BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P04_F_J18 (653 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC652.01 |||BC10 family protein|Schizosaccharomyces pombe|chr ... 32 0.063 SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 27 2.4 SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 26 5.5 SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po... 25 9.5 >SPAC652.01 |||BC10 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 108 Score = 32.3 bits (70), Expect = 0.063 Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 1/57 (1%) Frame = +1 Query: 241 LFVFKIRFVRHHCLYNILILYLLFRVNLLWRESVFFVLCRN-FVTKTLXNRRRFEXY 408 +F+ + ++ C Y ++IL LL LW +S FF RN F TK + Y Sbjct: 24 IFMLSMIARKYPCPYCMIILGLLINTRTLWSDSTFFSFNRNIFDTKAFPTELKEAGY 80 >SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltransferase Pis1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 27.1 bits (57), Expect = 2.4 Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 2/45 (4%) Frame = -2 Query: 250 IQINKLSNEFPEILGETLLFAIDIF--HLFLKRQLVFVILFPNYF 122 ++ KLSN+FP G+ + +D F L L VF++L YF Sbjct: 3 VKFLKLSNDFPFCSGQAMSGVLDTFILTLCLGFTRVFLVLISLYF 47 >SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1029 Score = 25.8 bits (54), Expect = 5.5 Identities = 14/41 (34%), Positives = 19/41 (46%) Frame = +3 Query: 528 TMKRSRHRWLGFTIFVCLLXILASELPNMRIEGTPDFSNTT 650 T+ S WL LL L S + + RIEGT + T+ Sbjct: 153 TLSNSNDSWLWQRFSSLLLNCLVSSINSHRIEGTDTSAETS 193 >SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces pombe|chr 2|||Manual Length = 1014 Score = 25.0 bits (52), Expect = 9.5 Identities = 10/36 (27%), Positives = 18/36 (50%) Frame = +3 Query: 366 CYEDIXEQKAF*XIFWTNLTTETLATSPRFASETLK 473 C+ D+ ++W + T+ +P A+ETLK Sbjct: 450 CWSDMFHDFQNQALYWISRYISTMKYNPELAAETLK 485 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,400,055 Number of Sequences: 5004 Number of extensions: 44465 Number of successful extensions: 98 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 97 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 98 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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