Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_H23
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53480.1 68418.m06646 importin beta-2, putative similar to im... 81 6e-16
At2g16950.1 68415.m01953 importin beta-2 subunit family protein ... 51 6e-07
At4g27640.1 68417.m03973 importin beta-2 subunit family protein ... 38 0.008
At3g25840.1 68416.m03219 protein kinase family protein contains ... 30 1.5
At3g59990.2 68416.m06698 methionyl aminopeptidase, putative / me... 29 3.6
At3g59990.1 68416.m06697 methionyl aminopeptidase, putative / me... 29 3.6
At2g44180.1 68415.m05496 methionyl aminopeptidase, putative / me... 29 3.6
At2g36730.1 68415.m04506 pentatricopeptide (PPR) repeat-containi... 29 3.6
At2g34280.1 68415.m04194 S locus F-box-related / SLF-related con... 29 3.6
At1g11050.1 68414.m01266 protein kinase family protein contains ... 29 3.6
At5g22740.1 68418.m02656 glycosyl transferase family 2 protein s... 28 4.7
At5g53700.1 68418.m06672 hypothetical protein 28 6.2
At3g27510.1 68416.m03439 DC1 domain-containing protein contains ... 28 6.2
>At5g53480.1 68418.m06646 importin beta-2, putative similar to
importin-beta2 [Oryza sativa (japonica cultivar-group)]
GI:3983665; contains Pfam profile PF03810: Importin-beta
N-terminal domain
Length = 870
Score = 81.0 bits (191), Expect = 6e-16
Identities = 59/177 (33%), Positives = 87/177 (49%), Gaps = 2/177 (1%)
Frame = +2
Query: 128 LTLIQILEKTVSPDRNELEAAVRYLDHAATTNFTTFIKMLSDVLLQGGNSQVARMAAGLQ 307
+ + Q+L S D + A L N F+ L+ L +R AGL
Sbjct: 3 MEVTQLLINAQSIDGTVRKHAEESLKQFQEQNLAGFLLSLAGELANDEKPVDSRKLAGLV 62
Query: 308 LKNHLTSKDPTLKQQYQQRWLALAXDVRLYIKENILPAIGT-XNSRPSSAAQCVAYVAVA 484
LKN L +K+ K + QRWLAL + I+ +L + S+A+Q +A VA
Sbjct: 63 LKNALDAKEQHRKYELVQRWLALDMSTKSQIRAFLLKTLSAPVPDVRSTASQVIAKVAGI 122
Query: 485 ELPVXQWNDLIPILVENVVHVQSTELKKEATLEAIGYICXDIDAEVL-PERSNQILT 652
ELP QW +LI L+ N +H Q K+ATLE +GY+C ++ +V+ E N+ILT
Sbjct: 123 ELPQKQWPELIVSLLSN-IH-QLPAHVKQATLETLGYLCEEVSPDVVEQEHVNKILT 177
>At2g16950.1 68415.m01953 importin beta-2 subunit family protein
similar to SP|Q92973 Importin beta-2 subunit
(Transportin) {Homo sapiens}; contains Pfam profile
PF03810: Importin-beta N-terminal domain
Length = 891
Score = 51.2 bits (117), Expect = 6e-07
Identities = 45/162 (27%), Positives = 76/162 (46%), Gaps = 3/162 (1%)
Frame = +2
Query: 134 LIQILEKTVSPDRNELEAAV-RYLDHAATTNFTTFIKMLSDVLLQG-GNSQVARMAAGLQ 307
+ +LE+ +SP ++ + + L H + F F L +L++ G S R AAGL
Sbjct: 18 ICSLLEQQISPSSVVDKSQIWKQLQHFS--QFPDFNNYLVFILVRAEGKSVEVRQAAGLL 75
Query: 308 LKNHLTSKDPTLKQQYQQRWLALAXDVRLYIKENILPAIGTXNSRPSSAAQCVAYVAVAE 487
LKN+L P++ Q+ Q+ YIK +LP +G + + + V V
Sbjct: 76 LKNNLRGAYPSMTQENQK-----------YIKSELLPCLGAADRNIRTTVGTIISVIVNI 124
Query: 488 LPVXQWNDLIPILVENVVHVQSTELK-KEATLEAIGYICXDI 610
V W++L+P L V + S +L + ++A+ IC DI
