Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_P23
(650 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 40 3e-04
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 38 0.002
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 38 0.002
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 36 0.005
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 35 0.012
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 33 0.047
SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 32 0.062
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 31 0.14
SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizo... 30 0.25
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 30 0.25
SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B... 30 0.33
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 29 0.58
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 29 0.77
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 28 1.0
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 28 1.3
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 28 1.3
SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 27 2.3
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 27 2.3
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 26 4.1
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 26 4.1
SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non ca... 26 4.1
SPBC776.18c |pmh1|mcr1|transcription factor TFIIH complex subuni... 26 4.1
SPAC3H1.09c |||vacuolar amino acid transporter |Schizosaccharomy... 25 9.5
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 9.5
>SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 39.9 bits (89), Expect = 3e-04
Identities = 21/78 (26%), Positives = 41/78 (52%), Gaps = 3/78 (3%)
Frame = +1
Query: 322 VNEHITCPLCXGYYIXATTIVECLHSFCRSCIIKHLKAKSYCPVCEMMINSAKPNIKLDK 501
++ + C +C Y+ A I C H+FC CI +L+ CP C ++ ++ +
Sbjct: 23 LDSSLRCLICHEYF-RAPLITSCSHTFCSFCIRDYLREHPMCPACRAPEQESR--LRKNT 79
Query: 502 ALQDIV--YKLV-PGLFQ 546
L++I+ +K++ P LF+
Sbjct: 80 ILEEILESFKVIRPTLFE 97
>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 37.5 bits (83), Expect = 0.002
Identities = 18/47 (38%), Positives = 22/47 (46%)
Frame = +1
Query: 340 CPLCXGYYIXATTIVECLHSFCRSCIIKHLKAKSYCPVCEMMINSAK 480
C LC + I EC H FC SCI KS CP+C + +K
Sbjct: 256 CSLCMEF-IHCPAATECGHIFCWSCINGWTSKKSECPLCRAFSSPSK 301
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 37.5 bits (83), Expect = 0.002
Identities = 19/75 (25%), Positives = 34/75 (45%), Gaps = 4/75 (5%)
Frame = +1
Query: 256 KKVVKMDNSNVAVVPQ-RTLLG---EVNEHITCPLCXGYYIXATTIVECLHSFCRSCIIK 423
K + ++ N +P +TLL ++ + + CP+C T C H++C C++
Sbjct: 53 KSRISSNSENKKQIPDTKTLLETFQKIKKTLECPICTEALQRPFT-THCGHTYCYECLLN 111
Query: 424 HLKAKSYCPVCEMMI 468
LK CP C +
Sbjct: 112 WLKESKSCPTCRQKL 126
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 35.9 bits (79), Expect = 0.005
Identities = 14/51 (27%), Positives = 24/51 (47%)
Frame = +1
Query: 322 VNEHITCPLCXGYYIXATTIVECLHSFCRSCIIKHLKAKSYCPVCEMMINS 474
V+ + C +C G + + C H+FC C+++ L CP C + S
Sbjct: 163 VSRELECQICFGM-LYDPVVSPCGHTFCGPCLMQALTQSPQCPTCRFGLPS 212
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 34.7 bits (76), Expect = 0.012
Identities = 16/51 (31%), Positives = 23/51 (45%)
Frame = +1
Query: 319 EVNEHITCPLCXGYYIXATTIVECLHSFCRSCIIKHLKAKSYCPVCEMMIN 471
+ H C +C I I C H +C C+ LK S CP+C+ +N
