Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_P09
(655 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn... 89 4e-19
SPBC887.11 |pus2||tRNA pseudouridylate synthase Pus2 |Schizosacc... 65 7e-12
SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyc... 64 2e-11
SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces pom... 47 3e-06
SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 29 0.77
SPCC306.11 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 25 7.2
SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom... 25 9.5
>SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate
synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 534
Score = 89.4 bits (212), Expect = 4e-19
Identities = 45/127 (35%), Positives = 74/127 (58%), Gaps = 2/127 (1%)
Frame = +1
Query: 280 ERIKRKKMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQR 459
E+ ++K+A L+GY G Y+GMQ NP +TI ++ + ++ + R
Sbjct: 39 EKKPKRKVACLVGYCGSGYHGMQLNPPSKTIEGDLFDAFVKAGAVSSYNADDPKKVALAR 98
Query: 460 SSRTDKGVSAAEQVVSLKLPLQVN--VEXINKRLPXTIKVFAVKRVTNRFNSKSKCNARS 633
++RTDKGV AA V+SLKL ++ +E +N+ LP +I+++ V R N FN ++ C +R
Sbjct: 99 AARTDKGVHAAGNVISLKLIMEDEKLIEKVNEHLPPSIRLWDVIRTINSFNPRTYCESRI 158
Query: 634 YSYTLPT 654
Y Y +PT
Sbjct: 159 YEYMVPT 165
>SPBC887.11 |pus2||tRNA pseudouridylate synthase Pus2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 451
Score = 65.3 bits (152), Expect = 7e-12
Identities = 38/125 (30%), Positives = 67/125 (53%), Gaps = 6/125 (4%)
Frame = +1
Query: 298 KMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRSSRTDK 477
++ +LLGYSG Y+G+Q N ++TI Y+ + + A++ R++RTDK
Sbjct: 40 RIIILLGYSGYGYHGIQINNPLKTIEGDVVAVLKKLGYLKTNNID-AEHLCIARAARTDK 98
Query: 478 GVSAAEQVVSLKL----PLQVNV--EXINKRLPXTIKVFAVKRVTNRFNSKSKCNARSYS 639
GV ++SL L PL +++ +N+ L I+V++V FN + C +R+Y
Sbjct: 99 GVHTLRNLISLNLFVDKPLDISLLKTELNEALCSQIRVWSVFPAPKYFNPRISCESRTYE 158
Query: 640 YTLPT 654
Y +P+
Sbjct: 159 YLIPS 163
>SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 413
Score = 64.1 bits (149), Expect = 2e-11
Identities = 39/125 (31%), Positives = 63/125 (50%), Gaps = 5/125 (4%)
Frame = +1
Query: 292 RKKMAM-LLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRSSR 468
RK+M ++ Y G + G+Q N V+TI + Q + ++ + + ++R
Sbjct: 35 RKRMVYCVVSYRGTGFAGLQYNANVKTIQDTLFQAFARVGAVVQVNADSPKKIRMCSAAR 94
Query: 469 TDKGVSAAEQVVSLKL----PLQVNVEXINKRLPXTIKVFAVKRVTNRFNSKSKCNARSY 636
TDKGV A V+ LK+ PL V +N LP I+V+ + R N F+ + C++R Y
Sbjct: 95 TDKGVHAIVNVLGLKVLDNQPLPHVVNLVNDILPPCIRVWKMARTFNSFSPHTVCDSRVY 154
Query: 637 SYTLP 651
Y LP
Sbjct: 155 EYWLP 159
>SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 450
Score = 46.8 bits (106), Expect = 3e-06
Identities = 43/170 (25%), Positives = 72/170 (42%), Gaps = 9/170 (5%)
Frame = +1
Query: 160 KDEENKSIDIVPNKLLTRYNKRRAHRDWETKPPGEIKKPYERIKRKKMAMLLGYSGVEYY 339
K++ + +I P L + A +D + P + +P++ K +A Y G Y
Sbjct: 47 KNKLDNGNEIEPQHLSQELVLQHALQDASSLPKLKPVRPFQLEKTVFVAFKFAYCGWNYN 106
Query: 340 GM--QRNPG-VQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRSSRTDKGVSAAEQVVSL 510
G+ Q P + T+ IT + + F R RTDKGVSA QV+SL
Sbjct: 107 GLAYQLEPTPLPTVEGKVFEALLRAHLIT-----DPSSCSFSRCGRTDKGVSAMGQVISL 161
Query: 511 KL------PLQVNVEXINKRLPXTIKVFAVKRVTNRFNSKSKCNARSYSY 642
+ P+ + + +N+ LP I++ F+++ C R Y Y
Sbjct: 162 NVRHSERRPI-IYCDVLNRLLPADIRITGYAFPPEGFDARFSCKQRHYKY 210
>SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2812
Score = 28.7 bits (61), Expect = 0.77
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = -3
Query: 353 FRCIP*YSTPEYPSSIAIFFLLILSYGFLISP 258
++C Y E+P S+ +FF I+ + F SP
Sbjct: 1520 WKCYKAYDITEFPDSLKLFFSDIMGWNFFKSP 1551
>SPCC306.11 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 283
Score = 25.4 bits (53), Expect = 7.2
Identities = 22/69 (31%), Positives = 28/69 (40%)
Frame = -3
Query: 305 AIFFLLILSYGFLISPGGLVSQSRCARRLLYLVNSLFGTISIDLFSSSFVTSAASITDSK 126
AIFFL SY I G+ C RR + L T DL S+ TS +I++
Sbjct: 11 AIFFLCSFSYVSSIKLCGIPGAPACKRREISLYKRATDTFP-DLSSAIASTSYYNISEGT 69
Query: 125 RALVMAKTL 99
V L
Sbjct: 70 YCHVSGSNL 78
>SPCC132.01c ||SPCC1322.17c|DUF814 family
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1021
Score = 25.0 bits (52), Expect = 9.5
Identities = 19/54 (35%), Positives = 25/54 (46%)
Frame = -3
Query: 293 LLILSYGFLISPGGLVSQSRCARRLLYLVNSLFGTISIDLFSSSFVTSAASITD 132
+LI+ YG L S+ R ARRL V G +S + VTS +I D
Sbjct: 686 VLIMGYGILWQLDEKSSERRKARRLEMEVVETQGKVSELKMEGTSVTSEDNIQD 739
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,525,395
Number of Sequences: 5004
Number of extensions: 48945
Number of successful extensions: 154
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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