Sbjct: 125 EGVSGWHELLPAL---VTCLDSNDLNHMDGAMDALSKICEDI 163
>At4g27640.1 68417.m03973 importin beta-2 subunit family protein low
similarity to importin 4 GI:18700635 from [Homo sapiens]
Length = 1048
Score = 37.5 bits (83), Expect = 0.008
Identities = 17/66 (25%), Positives = 37/66 (56%), Gaps = 2/66 (3%)
Frame = +2
Query: 341 LKQQYQQRWLALAXDVRLYIKENILPAIGTXNSRP--SSAAQCVAYVAVAELPVXQWNDL 514
L+++ W L+ ++ ++K++++ +I NS P ++A V+ VA +P +W DL
Sbjct: 60 LRKRITGHWAKLSPQLKQHVKQSLIESITVENSPPVRRASANVVSVVAKYAVPAGEWPDL 119
Query: 515 IPILVE 532
+ L +
Sbjct: 120 LTFLFQ 125
Score = 29.9 bits (64), Expect = 1.5
Identities = 20/49 (40%), Positives = 28/49 (57%), Gaps = 4/49 (8%)
Frame = +2
Query: 515 IPILVENVVHVQSTELK-KEATLEAIGYI---CXDIDAEVLPERSNQIL 649
+P+L VH QST LK +EA++ A+G I C D+ E L N +L
Sbjct: 336 LPVLEFASVHCQSTNLKFREASVTALGVISEGCFDLMKEKLDTVLNIVL 384
>At3g25840.1 68416.m03219 protein kinase family protein contains
Pfam profile: PF00069 eukaryotic protein kinase domain
Length = 935
Score = 29.9 bits (64), Expect = 1.5
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 92 LKNSETMHAETTLTLIQILEKTVSPDRNELEAAVRYL 202
++N+ETMH + +QIL+K DR + VR+L
Sbjct: 652 IRNNETMHKAGKIE-VQILKKLAGADREDRRHCVRFL 687
>At3g59990.2 68416.m06698 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to Methionine aminopeptidase 2 (EC
3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo
sapiens} SP|P50579; contains Pfam profile PF00557:
metallopeptidase family M24; supporting cDNA
gi|11344921|gb|AF300880.1|AF300880
Length = 439
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +2
Query: 86 HKLKNSETMHAETTLTLIQILEKTVSPDRNELEAAVRYLDHAATTNFTTFIKMLSD 253
H +KN + H L + L T++ + + L RYLD T + +K L D
Sbjct: 343 HYMKNFDAGHVPLRLPRAKQLLATINKNFSTLAFCRRYLDRIGETKYLMALKNLCD 398
>At3g59990.1 68416.m06697 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to Methionine aminopeptidase 2 (EC
3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo
sapiens} SP|P50579; contains Pfam profile PF00557:
metallopeptidase family M24; supporting cDNA
gi|11344921|gb|AF300880.1|AF300880
Length = 439
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +2
Query: 86 HKLKNSETMHAETTLTLIQILEKTVSPDRNELEAAVRYLDHAATTNFTTFIKMLSD 253
H +KN + H L + L T++ + + L RYLD T + +K L D
Sbjct: 343 HYMKNFDAGHVPLRLPRAKQLLATINKNFSTLAFCRRYLDRIGETKYLMALKNLCD 398
>At2g44180.1 68415.m05496 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to SP|P50579 Methionine aminopeptidase
2 (EC 3.4.11.18) (MetAP 2) {Homo sapiens}; contains Pfam
profile PF00557: metallopeptidase family M24
Length = 441
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +2
Query: 86 HKLKNSETMHAETTLTLIQILEKTVSPDRNELEAAVRYLDHAATTNFTTFIKMLSD 253
H +KN + H L + L T++ + + L RYLD T + +K L D
Sbjct: 345 HYMKNYDVGHVPLRLPRAKQLLATINKNFSTLAFCRRYLDRLGETKYLMALKNLCD 400
