Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_C21
(583 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomy... 128 8e-31
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 55 7e-09
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 48 1e-06
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 48 1e-06
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 36 0.004
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 29 0.65
>SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 194
Score = 128 bits (308), Expect = 8e-31
Identities = 61/138 (44%), Positives = 87/138 (63%), Gaps = 1/138 (0%)
Frame = +1
Query: 166 LQCLLGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYI 345
++ LL +Q DFV+ A+ S I ++K D+ K ++ +M G++GDT FAEYI
Sbjct: 1 MESLLAVQGQDFVLTASSSSAVRGITVLKPDDDKSQILNSHNLMLYCGEAGDTTNFAEYI 60
Query: 346 AKNIXLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENG-PELYFMD 522
A NI LY +R+ L P A ASFTR+ LA LRS PY VN+L+ GY+ G PEL+++D
Sbjct: 61 AANISLYTLRHNLNLSPEATASFTRKQLATSLRSRKPYQVNILLAGYETNLGKPELFWLD 120
Query: 523 YLXSSVKVPFAAHGYGGY 576
YL + V+VP+A GY +
Sbjct: 121 YLATCVRVPYACQGYSSF 138
>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 262
Score = 55.2 bits (127), Expect = 7e-09
Identities = 38/138 (27%), Positives = 62/138 (44%), Gaps = 2/138 (1%)
Frame = +1
Query: 175 LLGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAK- 351
+L ++ D VMIAAD S+ + D ++ K+ D ++G GD D Q + K
Sbjct: 46 VLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQIQRLLEKL 105
Query: 352 NIXLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKEN-GPELYFMDYL 528
I +GY L PS + + L PY+ +++ G D EN P + F D
Sbjct: 106 EIKEGNYGDGYALQPSYIHEYLSKVLYARRNKLDPYWNQLIVAGVDGENKEPYVAFADLR 165
Query: 529 XSSVKVPFAAHGYGGYLS 582
++ P A G+ +L+
Sbjct: 166 GTTYSAPAIATGFAMHLA 183
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 47.6 bits (108), Expect = 1e-06
Identities = 30/119 (25%), Positives = 54/119 (45%)
Frame = +1
Query: 178 LGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 357
+ I + F ++A D + + I + +++++ D LV+G G D + I + I
Sbjct: 17 VAIAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADALALVKRIQQRI 76
Query: 358 XLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLXS 534
LY + ++ + A R L Y + PY+V + G DKE E+Y D + S
Sbjct: 77 DLYHDNHERKMSAQSCACMVRTLL--YGKRFFPYYVYTTVAGIDKEGKGEIYSFDPVGS 133
>SPAC23D3.07 |pup1||20S proteasome component beta
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 47.6 bits (108), Expect = 1e-06
Identities = 34/132 (25%), Positives = 61/132 (46%)
Frame = +1
Query: 175 LLGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKN 354
++G+ D +++ AD + +I + K++ IS + G + DT I+ N
Sbjct: 38 IVGVIAKDCIVLGADTRATAGPIIADKNCKKLHLISPNIWCAGAGTAADTEFVTSMISSN 97
Query: 355 IXLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLXS 534
I L+ + + A + +++L Y Y V +GGYD + GP L+ + S
Sbjct: 98 IELHSLYTNRKPRVVTALTMLKQHLFRYQGHIGAYLV---LGGYDCK-GPHLFTIAAHGS 153
Query: 535 SVKVPFAAHGYG 570
S K+P+ A G G
Sbjct: 154 SDKLPYVALGSG 165
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 35.9 bits (79), Expect = 0.004
Identities = 26/116 (22%), Positives = 52/116 (44%), Gaps = 4/116 (3%)
Frame = +1
Query: 178 LGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 357
LGI D +++AA++ + ++ ++ K+Y I D ++ V G + D N Y A+ +
Sbjct: 35 LGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILINY-ARRV 93
Query: 358 XLYKMRNGYELGPS----AAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELY 513
++ E P ++ +Y P+ V+ L G+D G +L+
Sbjct: 94 GQQYLQTFNEEMPCEQLVRRVCDLKQGYTQY-GGLRPFGVSFLYAGWDHIRGYQLF 148
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 28.7 bits (61), Expect = 0.65
Identities = 27/123 (21%), Positives = 52/123 (42%)
Frame = +1
Query: 202 VMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIXLYKMRNG 381
+++ D S +I K+ +I+ L+ + G + D + + L+++RN
Sbjct: 73 IVVCVDSRASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRN- 131
Query: 382 YELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLXSSVKVPFAAH 561
EL +AAS N+ Y + ++ G K G LY++D + +K +
Sbjct: 132 KELISVSAASKILSNIT-YSYKGYGLSMGTMLAGTGK-GGTALYYIDSDGTRLKGDLFSV 189
Query: 562 GYG 570
G G
Sbjct: 190 GSG 192
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,215,919
Number of Sequences: 5004
Number of extensions: 41860
Number of successful extensions: 101
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 250133048
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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