Sbjct: 1085 KAESHQICIICRDI-IKQGFITTCGHLYCSFCLEAWLKHSSSCPMCKTKLN 1134
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 32.7 bits (71), Expect = 0.047
Identities = 13/36 (36%), Positives = 22/36 (61%), Gaps = 2/36 (5%)
Frame = +1
Query: 385 ECLHSFCRSCIIKHLKAKS--YCPVCEMMINSAKPN 486
+C H++C +CI+ LK + CP+C+ I A P+
Sbjct: 212 DCGHTYCYACIMSRLKLVNNVSCPICKHRIRFALPD 247
>SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 456
Score = 32.3 bits (70), Expect = 0.062
Identities = 16/62 (25%), Positives = 28/62 (45%), Gaps = 1/62 (1%)
Frame = +1
Query: 388 CLHSFCRSCIIKHLKAK-SYCPVCEMMINSAKPNIKLDKALQDIVYKLVPGLFQKEMERR 564
C H FC C+I K K +CP+C + +D AL + + P +++ E
Sbjct: 375 CSHVFCLHCLIILQKQKVDFCPLCRAKEVMKADSRNIDHALMNFMKTYFPREIKEKFEEN 434
Query: 565 QH 570
++
Sbjct: 435 EN 436
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 31.1 bits (67), Expect = 0.14
Identities = 9/26 (34%), Positives = 17/26 (65%)
Frame = +1
Query: 379 IVECLHSFCRSCIIKHLKAKSYCPVC 456
+ C H + R C+++ ++ +S CPVC
Sbjct: 644 LTPCHHLYHRQCLLQWMETRSICPVC 669
>SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog
Rhp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 963
Score = 30.3 bits (65), Expect = 0.25
Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 5/57 (8%)
Frame = +1
Query: 301 QRTLLGEVNEHITCPLCXGYYIXATTIVECLHSFCRSCIIKHLKAKS-----YCPVC 456
++T+ E E+I C +C A C H+FCR C+ +++ A CP C
Sbjct: 698 RKTVDIENQENIVCKICDEVAQDAIES-RCHHTFCRLCVTEYINAAGDGENVNCPSC 753
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 30.3 bits (65), Expect = 0.25
Identities = 9/31 (29%), Positives = 17/31 (54%)
Frame = +1
Query: 340 CPLCXGYYIXATTIVECLHSFCRSCIIKHLK 432
CP+C I ++ C H+ C C+ +H++
Sbjct: 877 CPICCNEPIQNPLLLNCKHACCGDCLSEHIQ 907
>SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3
Brl1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 692
Score = 29.9 bits (64), Expect = 0.33
Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Frame = +1
Query: 334 ITCPLCXGYYIXATTIVECLHSFCRSCIIKHLKAK-SYCPVCE 459
+ C +C + I C H+FC +C+ + K S CP CE
Sbjct: 637 LKCSVCNFSNWKSKLIPNCGHAFCSNCMEPFYEHKTSTCPQCE 679
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 29.1 bits (62), Expect = 0.58
Identities = 13/40 (32%), Positives = 18/40 (45%), Gaps = 1/40 (2%)
Frame = +1
Query: 340 CPLCXGYYIXATTIVECLHSFCRSCIIKHLKAKS-YCPVC 456
C +C I C H FC CI K ++ + CP+C
Sbjct: 627 CSVCNFERWKDRIISLCGHGFCYQCIQKRIETRQRRCPIC 666
>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 741
Score = 28.7 bits (61), Expect = 0.77
Identities = 14/45 (31%), Positives = 22/45 (48%), Gaps = 4/45 (8%)
Frame = +1
Query: 334 ITCPLCXGYYIX----ATTIVECLHSFCRSCIIKHLKAKSYCPVC 456
+TCP+C T + C H F ++C+ K L+ CP+C
Sbjct: 105 LTCPICYDDMNENDEKQATKMPCGHIFGKNCLQKWLENHCTCPLC 149
>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 269
Score = 28.3 bits (60), Expect = 1.0
Identities = 21/80 (26%), Positives = 32/80 (40%), Gaps = 3/80 (3%)
Frame = +1
Query: 250 SDKKVVKMDNSNVAVVPQRTLLGEVNEHITCPLCXGYYIXATTIVECLHSFCRSCIIKHL 429
++ ++ K + S VVP L + C +C + C H FC CI+ L
Sbjct: 180 TNAEIEKEEPSKKQVVPSSQRLADYK----CVICLDSP-ENLSCTPCGHIFCNFCILSAL 234
Query: 430 ---KAKSYCPVCEMMINSAK 480
A CPVC ++ K
Sbjct: 235 GTTAATQKCPVCRRKVHPNK 254
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 27.9 bits (59), Expect = 1.3
Identities = 14/60 (23%), Positives = 26/60 (43%), Gaps = 2/60 (3%)
Frame = +1
