Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_N24
(653 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g06140.1 68416.m00705 zinc finger (C3HC4-type RING finger) fa... 31 0.51
At4g35240.1 68417.m05009 expressed protein contains Pfam domains... 30 1.2
At5g03200.1 68418.m00268 zinc finger (C3HC4-type RING finger) fa... 30 1.5
At2g11010.1 68415.m01178 hypothetical protein 29 2.7
At5g38990.1 68418.m04717 protein kinase family protein contains ... 28 4.7
At5g03150.1 68418.m00263 zinc finger (C2H2 type) family protein ... 28 4.7
At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam... 28 4.7
At1g27100.1 68414.m03303 expressed protein contains Pfam profil... 27 8.2
>At3g06140.1 68416.m00705 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 Zinc
finger, C3HC4 type (RING finger)
Length = 359
Score = 31.5 bits (68), Expect = 0.51
Identities = 34/141 (24%), Positives = 53/141 (37%), Gaps = 1/141 (0%)
Frame = +2
Query: 233 PQPEAYLFGENADLNFLGSRPTPFPYPPPQ-SNEPTKTLKSLXNIRKESLRFIRCPEPLS 409
PQP Y A N+ G P Y PPQ +P K N +K +
Sbjct: 76 PQPPQYF--TTAQPNWWGPMMRPAYYCPPQPQTQPPKPYLEQQNAKKVR-NDVNVHRDTV 132
Query: 410 KLXDNQIGDGVMKSPESNGKGTYYNIEFTFDCDARCAITVHYFCTEEVTPAGVVYYPRDP 589
+L + + G ++ + F FD + T+ +F EE + +P
Sbjct: 133 RLEVDDLVPG------------HHLVSFVFDALFDGSFTITFFAKEEPNCTIIPQFPE-- 178
Query: 590 TMSSQTYHYKKGANQQFCQIS 652
S +H++KG Q+F Q S
Sbjct: 179 VYSPTRFHFQKGPGQKFLQPS 199
>At4g35240.1 68417.m05009 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 828
Score = 30.3 bits (65), Expect = 1.2
Identities = 20/60 (33%), Positives = 30/60 (50%)
Frame = +2
Query: 149 SNHAYKYPPRSGNYFGSHFIMGGERFDTPQPEAYLFGENADLNFLGSRPTPFPYPPPQSN 328
S+ +Y YPP++ +YFG + G P P Y +A ++P P P PP+SN
Sbjct: 224 SSSSYPYPPQN-SYFGYSNPVPG-----PGP-GYYGSSSASTTAAATKPPPPPPSPPRSN 276
>At5g03200.1 68418.m00268 zinc finger (C3HC4-type RING finger)
family protein contains weak similarity to zinc finger
proteins
Length = 337
Score = 29.9 bits (64), Expect = 1.5
Identities = 17/56 (30%), Positives = 28/56 (50%)
Frame = +2
Query: 485 IEFTFDCDARCAITVHYFCTEEVTPAGVVYYPRDPTMSSQTYHYKKGANQQFCQIS 652
+ FTFD ITV +F TE+ + ++ T+ T+ + +G Q+F Q S
Sbjct: 137 VSFTFDASMPGRITVVFFATEDAECN--LRATKEDTLPPITFDFGEGLGQKFIQSS 190
>At2g11010.1 68415.m01178 hypothetical protein
Length = 693
Score = 29.1 bits (62), Expect = 2.7
Identities = 30/109 (27%), Positives = 48/109 (44%), Gaps = 2/109 (1%)
Frame = +2
Query: 179 SGNYFGSH--FIMGGERFDTPQPEAYLFGENADLNFLGSRPTPFPYPPPQSNEPTKTLKS 352
S N+F H F+ DT +PE+ F E N + +RP P S T TL+
Sbjct: 46 STNFFFEHRIFLFSTMNPDTERPESPSFNECGYSNDICTRPNDIAGPHYMS---TCTLQC 102
Query: 353 LXNIRKESLRFIRCPEPLSKLXDNQIGDGVMKSPESNGKGTYYNIEFTF 499
L +++ C P + ++++ G M+SPE + G + E F
Sbjct: 103 LERLKE------LCRIPPEIMTESKM-HGPMESPEDHRSGYFCVYEIYF 144
>At5g38990.1 68418.m04717 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 880
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/26 (50%), Positives = 14/26 (53%)
Frame = +2
Query: 263 NADLNFLGSRPTPFPYPPPQSNEPTK 340
N+D N G P P PPPQS P K
Sbjct: 405 NSDGNLAGLNPIPQLSPPPQSITPLK 430
>At5g03150.1 68418.m00263 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 503
Score = 28.3 bits (60), Expect = 4.7
Identities = 16/52 (30%), Positives = 24/52 (46%), Gaps = 2/52 (3%)
Frame = +2
Query: 311 PPPQSNEPTKTLKSLXNIRKESLR--FIRCPEPLSKLXDNQIGDGVMKSPES 460
PPP+S+ P + L N LR R P PLS + + + + +S S
Sbjct: 409 PPPRSSSPMMIQQQLNNFNTNVLRENHNRAPPPLSGVSTSSVDNNPFQSNRS 460
>At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to adenosine monophosphate binding
protein 1 AMPBP1 (AMPBP1) GI:20799710
Length = 556
Score = 28.3 bits (60), Expect = 4.7
Identities = 23/72 (31%), Positives = 34/72 (47%), Gaps = 3/72 (4%)
Frame = +2
Query: 395 PEPLSKLXDNQIGDGVMKSPE---SNGKGTYYNIEFTFDCDARCAITVHYFCTEEVTPAG 565
PEPL++ + M E + GK + I T +CDA I+V+Y +P G
Sbjct: 148 PEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDA---ISVNYTSGTTSSPKG 204
Query: 566 VVYYPRDPTMSS 601
VVY R ++S
Sbjct: 205 VVYSHRGAYLNS 216
>At1g27100.1 68414.m03303 expressed protein contains Pfam profile:
PF04601 protein of unknown function (DUF569
Length = 519
Score = 27.5 bits (58), Expect = 8.2
Identities = 17/60 (28%), Positives = 27/60 (45%), Gaps = 1/60 (1%)
Frame = +2
Query: 311 PPPQSNEPTKTLKSLXN-IRKESLRFIRCPEPLSKLXDNQIGDGVMKSPESNGKGTYYNI 487
PPP++ P + + + ES P S L D D V P+S+G+ YY++
Sbjct: 389 PPPKTTSPPPHRRPTSSPLSAES------PRTSSSLSDRSDSDSVESPPKSDGRTIYYHV 442
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,667,328
Number of Sequences: 28952
Number of extensions: 266156
Number of successful extensions: 919
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 774
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 910
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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