Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_P09
(655 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g20370.1 68414.m02541 tRNA pseudouridine synthase family prot... 94 8e-20
At1g76120.2 68414.m08840 tRNA pseudouridine synthase family prot... 83 2e-16
At1g76120.1 68414.m08839 tRNA pseudouridine synthase family prot... 83 2e-16
At2g30320.1 68415.m03690 tRNA pseudouridine synthase family prot... 71 9e-13
At5g35400.1 68418.m04207 tRNA pseudouridine synthase family prot... 38 0.004
At1g34150.1 68414.m04236 tRNA pseudouridine synthase family prot... 37 0.013
At5g57020.1 68418.m07117 myristoyl-CoA:protein N-myristoyltransf... 30 1.2
At2g25730.1 68415.m03084 expressed protein 30 1.2
At1g34650.1 68414.m04309 homeobox-leucine zipper family protein ... 30 1.5
At3g45650.1 68416.m04931 proton-dependent oligopeptide transport... 29 3.6
At2g27980.1 68415.m03391 expressed protein 28 4.7
At1g65790.1 68414.m07466 S-receptor protein kinase, putative sim... 27 8.2
At1g63600.1 68414.m07189 protein kinase-related low similarity t... 27 8.2
>At1g20370.1 68414.m02541 tRNA pseudouridine synthase family protein
similar to SP|Q9WU56 tRNA pseudouridine synthase A (EC
4.2.1.70) (Uracil hydrolyase) {Mus musculus}; contains
Pfam profile PF01416: tRNA pseudouridine synthase
Length = 549
Score = 93.9 bits (223), Expect = 8e-20
Identities = 49/125 (39%), Positives = 71/125 (56%), Gaps = 2/125 (1%)
Frame = +1
Query: 283 RIKRKKMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRS 462
+ +R+K+A++ + GV Y GMQ+NPG +TI + D +N ++ RS
Sbjct: 54 KYRRRKVAIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARS 113
Query: 463 SRTDKGVSAAEQVVSLKLPLQVN--VEXINKRLPXTIKVFAVKRVTNRFNSKSKCNARSY 636
+RTDKGVSA QVVS + + VE +N +LP I+VF KRV F+SK C+ R Y
Sbjct: 114 ARTDKGVSAVGQVVSGRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRY 173
Query: 637 SYTLP 651
Y +P
Sbjct: 174 VYLIP 178
>At1g76120.2 68414.m08840 tRNA pseudouridine synthase family protein
similar to SP|Q9Y606 tRNA pseudouridine synthase A (EC
4.2.1.70) (Uracil hydrolyase) {Homo sapiens}; contains
Pfam profile PF01416: tRNA pseudouridine synthase
Length = 356
Score = 83.0 bits (196), Expect = 2e-16
Identities = 48/122 (39%), Positives = 65/122 (53%), Gaps = 3/122 (2%)
Frame = +1
Query: 295 KKMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRSSRTD 474
+K+A++ + GV Y GMQ+NPG +TI + + + F RS+RTD
Sbjct: 22 QKVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSARTD 81
Query: 475 KGVSAAEQVVSLKL---PLQVNVEXINKRLPXTIKVFAVKRVTNRFNSKSKCNARSYSYT 645
KGVSA QVVS + PL V +N LP I++F K VT F+SK C+ R Y Y
Sbjct: 82 KGVSAVGQVVSGRFIVDPLGF-VNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYL 140
Query: 646 LP 651
LP
Sbjct: 141 LP 142
>At1g76120.1 68414.m08839 tRNA pseudouridine synthase family protein
similar to SP|Q9Y606 tRNA pseudouridine synthase A (EC
4.2.1.70) (Uracil hydrolyase) {Homo sapiens}; contains
Pfam profile PF01416: tRNA pseudouridine synthase
Length = 463
Score = 83.0 bits (196), Expect = 2e-16
Identities = 48/122 (39%), Positives = 65/122 (53%), Gaps = 3/122 (2%)
