BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P04_F_J14 (651 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC823.04 |||rRNA processing protein, DUF947|Schizosaccharomyce... 29 0.58 SPCC338.13 |cog4||Golgi transport complex subunit Cog4 |Schizosa... 28 1.3 SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|... 27 2.3 SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 26 4.1 SPAPB17E12.10c |||SAM-dependent methyltransferase|Schizosaccharo... 26 4.1 SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 26 5.4 SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 26 5.4 SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/ diacylg... 25 7.2 SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 25 7.2 SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 25 9.5 SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 25 9.5 >SPAC823.04 |||rRNA processing protein, DUF947|Schizosaccharomyces pombe|chr 1|||Manual Length = 189 Score = 29.1 bits (62), Expect = 0.58 Identities = 18/74 (24%), Positives = 34/74 (45%), Gaps = 1/74 (1%) Frame = +1 Query: 67 RHDVHLLARSLIYNFKLYNKVNSETSKSDIFE**NQRKQSKRSDK-RDFHFAKTLSQI*T 243 RH A +++ F+ K + K + N++K+ + DK + K L + Sbjct: 104 RHLEEERAERVMHEFRAQEKERVKEGKKPFYLKRNEQKKLIQMDKYKSMEGTKALDKYIE 163 Query: 244 RRREKEAKMSKKHL 285 ++R + A+ KKHL Sbjct: 164 KKRRRRAQKEKKHL 177 >SPCC338.13 |cog4||Golgi transport complex subunit Cog4 |Schizosaccharomyces pombe|chr 3|||Manual Length = 738 Score = 27.9 bits (59), Expect = 1.3 Identities = 21/63 (33%), Positives = 31/63 (49%), Gaps = 1/63 (1%) Frame = -1 Query: 240 LNLR*SFRKM-KISFVAPF*LFSLILLFENITFRCFGVNFVVKFEIINKRTRQQVYVVPV 64 L L +F + K SF F +F L + + I G+N+V+ +E K T + VV Sbjct: 555 LQLTVNFENLCKTSFNMYFPIFLLPRIEQCIDDSFDGINYVLSYEDYTKETEHERLVVTR 614 Query: 63 LRS 55 LRS Sbjct: 615 LRS 617 >SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 27.1 bits (57), Expect = 2.3 Identities = 14/54 (25%), Positives = 27/54 (50%) Frame = -3 Query: 304 VSPPDCEGVFSTFLPLFLFASFKFAIKFSQNENLVCRSVLIVFVDFIIRKYHFS 143 + P C+ + T+LPLF ++ + K + N + + L++F+ Y FS Sbjct: 81 IDPSICKWILDTYLPLFKKSNNAYPSKIAFNP--ISNTTLVLFLTECCNIYLFS 132 >SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schizosaccharomyces pombe|chr 1|||Manual Length = 607 Score = 26.2 bits (55), Expect = 4.1 Identities = 20/60 (33%), Positives = 28/60 (46%) Frame = +1 Query: 457 SDDHQAFRITVVNNKIPHRIVFMLRKTYLSTVMKKHALPLYITVLFRNIVTTLDYILLAK 636 S D A + TV N PH + + L +VMK + L L+ + LDYIL A+ Sbjct: 519 SKDFMASQDTV-NADNPHFLATKGQPLLLLSVMKSNILRLFYVLFLFACFYGLDYILCAE 577 >SPAPB17E12.10c |||SAM-dependent methyltransferase|Schizosaccharomyces pombe|chr 1|||Manual Length = 301 Score = 26.2 bits (55), Expect = 4.1 Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 1/54 (1%) Frame = +1 Query: 250 REKEAKMSKKHLHNQAAIPLAPAVFKKPQLRPMIAEYDSKKKGVXNDLS-DVVM 408 R + + + HN A+IP +VF++ E D+K+K DLS DV+M Sbjct: 139 RSRNSFFRNQQDHNSASIPYLQSVFEE--------ERDTKEKAKIEDLSADVIM 184 >SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 25.8 bits (54), Expect = 5.4 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = -3 Query: 256 FLFASFKFAIKFSQNENLVCRSVLIVFVDFIIRKYHFSMF 137 F F F F KFS +++ RS + FV F++ F+ F Sbjct: 220 FAFNLFSFKNKFSSLRSILFRSEGLSFVQFLVCIAVFATF 259 >SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 855 Score = 25.8 bits (54), Expect = 5.4 Identities = 12/22 (54%), Positives = 12/22 (54%) Frame = +3 Query: 249 KRKRGKNVEKTPSQSGGDTVST 314 KR RG TP QSGG ST Sbjct: 681 KRNRGVGTSATPKQSGGRKGST 702 >SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/ diacylglycerol ethanolaminesphotranferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 386 Score = 25.4 bits (53), Expect = 7.2 Identities = 8/25 (32%), Positives = 13/25 (52%) Frame = +2 Query: 44 HYQLDRNTGTTYTCWRVRLFIISNF 118 HY++D Y W + LF+ +F Sbjct: 71 HYEMDAFPSWVYASWAIGLFLYQSF 95 >SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 871 Score = 25.4 bits (53), Expect = 7.2 Identities = 16/57 (28%), Positives = 28/57 (49%) Frame = +1 Query: 172 QRKQSKRSDKRDFHFAKTLSQI*TRRREKEAKMSKKHLHNQAAIPLAPAVFKKPQLR 342 ++K KRSD + + TL+ + + +K K+ KKH + + V K+P R Sbjct: 222 EKKVKKRSDLGN-KYESTLNSLINKSVKKHNKLVKKHKPLPSTLDYTAYVKKRPTHR 277 >SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|Schizosaccharomyces pombe|chr 1|||Manual Length = 2397 Score = 25.0 bits (52), Expect = 9.5 Identities = 11/26 (42%), Positives = 17/26 (65%), Gaps = 2/26 (7%) Frame = -2 Query: 386 FXTPFFLESY--SAIMGRSWGFLKTA 315 F FL++Y S++ GRSW F+ +A Sbjct: 2188 FFISVFLQNYWMSSVYGRSWAFMWSA 2213 >SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 3699 Score = 25.0 bits (52), Expect = 9.5 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = +2 Query: 107 ISNFTTKLTPKHRKVIFSNNKINENNQNGATNEIFILRKLYRKFKR 244 +S F + P + K +F + I N NG + IF LRK F+R Sbjct: 3238 LSLFADNILPDYCKQLFKEDFIV--NSNGLKSYIFKLRKWRSYFER 3281 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,605,513 Number of Sequences: 5004 Number of extensions: 51771 Number of successful extensions: 148 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 148 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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