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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P03_F_L16
         (655 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_2299| Best HMM Match : No HMM Matches (HMM E-Value=.)               64   1e-10
SB_11299| Best HMM Match : No HMM Matches (HMM E-Value=.)              34   0.12 
SB_36169| Best HMM Match : DUF676 (HMM E-Value=0)                      33   0.20 
SB_35177| Best HMM Match : No HMM Matches (HMM E-Value=.)              32   0.47 
SB_33678| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   0.82 
SB_56543| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.4  
SB_18764| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.5  
SB_57728| Best HMM Match : Usp (HMM E-Value=3.9e-20)                   29   3.3  
SB_53556| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.4  
SB_23642| Best HMM Match : Neuromodulin (HMM E-Value=4.7)              29   4.4  
SB_3218| Best HMM Match : Ribosomal_S25 (HMM E-Value=0.65)             29   4.4  
SB_33496| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   5.8  
SB_31640| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   5.8  

>SB_2299| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 271

 Score = 63.7 bits (148), Expect = 1e-10
 Identities = 33/62 (53%), Positives = 38/62 (61%), Gaps = 2/62 (3%)
 Frame = +3

Query: 258 DWQCN--KCANVNWARRQTCNVCNAPKFGEVEARTGYGGGYNERGVVXYRRREHSSDDEY 431
           DW C   KC N+N+ARR +CN C            GYGGGYNERG V Y+ +E  SDDEY
Sbjct: 12  DWVCPDPKCGNMNFARRSSCNRCGR----------GYGGGYNERGTVEYKPKE-ESDDEY 60

Query: 432 DE 437
           DE
Sbjct: 61  DE 62



 Score = 59.3 bits (137), Expect = 3e-09
 Identities = 20/30 (66%), Positives = 26/30 (86%)
 Frame = +3

Query: 72  KMNDGDWICSDPSCGNINFARRASCYRCNK 161
           K++DGDW+C DP CGN+NFARR+SC RC +
Sbjct: 7   KLSDGDWVCPDPKCGNMNFARRSSCNRCGR 36


>SB_11299| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 3762

 Score = 33.9 bits (74), Expect = 0.12
 Identities = 29/144 (20%), Positives = 57/144 (39%), Gaps = 6/144 (4%)
 Frame = +3

Query: 90   WICSDPSCGNINFARRASCYRCNKDRPDGVKPSKKKLGTEIGKSAAEKSRGLFNADDWQC 269
            W C+  SCG +N A+   C+ C+ D+    KP+ K +  ++             ++ WQC
Sbjct: 3008 WDCA--SCGCVNSAKSHQCFGCSLDKVGLEKPAPKSMIQDL-------------SEKWQC 3052

Query: 270  NKCANVNWARRQTCNVC------NAPKFGEVEARTGYGGGYNERGVVXYRRREHSSDDEY 431
            + C   +   R  C  C       +P   E   R     G +E+       +   S+  Y
Sbjct: 3053 DSCYVHHPDERGFCLACGNSRYKGSPPSDEQSKRLQQPEGTDEQDAPNSPEKAVQSEPWY 3112

Query: 432  DEFGXKKRKRKSEQQDKNKTQAHQ 503
              +   +  ++ E+++  +   H+
Sbjct: 3113 WTWLFSRPPQQPEEKESEEANEHE 3136



 Score = 31.5 bits (68), Expect = 0.62
 Identities = 14/43 (32%), Positives = 20/43 (46%)
 Frame = +3

Query: 258  DWQCNKCANVNWARRQTCNVCNAPKFGEVEARTGYGGGYNERG 386
            +W C  C   N A  +TC  C  PK G  +  T +  G  ++G
Sbjct: 2118 EWDCETCLVRNTAESKTCPACQTPKPGATQ--TSHLTGVTKQG 2158


>SB_36169| Best HMM Match : DUF676 (HMM E-Value=0)
          Length = 2442

 Score = 33.1 bits (72), Expect = 0.20
 Identities = 25/88 (28%), Positives = 33/88 (37%), Gaps = 5/88 (5%)
 Frame = +3

Query: 84   GDWICSDPSCGNINFARRASCYRCNKDRPDGVKPSKKKLGTEIGKSAAEKSRGLFNAD-- 257
            G W C   +C   N A   +C  C   +P G KP+          + +  S GLFN    
Sbjct: 1726 GSWECG--TCMIQNKADVTTCVACEASKP-GSKPAPS--------ATSSTSNGLFNKQAP 1774

Query: 258  ---DWQCNKCANVNWARRQTCNVCNAPK 332
                W C+ C   N      C  C +PK
Sbjct: 1775 PTGSWTCDTCWISNKEADSRCAACQSPK 1802



