Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_O12
(618 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identi... 44 6e-05
At3g03910.1 68416.m00405 glutamate dehydrogenase, putative simil... 44 6e-05
At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identi... 44 8e-05
At5g37110.1 68418.m04454 hypothetical protein 28 4.3
At1g51720.1 68414.m05828 glutamate dehydrogenase, putative simil... 28 4.3
At4g01380.1 68417.m00178 plastocyanin-like domain-containing pro... 27 7.5
At2g20650.2 68415.m02422 zinc finger (C3HC4-type RING finger) fa... 27 7.5
At2g20650.1 68415.m02421 zinc finger (C3HC4-type RING finger) fa... 27 7.5
At1g30420.1 68414.m03718 ATP-binding cassette transport protein,... 27 7.5
At5g40910.1 68418.m04967 disease resistance protein (TIR-NBS-LRR... 27 10.0
At5g40470.1 68418.m04908 expressed protein 27 10.0
At4g25040.1 68417.m03592 integral membrane family protein contai... 27 10.0
At2g21300.1 68415.m02535 kinesin motor family protein contains P... 27 10.0
At2g05080.1 68415.m00530 hypothetical protein low similarity to ... 27 10.0
At2g04460.1 68415.m00450 expressed protein 27 10.0
At1g11990.1 68414.m01385 expressed protein contains Pfam PF03138... 27 10.0
>At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identical
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 44.4 bits (100), Expect = 6e-05
Identities = 19/52 (36%), Positives = 30/52 (57%)
Frame = +1
Query: 463 LEIQFPLRRDSGDYEMILXYRAQHSTHRTPTKGGIRFSTDVTRDEVKALSAL 618
++++ + +D G + +R QH R P KGGIR+ +V DEV AL+ L
Sbjct: 35 IKVECTIPKDDGTLASFVGFRVQHDNARGPMKGGIRYHPEVDPDEVNALAQL 86
>At3g03910.1 68416.m00405 glutamate dehydrogenase, putative similar
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 44.4 bits (100), Expect = 6e-05
Identities = 19/52 (36%), Positives = 30/52 (57%)
Frame = +1
Query: 463 LEIQFPLRRDSGDYEMILXYRAQHSTHRTPTKGGIRFSTDVTRDEVKALSAL 618
++++ + +D G + +R QH R P KGGIR+ +V DEV AL+ L
Sbjct: 35 IKVECTIPKDDGTLASFVGFRVQHDNARGPMKGGIRYHPEVEPDEVNALAQL 86
>At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identical
to glutamate dehydrogenase 2 (GDH 2) [Arabidopsis
thaliana] SWISS-PROT:Q38946
Length = 411
Score = 44.0 bits (99), Expect = 8e-05
Identities = 19/52 (36%), Positives = 30/52 (57%)
Frame = +1
Query: 463 LEIQFPLRRDSGDYEMILXYRAQHSTHRTPTKGGIRFSTDVTRDEVKALSAL 618
++++ + +D G + +R QH R P KGGIR+ +V DEV AL+ L
Sbjct: 35 IKVECTIPKDDGTLVSYIGFRVQHDNARGPMKGGIRYHPEVDPDEVNALAQL 86
>At5g37110.1 68418.m04454 hypothetical protein
Length = 1307
Score = 28.3 bits (60), Expect = 4.3
Identities = 24/59 (40%), Positives = 30/59 (50%), Gaps = 5/59 (8%)
Frame = +1
Query: 259 SHEIPDKLKDIPTSANPKFFHMV-EYFFHRACQVVEDK--LVEDLKSRTP--IEXKKXK 420
S EIPDKLKD P+ F ++ E H C VV K +E+ K RT +E K K
Sbjct: 532 SAEIPDKLKD------PELFEVIKESMVHGPCGVVNPKCPCMENGKRRTDDFVEKKDFK 584
>At1g51720.1 68414.m05828 glutamate dehydrogenase, putative similar
to NADP-specific glutatamate dehydrogenase (NADP-GDH)
SP:P28724 [Giardia lamblia (Giardia intestinalis)]
Length = 637
Score = 28.3 bits (60), Expect = 4.3
Identities = 16/58 (27%), Positives = 28/58 (48%)
Frame = +1
Query: 436 KLMEPCDHILEIQFPLRRDSGDYEMILXYRAQHSTHRTPTKGGIRFSTDVTRDEVKAL 609
+L+EP + ++ + P D G+ + +R Q + P +GGIRF + K L
Sbjct: 238 RLLEP-ERMIVFRVPWIDDRGETHVNRGFRVQFNQALGPCRGGIRFHPSMNLSIAKFL 294
>At4g01380.1 68417.m00178 plastocyanin-like domain-containing
protein
Length = 210
Score = 27.5 bits (58), Expect = 7.5
Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = +3
Query: 126 HHAASQEYRQVSGSAAQEFCSK*SPQ--YNVPNHTSW 230
H+ + QVSG+ EFC SP+ YN + SW
Sbjct: 38 HNHNLNDVTQVSGALEYEFCDSSSPKAVYNPGHDISW 74
>At2g20650.2 68415.m02422 zinc finger (C3HC4-type RING finger)
family protein
Length = 559
Score = 27.5 bits (58), Expect = 7.5
Identities = 13/27 (48%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = -3
Query: 595 HLWLRP-LRIEYLLWLESCVWNVVRDS 518
H ++RP L + Y W+ V NVVRDS
Sbjct: 392 HNYMRPILLLMYSFWIPQIVANVVRDS 418
