Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_G05
(525 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 144 9e-36
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 143 1e-35
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 30 0.18
SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 28 0.74
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 27 1.7
SPBC106.14c |sda1||SDA1 family protein|Schizosaccharomyces pombe... 26 3.0
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 26 4.0
SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity... 26 4.0
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 5.2
SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|ch... 25 6.9
SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomy... 25 9.1
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 144 bits (348), Expect = 9e-36
Identities = 73/126 (57%), Positives = 93/126 (73%), Gaps = 2/126 (1%)
Frame = +1
Query: 154 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELXNEERNLLSVAYXNVVXARRSS 333
MS +E V AKLAEQAERY++M MK+V + +L EERNLLSVAY N++ ARR+S
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60
Query: 334 WRVISSIEQKTE--GSXRKQQMAKEYRVKVQKELTXICYDVLGLLDKHLIPKASNPXSKV 507
WR+ISSIEQK E G+ R+ + KEYR K++ EL+ IC+DVL +L+KHLIP A+ SKV
Sbjct: 61 WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKV 120
Query: 508 FYLKMK 525
FY KMK
Sbjct: 121 FYYKMK 126
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 143 bits (347), Expect = 1e-35
Identities = 71/125 (56%), Positives = 91/125 (72%), Gaps = 2/125 (1%)
Frame = +1
Query: 157 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELXNEERNLLSVAYXNVVXARRSSW 336
+ +E+ V AKLAEQAERY+ M MK V T EL EERNLLSVAY NV+ ARR+SW
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62
Query: 337 RVISSIEQKTE--GSXRKQQMAKEYRVKVQKELTXICYDVLGLLDKHLIPKASNPXSKVF 510
R++SSIEQK E G+ + ++ KEYR K+++EL IC D+L +L+KHLIP A++ SKVF
Sbjct: 63 RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVF 122
Query: 511 YLKMK 525
Y KMK
Sbjct: 123 YYKMK 127
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 30.3 bits (65), Expect = 0.18
Identities = 23/104 (22%), Positives = 41/104 (39%), Gaps = 2/104 (1%)
Frame = +1
Query: 136 PLPSSTMSVDKE--ELVQRAKLAEQAERYDDMAAAMKEVTETGVELXNEERNLLSVAYXN 309
P+ S TMS E + R + + Y+ MA + E E ++ + LLS Y N
Sbjct: 2997 PIMSITMSDSSAYGEELMRERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDN 3056
Query: 310 VVXARRSSWRVISSIEQKTEGSXRKQQMAKEYRVKVQKELTXIC 441
+R+ + +E+ + EY +++ L C
Sbjct: 3057 EAYQAELFYRLSNCVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100
>SPAC16A10.08c |mug74|SPAC589.01c|sequence
orphan|Schizosaccharomyces pombe|chr 1|||Manual
Length = 285
Score = 28.3 bits (60), Expect = 0.74
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 5/62 (8%)
Frame = +2
Query: 47 FSAREKLILC-----LNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNWPNKLS 211
FS +E ILC L+L+V + ++ S+ + F +PR K N WPN+ S
Sbjct: 39 FSDKENSILCKQLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETS 98
Query: 212 DM 217
+
Sbjct: 99 HL 100
>SPBC211.03c |||guanyl-nucleotide exchange
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1462
Score = 27.1 bits (57), Expect = 1.7
Identities = 15/49 (30%), Positives = 21/49 (42%)
Frame = -1
Query: 186 TLHQFFLVDGHRGRWKRTNSLMPLSEGEKCI*TSKFKHNISFSLALNGY 40
TL FL DG RWK + S+ K +++ +LNGY
Sbjct: 52 TLKDEFLADGLESRWKTNGEKSSSIRYPLVMEFSQLKEDLTNRASLNGY 100
>SPBC106.14c |sda1||SDA1 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 719
Score = 26.2 bits (55), Expect = 3.0
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +2
Query: 23 QPSKPVYPFSAREKLILCLNLL 88
Q + ++PF EK++LCL LL
Sbjct: 87 QKNHTIFPFELCEKIVLCLVLL 108
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 4.0
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +2
Query: 59 EKLILCLNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNW 196
EKL+ C HF +G+ + L RC +T CNVP W
Sbjct: 683 EKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720
>SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ctf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 363
Score = 25.8 bits (54), Expect = 4.0
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +2
Query: 11 PLAPQPSKPVYPFSAREKLILCLNLLVQIHFSPSD 115
PLAP + + FSA+E L + L +H +P +
Sbjct: 213 PLAPSVVQVLSTFSAQELLNMLSKLQTVVHIAPEE 247
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 5.2
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +2
Query: 101 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 223
FSP +K + +L LFH + PS+++ V N + SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201
>SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1374
Score = 25.0 bits (52), Expect = 6.9
Identities = 13/31 (41%), Positives = 17/31 (54%), Gaps = 2/31 (6%)
Frame = +1
Query: 394 AKEYRVKVQKELTXICYDVL--GLLDKHLIP 480
AK R Q +C D++ GL+DKHL P
Sbjct: 590 AKSIRKAKQNAAFIMCLDLIRMGLIDKHLKP 620
>SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 654
Score = 24.6 bits (51), Expect = 9.1
Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = +2
Query: 11 PLAPQPSKPVYPFSAREKLILCLNLLVQIHFSPSDKGISELV-LFH 145
P A QP++ PF++ + +L L+ + P D ISEL+ L H
Sbjct: 185 PFAQQPAEQFSPFNSEYESLLDFRALI-LGNLPVDYKISELLSLIH 229
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,015,145
Number of Sequences: 5004
Number of extensions: 36751
Number of successful extensions: 109
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 214353836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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