Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P04_F_E04
         (460 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein L27|Schizosac...    90   1e-19
SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein L27|Schizosacch...    84   9e-18
SPCC622.18 |rpl6||60S ribosomal protein L6|Schizosaccharomyces p...    31   0.085
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr...    30   0.20 
SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces pomb...    28   0.79 
SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyce...    27   1.0  
SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr 1|||M...    26   2.4  
SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr...    26   3.2  
SPAC6F6.17 |rif1|tap1, tap11, SPAPJ736.01|telomere length regula...    25   4.2  
SPCC11E10.06c |||RNA polymerase II elongator complex subunit Elp...    25   5.6  
SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||...    25   5.6  
SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ...    25   7.3  
SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces pombe...    25   7.3  
SPBC1604.04 |||thiamine pyrophosphate transporter|Schizosaccharo...    25   7.3  
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb...    25   7.3  
SPBC11B10.04c |mrps28||mitochondrial ribosomal protein subunit S...    24   9.7  
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac...    24   9.7  
SPAC23H4.17c |srb10|prk1, cdk8|cyclin-dependent protein kinase S...    24   9.7  
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom...    24   9.7  
SPAC1D4.03c |aut12||autophagy associated protein Aut12|Schizosac...    24   9.7  

>SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein
           L27|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 136

 Score = 90.2 bits (214), Expect = 1e-19
 Identities = 42/87 (48%), Positives = 56/87 (64%), Gaps = 2/87 (2%)
 Frame = +3

Query: 189 PGKVHKRMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEK--FSAKDLKDPAKRKK 362
           P KV K MG  +I +RS++KPF+KVVNYNHLMPTRY ++    K   +A   K+P +R  
Sbjct: 50  PLKVTKSMGAKRIARRSRVKPFIKVVNYNHLMPTRYALELDNLKGLITADTFKEPTQRSA 109

Query: 363 LRFNTRVRFEXRYKSGKNKWFFQKLXF 443
            R   +  FE +Y+SGK+ WFF  L F
Sbjct: 110 ARKTVKKTFEEKYQSGKSAWFFTPLRF 136



 Score = 68.1 bits (159), Expect = 6e-13
 Identities = 27/58 (46%), Positives = 41/58 (70%)
 Frame = +2

Query: 41  MGKIMKPGKVVLVLSGRYAGRKAIVVXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDG 214
           M KI+KPGKV L+  GR+AG+K +++   D+G+   P+GHA VAG++RYP +  +  G
Sbjct: 1   MVKILKPGKVALITRGRFAGKKVVILQAIDQGSKSHPFGHAVVAGVERYPLKVTKSMG 58


>SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein
           L27|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 136

 Score = 84.2 bits (199), Expect = 9e-18
 Identities = 38/87 (43%), Positives = 55/87 (63%), Gaps = 2/87 (2%)
 Frame = +3

Query: 189 PGKVHKRMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEK--FSAKDLKDPAKRKK 362
           P KV K MG  +I KRS++KPF+KV+NYNHLMPTRY ++    K   +     +P++R  
Sbjct: 50  PLKVTKSMGAKRIAKRSRVKPFIKVINYNHLMPTRYALELDNLKGLVTPTTFSEPSQRSA 109

Query: 363 LRFNTRVRFEXRYKSGKNKWFFQKLXF 443
            +   +  FE +Y++GK+ WFF  L F
Sbjct: 110 AKKTVKNTFEEKYQTGKSAWFFTPLRF 136



 Score = 71.7 bits (168), Expect = 5e-14
 Identities = 29/58 (50%), Positives = 42/58 (72%)
 Frame = +2

Query: 41  MGKIMKPGKVVLVLSGRYAGRKAIVVXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDG 214
           M KI+KPGKV LV  GR+AG+K +++ N D+G+   P+GHA VAG++RYP +  +  G
Sbjct: 1   MVKILKPGKVALVTRGRFAGKKVVILQNVDQGSKSHPFGHAVVAGVERYPLKVTKSMG 58


>SPCC622.18 |rpl6||60S ribosomal protein L6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 195

