Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_B06
(655 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 142 4e-35
SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|c... 33 0.036
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 29 0.44
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 29 0.77
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 29 0.77
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 27 1.8
SPAC869.06c |||cation binding protein |Schizosaccharomyces pombe... 27 3.1
SPAC24C9.09 |||mitochondrial threonine-tRNA ligase|Schizosacchar... 26 5.5
SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 26 5.5
SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 25 7.2
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 25 7.2
SPAC222.06 |mak16||nuclear HMG-like acidic protein Mak16|Schizos... 25 9.5
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 142 bits (344), Expect = 4e-35
Identities = 75/190 (39%), Positives = 114/190 (60%), Gaps = 3/190 (1%)
Frame = +3
Query: 93 KFDLTFKIGQYLDRHLVFPLLEFLAAKETYDQSELLQAKLEILSKTNMIDYVTDIRRML- 269
K+DL+ KI Q+LDRHL+FPLLEFL+ ++T+D ELLQAK ++L TNM DYV ++ L
Sbjct: 14 KYDLSQKIMQHLDRHLIFPLLEFLSLRQTHDPKELLQAKYDLLKDTNMTDYVANLWTNLH 73
Query: 270 --YPEEDTPEXIIQRRGVVLSELQELQDAVXPVLRLMQRDDVMKTIETMRDPKTLINHLS 443
+ +ED ++R VL EL EL++ V +L +++ D++ + +D + HL
Sbjct: 74 GGHTDEDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALR--QDKGQNLQHL- 130
Query: 444 TNKEYEFKIEMIDSMYRLAKYRYECGNYVESASYLYFCQLVMSPTDKNYLSVLWGKLASE 623
+ Y E I +Y+ A+++Y CGNY ++ LY + + N S WGK ASE
Sbjct: 131 -QEHYNITPERIAVLYKFAQFQYNCGNYGGASDLLYHFRAFSKDPELN-ASATWGKFASE 188
Query: 624 ILVQNWDGAL 653
IL +WDGA+
Sbjct: 189 ILTVDWDGAM 198
>SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 796
Score = 33.1 bits (72), Expect = 0.036
Identities = 22/61 (36%), Positives = 31/61 (50%)
Frame = -3
Query: 593 QIILVSWRHHQLTKVKI*C*LHIISTFIAIFGQTIHAIDHFNFKLILLVCRKMVYKSFRI 414
Q++ V W+HHQ C I + F IFGQ I AID ++ KL + + +SF
Sbjct: 332 QLLEVPWQHHQ-------CHPLIKTHFFGIFGQKIDAIDFYSAKLYKISQQIENARSFDY 384
Query: 413 P 411
P
Sbjct: 385 P 385
>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 2410
Score = 29.5 bits (63), Expect = 0.44
Identities = 11/44 (25%), Positives = 25/44 (56%)
Frame = +3
Query: 396 TIETMRDPKTLINHLSTNKEYEFKIEMIDSMYRLAKYRYECGNY 527
T+ T+ D ++ HL+ +F + ++M++ ++YRY N+
Sbjct: 180 TVATLGDLIGMVGHLNDTSGVDFNLNEYNAMWKTSEYRYVDFNF 223
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 28.7 bits (61), Expect = 0.77
Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +3
Query: 108 FKIGQYLDRHLVFPLLEF-LAAKETYDQSELLQAKLEILSK 227
F I +YL +L+ P LEF L A E Q + A ++ SK
Sbjct: 1218 FHISKYLSSNLLLPFLEFLLTASEAEAQIPVTDASQKVSSK 1258
>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 793
Score = 28.7 bits (61), Expect = 0.77
Identities = 14/59 (23%), Positives = 29/59 (49%), Gaps = 5/59 (8%)