Query: 298 PQRTLLGEVNEHITCPLCXGYYIXATTIVE--CLHSFCRSCIIKHLKAKSYCPVCEMMIN 471
P + L+ E E C +C + +++ C H F +CI L+ C +C ++
Sbjct: 385 PPKELIDEEGE---CTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICRAPVD 441
>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1040
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/30 (36%), Positives = 13/30 (43%)
Frame = +1
Query: 328 EHITCPLCXGYYIXATTIVECLHSFCRSCI 417
E + C LC IV C H CR C+
Sbjct: 742 ETLQCSLCMDVVAELLIIVPCGHFLCRECL 771
>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 27.1 bits (57), Expect = 2.3
Identities = 10/42 (23%), Positives = 18/42 (42%), Gaps = 3/42 (7%)
Frame = +1
Query: 340 CPLCXGYYIXA---TTIVECLHSFCRSCIIKHLKAKSYCPVC 456
CP+C Y + + C H + C ++++ CP C
Sbjct: 271 CPICGEYMFNSRERVIFLSCSHPLHQRCHEEYIRTNYRCPTC 312
>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 673
Score = 27.1 bits (57), Expect = 2.3
Identities = 10/38 (26%), Positives = 18/38 (47%)
Frame = +1
Query: 340 CPLCXGYYIXATTIVECLHSFCRSCIIKHLKAKSYCPV 453
CP C A + C H +C SC+++ ++ + V
Sbjct: 214 CPFCLEEKPVAARMSRCGHVYCFSCLLRFVETPTAAEV 251
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 26.2 bits (55), Expect = 4.1
Identities = 11/25 (44%), Positives = 14/25 (56%), Gaps = 2/25 (8%)
Frame = +1
Query: 388 CLHSFCRSCIIKHLKA--KSYCPVC 456
C H F SC+ K K+ S CP+C
Sbjct: 1579 CRHKFHASCLYKWFKSSNSSRCPLC 1603
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 26.2 bits (55), Expect = 4.1
Identities = 11/39 (28%), Positives = 18/39 (46%)
Frame = +1
Query: 322 VNEHITCPLCXGYYIXATTIVECLHSFCRSCIIKHLKAK 438
V+ TC +C EC H FC +C ++L ++
Sbjct: 125 VHHEGTCEICYDEGCLPFFSAECDHEFCLACYRQYLDSR 163
>SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non
catalytic subunit Arm1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 811
Score = 26.2 bits (55), Expect = 4.1
Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 2/32 (6%)
Frame = -3
Query: 570 MLPPFHFFLEEP--RHKFVDYVLQCFVKFDIG 481
+L HF L E VDYV +CFV F+ G
Sbjct: 342 LLLKIHFLLFESFTAESVVDYVRRCFVAFEKG 373
>SPBC776.18c |pmh1|mcr1|transcription factor TFIIH complex subunit
Pmh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 318
Score = 26.2 bits (55), Expect = 4.1
Identities = 16/55 (29%), Positives = 23/55 (41%), Gaps = 8/55 (14%)
Frame = +1
Query: 340 CPLCXG-YYIXATTIV----ECLHSFCRSCIIK-HLKAKSYCPV--CEMMINSAK 480
CPLC Y+ + EC H C SC+ + + CP C ++ AK
Sbjct: 13 CPLCQADRYLNPNMKLLINPECYHKMCESCVDRIFTTGPAQCPTPGCNKILRKAK 67
>SPAC3H1.09c |||vacuolar amino acid transporter |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 656
Score = 25.0 bits (52), Expect = 9.5
Identities = 15/53 (28%), Positives = 20/53 (37%)
Frame = -3
Query: 513 VLQCFVKFDIGFCRIYHHFTYRTVALCFKVFNNTTTTKGMQAFHYCGRXYVVP 355
+L V IGF Y F T+ C KV + T + F + VP
Sbjct: 353 ILASIVVSQIGFSSAYISFVASTLQACVKVISTTHREYHLAVFIFIQFLVFVP 405
>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 357
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/37 (32%), Positives = 18/37 (48%), Gaps = 1/37 (2%)
Frame = +2
Query: 287 LPLYRKEHYWVKLTN-I*RAHYVXGTTXMRPQ*WNAC 394
+PL RK HYWV + + Y G ++ + W C
Sbjct: 319 IPLLRKYHYWVLIIEMLINGTYGMGVVLLKFK-WKTC 354
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,573,633
Number of Sequences: 5004
Number of extensions: 51780
Number of successful extensions: 145
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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