Frame = +1
Query: 295 KKMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRSSRTD 474
+K+A++ + GV Y GMQ+NPG +TI + + + F RS+RTD
Sbjct: 22 QKVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSARTD 81
Query: 475 KGVSAAEQVVSLKL---PLQVNVEXINKRLPXTIKVFAVKRVTNRFNSKSKCNARSYSYT 645
KGVSA QVVS + PL V +N LP I++F K VT F+SK C+ R Y Y
Sbjct: 82 KGVSAVGQVVSGRFIVDPLGF-VNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYL 140
Query: 646 LP 651
LP
Sbjct: 141 LP 142
>At2g30320.1 68415.m03690 tRNA pseudouridine synthase family protein
similar to pseudouridine synthase [Schizosaccharomyces
pombe] GI:6469502; contains Pfam profile PF01416: tRNA
pseudouridine synthase
Length = 510
Score = 70.5 bits (165), Expect = 9e-13
Identities = 43/139 (30%), Positives = 69/139 (49%), Gaps = 14/139 (10%)
Frame = +1
Query: 277 YERIKRKKMAMLLGYSGVEYYGMQ---RNPGVQTIXXXXXXXXXXXXYITQEDFENAQNA 447
+E ++KK+ + +GY G +Y G+Q +P ++TI I ++ +
Sbjct: 57 WESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKI 116
Query: 448 QFQRSSRTDKGVSAAEQVVSLKLPLQ-----------VNVEXINKRLPXTIKVFAVKRVT 594
+ RSSRTDKGV + +SLK+ + V + I+K LP I+VF+V
Sbjct: 117 GWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSN 176
Query: 595 NRFNSKSKCNARSYSYTLP 651
RF+ + +C R YSY LP
Sbjct: 177 RRFDPRRECTLRKYSYLLP 195
>At5g35400.1 68418.m04207 tRNA pseudouridine synthase family protein
weak similarity to SP|P07649 tRNA pseudouridine
synthase A (EC 4.2.1.70) (Uracil hydrolyase)
{Escherichia coli}; contains Pfam profile PF01416: tRNA
pseudouridine synthase
Length = 420
Score = 38.3 bits (85), Expect = 0.004
Identities = 31/133 (23%), Positives = 53/133 (39%), Gaps = 10/133 (7%)
Frame = +1
Query: 283 RIKRKKMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQR- 459
R +R ++L Y+G + G Q+ P + T+ + + +
Sbjct: 89 RWERVNFKIVLSYNGASFDGWQKQPDLHTVQGVVEKSLGGFVDERKAQLLKKKCKPLEGR 148
Query: 460 ---SSRTDKGVSAAEQVVSLKL------PLQVNVEXINKRLPXTIKVFAVKRVTNRFNSK 612
+ RTDKGVSA QV S P+ + + INK ++V ++ +V+ F+
Sbjct: 149 VLVAGRTDKGVSALNQVCSFYTWRKDIEPIAIE-DAINKDASGKLRVVSISKVSRSFHPN 207
Query: 613 SKCNARSYSYTLP 651
R Y Y P
Sbjct: 208 FSAKWRRYLYIFP 220
>At1g34150.1 68414.m04236 tRNA pseudouridine synthase family protein
similar to pseudouridine synthase 3 [Mus musculus]
GI:9652099; contains Pfam profile PF01416: tRNA
pseudouridine synthase
Length = 446
Score = 36.7 bits (81), Expect = 0.013
Identities = 46/201 (22%), Positives = 80/201 (39%), Gaps = 22/201 (10%)
Frame = +1
Query: 106 LAITSARLLSVMEAADVTKDEENKSIDIVPNKLLTRYNKRRAHRDWETKPPGEIKKPYER 285
L + +A+LLS + + + E + + L R +RR + + P +
Sbjct: 35 LEVENAKLLSQVSSCQCQQMEVKHDRSLSDSSSLVR--RRRVRKGDKNSIPSHL------ 86
Query: 286 IKRKKMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRSS 465
I ++ +A+ + Y G +YG ++ + D +++ + R
Sbjct: 87 ISKRYVALKIMYFGKRFYGFSAEAQMEPSIESEVFKALERTRLLVGD---KKDSCYSRCG 143
Query: 466 RTDKGVSAAEQVVS------LKLP-----LQVN-----------VEXINKRLPXTIKVFA 579
RTDKGVS+ QV++ LK P QVN V +N+ LP I+V
Sbjct: 144 RTDKGVSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIG 203
Query: 580 VKRVTNRFNSKSKCNARSYSY 642
F+++ C AR Y Y
