BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P04_F_B12 (655 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 29 0.59 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 4.1 SPAC3A11.13 ||SPAC3H5.02|prefoldin subunit 6|Schizosaccharomyces... 26 5.5 SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 25 7.2 SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|c... 25 9.5 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 25 9.5 >SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1055 Score = 29.1 bits (62), Expect = 0.59 Identities = 14/54 (25%), Positives = 27/54 (50%) Frame = +1 Query: 436 DQLQDIQSVLPDMTDFKYIGTETMQDADTAKWQMVQPVGDKLNKYTMWVKYXKT 597 +++ I S+L + + Y + + +AD A+ QP GD L +W ++ T Sbjct: 857 EEVLSIVSMLGEASSLFYRPKDKIMEADKARANFTQPGGDHLTLLHIWNEWVDT 910 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 26.2 bits (55), Expect = 4.1 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = -3 Query: 590 LYLTHIVYLFSLSPTGCT 537 +Y H+VY+ SLSP C+ Sbjct: 1399 VYAAHLVYILSLSPDVCS 1416 >SPAC3A11.13 ||SPAC3H5.02|prefoldin subunit 6|Schizosaccharomyces pombe|chr 1|||Manual Length = 114 Score = 25.8 bits (54), Expect = 5.5 Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 1/48 (2%) Frame = +1 Query: 388 ETEMNXETCLQVNSTQDQLQDIQSVLPDMTDFKYIG-TETMQDADTAK 528 E+ ET LQ N+T L +++ V PD +K IG T Q + AK Sbjct: 20 ESLKKLETQLQENTTV--LNELEKVAPDSNIYKQIGPTLVKQSHEEAK 65 >SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr 3|||Manual Length = 1024 Score = 25.4 bits (53), Expect = 7.2 Identities = 15/48 (31%), Positives = 23/48 (47%) Frame = +3 Query: 306 RYGQDLPVHVSGLPTLRYFYKDRSGHNGNRDEQXDVPASQFDARSAPR 449 +Y D+P +VS LP R Y + G DE+ + D S+P+ Sbjct: 796 QYHVDMPDNVSDLPQTRLCYGACVYNVGLLDEEKRKDLANSDLNSSPK 843 >SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/45 (28%), Positives = 22/45 (48%) Frame = +1 Query: 433 QDQLQDIQSVLPDMTDFKYIGTETMQDADTAKWQMVQPVGDKLNK 567 QD+L+D+ + P + K GT+ D ++W V D L + Sbjct: 479 QDKLRDLAAKHPYFEEVKRYGTDANGDPLWSEWFYNPDVDDDLER 523 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 25.0 bits (52), Expect = 9.5 Identities = 9/12 (75%), Positives = 10/12 (83%) Frame = +1 Query: 187 QWSPVYTVKGLL 222 QWSPVYT+K L Sbjct: 96 QWSPVYTMKSAL 107 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,717,295 Number of Sequences: 5004 Number of extensions: 55970 Number of successful extensions: 139 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 139 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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