SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P04_F_H08
         (651 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g37190.1 68417.m05265 expressed protein                             83   1e-16
At1g20010.1 68414.m02506 tubulin beta-5 chain (TUB5) nearly iden...    34   0.071
At5g62700.1 68418.m07868 tubulin beta-2/beta-3 chain (TUB3) near...    34   0.094
At5g62690.1 68418.m07867 tubulin beta-2/beta-3 chain (TUB2) near...    34   0.094
At5g23860.1 68418.m02801 tubulin beta-8 chain (TUB8) (TUBB8) ide...    34   0.094
At4g20890.1 68417.m03029 tubulin beta-9 chain (TUB9) nearly iden...    34   0.094
At2g29550.1 68415.m03589 tubulin beta-7 chain (TUB7) identical t...    34   0.094
At5g44340.1 68418.m05429 tubulin beta-4 chain (TUB4) nearly iden...    33   0.12 
At5g12250.1 68418.m01439 tubulin beta-6 chain (TUB6) nearly iden...    33   0.12 
At1g75780.1 68414.m08801 tubulin beta-1 chain (TUB1) nearly iden...    32   0.29 
At5g05620.1 68418.m00612 tubulin gamma-2 chain / gamma-2 tubulin...    31   0.50 
At3g61650.1 68416.m06909 tubulin gamma-1 chain / gamma-1 tubulin...    31   0.50 
At5g37160.1 68418.m04461 tRNA-splicing endonuclease positive eff...    29   3.5  
At3g01100.1 68416.m00015 early-responsive to dehydration protein...    28   6.2  
At4g29060.1 68417.m04157 elongation factor Ts family protein sim...    27   8.2  

>At4g37190.1 68417.m05265 expressed protein
          Length = 562

 Score = 83.4 bits (197), Expect = 1e-16
 Identities = 58/185 (31%), Positives = 91/185 (49%), Gaps = 20/185 (10%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNIQELSFDYSGTATTD-------LNHDILYREGQTSRNEV 307
           REI+T+Q G ++N+VG+HFWN Q+     +    +D       L+ D+LYR G+T +   
Sbjct: 2   REIVTIQVGEFANFVGSHFWNFQDELLGLASDPESDPIFRNHNLDMDVLYRSGETQQGVA 61

Query: 308 TYTPRLLIADLKGALKTLPQSGGL----SDDAQETDLQW-DTVXKIEXPPPEKNQYLAAI 472
           TYTPRL+  +LKGAL T+   G L    S    ++   W   V      P ++N +L ++
Sbjct: 62  TYTPRLVSVNLKGALGTMSSRGTLYNEGSSSRSDSSATWFGDVDTQRSEPRKRNLFLQSL 121

Query: 473 DSVQTTVADGK------KDY--NLEXNVITWTDYLYPRFHSRTVNVVNEYQHGSNKEAFD 628
              +  V   K      KD    L+  V  WTD+    +H +++  +N     S  +AF+
Sbjct: 122 YEEEHVVGKEKAKEIEDKDIVGCLDEEVECWTDFSKSHYHPQSLYELNGLWMDS--QAFN 179

Query: 629 IYPTG 643
            Y  G
Sbjct: 180 NYGIG 184


>At1g20010.1 68414.m02506 tubulin beta-5 chain (TUB5) nearly
           identical to SP|P29513 Tubulin beta-5 chain {Arabidopsis
           thaliana}
          Length = 449

 Score = 34.3 bits (75), Expect = 0.071
 Identities = 28/83 (33%), Positives = 38/83 (45%), Gaps = 8/83 (9%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNI--QELSFD----YSG-TATTDLNH-DILYREGQTSRNE 304
           REIL +Q G   N +G+ FW +   E   D    YSG TA   L   ++ Y E    R  
Sbjct: 2   REILHIQGGQCGNQIGSKFWEVICDEHGIDSTGRYSGDTADLQLERINVYYNEASGGR-- 59