>At2g36730.1 68415.m04506 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 501
Score = 28.7 bits (61), Expect = 3.6
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +2
Query: 338 TLKQQYQQRWLALAXDVRLYIKENILPAIGTXNSRPSSAAQCVAYVAVAELPVXQWNDLI 517
T +Q Q L D +Y+ N++ GT + +S A+ V + + E V WN ++
Sbjct: 130 TAGRQIQVEVLKHGFDFDVYVGNNLIHLYGTC--KKTSDARKV-FDEMTERNVVSWNSIM 186
Query: 518 PILVEN 535
LVEN
Sbjct: 187 TALVEN 192
>At2g34280.1 68415.m04194 S locus F-box-related / SLF-related
contains Pfam PF00646: F-box domain; contains weak hit
to TIGRFAM TIGR01640 : F-box protein interaction domain;
weakly similar to self-incompatibility (S-) locus F-box
(GI:29420811) [Prunus mume]
Length = 391
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = -3
Query: 397 IKSNIXCQSQPSLLILLFKCRIFRSEMIFQLQTSCHPSHLTVTTLEK 257
+KS + C+S +CR F+ + + SC+P L V+ ++
Sbjct: 18 VKSLLNCKSVSKQWRSTIRCRAFQERQLMHRRQSCNPDVLLVSVADE 64
>At1g11050.1 68414.m01266 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 625
Score = 28.7 bits (61), Expect = 3.6
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +3
Query: 222 ISQHSSKCCPMSFSKVVTVKW 284
++Q S+ CP+ FS V+T+ W
Sbjct: 19 VAQSPSQTCPLDFSHVLTIPW 39
>At5g22740.1 68418.m02656 glycosyl transferase family 2 protein
similar to beta-(1-3)-glucosyl transferase GB:AAC62210
GI:3687658 from [Bradyrhizobium japonicum], cellulose
synthase from Agrobacterium tumeficiens [gi:710492] and
Agrobacterium radiobacter [gi:710493]; contains Pfam
glycosyl transferase, group 2 family protein domain
PF00535
Length = 534
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/31 (41%), Positives = 19/31 (61%), Gaps = 3/31 (9%)
Frame = +2
Query: 326 SKDPTLKQQYQ---QRWLALAXDVRLYIKEN 409
S DPT+KQ + QRW + ++R I+EN
Sbjct: 137 STDPTVKQMVEVECQRWASKGINIRYQIREN 167
>At5g53700.1 68418.m06672 hypothetical protein
Length = 248
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = -3
Query: 331 FRSEMIFQLQTSCHPSHLTVTTLEKDIGQHFDECCEICGGSMVEVSNCC 185
F+ E ++ PS L+ L+ + +HF C E+ G S++ N C
Sbjct: 115 FKGERTLIVKVYDTPSTLSKIDLQIGLCKHFSSCGEVTGISVLVHGNLC 163
>At3g27510.1 68416.m03439 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 566
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/50 (28%), Positives = 24/50 (48%), Gaps = 5/50 (10%)
Frame = -3
Query: 343 KCRIFRSE---MIFQLQTSCHPSHLT--VTTLEKDIGQHFDECCEICGGS 209
+CR+ E +F+ HPSH+ + + + D C+ICGG+
Sbjct: 152 RCRLVIHEGCVSVFESPEIIHPSHVRHPLKLISSGAPDYTDRSCDICGGT 201
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,087,626
Number of Sequences: 28952
Number of extensions: 244467
Number of successful extensions: 642
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 623
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 639
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -