Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_H17
(652 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 3.1
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 3.1
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 5.4
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 26 5.4
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 7.2
SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 25 7.2
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 25 7.2
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 25 9.5
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 25 9.5
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 3.1
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +2
Query: 134 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 253
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 3.1
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +2
Query: 128 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 217
++ DFFK + ++ H TL ++ NSL+
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 5.4
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +2
Query: 98 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 274
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 275 LQQL 286
Q+
Sbjct: 73 NNQI 76
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 25.8 bits (54), Expect = 5.4
Identities = 22/68 (32%), Positives = 34/68 (50%), Gaps = 1/68 (1%)
Frame = +3
Query: 324 DANGKAKEALEQSRQNIERTAEELRKAHPDVXXNATALREKLQAAVQNTV-QESXKLXXK 500
D+ KAK LEQ+R +ERT E A A+ L+ + ++ +V +E L K
Sbjct: 403 DSIKKAKTELEQARIELERTQREGNYA------RASELQYAIIPELERSVPKEEKTLEEK 456
Query: 501 VSSNVHET 524
S VH++
Sbjct: 457 KPSMVHDS 464
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 25.4 bits (53), Expect = 7.2
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +2
Query: 107 LAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTKSKDA 232
L + V R F + +EH+ K+LE+Q + L +SKDA
Sbjct: 193 LKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQSKDA 233
>SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 521
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +3
Query: 312 AALGDANGKAKEALEQSRQNIERTAEE 392
A G NG+AKE E+ R ++T E+
Sbjct: 470 AKQGKGNGRAKETPEEKRARKKKTKED 496
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 25.4 bits (53), Expect = 7.2
Identities = 26/111 (23%), Positives = 49/111 (44%), Gaps = 6/111 (5%)
Frame = +3
Query: 336 KAKEALEQSRQNIERTAEELRKAHPDVXXN---ATALREKLQ--AAVQNTVQESXKLXXK 500
K +E ++ +NI+ E+LRK+ + + A LRE + T++
Sbjct: 679 KKQEDVDSLEKNIQTLKEDLRKSEEALRFSKLEAKNLREVIDNLKGKHETLEAQRNDLHS 738
Query: 501 VSSNVHETNXKLAPKIKAAYDDFAXNTQXVIKKIQEAANAKQ-*ASILNSH 650
S+ TN L+ ++ + +D T V Q++ KQ S++NS+
Sbjct: 739 SLSDAKNTNAILSSELTKSSEDVKRLTANVETLTQDSKAMKQSFTSLVNSY 789
>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/51 (25%), Positives = 26/51 (50%)
Frame = +3
Query: 357 QSRQNIERTAEELRKAHPDVXXNATALREKLQAAVQNTVQESXKLXXKVSS 509
++RQ E A+ +RK+ PDV + ++ +V + +E + +SS
Sbjct: 284 ENRQFSEYVADRIRKSFPDVSFKNLHVLSCMEDSVMSPFRELGSVTSSMSS 334
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 25.0 bits (52), Expect = 9.5
Identities = 16/61 (26%), Positives = 30/61 (49%), Gaps = 3/61 (4%)
Frame = +3
Query: 318 LGDANGKAKEALEQSRQNIERTAEELRKAHPD---VXXNATALREKLQAAVQNTVQESXK 488
+ +A KA+++LEQ+ + E L K H + + +EKL A ++ + S +
Sbjct: 52 INEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEE 111
Query: 489 L 491
L
Sbjct: 112 L 112
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,727,963
Number of Sequences: 5004
Number of extensions: 25853
Number of successful extensions: 98
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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