Query: 305 VTYTPRLLIADLKGALKTLPQSG 373
             Y PR ++ DL+       +SG
Sbjct: 60  --YVPRAVLMDLEPGTMDSIRSG 80


>At5g62700.1 68418.m07868 tubulin beta-2/beta-3 chain (TUB3) nearly
           identical to SP|P29512 Tubulin beta-2/beta-3 chain
           {Arabidopsis thaliana}
          Length = 450

 Score = 33.9 bits (74), Expect = 0.094
 Identities = 26/79 (32%), Positives = 39/79 (49%), Gaps = 8/79 (10%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNI--QELSFDYSG--TATTDLNHD---ILYREGQTSRNEV 307
           REIL +Q G   N +GA FW +   E   D +G  T  +DL  +   + Y E    R   
Sbjct: 2   REILHIQGGQCGNQIGAKFWEVVCAEHGIDPTGRYTGDSDLQLERINVYYNEASCGR--- 58

Query: 308 TYTPRLLIADLK-GALKTL 361
            + PR ++ DL+ G + +L
Sbjct: 59  -FVPRAVLMDLEPGTMDSL 76


>At5g62690.1 68418.m07867 tubulin beta-2/beta-3 chain (TUB2) nearly
           identical to SP|P29512 Tubulin beta-2/beta-3 chain
           {Arabidopsis thaliana}
          Length = 450

 Score = 33.9 bits (74), Expect = 0.094
 Identities = 26/79 (32%), Positives = 39/79 (49%), Gaps = 8/79 (10%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNI--QELSFDYSG--TATTDLNHD---ILYREGQTSRNEV 307
           REIL +Q G   N +GA FW +   E   D +G  T  +DL  +   + Y E    R   
Sbjct: 2   REILHIQGGQCGNQIGAKFWEVVCAEHGIDPTGRYTGDSDLQLERINVYYNEASCGR--- 58

Query: 308 TYTPRLLIADLK-GALKTL 361
            + PR ++ DL+ G + +L
Sbjct: 59  -FVPRAVLMDLEPGTMDSL 76


>At5g23860.1 68418.m02801 tubulin beta-8 chain (TUB8) (TUBB8)
           identical to SP|P29516 Tubulin beta-8 chain {Arabidopsis
           thaliana}; supporting cDNA gi|15451225|gb|AY054693.1|
          Length = 449

 Score = 33.9 bits (74), Expect = 0.094
 Identities = 21/78 (26%), Positives = 32/78 (41%), Gaps = 3/78 (3%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNIQELSFDYSGTATTDLNHDILYREGQTSRNEVT---YTP 319
           REIL +Q G   N +GA FW +         T      +D+         NE +   + P
Sbjct: 2   REILHIQGGQCGNQIGAKFWEVVCAEHGIDSTGRYQGENDLQLERVNVYYNEASCGRFVP 61

Query: 320 RLLIADLKGALKTLPQSG 373
           R ++ DL+       +SG
Sbjct: 62  RAVLMDLEPGTMDSVRSG 79


>At4g20890.1 68417.m03029 tubulin beta-9 chain (TUB9) nearly
           identical to SP|P29517 Tubulin beta-9 chain {Arabidopsis
           thaliana}
          Length = 444

 Score = 33.9 bits (74), Expect = 0.094
 Identities = 25/77 (32%), Positives = 37/77 (48%), Gaps = 6/77 (7%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNI--QELSFDYSGTATTDLNHDILYREGQTSRNEVT---Y 313
           REIL +Q G   N +GA FW +   E   D +G +  D   D+         NE +   Y
Sbjct: 2   REILHIQGGQCGNQIGAKFWEVICGEHGIDQTGQSCGDT--DLQLERINVYFNEASGGKY 59

Query: 314 TPRLLIADLK-GALKTL 361
            PR ++ DL+ G + +L
Sbjct: 60  VPRAVLMDLEPGTMDSL 76


>At2g29550.1 68415.m03589 tubulin beta-7 chain (TUB7) identical to
           GB:M84704 SP|P29515 Tubulin beta-7 chain {Arabidopsis
           thaliana}
          Length = 449

