Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_D22
(649 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 283 1e-77
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 154 9e-39
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 151 8e-38
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 149 5e-37
SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit ... 29 0.44
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.3
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.1
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.1
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 4.1
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 5.4
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.1
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 283 bits (695), Expect = 1e-77
Identities = 127/186 (68%), Positives = 146/186 (78%)
Frame = +2
Query: 92 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 271
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 272 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 451
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 452 RKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIXEEYPDRIMNTYSVVPSPKVSDTVX 631
R+EAE+CD LQGFQ KI EEYPDR+M T+SV P+PK SDTV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 632 XPYNAT 649
PYNAT
Sbjct: 181 EPYNAT 186
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 154 bits (374), Expect = 9e-39
Identities = 75/187 (40%), Positives = 106/187 (56%), Gaps = 2/187 (1%)
Frame = +2
Query: 92 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 265
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 266 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 445
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 446 VVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIXEEYPDRIMNTYSVVPSPKVSDT 625
+R+ A++C LQGF ++ EY + +SV P+P+VS +
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 626 VXXPYNA 646
V PYN+
Sbjct: 181 VVEPYNS 187
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 151 bits (366), Expect = 8e-38
Identities = 69/187 (36%), Positives = 113/187 (60%), Gaps = 3/187 (1%)
Frame = +2
Query: 95 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 274
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 275 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 448
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 449 VRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIXEEYPDRIMNTYSVVP-SPKVSDT 625
+ +EA+ D L+GF ++ + YP +I+ TYSV P S VSD
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 626 VXXPYNA 646
V PYN+
Sbjct: 182 VVQPYNS 188
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 149 bits (360), Expect = 5e-37
Identities = 72/191 (37%), Positives = 107/191 (56%), Gaps = 6/191 (3%)
Frame = +2
Query: 92 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 253
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 254 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 433
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 434 SVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIXEEYPDRIMNTYSVVPSPK 613
SVL+ +R+ A++C LQGF ++ EY + +SV P+P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Query: 614 VSDTVXXPYNA 646
VS +V PYN+
Sbjct: 181 VSTSVVEPYNS 191
>SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit
Pmc1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 879
Score = 29.5 bits (63), Expect = 0.44
Identities = 14/40 (35%), Positives = 28/40 (70%)
Frame = -1
Query: 139 DLVAALTSLDMYDFPHFVLFM*TINYVNTKNLLRTLHSLS 20
D+++ TSL + + P L+M + + ++TK +L+TLH+L+
Sbjct: 123 DILSTGTSLRLANAPTSKLYMLSESPLSTKQILQTLHALN 162
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.3
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 131 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 232
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 326 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 427
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2
Query: 395 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 484
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 4.1
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -2
Query: 468 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 331
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 5.4
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 347 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 478
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.1
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 384 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 289
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,370,999
Number of Sequences: 5004
Number of extensions: 44172
Number of successful extensions: 129
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -