BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P04_F_G21 (538 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g62400.1 68414.m07040 protein kinase, putative similar to pro... 32 0.28 At3g61960.1 68416.m06959 protein kinase family protein contains ... 30 0.85 At3g52940.2 68416.m05836 C-14 sterol reductase / delta(14)-stero... 29 2.0 At3g52940.1 68416.m05835 C-14 sterol reductase / delta(14)-stero... 29 2.0 At1g60050.1 68414.m06765 nodulin-related low similarity to MtN21... 28 4.5 At3g01720.1 68416.m00107 expressed protein 27 6.0 >At1g62400.1 68414.m07040 protein kinase, putative similar to protein kinase gi|170047|gb|AAA34002; contains protein kinase domain, Pfam:PF00069 Length = 345 Score = 31.9 bits (69), Expect = 0.28 Identities = 12/42 (28%), Positives = 22/42 (52%) Frame = -1 Query: 496 LLSCKHTPASLFVRRFLKCGSIENQINHKSPNSMQIENVLSI 371 + +CK P + ++ G++ +N K P S+ IE VL + Sbjct: 106 IAACKKPPVYCIITEYMSQGNLRMYLNKKEPYSLSIETVLRL 147 >At3g61960.1 68416.m06959 protein kinase family protein contains eukaryotic protein kinase domain, INTERPRO:IPR000719 Length = 626 Score = 30.3 bits (65), Expect = 0.85 Identities = 15/46 (32%), Positives = 25/46 (54%) Frame = -3 Query: 194 LYRMPPTPPIFDGNYLKTIFGKIIRESGKLKFPRHAGTRYYPSYVE 57 L+++ P FDGN +F I+R++ +LKFP +P V+ Sbjct: 199 LFQLVTGKPPFDGNNHIQLFHNIVRDT-ELKFPEDTRNEIHPDCVD 243 >At3g52940.2 68416.m05836 C-14 sterol reductase / delta(14)-sterol reductase / FACKEL (FK) identical to gi:9082182 Length = 262 Score = 29.1 bits (62), Expect = 2.0 Identities = 14/46 (30%), Positives = 26/46 (56%), Gaps = 4/46 (8%) Frame = +3 Query: 306 VFYLFCAHFYLDFFVPGYF----WNNMESTFSICILFGDL*FI*FS 431 ++ +FCA + LD+FV + W+ + ++FGDL +I F+ Sbjct: 179 LYQIFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFT 224 >At3g52940.1 68416.m05835 C-14 sterol reductase / delta(14)-sterol reductase / FACKEL (FK) identical to gi:9082182 Length = 369 Score = 29.1 bits (62), Expect = 2.0 Identities = 14/46 (30%), Positives = 26/46 (56%), Gaps = 4/46 (8%) Frame = +3 Query: 306 VFYLFCAHFYLDFFVPGYF----WNNMESTFSICILFGDL*FI*FS 431 ++ +FCA + LD+FV + W+ + ++FGDL +I F+ Sbjct: 179 LYQIFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFT 224 >At1g60050.1 68414.m06765 nodulin-related low similarity to MtN21 [Medicago truncatula] GI:2598575; contains Pfam profile PF00892: Integral membrane protein Length = 374 Score = 27.9 bits (59), Expect = 4.5 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = -3 Query: 140 IFGKIIRESGKLKFPRHAGTRYYPSYVEFRL*W 42 IFG IIR S ++K + G Y P + F + W Sbjct: 270 IFGSIIRTSVQVKCSKMKGPYYVPLFKPFGILW 302 >At3g01720.1 68416.m00107 expressed protein Length = 802 Score = 27.5 bits (58), Expect = 6.0 Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 1/48 (2%) Frame = +2 Query: 59 LHTTGSNACRRVAETLIFQIREL-FFQILFLNSCRRKLAALEAYGKVL 199 LHT AC +V +I I +L F + +L + A E YGK L Sbjct: 530 LHTRNPEACDKVGGVIIMHIEDLRKFAMYWLLKTQEVRADKEHYGKEL 577 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,723,610 Number of Sequences: 28952 Number of extensions: 240803 Number of successful extensions: 601 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 590 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 601 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 993966856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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