatidylinositol-specific phospholipase
C) (PI-PLC). (Swiss-Prot:P34024) [Listeria
monocytogenes]
Length = 318
Score = 27.5 bits (58), Expect = 8.2
Identities = 10/36 (27%), Positives = 19/36 (52%)
Frame = +3
Query: 153 SPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKF 260
S Y++++ +DT W+ + ++HL S KF
Sbjct: 209 SDYLKDNWIDTDLPWTKFQSNLKHLSEQQPTSSRKF 244
>At3g21300.1 68416.m02691 RNA methyltransferase family protein
contains TIGRfam TIGR00479: RNA methyltransferase, TrmA
family
Length = 554
Score = 27.5 bits (58), Expect = 8.2
Identities = 21/90 (23%), Positives = 44/90 (48%), Gaps = 1/90 (1%)
Frame = +3
Query: 84 RSRPRHSTTMARHIGRITITTPFSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFP 263
R++ + RH+GR + P V + ++ + + N+M+ + L ++
Sbjct: 160 RAKEEQVHELIRHVGRFSDNNPGLEIVLKPIVAC-DIQFHYRNKMEFSFGPQRWLPIE-- 216
Query: 264 XIINEGRVEGDK-YXISIHLPGYEQKDINV 350
++NE R +G K + + +H PG+ K +NV
Sbjct: 217 -MLNE-RQDGPKNFALGLHAPGFFDKVLNV 244
>At2g28030.1 68415.m03397 aspartyl protease family protein contains
Pfam domain, PF00026: eukaryotic aspartyl protease
Length = 392
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = -2
Query: 102 CGAAETAASSPHNTKAIILVERSNNSQSILT 10
C T ASSPH ++ RSN+S S L+
Sbjct: 11 CSLFTTTASSPHGFTIDLIQRRSNSSSSRLS 41
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,480,233
Number of Sequences: 28952
Number of extensions: 256129
Number of successful extensions: 822
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 802
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 819
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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