 Score = 33.9 bits (74), Expect = 0.094
 Identities = 24/82 (29%), Positives = 35/82 (42%), Gaps = 7/82 (8%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNIQELSFD------YSGTATTDLNH-DILYREGQTSRNEV 307
           REIL +Q G   N +G+ FW +  L         Y G +   L   ++ Y E    R   
Sbjct: 2   REILHIQGGQCGNQIGSKFWEVVNLEHGIDQTGRYVGDSELQLERVNVYYNEASCGR--- 58

Query: 308 TYTPRLLIADLKGALKTLPQSG 373
            Y PR ++ DL+       +SG
Sbjct: 59  -YVPRAVLMDLEPGTMDSVRSG 79


>At5g44340.1 68418.m05429 tubulin beta-4 chain (TUB4) nearly
           identical to SP|P24636 Tubulin beta-4 chain {Arabidopsis
           thaliana}
          Length = 444

 Score = 33.5 bits (73), Expect = 0.12
 Identities = 24/79 (30%), Positives = 37/79 (46%), Gaps = 8/79 (10%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNI--QELSFDYSGTATTDL-----NHDILYREGQTSRNEV 307
           REIL +Q G   N +GA FW +   E   D++G    D        D+ + E    +   
Sbjct: 2   REILHIQGGQCGNQIGAKFWEVICDEHGIDHTGQYVGDSPLQLERIDVYFNEASGGK--- 58

Query: 308 TYTPRLLIADLK-GALKTL 361
            Y PR ++ DL+ G + +L
Sbjct: 59  -YVPRAVLMDLEPGTMDSL 76


>At5g12250.1 68418.m01439 tubulin beta-6 chain (TUB6) nearly
           identical to SP|P29514 Tubulin beta-6 chain {Arabidopsis
           thaliana}
          Length = 449

 Score = 33.5 bits (73), Expect = 0.12
 Identities = 23/72 (31%), Positives = 33/72 (45%), Gaps = 7/72 (9%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNI--QELSFD----YSGTATTDLNH-DILYREGQTSRNEV 307
           REIL +Q G   N +G+ FW +   E   D    Y G +   L   ++ Y E    R   
Sbjct: 2   REILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGNSDLQLERVNVYYNEASCGR--- 58

Query: 308 TYTPRLLIADLK 343
            Y PR ++ DL+
Sbjct: 59  -YVPRAILMDLE 69


>At1g75780.1 68414.m08801 tubulin beta-1 chain (TUB1) nearly
           identical to SP|P12411 Tubulin beta-1 chain {Arabidopsis
           thaliana}
          Length = 447

 Score = 32.3 bits (70), Expect = 0.29
 Identities = 24/80 (30%), Positives = 36/80 (45%), Gaps = 5/80 (6%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNI--QELSFDYSGTATTDLNHDILYREGQTSRNEVT---Y 313
           REIL +Q G   N +G+ FW +   E   D +G    D + D+         NE +   Y
Sbjct: 2   REILHVQGGQCGNQIGSKFWEVICDEHGVDPTGRYNGD-SADLQLERINVYYNEASGGRY 60

Query: 314 TPRLLIADLKGALKTLPQSG 373
            PR ++ DL+       +SG
Sbjct: 61  VPRAVLMDLEPGTMDSIRSG 80


>At5g05620.1 68418.m00612 tubulin gamma-2 chain / gamma-2 tubulin
           (TUBG2) identical to  SP|P38558 Tubulin gamma-2 chain
           (Gamma-2 tubulin) {Arabidopsis thaliana}
          Length = 474

 Score = 31.5 bits (68), Expect = 0.50
 Identities = 21/71 (29%), Positives = 34/71 (47%), Gaps = 6/71 (8%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNIQELSFDYSGTATTDLNHDILYREGQT------SRNEVT 310
           REI+TLQ G   N +G  FW  ++L  ++ G +   +  D   + G          ++  
Sbjct: 3   REIITLQVGQCGNQIGMEFW--KQLCLEH-GISKDGILEDFATQGGDRKDVFFYQADDQH 59

