Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_H03
(652 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 77 2e-15
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 65 7e-12
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 34 0.020
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 28 1.0
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 28 1.0
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ... 27 2.3
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ... 26 4.1
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 26 4.1
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 26 5.4
SPCC1183.03c |||frataxin homolog|Schizosaccharomyces pombe|chr 3... 25 9.5
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 77.0 bits (181), Expect = 2e-15
Identities = 50/169 (29%), Positives = 87/169 (51%), Gaps = 4/169 (2%)
Frame = +1
Query: 145 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 312
N D KFP K+P F DG L+E+ AIA+Y+A+ +L G +A+V Q+
Sbjct: 38 NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96
Query: 313 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT 492
S+++SEL A + P + +++Q + A++ + D L ++T+LV R+T
Sbjct: 97 CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLT 156
Query: 493 LADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSL 639
LAD+ L +VL S + ++ R++ T+ HQ ++ A+ L
Sbjct: 157 LADIFFTCFLKFGATYVLTKSYLAKYTHIYRYYQTIYHQAKLDAITEPL 205
>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 220
Score = 65.3 bits (152), Expect = 7e-12
Identities = 50/203 (24%), Positives = 94/203 (46%), Gaps = 8/203 (3%)
Frame = +1
Query: 34 GLLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADG 213
G LY++ N R L A+ V + + S D KFP K+P F ADG
Sbjct: 4 GTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIGADG 60
Query: 214 KVLLTESNAIAYYVAN------ESLRGGDLATQARVWQWASWSDSELL-PASCA-WVFPY 369
L + Y+ E L + +A + +W + + +++ P + WV +
Sbjct: 61 FELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWVGMF 120
Query: 370 LGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLD 549
G + + ++ + + + + +LK+ + + RT+LV +R TLAD+ S L F ++D
Sbjct: 121 RGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLFFGSLLRIFFNSIID 180
Query: 550 PSVRSSLINVQRWFLTVAHQPQV 618
R L ++ R+++T+ HQ ++
Sbjct: 181 EKTRKELPHLTRYYITMFHQAKL 203
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 33.9 bits (74), Expect = 0.020
Identities = 21/69 (30%), Positives = 36/69 (52%), Gaps = 1/69 (1%)
Frame = +1
Query: 442 LDGHLLTRTFLVTER-ITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQV 618
LD L + TF+ + I++AD+ V++ + H++ L L NV RWF + HQ V
Sbjct: 128 LDEFLKSSTFIAQDSGISVADLAVYARI-HSYICGLSAKEGYKLNNVCRWFDFIQHQESV 186
Query: 619 SAVVGSLTL 645
S+++
Sbjct: 187 MEAANSMSM 195
>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
Eme1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 28.3 bits (60), Expect = 1.0
Identities = 18/67 (26%), Positives = 32/67 (47%)
Frame = +1
Query: 37 LLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGK 216
LL P F++ KA + QY+ +FG +K ++F K+ ++ F S +
Sbjct: 548 LLEGIPNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHS 606
Query: 217 VLLTESN 237
+L+ SN
Sbjct: 607 ILINTSN 613
>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 28.3 bits (60), Expect = 1.0
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +2
Query: 419 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLS 511
TY PP Y D+FS APS+ + S+S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287
>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 267
Score = 27.1 bits (57), Expect = 2.3
Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = +2
Query: 281 IWLPKPVSGSGHHGLTANYCL-LPALGSSLTLV 376
+W+P PV SG T C+ LP G +T V
Sbjct: 23 LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55
>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 369
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -3
Query: 221 STFPSALSNAGTFPAGNFFKKSSDL 147
+TFP A + GTFP G F S L
Sbjct: 52 ATFPWAQAPVGTFPIGQLFDPVSGL 76
>SPBC16A3.11 |eso1||sister chromatid cohesion protein
Eso1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +1
Query: 232 SNAIAYYVANESLRGGDLATQARVWQWAS 318
S + A Y+ NE+LR G + +R+W AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808
>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 339
Score = 25.8 bits (54), Expect = 5.4
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
Frame = +2
Query: 311 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 418
GH L + C P++GSS L+ +C N R+ V++L
Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251
>SPCC1183.03c |||frataxin homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 158
Score = 25.0 bits (52), Expect = 9.5
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +2
Query: 272 AEEIWLPKPVSGSGHH 319
A +IWL PVSG H+
Sbjct: 101 AHQIWLSSPVSGPKHY 116
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,845,047
Number of Sequences: 5004
Number of extensions: 60070
Number of successful extensions: 197
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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