Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P04_F_C20
         (651 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g59180.1 68418.m07417 DNA-directed RNA polymerase II identica...   101   5e-22
At3g22900.1 68416.m02886 RNA polymerase Rpb7 N-terminal domain-c...    51   8e-07
At4g14660.1 68417.m02254 RNA polymerase Rpb7 N-terminal domain-c...    50   2e-06
At1g06790.2 68414.m00722 RNA polymerase Rpb7 N-terminal domain-c...    30   1.2  
At1g06790.1 68414.m00723 RNA polymerase Rpb7 N-terminal domain-c...    30   1.2  
At2g44800.1 68415.m05575 oxidoreductase, 2OG-Fe(II) oxygenase fa...    28   4.7  

>At5g59180.1 68418.m07417 DNA-directed RNA polymerase II identical
           to Swiss-Prot:P38421 DNA-directed RNA polymerase II 19
           kDa polypeptide (EC 2.7.7.6) (RNA polymerase II subunit
           5) [Arabidopsis thaliana]
          Length = 176

 Score =  101 bits (241), Expect = 5e-22
 Identities = 48/93 (51%), Positives = 64/93 (68%)
 Frame = +3

Query: 321 KYKAVVFRPFKGEVLXGIVTQVNKVGMFAQIGPLKCFISHHSIPXYMEFCPNVNPPCYKS 500
           KY+ VVFRPFKGE+L  +VT VNK+G FA+ GP++ F+S H IP  MEF    + P Y +
Sbjct: 71  KYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEFQAG-DMPNYTT 129

Query: 501 KQEDXVIQEEDVIRLKIVGTRVDXSGIFAIGTL 599
                 IQ+E  +RLKI+GTRVD + IF +GT+
Sbjct: 130 SDGSVKIQKECEVRLKIIGTRVDATAIFCVGTI 162



 Score = 91.5 bits (217), Expect = 4e-19
 Identities = 39/69 (56%), Positives = 51/69 (73%)
 Frame = +2

Query: 110 MFYHISLEHEILLHPKYFGPQLLETVKQKLYTDVEGTCTGKYGFVIAVTQIDNIGAGLIQ 289
           MF+HI LE  + LHP++FG  L E +  KL  DVEGTC+G++GFV+A+T ID IG GLI+
Sbjct: 1   MFFHIVLERNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIGKGLIR 60

Query: 290 HGQGFVMYP 316
            G GFV +P
Sbjct: 61  DGTGFVTFP 69


>At3g22900.1 68416.m02886 RNA polymerase Rpb7 N-terminal
           domain-containing protein weak similarity to SP|P52433
           DNA-directed RNA polymerase II 19 kDa polypeptide (EC
           2.7.7.6) (RPB7) {Rattus norvegicus}; contains Pfam
           profile PF03876: RNA polymerase Rpb7, N-terminal domain
          Length = 174

 Score = 50.8 bits (116), Expect = 8e-07
 Identities = 22/55 (40%), Positives = 36/55 (65%), Gaps = 1/55 (1%)
 Frame = +3

Query: 324 YKAVVFRPFKGEVLXGIVTQVNKVGMFAQIGPLK-CFISHHSIPXYMEFCPNVNP 485
           +  + F+ FKGE++ G+V +V+K G+F + GP +  ++SH  +P Y EF P  NP
Sbjct: 75  FNGICFKMFKGEIVHGVVHKVHKTGVFLKSGPYEIIYLSHMKMPGY-EFIPGENP 128


>At4g14660.1 68417.m02254 RNA polymerase Rpb7 N-terminal
           domain-containing protein similar to SP|P52433
           DNA-directed RNA polymerase II 19 kDa polypeptide (EC
           2.7.7.6) (RPB7) {Rattus norvegicus}; contains Pfam
           profile PF03876: RNA polymerase Rpb7, N-terminal domain
          Length = 178

 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 25/81 (30%), Positives = 47/81 (58%), Gaps = 1/81 (1%)
 Frame = +3

Query: 324 YKAVVFRPFKGEVLXGIVTQVNKVGMFAQIGPLK-CFISHHSIPXYMEFCPNVNPPCYKS 500
           +  + F+ FKGE++ G+V +V K G+F + GP++  ++S+  +P Y ++ P  NP     
Sbjct: 76  FSGMTFKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTKMPDY-KYIPGENPIFMNE 134

Query: 501 KQEDXVIQEEDVIRLKIVGTR 563
           K     IQ E  +R+ ++G +
Sbjct: 135 KTSR--IQVETTVRVVVIGIK 153



 Score = 31.5 bits (68), Expect = 0.50
 Identities = 13/31 (41%), Positives = 20/31 (64%)
 Frame = +2

Query: 224 TGKYGFVIAVTQIDNIGAGLIQHGQGFVMYP 316
           T + G+ +AVT +D IG G I+   G V++P
Sbjct: 43  TKELGYYVAVTTLDKIGEGKIREHTGEVLFP 73


>At1g06790.2 68414.m00722 RNA polymerase Rpb7 N-terminal
           domain-containing protein similar to SP|P35718
           DNA-directed RNA polymerase III 25 kDa polypeptide (EC
           2.7.7.6) {Saccharomyces cerevisiae}; contains Pfam
           profile PF03876: RNA polymerase Rpb7, N-terminal domain
          Length = 189

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 16/68 (23%), Positives = 26/68 (38%)
 Frame = +2

Query: 110 MFYHISLEHEILLHPKYFGPQLLETVKQKLYTDVEGTCTGKYGFVIAVTQIDNIGAGLIQ 289
           MFY   LEH + + P      L + +K  L            G  +++  I ++  G + 
Sbjct: 1   MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60

Query: 290 HGQGFVMY 313
            G G   Y
Sbjct: 61  PGDGAATY 68


>At1g06790.1 68414.m00723 RNA polymerase Rpb7 N-terminal
           domain-containing protein similar to SP|P35718
           DNA-directed RNA polymerase III 25 kDa polypeptide (EC
           2.7.7.6) {Saccharomyces cerevisiae}; contains Pfam
           profile PF03876: RNA polymerase Rpb7, N-terminal domain
          Length = 204

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 16/68 (23%), Positives = 26/68 (38%)
 Frame = +2

Query: 110 MFYHISLEHEILLHPKYFGPQLLETVKQKLYTDVEGTCTGKYGFVIAVTQIDNIGAGLIQ 289
           MFY   LEH + + P      L + +K  L            G  +++  I ++  G + 
Sbjct: 1   MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60

Query: 290 HGQGFVMY 313
            G G   Y
Sbjct: 61  PGDGAATY 68


>At2g44800.1 68415.m05575 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein similar to flavonol synthase SP|Q96330
           {Arabidopsis thaliana}, SP|Q07512 {Petunia hybrida};
           contains Pfam profile PF03171: oxidoreductase,
           2OG-Fe(II) oxygenase family
          Length = 357

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
 Frame = +3

Query: 429 FISHHSIPX--YMEFCPNVNPPCYKSK 503
           FI H+S P   +++  P+ NPPCYK K
Sbjct: 144 FIKHYSHPLSKWIDMWPS-NPPCYKDK 169


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,815,744
Number of Sequences: 28952
Number of extensions: 283055
Number of successful extensions: 667
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 650
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 666
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)