Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_E04
(460 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) simi... 82 2e-16
At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C) 80 6e-16
At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A) 79 1e-15
At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) simila... 33 0.093
At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) simila... 30 0.65
At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) simila... 30 0.65
At5g63900.1 68418.m08023 PHD finger family protein contains Pfam... 29 1.5
At1g72440.1 68414.m08377 CCAAT-box-binding transcription factor-... 29 1.5
At4g35120.1 68417.m04992 kelch repeat-containing F-box family pr... 29 2.0
At1g12440.2 68414.m01438 zinc finger (AN1-like) family protein c... 29 2.0
At1g12440.1 68414.m01437 zinc finger (AN1-like) family protein c... 29 2.0
At1g66070.1 68414.m07499 translation initiation factor-related s... 28 2.6
At4g17210.1 68417.m02588 myosin heavy chain-related contains wea... 27 4.6
At1g47310.1 68414.m05238 expressed protein 27 6.1
At5g67570.1 68418.m08520 pentatricopeptide (PPR) repeat-containi... 27 8.1
>At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) similar
to 60S RIBOSOMAL PROTEIN L27 GB:P41101 from [Solanum
tuberosum]
Length = 135
Score = 81.8 bits (193), Expect = 2e-16
Identities = 37/86 (43%), Positives = 54/86 (62%), Gaps = 1/86 (1%)
Frame = +3
Query: 189 PGKVHKRMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD-LKDPAKRKKL 365
P KV ++ K K+S++K F+K+VNY HLMPTRYT+D ++ + D LK K+
Sbjct: 50 PSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTA 109
Query: 366 RFNTRVRFEXRYKSGKNKWFFQKLXF 443
+ + E R+K+GKN+WFF KL F
Sbjct: 110 LKEAKAKLEERFKTGKNRWFFTKLRF 135
Score = 72.5 bits (170), Expect = 1e-13
Identities = 29/71 (40%), Positives = 49/71 (69%)
Frame = +2
Query: 41 MGKIMKPGKVVLVLSGRYAGRKAIVVXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDGKE 220
M K +K K V++L GRYAG+KA+++ ++D+GTSD+ YGH VAG+ +YP + ++D +
Sbjct: 1 MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 221 *NPQEVQDKAF 253
++ + K F
Sbjct: 61 KTAKKSRVKCF 71
>At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C)
Length = 135
Score = 80.2 bits (189), Expect = 6e-16
Identities = 36/86 (41%), Positives = 54/86 (62%), Gaps = 1/86 (1%)
Frame = +3
Query: 189 PGKVHKRMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD-LKDPAKRKKL 365
P KV ++ K K+S++K F+K+VNY HLMPTRYT+D ++ + D L+ K+
Sbjct: 50 PSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAA 109
Query: 366 RFNTRVRFEXRYKSGKNKWFFQKLXF 443
+ + E R+K+GKN+WFF KL F
Sbjct: 110 LKEAKAKLEERFKTGKNRWFFTKLRF 135
Score = 72.1 bits (169), Expect = 2e-13
Identities = 28/71 (39%), Positives = 48/71 (67%)
Frame = +2
Query: 41 MGKIMKPGKVVLVLSGRYAGRKAIVVXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDGKE 220
M K +K K V++L GRYAG+KA+++ ++D+G D+PYGH VAG+ +YP + ++D +
Sbjct: 1 MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 221 *NPQEVQDKAF 253
++ + K F
Sbjct: 61 KTAKKSRVKCF 71
>At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A)
Length = 135
Score = 79.4 bits (187), Expect = 1e-15
Identities = 36/86 (41%), Positives = 52/86 (60%), Gaps = 1/86 (1%)
Frame = +3
Query: 189 PGKVHKRMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEK-FSAKDLKDPAKRKKL 365
P KV ++ K K+S++K F KV+NY H+MPTRYT+D + SA + K+
Sbjct: 50 PSKVIRKDSAKKTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTA 109
Query: 366 RFNTRVRFEXRYKSGKNKWFFQKLXF 443
+ +FE R+K+GKN+WFF KL F
Sbjct: 110 LKEAKAKFEERFKTGKNRWFFTKLRF 135
Score = 75.4 bits (177), Expect = 2e-14
Identities = 31/71 (43%), Positives = 49/71 (69%)
Frame = +2
Query: 41 MGKIMKPGKVVLVLSGRYAGRKAIVVXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDGKE 220
M K MKPGK V++L GRY G+KA++V ++D+GT +K YGH VAG+ +YP + ++D +
Sbjct: 1 MVKCMKPGKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 221 *NPQEVQDKAF 253
++ + K F
Sbjct: 61 KTAKKSRVKCF 71
>At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) similar
to 60S ribosomal protein L6 GI:7208784 from [Cicer
arietinum]
Length = 233
Score = 33.1 bits (72), Expect = 0.093
Identities = 12/42 (28%), Positives = 25/42 (59%)
Frame = +2
Query: 11 VNE*REYPSKMGKIMKPGKVVLVLSGRYAGRKAIVVXNYDEG 136
VN + P+K+ + PG V+++L+GR+ G++ + + G
Sbjct: 75 VNRRKPKPTKLKASITPGTVLIILAGRFKGKRVVFLKQLSSG 116
>At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 30.3 bits (65), Expect = 0.65
Identities = 10/35 (28%), Positives = 22/35 (62%)
Frame = +2
Query: 32 PSKMGKIMKPGKVVLVLSGRYAGRKAIVVXNYDEG 136