>At2g20650.1 68415.m02421 zinc finger (C3HC4-type RING finger)
family protein
Length = 559
Score = 27.5 bits (58), Expect = 7.5
Identities = 13/27 (48%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = -3
Query: 595 HLWLRP-LRIEYLLWLESCVWNVVRDS 518
H ++RP L + Y W+ V NVVRDS
Sbjct: 392 HNYMRPILLLMYSFWIPQIVANVVRDS 418
>At1g30420.1 68414.m03718 ATP-binding cassette transport protein,
putative contains Pfam profiles PF00005: ABC
transporter, PF00664: ABC transporter transmembrane
region
Length = 1488
Score = 27.5 bits (58), Expect = 7.5
Identities = 12/27 (44%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = +1
Query: 184 VQNEAL-NTMFRIIPAGVNVCCRTYAS 261
+ NE+L N + +P GVN+C YAS
Sbjct: 207 LNNESLDNVEYDALPGGVNICPERYAS 233
>At5g40910.1 68418.m04967 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Non-consensus TT donor splice site at exon 1
Length = 1104
Score = 27.1 bits (57), Expect = 10.0
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 3/44 (6%)
Frame = -1
Query: 252 CAAADIHSSWYDSEHCIEGFILNRILERRN---H*LGDILEMQH 130
C I + YDS+ C++ +L++IL +++ H LG I E H
Sbjct: 238 CFMGTIDVNDYDSKLCLQNKLLSKILNQKDMKIHHLGAIEEWLH 281
>At5g40470.1 68418.m04908 expressed protein
Length = 496
Score = 27.1 bits (57), Expect = 10.0
Identities = 8/25 (32%), Positives = 19/25 (76%)
Frame = -1
Query: 324 HVEELRIRTCRNIFELIWNLMTSVC 250
++EE+ +RTCR+I +++ ++ +C
Sbjct: 250 NLEEIELRTCRSIVDVVLLKVSEIC 274
>At4g25040.1 68417.m03592 integral membrane family protein contains
TIGRFAM TIGR01569 : plant integral membrane protein
TIGR01569; contains Pfam PF04535 : Domain of unknown
function (DUF588)
Length = 170
Score = 27.1 bits (57), Expect = 10.0
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = -1
Query: 597 FISGYVR*ESNTSFGWSPVCG 535
F GYV N GW P+CG
Sbjct: 113 FAEGYVGKYGNKQAGWLPICG 133
>At2g21300.1 68415.m02535 kinesin motor family protein contains Pfam
profile: kinesin motor domain PF00225
Length = 862
Score = 27.1 bits (57), Expect = 10.0
Identities = 13/46 (28%), Positives = 22/46 (47%)
Frame = -3
Query: 586 LRPLRIEYLLWLESCVWNVVRDSXISFRNRQNRASEEIESQECDRM 449
LRPL + +L E+ W + D+ + +RN S + DR+
Sbjct: 31 LRPLNEKEILANEAADWECINDTTVLYRNTLREGSTFPSAYSFDRV 76
>At2g05080.1 68415.m00530 hypothetical protein low similarity to
SP|Q9UUA2 DNA repair and recombination protein pif1,
mitochondrial precursor {Schizosaccharomyces pombe}
Length = 1238
Score = 27.1 bits (57), Expect = 10.0
Identities = 17/38 (44%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = +1
Query: 259 SHEIPDKLKDIPTSANPKFFHMV-EYFFHRACQVVEDK 369
S EIPDKLKD P+ F ++ E H C VV K
Sbjct: 399 SAEIPDKLKD------PELFEVIKEMMVHGPCGVVNPK 430
>At2g04460.1 68415.m00450 expressed protein
Length = 148
Score = 27.1 bits (57), Expect = 10.0
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = -1
Query: 261 TSVCAAADIHSSWYDSEHCIEGFILNRILERR 166
T+V A +++ W ++C +G ILNR++ R
Sbjct: 69 TNVYAVGSMNT-WAQGDYCCKGLILNRLVHDR 99
>At1g11990.1 68414.m01385 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'growth regulator protein -related' based on similarity
to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana
tabacum], which, due to scienitific fraud was retracted.
Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908.
PMID:10400497.; expression supported by MPSS
Length = 590
Score = 27.1 bits (57), Expect = 10.0
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +3
Query: 405 RXEKXSSRYSKTYGTMRSHS*DSISSEARFWRLRNDIXLSRTTFHTQD 548
+ EK +Y+ TYG M S + D+++ R R N + T+D
Sbjct: 116 KFEKQKMKYNGTYGRMLSLATDALAEGMRDNRYCNGFDFEQNKLETKD 163
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,985,403
Number of Sequences: 28952
Number of extensions: 257121
Number of successful extensions: 568
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 555
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 568
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1246162608
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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