BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_D09
(653 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_8156| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.82
SB_3148| Best HMM Match : Keratin_B2 (HMM E-Value=0.26) 30 1.9
SB_2219| Best HMM Match : 7tm_2 (HMM E-Value=0.81) 29 2.5
>SB_8156| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 228
Score = 31.1 bits (67), Expect = 0.82
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +2
Query: 170 ASNATLIGASITYVGALFLLLSFAGPYWMESYPEMFSPFK 289
AS++ LIGA T V + S G +++ +Y +F PFK
Sbjct: 187 ASSSCLIGARFTLVALICKRSSIRGSWYLYTYLSIFVPFK 226
>SB_3148| Best HMM Match : Keratin_B2 (HMM E-Value=0.26)
Length = 147
Score = 29.9 bits (64), Expect = 1.9
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = -3
Query: 336 PGNLKLSKQYSHNPICLNGENISGYDSIQ*GPANER 229
P + ++ KQ+ H P+C+N NI+ +Q P + R
Sbjct: 21 PTSTRVCKQHQHQPVCVNSTNIN--PCVQTAPTSTR 54
Score = 29.1 bits (62), Expect = 3.3
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -3
Query: 336 PGNLKLSKQYSHNPICLNGENISGYDSIQ*GPANER 229
P + ++ KQ+ H P+C N NI+ +Q P + R
Sbjct: 50 PTSTRVCKQHQHQPVCANSTNIN--PCVQTAPTSTR 83
Score = 29.1 bits (62), Expect = 3.3
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -3
Query: 336 PGNLKLSKQYSHNPICLNGENISGYDSIQ*GPANER 229
P + ++ KQ+ H P+C N NI+ +Q P + R
Sbjct: 79 PTSTRVCKQHQHQPVCANSTNIN--PCVQTAPTSTR 112
>SB_2219| Best HMM Match : 7tm_2 (HMM E-Value=0.81)
Length = 148
Score = 29.5 bits (63), Expect = 2.5
Identities = 21/79 (26%), Positives = 36/79 (45%), Gaps = 1/79 (1%)
Frame = +2
Query: 407 WLLPGWLMAVQFFVTVALMLSFAAQVLLACVIVRWPLXTVLRYEWIFVFYSFIMVAVSSA 586
W+L L +Q ++ + + + A L C I W L V+ +++Y V A
Sbjct: 20 WML---LEGIQLYLQLVRVYN-ADMNLKLCYIFAWALVIVVSIYMGYLWYRVTWTVVVPA 75
Query: 587 FLFLAVAIFXGNC-YRRDW 640
L + V+I+ G+ YR W
Sbjct: 76 ALVIVVSIYMGHLWYRVTW 94
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,721,328
Number of Sequences: 59808
Number of extensions: 454816
Number of successful extensions: 994
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 920
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 992
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1669334250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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