Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_C21
(583 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2) (... 134 5e-32
At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1) (P... 134 5e-32
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 57 7e-09
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 46 2e-05
At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1) (... 40 0.001
At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1) (... 39 0.003
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 37 0.009
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 37 0.009
At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1) (... 34 0.060
At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2) ... 34 0.079
At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1) ... 34 0.079
At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1) ... 31 0.74
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ... 29 1.7
At1g48840.1 68414.m05468 expressed protein contains Pfam profile... 28 4.0
At1g19030.1 68414.m02369 hypothetical protein 27 9.1
>At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2)
(PRCGA) identical to SP|O24633 Proteasome subunit beta
type 2-2 (EC 3.4.25.1) (20S proteasome alpha subunit D2)
{Arabidopsis thaliana}, cDNA proteasome subunit prcga
GI:2511571
Length = 199
Score = 134 bits (323), Expect = 5e-32
Identities = 64/139 (46%), Positives = 88/139 (63%)
Frame = +1
Query: 166 LQCLLGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYI 345
++C+ G+ N F ++AAD S HSI++ K+ E KI + ++ G+ GD QF EY+
Sbjct: 1 MECVFGLVGNGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYV 60
Query: 346 AKNIXLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDY 525
KN+ LY+ RNG L +AAA+FTR LA LR + PY VN+LM GYDKE G LY++DY
Sbjct: 61 QKNVSLYQFRNGIPLSTAAAANFTRGELATALRKN-PYSVNILMAGYDKEAGASLYYIDY 119
Query: 526 LXSSVKVPFAAHGYGGYLS 582
+ + KV A GYG Y S
Sbjct: 120 IATLHKVDKGAFGYGSYFS 138
>At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1)
(PRGB) identical to GB:CAA74026 from [Arabidopsis
thaliana] ( FEBS Lett. (1997) 416 (3), 281-285);
identical to cDNA proteasome subunit prgb GI:2511589
Length = 204
Score = 134 bits (323), Expect = 5e-32
Identities = 64/139 (46%), Positives = 89/139 (64%)
Frame = +1
Query: 166 LQCLLGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYI 345
++C+ G+ N F ++AAD S HSI++ K++E KI + ++ G+ GD QF EY+
Sbjct: 1 MECVFGLVGNGFAIVAADTSAVHSILLHKNNEDKIMVLDSHKLVAASGEPGDRVQFTEYV 60
Query: 346 AKNIXLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDY 525
KN+ LYK RNG L +AAA+FTR LA LR + PY VN+LM GYD E+G LY++DY
Sbjct: 61 QKNVSLYKFRNGIPLTTAAAANFTRGELATALRKN-PYSVNILMAGYDDESGASLYYIDY 119
Query: 526 LXSSVKVPFAAHGYGGYLS 582
+ + KV A GYG Y S
Sbjct: 120 IATLHKVDKGAFGYGSYFS 138
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 57.2 bits (132), Expect = 7e-09
Identities = 35/134 (26%), Positives = 62/134 (46%)
Frame = +1
Query: 178 LGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 357
+ I +D+ +IAAD S I+ D KI+K++DR V+ G D + +
Sbjct: 19 VAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSSSGFQADVKALQKVLKSRH 78
Query: 358 XLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLXSS 537
+Y+ ++ ++ A A L Y + PY+ ++GG D+E ++ D + S
Sbjct: 79 LIYQHQHNKQMSCPAMAQLLSNTL--YFKRFFPYYAFNVLGGLDEEGKGCVFTYDAVGSY 136
Query: 538 VKVPFAAHGYGGYL 579
+V + A G G L
Sbjct: 137 ERVGYGAQGSGSTL 150
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 45.6 bits (103), Expect = 2e-05
Identities = 28/132 (21%), Positives = 63/132 (47%)
Frame = +1
Query: 175 LLGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKN 354
++G+ N V++ AD S + + KI +++D + + G + D+ ++Y+