 Score = 31.1 bits (67), Expect = 0.085
 Identities = 10/34 (29%), Positives = 23/34 (67%)
 Frame = +2

Query: 32  PSKMGKIMKPGKVVLVLSGRYAGRKAIVVXNYDE 133
           P+K+   + PG V ++L+GR+ G++ +V+   ++
Sbjct: 45  PTKLRASLAPGTVCILLAGRFRGKRVVVLSQLED 78


>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1024

 Score = 29.9 bits (64), Expect = 0.20
 Identities = 17/52 (32%), Positives = 21/52 (40%)
 Frame = -3

Query: 329 CAEFFKAEVNCITCWHQVIIVYNLHERLYLGPLVDFILSHPLVHFAGGTCRY 174
           CA+F   E N  + WH     YNL  R Y   + D +   P      G C Y
Sbjct: 772 CAKFSLKETNKTSFWHS---SYNLFNRQYHVDMPDNVSDLPQTRLCYGACVY 820


>SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 225

 Score = 27.9 bits (59), Expect = 0.79
 Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%)
 Frame = +3

Query: 213 GKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDF-SFEKFSAKDLKDP 347
           G + I  R K  P  K+VNYNH   +    +  +F K    D+ DP
Sbjct: 28  GNHWIFARFKFYPLNKIVNYNHFHSSSCQSEAKNFYKQFEGDISDP 73


>SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 134

 Score = 27.5 bits (58), Expect = 1.0
 Identities = 11/28 (39%), Positives = 18/28 (64%)
 Frame = +2

Query: 47  KIMKPGKVVLVLSGRYAGRKAIVVXNYD 130
           + ++ G+VVLV  G Y G+ A++V   D
Sbjct: 6   RYVEVGRVVLVTKGEYTGKLAVIVDIVD 33


>SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 757

 Score = 26.2 bits (55), Expect = 2.4
 Identities = 11/27 (40%), Positives = 15/27 (55%)
 Frame = -2

Query: 273 YSLQPSRKALSWTSCGFYSFPSSCALC 193
           YS+ PS  +L W +  + S PS   LC
Sbjct: 16  YSILPSYSSLQWITNAWCSLPSIYILC 42


>SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 872

 Score = 25.8 bits (54), Expect = 3.2
 Identities = 14/34 (41%), Positives = 19/34 (55%)
 Frame = -3

Query: 344 VFQVFCAEFFKAEVNCITCWHQVIIVYNLHERLY 243
           V+QVFC  F  A+   I   +Q++ V NL E  Y
Sbjct: 315 VYQVFCTNFPNAQQLIIDNQNQLLKVANLLEPSY 348


>SPAC6F6.17 |rif1|tap1, tap11, SPAPJ736.01|telomere length regulator
           protein Rif1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1400

 Score = 25.4 bits (53), Expect = 4.2
 Identities = 9/22 (40%), Positives = 13/22 (59%)
 Frame = -1

Query: 76  QHYFTRLHYLTHLGGIFPLLVD 11
           Q YFT ++     G +FP +VD
Sbjct: 803 QQYFTAIYKFEGCGSVFPFVVD 824


>SPCC11E10.06c |||RNA polymerase II elongator complex subunit Elp4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 361

 Score = 25.0 bits (52), Expect = 5.6
 Identities = 18/61 (29%), Positives = 25/61 (40%), Gaps = 3/61 (4%)
 Frame = +3

Query: 192 GKVHKRMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVD---FSFEKFSAKDLKDPAKRKK 362
           G V + +G  +IHK     PF    N N      +TV    F+ E +    L D  K  K
Sbjct: 286 GLVTQPLGLFRIHKLPLALPFTNHANSNEAGDLSFTVSKRRFTIEPWVLPPLDDEQKDTK 345

Query: 363 L 365
           +
Sbjct: 346 I 346


>SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1260

 Score = 25.0 bits (52), Expect = 5.6
 Identities = 11/24 (45%), Positives = 15/24 (62%)
 Frame = +3

