Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_G23
(504 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 1.2
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 27 1.2
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 2.1
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 2.1
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 2.8
SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 26 2.8
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 26 2.8
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 3.7
SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 25 4.9
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 4.9
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 25 4.9
SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 25 6.5
SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 25 6.5
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 25 8.5
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 27.5 bits (58), Expect = 1.2
Identities = 12/56 (21%), Positives = 27/56 (48%)
Frame = +2
Query: 323 KAXEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESXKLAKKV 490
KA + LE+ ++ E + EE+ H + T+ + + + +QE ++ K +
Sbjct: 353 KACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEKIIQEKSRVDKMI 408
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 27.5 bits (58), Expect = 1.2
Identities = 17/61 (27%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = +2
Query: 305 LGDANGKAXEALEQSRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESXK 475
+ +A KA ++LEQ+ + E L K H + E+ + +EKL A ++ + S +
Sbjct: 52 INEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEE 111
Query: 476 L 478
L
Sbjct: 112 L 112
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 2.1
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +1
Query: 121 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 240
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 2.1
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 115 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 204
++ DFFK + ++ H TL ++ NSL+
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 2.8
Identities = 14/50 (28%), Positives = 23/50 (46%)
Frame = +1
Query: 157 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQCSA 306
+K+ + E+Q +T DFS K + L+AF K L+ S+
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASS 138
>SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 521
Score = 26.2 bits (55), Expect = 2.8
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +2
Query: 293 SSAALGDANGKAXEALEQSRQNIERTAEE 379
SSA G NG+A E E+ R ++T E+
Sbjct: 468 SSAKQGKGNGRAKETPEEKRARKKKTKED 496
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 26.2 bits (55), Expect = 2.8
Identities = 10/24 (41%), Positives = 18/24 (75%)
Frame = +2
Query: 338 LEQSRQNIERTAEELRKAHPDVEK 409
+EQ+R E T E++++A P++EK
Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 3.7
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +1
Query: 85 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 261
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 262 LQQL 273
Q+
Sbjct: 73 NNQI 76
>SPBC649.05 |cut12|stf1|spindle pole body protein Cut12
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 25.4 bits (53), Expect = 4.9
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = +2
Query: 380 LRKAHPDVEKNATALREKLQAAVQNTVQE 466
L AH D+E T++RE+L++ + +E
Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 25.4 bits (53), Expect = 4.9
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +1
Query: 94 LAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTKSKDA 219
L + V R F + +EH+ K+LE+Q + L +SKDA
Sbjct: 193 LKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQSKDA 233
>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 25.4 bits (53), Expect = 4.9
Identities = 13/51 (25%), Positives = 26/51 (50%)
Frame = +2
Query: 344 QSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESXKLAKKVSS 496
++RQ E A+ +RK+ PDV + ++ +V + +E + +SS
Sbjct: 284 ENRQFSEYVADRIRKSFPDVSFKNLHVLSCMEDSVMSPFRELGSVTSSMSS 334
>SPCP1E11.04c |pal1||membrane associated protein Pal1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 25.0 bits (52), Expect = 6.5
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = -2
Query: 341 PKPXWPCRSRLRALHWRLLAKALSCCSTDSEPSFQALLKSCAS 213
P P + +R R+ H ++ S DS PS Q+ LKS S
Sbjct: 96 PPPRYSKIARARSTHVASSSRHRSPSHNDSSPSTQSSLKSRGS 138
>SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux
transporter Bfr1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1530
Score = 25.0 bits (52), Expect = 6.5
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -1
Query: 432 FSRRAVAFFSTSGWALRSSSAVRSMF 355
FSR V FFS AL+S S + +MF
Sbjct: 570 FSRGGVLFFSILFCALQSLSEIANMF 595
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 24.6 bits (51), Expect = 8.5
Identities = 15/59 (25%), Positives = 32/59 (54%)
Frame = +2
Query: 323 KAXEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESXKLAKKVSSN 499
K+ + LE S Q +E E + P+V++ +EK ++ V+ +E K+++ + S+
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKEE-KISRNLRSS 188
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,471,953
Number of Sequences: 5004
Number of extensions: 24455
Number of successful extensions: 116
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 200198394
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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