Sbjct: 15 IIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYF 74
Query: 355 IXLYKMRNGYELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLXS 534
+ + +++G +A+ R + Y + +++GG+DK G ++Y + +
Sbjct: 75 LHQHTIQHGQPATVKVSANLIR--MLAY-NNKNMLQTGLIVGGWDKYEGGKIYGIPLGGT 131
Query: 535 SVKVPFAAHGYG 570
V+ PFA G G
Sbjct: 132 VVEQPFAIGGSG 143
>At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1)
(PRC8) identical to proteasome subunit alpha type 3
SP:O23715, GI:12644056 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc8 GI:2511591
Length = 249
Score = 39.9 bits (89), Expect = 0.001
Identities = 36/141 (25%), Positives = 63/141 (44%), Gaps = 5/141 (3%)
Frame = +1
Query: 163 NLQCLLGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEY 342
N ++GI+C D +++ ++ + S M++ +I+ + M V G + D Q
Sbjct: 33 NSGTVVGIKCKDGIVMGVEKLIA-SKMMLPGSNRRIHSVHRHAGMAVAGLAADGRQIVAR 91
Query: 343 IAKNIXLYKMRNG-----YELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPE 507
Y+ G EL A+ L +LR P+ V++GGYD++ GP+
Sbjct: 92 AKSEARSYESVYGDAVPVKELSERVASYVHLCTLYWWLR---PFGCGVILGGYDRD-GPQ 147
Query: 508 LYFMDYLXSSVKVPFAAHGYG 570
LY ++ S + AA G G
Sbjct: 148 LYMIEPSGISYRYFGAAIGKG 168
>At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1)
(PRCT) almost identical to GB:AAC32069 from [Arabidopsis
thaliana], EST gb|T76747 comes from this gene; identical
to cDNA proteasome subunit prct GI:2511567
Length = 204
Score = 38.7 bits (86), Expect = 0.003
Identities = 26/105 (24%), Positives = 47/105 (44%)
Frame = +1
Query: 208 IAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIXLYKMRNGYE 387
IA+D+ + + D +I KI DR+ +G+ G + D + + LY++R +
Sbjct: 22 IASDRRLGVQLQTIATDFQRISKIHDRVFIGLSGLATDVQTLYQRLVFRHKLYQLREERD 81
Query: 388 LGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMD 522
+ P AS L Y + PY ++ G ++ P + MD
Sbjct: 82 MKPETFASLVSAIL--YEKRFGPYLCQPVIAGLGDDDKPFICTMD 124
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 37.1 bits (82), Expect = 0.009
Identities = 27/107 (25%), Positives = 46/107 (42%)
Frame = +1
Query: 202 VMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIXLYKMRNG 381
VM+AAD S I KI +I+ ++ + G + D + + L+++ N
Sbjct: 69 VMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANK 128
Query: 382 YELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMD 522
+ S A+ L Y V ++ G+D E GP LY++D
Sbjct: 129 RRISVSGASKLLANMLYSY--RGMGLSVGTMIAGWD-ETGPGLYYVD 172
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 37.1 bits (82), Expect = 0.009
Identities = 27/107 (25%), Positives = 46/107 (42%)
Frame = +1
Query: 202 VMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIXLYKMRNG 381
VM+AAD S I KI +I+ ++ + G + D + + L+++ N
Sbjct: 69 VMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANK 128
Query: 382 YELGPSAAASFTRRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMD 522
+ S A+ L Y V ++ G+D E GP LY++D
Sbjct: 129 RRISVSGASKLLANMLYSY--RGMGLSVGTMIAGWD-ETGPGLYYVD 172
>At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1)
(PRC9) identical to GB:AAC32057 from [Arabidopsis
thaliana] (Genetics (1998) 149 (2), 677-692); identical
to cDNA proteasome subunit prc9 GI:2511583
Length = 250
Score = 34.3 bits (75), Expect = 0.060
Identities = 26/123 (21%), Positives = 46/123 (37%), Gaps = 3/123 (2%)
Frame = +1
Query: 163 NLQCLLGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEY 342
N +GI D V++ ++ + ++ K+YKI D + V G D N
Sbjct: 30 NAGSAIGILSKDGVVLIGEKKVTSKLLQTSTSAEKMYKIDDHVACAVAGIMSDANILINT 89
Query: 343 IAKNIXLYKMRNGYELGPSAAASF---TRRNLAEYLRSSTPYFVNVLMGGYDKENGPELY 513
Y + T++ ++ P+ V+ L G+DK +G +LY
Sbjct: 90 ARVQAQRYTFMYQEPMPVEQLVQSLCDTKQGYTQF-GGLRPFGVSFLFAGWDKHHGFQLY 148
Query: 514 FMD 522
D