P+K+ + PG V+++L+GR+ G++ + + G
Sbjct: 82 PAKLRASITPGTVLIILAGRFKGKRVVFLKQLASG 116
>At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 30.3 bits (65), Expect = 0.65
Identities = 10/35 (28%), Positives = 22/35 (62%)
Frame = +2
Query: 32 PSKMGKIMKPGKVVLVLSGRYAGRKAIVVXNYDEG 136
P+K+ + PG V+++L+GR+ G++ + + G
Sbjct: 82 PTKLRASITPGTVLIILAGRFKGKRVVFLKQLASG 116
>At5g63900.1 68418.m08023 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 557
Score = 29.1 bits (62), Expect = 1.5
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +3
Query: 369 FNTRVRFEXRYKSGKNKWFF 428
+N R + E RYKS K KWF+
Sbjct: 58 YNKRNKKEQRYKSPKGKWFY 77
>At1g72440.1 68414.m08377 CCAAT-box-binding transcription
factor-related similar to CCAAT-box-binding transcription
factor (CCAAT-binding factor) (CBF) (Swiss-Prot:Q03701)
[Homo sapiens], GB:P53569 [Mus musculus]
Length = 1056
Score = 29.1 bits (62), Expect = 1.5
Identities = 17/52 (32%), Positives = 22/52 (42%)
Frame = +3
Query: 207 RMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRKK 362
R K K KR + PF + Y HL+ D K K +P K+KK
Sbjct: 1000 RSKKKKKEKRKRKSPFASLEEYKHLIDQDEKED---SKTKRKATSEPTKKKK 1048
>At4g35120.1 68417.m04992 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 389
Score = 28.7 bits (61), Expect = 2.0
Identities = 20/67 (29%), Positives = 32/67 (47%), Gaps = 3/67 (4%)
Frame = -2
Query: 252 KALSWTSCGFYSFPSSCALCRGYLSIPATKACPYGLSEVPSS*XLTTIAL---RPAYRPL 82
++LS S FYS SS + I AT+ CPY +P T+A + + P+
Sbjct: 48 RSLSLVSKSFYSLLSSTEIYAARSHIGATEPCPYVCLWLPKKHRWFTLAEIEGKLSLEPV 107
Query: 81 KTSTTLP 61
+ S++ P
Sbjct: 108 RLSSSYP 114
>At1g12440.2 68414.m01438 zinc finger (AN1-like) family protein
contains Pfam domains, PF01428: AN1-like Zinc finger and
PF01754: A20-like zinc finger
Length = 168
Score = 28.7 bits (61), Expect = 2.0
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -2
Query: 270 SLQPSRKALSWTSCGFYSFPSSCALC-RGYLSIPATK 163
S PS L CGF+ PS+ LC + Y I AT+
Sbjct: 10 SFSPSEPKLCVKGCGFFGSPSNMNLCSKCYRDIRATE 46
>At1g12440.1 68414.m01437 zinc finger (AN1-like) family protein
contains Pfam domains, PF01428: AN1-like Zinc finger and
PF01754: A20-like zinc finger
Length = 168
Score = 28.7 bits (61), Expect = 2.0
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -2
Query: 270 SLQPSRKALSWTSCGFYSFPSSCALC-RGYLSIPATK 163
S PS L CGF+ PS+ LC + Y I AT+
Sbjct: 10 SFSPSEPKLCVKGCGFFGSPSNMNLCSKCYRDIRATE 46
>At1g66070.1 68414.m07499 translation initiation factor-related
similar to Eukaryotic translation initiation factor 3
subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
(Swiss-Prot:O75822) [Homo sapiens]
Length = 226
Score = 28.3 bits (60), Expect = 2.6
Identities = 18/60 (30%), Positives = 28/60 (46%)
Frame = +3
Query: 240 KIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRKKLRFNTRVRFEXRYKSGKNK 419
+IKP+ K +Y L+ T + S A D+KD A N +++ E +GK K
Sbjct: 139 RIKPYEKSYHYIALLKT--IMRLSLTNMKAADVKDVASSITTIANEKLKAEKEAAAGKKK 196
>At4g17210.1 68417.m02588 myosin heavy chain-related contains weak
similarity to Swiss-Prot:P14105 myosin heavy chain,
nonmuscle (Cellular myosin heavy chain) (NMMHC) [Gallus
gallus]
Length = 527
Score = 27.5 bits (58), Expect = 4.6
Identities = 17/63 (26%), Positives = 29/63 (46%)
Frame = +2
Query: 80 LSGRYAGRKAIVVXNYDEGTSDKPYGHAFVAGIDRYPRQSAQEDGKE*NPQEVQDKAFRE 259
LSG K+ + YDE +S K + +D R++ + GKE QE ++ + E
Sbjct: 292 LSGELKEAKSAMQEIYDEESSYKSLVGSLTVELDGVQRENRELKGKEKERQEAEEGEWVE 351
Query: 260 GCK 268
+
Sbjct: 352 ASR 354
>At1g47310.1 68414.m05238 expressed protein
Length = 395
Score = 27.1 bits (57), Expect = 6.1
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +3
Query: 357 KKLRFNTRVRFEXRYKSGKNKWFF 428
KK+R T RFE R + GK+++ F
Sbjct: 67 KKIRIGTSRRFEIRIRLGKSRFVF 90
>At5g67570.1 68418.m08520 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 611
Score = 26.6 bits (56), Expect = 8.1
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 2/55 (3%)
Frame = +3
Query: 183 GTPGKVHKRMGKNKIHKRSKIKPFVKVVNYN--HLMPTRYTVDFSFEKFSAKDLK 341
GT + K G+N + +K + F ++V+ HL+P YT F E SA+ L+
Sbjct: 517 GTANMMLKVYGRNDMFSEAK-ELFEEIVSRKETHLVPNEYTYSFMLEA-SARSLQ 569
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,424,450
Number of Sequences: 28952
Number of extensions: 181922
Number of successful extensions: 538
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 525
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 534
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 762235320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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