Query: 195 KVHKRMGKNKIHKRSKIKPFVKVV 266
           K+ KR+G   I   SK+ P VK+V
Sbjct: 205 KIAKRIGFTDISLSSKLTPMVKIV 228


>SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1337

 Score = 24.6 bits (51), Expect = 7.3
 Identities = 11/39 (28%), Positives = 19/39 (48%)
 Frame = +3

Query: 300 VDFSFEKFSAKDLKDPAKRKKLRFNTRVRFEXRYKSGKN 416
           + F       K L DP+ R+K  F ++   + +Y+  KN
Sbjct: 150 LQFQIPSNEKKSLDDPSPRRKPSFFSKSNLKRKYRYLKN 188


>SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 504

 Score = 24.6 bits (51), Expect = 7.3
 Identities = 14/46 (30%), Positives = 19/46 (41%)
 Frame = +3

Query: 243 IKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRKKLRFNTR 380
           + PF K   Y H + T  +   S  KF   +     K K  R +TR
Sbjct: 39  VSPFFKKERYKHHLETNVSSHQSATKFIEPEPSPTKKTKLTRRDTR 84


>SPBC1604.04 |||thiamine pyrophosphate
           transporter|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 314

 Score = 24.6 bits (51), Expect = 7.3
 Identities = 11/27 (40%), Positives = 13/27 (48%)
 Frame = -2

Query: 300 LYNVLASSDYSLQPSRKALSWTSCGFY 220
           LY  L+ S   + P R    W SC FY
Sbjct: 246 LYRGLSVSMLKVAPGRYECLWLSCHFY 272


>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 432

 Score = 24.6 bits (51), Expect = 7.3
 Identities = 10/34 (29%), Positives = 20/34 (58%)
 Frame = +2

Query: 29  YPSKMGKIMKPGKVVLVLSGRYAGRKAIVVXNYD 130
           Y ++ G +   GK+V+++SG  A  K+ +  + D
Sbjct: 40  YSTESGMLFHAGKLVIIMSGLPARGKSNIAVSID 73


>SPBC11B10.04c |mrps28||mitochondrial ribosomal protein subunit
           S28|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 288

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 12/44 (27%), Positives = 23/44 (52%)
 Frame = +3

Query: 144 TSRTGMLSSLVSTGTPGKVHKRMGKNKIHKRSKIKPFVKVVNYN 275
           TSR   LS         +  KRM +  +H+R ++  +++ VN++
Sbjct: 223 TSRILALSDHCKNHKKDQTGKRMLRYYVHQRQRMLKYLRKVNFD 266


>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
           Smc6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1140

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 10/36 (27%), Positives = 18/36 (50%)
 Frame = -3

Query: 317 FKAEVNCITCWHQVIIVYNLHERLYLGPLVDFILSH 210
           F   V  I C H V  + +   ++  GP ++F++ H
Sbjct: 90  FDNRVGVIECIHLVNFMCHDSLKINFGPRINFVIGH 125


>SPAC23H4.17c |srb10|prk1, cdk8|cyclin-dependent protein kinase
           Srb10 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 352

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 10/21 (47%), Positives = 14/21 (66%)
 Frame = +3

Query: 174 VSTGTPGKVHKRMGKNKIHKR 236
           +S+GT GKV+K +  N   KR
Sbjct: 11  ISSGTYGKVYKAVSSNSNDKR 31


>SPBC354.13 |rga6||GTPase activating protein
           Rga6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 733

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 8/25 (32%), Positives = 16/25 (64%)
 Frame = -3

Query: 326 AEFFKAEVNCITCWHQVIIVYNLHE 252
           A FF  + +C++ + ++   YN+HE
Sbjct: 69  APFFTKQNSCLSRFRELAQTYNIHE 93


>SPAC1D4.03c |aut12||autophagy associated protein
           Aut12|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 11/30 (36%), Positives = 17/30 (56%)
 Frame = +3

Query: 348 AKRKKLRFNTRVRFEXRYKSGKNKWFFQKL 437
           AK  +L  N+R  F+ R   G N+ F ++L
Sbjct: 208 AKAMQLTLNSRPNFDLRRLIGSNEQFLKEL 237


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,809,099
Number of Sequences: 5004
Number of extensions: 35170
Number of successful extensions: 130
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 172312850
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)