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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P04_F_B18
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g20980.1 68417.m03037 eukaryotic translation initiation facto...   146   1e-35
At5g44320.1 68418.m05427 eukaryotic translation initiation facto...   137   7e-33
At2g22620.1 68415.m02681 expressed protein                             29   2.0  
At5g19870.1 68418.m02363 expressed protein contains Pfam profile...    28   6.2  
At5g13300.1 68418.m01528 ARF GTPase-activating domain-containing...    28   6.2  
At4g36870.1 68417.m05228 BEL1-like homeobox 2 protein (BLH2)           27   8.2  

>At4g20980.1 68417.m03037 eukaryotic translation initiation factor 3
           subunit 7, putative / eIF-3 zeta, putative / eIF3d,
           putative similar to initiation factor 3d [Arabidopsis
           thaliana] GI:12407755, SP|O15371 Eukaryotic translation
           initiation factor 3 subunit 7 (eIF-3 zeta) (eIF3 p66)
           (eIF3d) {Homo sapiens}; contains Pfam profile PF05091:
           Eukaryotic translation initiation factor 3 subunit 7
           (eIF-3)
          Length = 591

 Score =  146 bits (354), Expect = 1e-35
 Identities = 78/137 (56%), Positives = 98/137 (71%), Gaps = 2/137 (1%)
 Frame = +1

Query: 247 RIFHTVTTTDDPVIRRLSKT-AGTVYATDAILATIMCCTRSNYSWDIVIEKIGDKLFLDK 423
           R F  VTT+DDPVIRRL+K    TV+ATDAILA +MC  RS YSWDIVI+++G+KLF DK
Sbjct: 245 RNFFKVTTSDDPVIRRLAKEDKATVFATDAILAALMCAPRSVYSWDIVIQRVGNKLFFDK 304

Query: 424 RDNTEFDLLTVNETSVEPPADDGNSINSPRNLALEATFINHNFSQQVL-KSGPTEPKYKF 600
           RD ++ DLL+V+ETS EP  +  + INS  +L +EA +IN NFSQQVL + G  E    F
Sbjct: 305 RDGSQLDLLSVHETSQEPLPESKDDINSAHSLGVEAAYINQNFSQQVLVRDGKKE---TF 361

Query: 601 QEPNPFVSEEEDGEVAS 651
            E NPF +E E  E+AS
Sbjct: 362 DEANPFANEGE--EIAS 376



 Score = 39.5 bits (88), Expect = 0.002
 Identities = 14/34 (41%), Positives = 25/34 (73%)
 Frame = +3

Query: 141 IKEGEDIVSCGTLEYYDKAYDRVNVKHEKPLPAY 242
           ++E ED++ CG LEYY++ +DR+  K+E+ L  +
Sbjct: 209 VQEPEDLLLCGGLEYYNRLFDRITPKNERRLERF 242


>At5g44320.1 68418.m05427 eukaryotic translation initiation factor 3
           subunit 7, putative / eIF-3 zeta, putative / eIF3d,
           putative similar to initiation factor 3d [Arabidopsis
           thaliana] GI:12407755, SP|O15371 Eukaryotic translation
           initiation factor 3 subunit 7 (eIF-3 zeta) (eIF3 p66)
           (eIF3d) {Homo sapiens}; contains Pfam profile PF05091:
           Eukaryotic translation initiation factor 3 subunit 7
           (eIF-3)
          Length = 588

 Score =  137 bits (331), Expect = 7e-33
 Identities = 74/132 (56%), Positives = 95/132 (71%), Gaps = 3/132 (2%)
 Frame = +1

Query: 262 VTTTDDPVIRRLSKT-AGTVYATDAILATIMCCTRSNYSWDIVIEKIGDKLFLDKRDNTE 438
           VTT+DD VIRRL+K    TV+ATDAILA +MC  RS YSWD+VI+++G+KLF DKRD + 
Sbjct: 245 VTTSDDLVIRRLAKEDKATVFATDAILAALMCAPRSVYSWDLVIQRVGNKLFFDKRDGSP 304

Query: 439 FDLLTVNETSVEPPADDGNSINSPRNLALEATFINHNFSQQVL-KSGPTEPKYKFQEPNP 615
            DLL+V+ETS EP  +  + INS  +L LEA +IN NF+QQVL K+G  E    F EP P
Sbjct: 305 LDLLSVHETSQEPLPEGKDDINSAHSLGLEAAYINQNFAQQVLVKNGKRE---TFDEPIP 361

Query: 616 FVSE-EEDGEVA 648
            V+E EE+  +A
Sbjct: 362 NVNEGEENASIA 373



 Score = 35.9 bits (79), Expect = 0.024
 Identities = 13/34 (38%), Positives = 23/34 (67%)
 Frame = +3

Query: 141 IKEGEDIVSCGTLEYYDKAYDRVNVKHEKPLPAY 242
           + E ED++ CG LE YD+++DR+  K ++ L  +
Sbjct: 205 VSEPEDLLLCGGLESYDRSFDRITPKADRRLERF 238


>At2g22620.1 68415.m02681 expressed protein
          Length = 677

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 12/33 (36%), Positives = 20/33 (60%)
 Frame = +1

Query: 376 WDIVIEKIGDKLFLDKRDNTEFDLLTVNETSVE 474
           WD+V  +   K   DK + T+F+++T NE  +E
Sbjct: 102 WDVVWYEPEKKQKTDKLEGTKFEIITQNEEQIE 134


>At5g19870.1 68418.m02363 expressed protein contains Pfam profile
           PF04819: Family of unknown function (DUF716) (Plant
           viral-response family)
          Length = 276

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 11/37 (29%), Positives = 22/37 (59%)
 Frame = +3

Query: 108 PTLLPNYHYLAIKEGEDIVSCGTLEYYDKAYDRVNVK 218
           P+L+P   +L I+EG+  + C  ++   +A   VN++
Sbjct: 204 PSLVPKDCFLHIEEGKHTIRCSDVKALHRAISLVNIQ 240


>At5g13300.1 68418.m01528 ARF GTPase-activating domain-containing
           protein similar to GCN4-complementing protein (GCP1)
           GI:6465806 from [Arabidopsis thaliana]
          Length = 768

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
 Frame = -1

Query: 143 NSQVMIVWQERGKSISSIVTHVGLTVTDASRIFI-GAP 33
           N+    VW+E   S S+I    GLTV+D SR+ + G P
Sbjct: 517 NTFANTVWEELLHSRSAIHFDPGLTVSDKSRVMVTGKP 554


>At4g36870.1 68417.m05228 BEL1-like homeobox 2 protein (BLH2)
          Length = 638

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = -1

Query: 539 IKVASSARFLGELIEFPSSAGGSTEVSL 456
           I V  SA  L E+  FP S+GG  ++ L
Sbjct: 78  IPVYESAGMLSEMFNFPGSSGGGRDLDL 105


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,527,934
Number of Sequences: 28952
Number of extensions: 270900
Number of successful extensions: 725
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 708
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 723
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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