BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P04_F_D09 (653 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_8156| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.82 SB_3148| Best HMM Match : Keratin_B2 (HMM E-Value=0.26) 30 1.9 SB_2219| Best HMM Match : 7tm_2 (HMM E-Value=0.81) 29 2.5 >SB_8156| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 228 Score = 31.1 bits (67), Expect = 0.82 Identities = 15/40 (37%), Positives = 23/40 (57%) Frame = +2 Query: 170 ASNATLIGASITYVGALFLLLSFAGPYWMESYPEMFSPFK 289 AS++ LIGA T V + S G +++ +Y +F PFK Sbjct: 187 ASSSCLIGARFTLVALICKRSSIRGSWYLYTYLSIFVPFK 226 >SB_3148| Best HMM Match : Keratin_B2 (HMM E-Value=0.26) Length = 147 Score = 29.9 bits (64), Expect = 1.9 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = -3 Query: 336 PGNLKLSKQYSHNPICLNGENISGYDSIQ*GPANER 229 P + ++ KQ+ H P+C+N NI+ +Q P + R Sbjct: 21 PTSTRVCKQHQHQPVCVNSTNIN--PCVQTAPTSTR 54 Score = 29.1 bits (62), Expect = 3.3 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = -3 Query: 336 PGNLKLSKQYSHNPICLNGENISGYDSIQ*GPANER 229 P + ++ KQ+ H P+C N NI+ +Q P + R Sbjct: 50 PTSTRVCKQHQHQPVCANSTNIN--PCVQTAPTSTR 83 Score = 29.1 bits (62), Expect = 3.3 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = -3 Query: 336 PGNLKLSKQYSHNPICLNGENISGYDSIQ*GPANER 229 P + ++ KQ+ H P+C N NI+ +Q P + R Sbjct: 79 PTSTRVCKQHQHQPVCANSTNIN--PCVQTAPTSTR 112 >SB_2219| Best HMM Match : 7tm_2 (HMM E-Value=0.81) Length = 148 Score = 29.5 bits (63), Expect = 2.5 Identities = 21/79 (26%), Positives = 36/79 (45%), Gaps = 1/79 (1%) Frame = +2 Query: 407 WLLPGWLMAVQFFVTVALMLSFAAQVLLACVIVRWPLXTVLRYEWIFVFYSFIMVAVSSA 586 W+L L +Q ++ + + + A L C I W L V+ +++Y V A Sbjct: 20 WML---LEGIQLYLQLVRVYN-ADMNLKLCYIFAWALVIVVSIYMGYLWYRVTWTVVVPA 75 Query: 587 FLFLAVAIFXGNC-YRRDW 640 L + V+I+ G+ YR W Sbjct: 76 ALVIVVSIYMGHLWYRVTW 94 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 20,721,328 Number of Sequences: 59808 Number of extensions: 454816 Number of successful extensions: 994 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 920 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 992 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1669334250 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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