Query: 311 YTPRLLIADLK 343
           Y PR L+ DL+
Sbjct: 60  YIPRALLIDLE 70


>At3g61650.1 68416.m06909 tubulin gamma-1 chain / gamma-1 tubulin
           (TUBG1) identical to SP|P38557 Tubulin gamma-1 chain
           (Gamma-1 tubulin) {Arabidopsis thaliana}
          Length = 474

 Score = 31.5 bits (68), Expect = 0.50
 Identities = 21/71 (29%), Positives = 34/71 (47%), Gaps = 6/71 (8%)
 Frame = +2

Query: 149 REILTLQFGHYSNYVGAHFWNIQELSFDYSGTATTDLNHDILYREGQT------SRNEVT 310
           REI+TLQ G   N +G  FW  ++L  ++ G +   +  D   + G          ++  
Sbjct: 3   REIITLQVGQCGNQIGMEFW--KQLCLEH-GISKDGILEDFATQGGDRKDVFFYQADDQH 59

Query: 311 YTPRLLIADLK 343
           Y PR L+ DL+
Sbjct: 60  YIPRALLIDLE 70


>At5g37160.1 68418.m04461 tRNA-splicing endonuclease positive
           effector-related contains similarity to SEN1, a positive
           effector of tRNA-splicing endonuclease [Saccharomyces
           cerevisiae] gi|172574|gb|AAB63976
          Length = 871

 Score = 28.7 bits (61), Expect = 3.5
 Identities = 26/102 (25%), Positives = 43/102 (42%), Gaps = 1/102 (0%)
 Frame = +2

Query: 20  ICSYSYKQILNRAPTCYRKTKIFNEE*HTKNCS-CI*FNLKMSTREILTLQFGHYSNYVG 196
           +CS+S K ILN   +  +   I +        S C   +L   TR  L   F   S    
Sbjct: 22  LCSWSLKDILNEDLSKEKIMTIPDRFSSVDEYSQCFVPHLLEETRTELFSSFRSLSKSPV 81

Query: 197 AHFWNIQELSFDYSGTATTDLNHDILYREGQTSRNEVTYTPR 322
           +   +++    +YSG ++    HDI   +    +NE+ Y P+
Sbjct: 82  SRILSVETKVIEYSGRSSIKWFHDIKLMDYADDKNEI-YEPK 122


>At3g01100.1 68416.m00015 early-responsive to dehydration
           protein-related / ERD protein-related low similarity to
           ERD4 protein (early-responsive to dehydration stress)
           [Arabidopsis thaliana] GI:15375406; contains Pfam
           profile PF02714: Domain of unknown function DUF221
          Length = 703

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 13/44 (29%), Positives = 28/44 (63%)
 Frame = +3

Query: 165 YSLVTIQIMLVPIFGIYKSSVLITVVLQLPI*ITIFYTERVKRR 296
           +SLV + I+ V +FG+ +  V  ++ + LP+ +T+ ++   +RR
Sbjct: 608 FSLVLMHIIAVGLFGLKELPVASSLTIPLPV-LTVLFSIYCQRR 650


>At4g29060.1 68417.m04157 elongation factor Ts family protein
           similar to SP|P35019 Elongation factor Ts (EF-Ts)
           {Galdieria sulphuraria}; contains Pfam profiles PF00627:
           UBA/TS-N domain, PF00889: Elongation factor TS, PF00575:
           S1 RNA binding domain
          Length = 953

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 14/38 (36%), Positives = 21/38 (55%)
 Frame = -3

Query: 640 SGIDVKSFLITSVLILIYYIHSSRMKTRIQVICPSDYI 527
           S  D KS  + S   +  YIH SR+   I+V C +D++
Sbjct: 800 SSADKKSSRLASEGRIGSYIHDSRIGVLIEVNCETDFV 837


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,409,506
Number of Sequences: 28952
Number of extensions: 266796
Number of successful extensions: 579
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 564
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 578
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -