BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_B18
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC637.07 |moe1||translation initiation factor eIF3d Moe1|Schiz... 136 3e-33
SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces p... 27 2.4
SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosa... 26 4.1
SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr 2... 26 4.1
SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 25 7.2
SPAC3A12.04c |||RNase P and RNase MRP subunit p30 |Schizosacchar... 25 9.5
SPCP31B10.02 |||conserved eukaryotic protein|Schizosaccharomyces... 25 9.5
>SPAC637.07 |moe1||translation initiation factor eIF3d
Moe1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 567
Score = 136 bits (328), Expect = 3e-33
Identities = 76/210 (36%), Positives = 113/210 (53%), Gaps = 3/210 (1%)
Frame = +1
Query: 22 WGQRGAPMKIRDASVTVRPTWVTIEDMDFPRSCQTIITWLLKKVKILSVAVLXXXXXXXX 201
W ++R+ASVTV W +++++F + + V + S +
Sbjct: 163 WKDYDKHQRLRNASVTVGDDWQLLDEVEFSHLSKLNLA-AAAPVTVDSYGYIYPYDKSFD 221
Query: 202 XXXXXXXXXPCQRIXRIFHTVTTTDDPVIRRLSKTA-GTVYATDAILATIMCCTRSNYSW 378
P Q + R+ + TTT+DPVI++L+ + ++ TD+IL+ +MC TRS Y W
Sbjct: 222 KIHVKSEK-PLQALDRVHYNPTTTEDPVIQKLALNSDANIFITDSILSLLMCSTRSVYPW 280
Query: 379 DIVIEKIGDKLFLDKRDNTEFDLLTVNETSVEPP--ADDGNSINSPRNLALEATFINHNF 552
DIVI KLF DKR+ FD LTVNE + + P AD+ +NSP L++EAT+IN NF
Sbjct: 281 DIVITHQSGKLFFDKREGGPFDYLTVNENAYDSPMDADNREGVNSPGALSVEATYINQNF 340
Query: 553 SQQVLKSGPTEPKYKFQEPNPFVSEEEDGE 642
Q L+ E KYK PNPF + +E+ E
Sbjct: 341 CVQALRE-TEEEKYKLPHPNPFYNSKEESE 369
Score = 31.9 bits (69), Expect = 0.083
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = +3
Query: 165 SCGTLEYYDKAYDRVNVKHEKPLPA 239
S G + YDK++D+++VK EKPL A
Sbjct: 209 SYGYIYPYDKSFDKIHVKSEKPLQA 233
>SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 536
Score = 27.1 bits (57), Expect = 2.4
Identities = 15/40 (37%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = +3
Query: 120 PNYHYLAIKEGEDIVSCGTLEYYDK-AYDRVNVKHEKPLP 236
P Y LA++E ++ CG Y DK DRV K +P
Sbjct: 459 PQYSPLAVEEQVPLIYCGVKGYLDKIPVDRVVEFEHKFIP 498
>SPBC244.01c |sid4||SIN component scaffold protein Sid4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 660
Score = 26.2 bits (55), Expect = 4.1
Identities = 17/71 (23%), Positives = 30/71 (42%)
Frame = +1
Query: 361 RSNYSWDIVIEKIGDKLFLDKRDNTEFDLLTVNETSVEPPADDGNSINSPRNLALEATFI 540
R N S + + ++F + T+ L +V PP N N N +L + +
Sbjct: 180 RDNISVPNQQDGLDPEVFNQQSKETKKSLQVPPSRNVPPPVTRPNQYNPEPNFSLSSGYP 239
Query: 541 NHNFSQQVLKS 573
+FSQ L++
Sbjct: 240 QQHFSQPELQN 250
>SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 497
Score = 26.2 bits (55), Expect = 4.1
Identities = 7/17 (41%), Positives = 12/17 (70%)
Frame = -3
Query: 108 EVHIFNRDPCWPYSYRC 58
+VH +++ WPY+Y C
Sbjct: 375 DVHSYSQSVLWPYAYTC 391
>SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1052
Score = 25.4 bits (53), Expect = 7.2
Identities = 16/53 (30%), Positives = 24/53 (45%)
Frame = +1
Query: 430 NTEFDLLTVNETSVEPPADDGNSINSPRNLALEATFINHNFSQQVLKSGPTEP 588
N EF+ T + S + GNS+++P N E + S VL G +P
Sbjct: 642 NEEFENSTSTDGSETNKNEQGNSLSTPDN---EPRLRKESLSSDVLLDGSEKP 691
>SPAC3A12.04c |||RNase P and RNase MRP subunit p30
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 235
Score = 25.0 bits (52), Expect = 9.5
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 382 IVIEKIGDKLFLDKRDNTEFDLLTVNETSVEP 477
+ I IGD+L + EFD+L+++ T P
Sbjct: 91 LAIRPIGDRLLQQTCSDLEFDILSIDFTQRLP 122
>SPCP31B10.02 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 143
Score = 25.0 bits (52), Expect = 9.5
Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = +3
Query: 120 PNYHYLAIKEGEDIVSCGTLEYYDKAYD--RVNVKHEKPL 233
PN +LA++EG + ++ + YD RV K E+PL
Sbjct: 23 PNVSFLAVEEGPASLRSYSIGNVSRIYDGIRVPPKPEEPL 62
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,557,444
Number of Sequences: 5004
Number of extensions: 51369
Number of successful extensions: 154
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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