Frame = -3
Query: 518 TFIAIFGQTIHAIDHFNFKLILLVCRKMVYKSFRIPH-----GFNCFHYIISLHESQYW 357
TF+ +G I+ D +N L +VY ++ + F+ + ++S+++SQ W
Sbjct: 83 TFLVKYGDLINIQDPYNLNKTLFSVSDLVYNGIQLDYDSYSISFDAKYVLVSVNKSQRW 141
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 27.5 bits (58), Expect = 1.8
Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 3/44 (6%)
Frame = +3
Query: 381 DDVMKTIETMRDPKTLINHLSTNKE---YEFKIEMIDSMYRLAK 503
+DV+K + T+ PK +I L ++ +EF I+ ID ++ +K
Sbjct: 3764 NDVLKKLITILSPKVIITKLDFERDTFVHEFGIDSIDLIWLASK 3807
>SPAC869.06c |||cation binding protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 203
Score = 26.6 bits (56), Expect = 3.1
Identities = 15/38 (39%), Positives = 18/38 (47%), Gaps = 1/38 (2%)
Frame = -2
Query: 648 RHPNFVPKFR*PIYP-KEPTNNSCQLATSPVDKSKDMM 538
R FVP P P K P L +P+DK +DMM
Sbjct: 162 RTKKFVPTHSHPSAPNKPPFETVAGLFAAPIDKLRDMM 199
>SPAC24C9.09 |||mitochondrial threonine-tRNA
ligase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 473
Score = 25.8 bits (54), Expect = 5.5
Identities = 16/64 (25%), Positives = 32/64 (50%), Gaps = 5/64 (7%)
Frame = -3
Query: 422 FRIPHGFNCFHYIISLHESQYWLYC---ILKFLQFRKNY--TSPLYNXFRSVFFRIEHSS 258
F +PHG ++ ++ +QY ++ I+ L F+K+ S + + FR+E S
Sbjct: 69 FFLPHGTRIYNRLVDFLRAQYQIHGFEEIITPLIFKKDLWEKSGHWQNYEKEIFRVEESR 128
Query: 257 DVSN 246
+V +
Sbjct: 129 NVED 132
>SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 781
Score = 25.8 bits (54), Expect = 5.5
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = -2
Query: 582 CQLATSPVDKSKDMMLTPHNFHIHSDIW 499
C++ V KS D+ + H F ++SD W
Sbjct: 506 CRMEHPNVVKSYDLCIDSHIFPLYSDTW 533
>SPCC553.09c |spb70|pol12|DNA polymerase alpha
B-subunit|Schizosaccharomyces pombe|chr 3|||Manual
Length = 574
Score = 25.4 bits (53), Expect = 7.2
Identities = 12/44 (27%), Positives = 21/44 (47%)
Frame = +3
Query: 171 KETYDQSELLQAKLEILSKTNMIDYVTDIRRMLYPEEDTPEXII 302
++ YD SE+L ++E+ S Y + P E T E ++
Sbjct: 163 QKLYDSSEVLDDRIELFSALTCRKYNISDEDLANPSELTQEPVV 206
>SPBC30D10.10c |tor1||phosphatidylinositol kinase
Tor1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2335
Score = 25.4 bits (53), Expect = 7.2
Identities = 10/39 (25%), Positives = 21/39 (53%)
Frame = +3
Query: 357 PVLRLMQRDDVMKTIETMRDPKTLINHLSTNKEYEFKIE 473
PV+R+ + I + + P+ L H S K+Y++ ++
Sbjct: 1948 PVIRISHFHHTFEVISSKQRPRRLTIHGSDGKDYQYVLK 1986
>SPAC222.06 |mak16||nuclear HMG-like acidic protein
Mak16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 302
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = +3
Query: 390 MKTIETMRDPKTLINHLSTNKEYEFKIEMID 482
MKTIE P L + +K Y +E ID
Sbjct: 64 MKTIERAHFPSKLWQRIKLSKNYAKALEQID 94
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,631,021
Number of Sequences: 5004
Number of extensions: 51675
Number of successful extensions: 145
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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