Sbjct: 204 WSPAPIDFHARFSCYAREYKY 224
>At5g57020.1 68418.m07117 myristoyl-CoA:protein
N-myristoyltransferase 1 (NMT1) identical to
N-myristoyltransferase 1 (NMT1) [Arabidopsis thaliana]
GI:7339834
Length = 434
Score = 30.3 bits (65), Expect = 1.2
Identities = 12/45 (26%), Positives = 26/45 (57%)
Frame = +1
Query: 136 VMEAADVTKDEENKSIDIVPNKLLTRYNKRRAHRDWETKPPGEIK 270
++EA + KD+ S++ + + ++ + H+ WET+P G+ K
Sbjct: 18 IVEANPLVKDDT--SLETIVRRFQDSMSEAKTHKFWETQPVGQFK 60
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 30.3 bits (65), Expect = 1.2
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 458 VAPGLTRVCPLLNKLSHLNYLYKSMLXK 541
+A LTR+C H NYLYK ++ K
Sbjct: 2168 IAGALTRICAFFEINRHFNYLYKFLVLK 2195
>At1g34650.1 68414.m04309 homeobox-leucine zipper family protein /
lipid-binding START domain-containing protein similar to
homeobox 1 (GP:12002853) {Picea abies}; contains Pfam
PF00046: Homeobox domain and Pfam PF01852: START domain
Length = 708
Score = 29.9 bits (64), Expect = 1.5
Identities = 22/76 (28%), Positives = 35/76 (46%), Gaps = 1/76 (1%)
Frame = -3
Query: 317 PSSIAIFFLLILSYGFLISPGGLVSQSRCARRLLY-LVNSLFGTISIDLFSSSFVTSAAS 141
PSS+ + L+IL GF+ + GG+V + Y ++ +I + S F+ S S
Sbjct: 590 PSSVGEYKLMILQDGFIDALGGMVVYAPMNLNTAYSAISGQVDPSTIPILPSGFIISRDS 649
Query: 140 ITDSKRALVMAKTLLT 93
S + TLLT
Sbjct: 650 HPSSSEVDGGSMTLLT 665
>At3g45650.1 68416.m04931 proton-dependent oligopeptide transport
(POT) family protein contains Pfam profile: PF00854 POT
family
Length = 558
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +1
Query: 160 KDEENKSIDIVPNKLLTRYNKRRAHRDWETKPPGEIKKPY 279
KD N+SI + P K +N+ ++ E KP G I+ P+
Sbjct: 264 KDYHNESITM-PTKSFRFFNRAALKQEDEVKPDGTIRNPW 302
>At2g27980.1 68415.m03391 expressed protein
Length = 1037
Score = 28.3 bits (60), Expect = 4.7
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +2
Query: 281 KGLREKKWLCCWGTPELNITVCNEIL 358
K L E+KW C G E+N T+ N I+
Sbjct: 794 KELPEEKWFCSLGCEEINTTLGNLIV 819
>At1g65790.1 68414.m07466 S-receptor protein kinase, putative
similar to similar to PIR|T05180|T05180 S-receptor
kinase ARK3 precursor - [Arabidopsis thaliana]
Length = 843
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = -3
Query: 332 STPEYPSSIAIFFLLILSYGFLISPGGL 249
S P Y S IF +LIL F +SP L
Sbjct: 3 SVPNYHHSFFIFLILILFLAFSVSPNTL 30
>At1g63600.1 68414.m07189 protein kinase-related low similarity to
receptor-like protein kinase 5 [Arabidopsis thaliana]
GI:13506747; contains Pfam profile: PF01657 Domain of
unknown function DUF26
Length = 302
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -3
Query: 524 CKGSLSETTCSAADTPLSVLELRWNCAF 441
C+G +SET+CS + LE+ NC +
Sbjct: 88 CRGDISETSCSDC-VQTATLEISRNCTY 114
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,917,335
Number of Sequences: 28952
Number of extensions: 246423
Number of successful extensions: 764
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 745
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 758
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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