Sbjct: 149 MSD 151
>At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2)
identical to 20S proteasome subunit PAE2 GB:AAC32061
from [Arabidopsis thaliana]
Length = 237
Score = 33.9 bits (74), Expect = 0.079
Identities = 29/120 (24%), Positives = 52/120 (43%), Gaps = 5/120 (4%)
Frame = +1
Query: 178 LGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 357
+G++ + V++A ++ + ++ E KI +I D + + G D E+
Sbjct: 38 IGVKTKEGVVLAVEKRITSPLLEPSSVE-KIMEIDDHIGCAMSGLIADARTLVEHARVET 96
Query: 358 XLYKMRNG----YELGPSAAASFTRR-NLAEYLRSSTPYFVNVLMGGYDKENGPELYFMD 522
++ G E A R E S P+ V++L+ G+D ENGP LY+ D
Sbjct: 97 QNHRFSYGEPMTVESTTQALCDLALRFGEGEEESMSRPFGVSLLIAGHD-ENGPSLYYTD 155
>At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1)
identical to 20S proteasome subunit PAE1 GI:3421087 from
[Arabidopsis thaliana]
Length = 237
Score = 33.9 bits (74), Expect = 0.079
Identities = 29/120 (24%), Positives = 52/120 (43%), Gaps = 5/120 (4%)
Frame = +1
Query: 178 LGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 357
+G++ + V++A ++ + ++ E KI +I D + + G D E+
Sbjct: 38 IGVKTKEGVVLAVEKRITSPLLEPSSVE-KIMEIDDHIGCAMSGLIADARTLVEHARVET 96
Query: 358 XLYKMRNG----YELGPSAAASFTRR-NLAEYLRSSTPYFVNVLMGGYDKENGPELYFMD 522
++ G E A R E S P+ V++L+ G+D ENGP LY+ D
Sbjct: 97 QNHRFSYGEPMTVESTTQALCDLALRFGEGEEESMSRPFGVSLLIAGHD-ENGPSLYYTD 155
>At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1)
(PRC1) identical to proteasome subunit alpha type 6-1
SP:O81146 GI:12643647 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc1 GI:2511587
Length = 246
Score = 30.7 bits (66), Expect = 0.74
Identities = 23/117 (19%), Positives = 46/117 (39%), Gaps = 2/117 (1%)
Frame = +1
Query: 178 LGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 357
+G++ D V + Q ++ + ++ I+ + + G + D +
Sbjct: 40 IGVRGKDSVCVVT-QKKVPDKLLDQSSVTHLFPITKYIGLVATGITADARSLVQQARNQA 98
Query: 358 XLYKMRNGYELGPSAAASFTRRNLAEYLRSS--TPYFVNVLMGGYDKENGPELYFMD 522
++ GYE+ A + Y + + P V ++ G D+ENGP LY D
Sbjct: 99 AEFRFTYGYEMPVDILAKWIADKSQVYTQHAYMRPLGVVAMVMGVDEENGPLLYKCD 155
>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
identical to GB:AF043519
Length = 246
Score = 29.5 bits (63), Expect = 1.7
Identities = 22/117 (18%), Positives = 47/117 (40%), Gaps = 2/117 (1%)
Frame = +1
Query: 178 LGIQCNDFVMIAADQSNSHSIMIMKDDEXKIYKISDRLVMGVIGDSGDTNQFAEYIAKNI 357
+G++ D V + Q ++ + ++ ++ L + G + D+ +
Sbjct: 40 IGVRGKDSVCVVT-QKKVPDKLLDQSSVSHLFPVTKYLGLLATGMTADSRSLVQQARNEA 98
Query: 358 XLYKMRNGYELGPSAAASFTRRNLAEYLRSS--TPYFVNVLMGGYDKENGPELYFMD 522
++ + GYE+ A + Y + + P V ++ G D+E GP LY D
Sbjct: 99 AEFRFQYGYEMPADILAKWIADKSQVYTQHAYMRPLGVVAMVLGIDEERGPLLYKCD 155
>At1g48840.1 68414.m05468 expressed protein contains Pfam profile:
PF04842 plant protein of unknown function (DUF639)
Length = 691
Score = 28.3 bits (60), Expect = 4.0
Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Frame = +2
Query: 380 GMNWGPQLQPVSHVVISQ--SILGAVLPTL 463
G NW P+L PV++VVI + ILG + L
Sbjct: 42 GANWIPELSPVANVVIRRCSKILGVAVSEL 71
>At1g19030.1 68414.m02369 hypothetical protein
Length = 398
Score = 27.1 bits (57), Expect = 9.1
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +3
Query: 519 GLFXIKREGTICSSWLW 569
G F + +E T C SW+W
Sbjct: 143 GSFWLVKENTTCGSWIW 159
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,170,759
Number of Sequences: 28952
Number of extensions: 206153
Number of successful extensions: 401
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 390
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 398
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1141585696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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