BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_J14
(651 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC823.04 |||rRNA processing protein, DUF947|Schizosaccharomyce... 29 0.58
SPCC338.13 |cog4||Golgi transport complex subunit Cog4 |Schizosa... 28 1.3
SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|... 27 2.3
SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 26 4.1
SPAPB17E12.10c |||SAM-dependent methyltransferase|Schizosaccharo... 26 4.1
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 26 5.4
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 26 5.4
SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/ diacylg... 25 7.2
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 25 7.2
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 25 9.5
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 25 9.5
>SPAC823.04 |||rRNA processing protein, DUF947|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 189
Score = 29.1 bits (62), Expect = 0.58
Identities = 18/74 (24%), Positives = 34/74 (45%), Gaps = 1/74 (1%)
Frame = +1
Query: 67 RHDVHLLARSLIYNFKLYNKVNSETSKSDIFE**NQRKQSKRSDK-RDFHFAKTLSQI*T 243
RH A +++ F+ K + K + N++K+ + DK + K L +
Sbjct: 104 RHLEEERAERVMHEFRAQEKERVKEGKKPFYLKRNEQKKLIQMDKYKSMEGTKALDKYIE 163
Query: 244 RRREKEAKMSKKHL 285
++R + A+ KKHL
Sbjct: 164 KKRRRRAQKEKKHL 177
>SPCC338.13 |cog4||Golgi transport complex subunit Cog4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 738
Score = 27.9 bits (59), Expect = 1.3
Identities = 21/63 (33%), Positives = 31/63 (49%), Gaps = 1/63 (1%)
Frame = -1
Query: 240 LNLR*SFRKM-KISFVAPF*LFSLILLFENITFRCFGVNFVVKFEIINKRTRQQVYVVPV 64
L L +F + K SF F +F L + + I G+N+V+ +E K T + VV
Sbjct: 555 LQLTVNFENLCKTSFNMYFPIFLLPRIEQCIDDSFDGINYVLSYEDYTKETEHERLVVTR 614
Query: 63 LRS 55
LRS
Sbjct: 615 LRS 617
>SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|chr
1|||Manual
Length = 270
Score = 27.1 bits (57), Expect = 2.3
Identities = 14/54 (25%), Positives = 27/54 (50%)
Frame = -3
Query: 304 VSPPDCEGVFSTFLPLFLFASFKFAIKFSQNENLVCRSVLIVFVDFIIRKYHFS 143
+ P C+ + T+LPLF ++ + K + N + + L++F+ Y FS
Sbjct: 81 IDPSICKWILDTYLPLFKKSNNAYPSKIAFNP--ISNTTLVLFLTECCNIYLFS 132
>SPAC3A11.05c |kms1||meiotic spindle pole body protein
Kms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 607
Score = 26.2 bits (55), Expect = 4.1
Identities = 20/60 (33%), Positives = 28/60 (46%)
Frame = +1
Query: 457 SDDHQAFRITVVNNKIPHRIVFMLRKTYLSTVMKKHALPLYITVLFRNIVTTLDYILLAK 636
S D A + TV N PH + + L +VMK + L L+ + LDYIL A+
Sbjct: 519 SKDFMASQDTV-NADNPHFLATKGQPLLLLSVMKSNILRLFYVLFLFACFYGLDYILCAE 577
>SPAPB17E12.10c |||SAM-dependent
methyltransferase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 301
Score = 26.2 bits (55), Expect = 4.1
Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +1
Query: 250 REKEAKMSKKHLHNQAAIPLAPAVFKKPQLRPMIAEYDSKKKGVXNDLS-DVVM 408
R + + + HN A+IP +VF++ E D+K+K DLS DV+M
Sbjct: 139 RSRNSFFRNQQDHNSASIPYLQSVFEE--------ERDTKEKAKIEDLSADVIM 184
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 25.8 bits (54), Expect = 5.4
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -3
Query: 256 FLFASFKFAIKFSQNENLVCRSVLIVFVDFIIRKYHFSMF 137
F F F F KFS +++ RS + FV F++ F+ F
Sbjct: 220 FAFNLFSFKNKFSSLRSILFRSEGLSFVQFLVCIAVFATF 259
>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 855
Score = 25.8 bits (54), Expect = 5.4
Identities = 12/22 (54%), Positives = 12/22 (54%)
Frame = +3
Query: 249 KRKRGKNVEKTPSQSGGDTVST 314
KR RG TP QSGG ST
Sbjct: 681 KRNRGVGTSATPKQSGGRKGST 702
>SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/
diacylglycerol ethanolaminesphotranferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 386
Score = 25.4 bits (53), Expect = 7.2
Identities = 8/25 (32%), Positives = 13/25 (52%)
Frame = +2
Query: 44 HYQLDRNTGTTYTCWRVRLFIISNF 118
HY++D Y W + LF+ +F
Sbjct: 71 HYEMDAFPSWVYASWAIGLFLYQSF 95
>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 871
Score = 25.4 bits (53), Expect = 7.2
Identities = 16/57 (28%), Positives = 28/57 (49%)
Frame = +1
Query: 172 QRKQSKRSDKRDFHFAKTLSQI*TRRREKEAKMSKKHLHNQAAIPLAPAVFKKPQLR 342
++K KRSD + + TL+ + + +K K+ KKH + + V K+P R
Sbjct: 222 EKKVKKRSDLGN-KYESTLNSLINKSVKKHNKLVKKHKPLPSTLDYTAYVKKRPTHR 277
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 25.0 bits (52), Expect = 9.5
Identities = 11/26 (42%), Positives = 17/26 (65%), Gaps = 2/26 (7%)
Frame = -2
Query: 386 FXTPFFLESY--SAIMGRSWGFLKTA 315
F FL++Y S++ GRSW F+ +A
Sbjct: 2188 FFISVFLQNYWMSSVYGRSWAFMWSA 2213
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 25.0 bits (52), Expect = 9.5
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = +2
Query: 107 ISNFTTKLTPKHRKVIFSNNKINENNQNGATNEIFILRKLYRKFKR 244
+S F + P + K +F + I N NG + IF LRK F+R
Sbjct: 3238 LSLFADNILPDYCKQLFKEDFIV--NSNGLKSYIFKLRKWRSYFER 3281
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,605,513
Number of Sequences: 5004
Number of extensions: 51771
Number of successful extensions: 148
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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