BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P03_F_O21 (653 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_5301| Best HMM Match : No HMM Matches (HMM E-Value=.) 101 5e-22 SB_40157| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.5 SB_19140| Best HMM Match : Cyclotide (HMM E-Value=2.8) 29 4.4 >SB_5301| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 281 Score = 101 bits (242), Expect = 5e-22 Identities = 45/113 (39%), Positives = 71/113 (62%) Frame = +2 Query: 263 IASIQRQGAQGDKTPILXKTTSLEVAACDVGDAVFHSKPKILFCDLTIPLGETKTFWYXE 442 + S+ R + + +TS V+ + G + ++PKILFCDL + GE+KTF Y E Sbjct: 82 VKSMDRITENSNHELVSDSSTSFTVSKGEHGHCILSTQPKILFCDLELAPGESKTFLYTE 141 Query: 443 SLPIEAPPSYRGTSVKYSYKITIATQKVGSHIKMVRIPFXVLPISPIINMQDL 601 ++P E PPSYRG +VKYSYK+T+ T +V S I+++R+P +L + + +Q L Sbjct: 142 TIPSEGPPSYRGQAVKYSYKLTLGTSRVQSQIRLIRLPLRILVVQGLSEIQHL 194 Score = 41.5 bits (93), Expect = 6e-04 Identities = 27/69 (39%), Positives = 33/69 (47%), Gaps = 13/69 (18%) Frame = +1 Query: 100 MIELSAKLTTGTVYLAGEAIECCISFCY-----TAQPEHRNSQSH--------SDTLXNL 240 MIE+ A T G VY AGE++ C I F T + NS H + + L Sbjct: 1 MIEVKASTTRGAVYFAGESVRCEIVFTNVGDQDTDESMELNSLDHAKDINKGRTSHIERL 60 Query: 241 AWASAQFHC 267 AWASAQ HC Sbjct: 61 AWASAQIHC 69 >SB_40157| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 312 Score = 29.5 bits (63), Expect = 2.5 Identities = 22/89 (24%), Positives = 41/89 (46%) Frame = +2 Query: 350 VGDAVFHSKPKILFCDLTIPLGETKTFWYXESLPIEAPPSYRGTSVKYSYKITIATQKVG 529 +G + + S K + D T+ +G++ LP+ + R S K S I++ V Sbjct: 220 LGKSQYSSFLKDVIKDRTVTIGKSLKH---NKLPLFRKQASRNKS-KQSKTISLLQNNVA 275 Query: 530 SHIKMVRIPFXVLPISPIINMQDLAALCG 616 ++ R + I ++N+Q L +LCG Sbjct: 276 LFAQLYRSSMVTVEIKKLLNIQFLVSLCG 304 >SB_19140| Best HMM Match : Cyclotide (HMM E-Value=2.8) Length = 168 Score = 28.7 bits (61), Expect = 4.4 Identities = 8/23 (34%), Positives = 15/23 (65%) Frame = -2 Query: 598 ILHIYYWAYWQHXKRNSDHFNVR 530 ++H +Y H ++N DH+N+R Sbjct: 59 LIHCFYRPLITHSRKNDDHYNIR 81 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,340,954 Number of Sequences: 59808 Number of extensions: 386752 Number of successful extensions: 1610 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 832 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1593 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1669334250 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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