 Score = 33.1 bits (72), Expect = 0.20
 Identities = 25/94 (26%), Positives = 31/94 (32%), Gaps = 2/94 (2%)
 Frame = +3

Query: 81   DGDWICSDPSCGNINFARRASCYRCNKDRPDGVKPSKKKLGTEIGKSAAEKSRGLFNAD- 257
            +G W C   +C   N      C  C   +P G   +  K       S+       F    
Sbjct: 1830 EGSWECD--TCMVNNKGSDTKCVACETAKP-GANSTASKTPAFGASSSTFPVLEKFAPPP 1886

Query: 258  -DWQCNKCANVNWARRQTCNVCNAPKFGEVEART 356
              W C+ C  VN A    C  C  PK G   A T
Sbjct: 1887 GSWTCDTCMIVNKAEHLKCLACETPKPGAKPAET 1920



 Score = 32.3 bits (70), Expect = 0.35
 Identities = 22/66 (33%), Positives = 29/66 (43%), Gaps = 4/66 (6%)
 Frame = +3

Query: 165  RPDGVKPSKKKLGTEIGKSAAEKSRGL--FNAD--DWQCNKCANVNWARRQTCNVCNAPK 332
            +P  VKPS+    ++  KS+A  +  L  F      W+C  C   N A   TC  C A K
Sbjct: 1692 KPASVKPSENSTRSDDKKSSAPATNLLEAFRPPPGSWECGTCMIQNKADVTTCVACEASK 1751

Query: 333  FGEVEA 350
             G   A
Sbjct: 1752 PGSKPA 1757


>SB_35177| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1293

 Score = 31.9 bits (69), Expect = 0.47
 Identities = 12/43 (27%), Positives = 25/43 (58%)
 Frame = +3

Query: 366  GGYNERGVVXYRRREHSSDDEYDEFGXKKRKRKSEQQDKNKTQ 494
            G    +  +   RR+ SS  E D +  K+R+  +EQ++++K++
Sbjct: 883  GSVGSKADIEDNRRKRSSSRESDRYSIKRRRHDNEQEERSKSK 925


>SB_33678| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1308

 Score = 31.1 bits (67), Expect = 0.82
 Identities = 18/70 (25%), Positives = 32/70 (45%)
 Frame = +3

Query: 282 NVNWARRQTCNVCNAPKFGEVEARTGYGGGYNERGVVXYRRREHSSDDEYDEFGXKKRKR 461
           +V W  R    +C +   G V   +  GGG  E+ V   +++ +++ D  D FG +  + 
Sbjct: 313 DVKWCPRNPAMICTSSFDGHVSCFSLMGGGATEQAV--EQQKVNNAFDVDDPFGSQLNQS 370

Query: 462 KSEQQDKNKT 491
             +QQ    T
Sbjct: 371 PPQQQPAETT 380


>SB_56543| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1076

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 11/28 (39%), Positives = 15/28 (53%)
 Frame = +3

Query: 90  WICSDPSCGNINFARRASCYRCNKDRPD 173
           W+C  P C  IN   R  C  C ++RP+
Sbjct: 259 WVC--PQCTLINIPTRPGCAMCGEERPE 284


>SB_18764| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 284

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 9/22 (40%), Positives = 13/22 (59%)
 Frame = +3

Query: 255 DDWQCNKCANVNWARRQTCNVC 320
           D W+C+KC  +N    + C VC
Sbjct: 156 DSWECDKCKTMNHPMTRRCVVC 177


>SB_57728| Best HMM Match : Usp (HMM E-Value=3.9e-20)
          Length = 744

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = +3

Query: 360 YGGGYNERGVVXYRRREHSSDDEYD 434
           Y GG N+  V+  +   H  DD+YD
Sbjct: 196 YAGGDNDDDVILMKAESHDDDDDYD 220


>SB_53556| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1524

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 14/43 (32%), Positives = 19/43 (44%), Gaps = 2/43 (4%)
 Frame = +3

Query: 357  GYGGGYNERGVVXYRRREHSSD--DEYDEFGXKKRKRKSEQQD 479
            GY  GYN+      R  E+  D  D  D +G   R  +S  +D
Sbjct: 1382 GYSNGYNDNQPKEKRSSEYQDDPRDNRDSYGDYSRSSRSSYED 1424


>SB_23642| Best HMM Match : Neuromodulin (HMM E-Value=4.7)
          Length = 286

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 14/42 (33%), Positives = 22/42 (52%)
 Frame = +3

Query: 366 GGYNERGVVXYRRREHSSDDEYDEFGXKKRKRKSEQQDKNKT 491
           GG +  G V   +R+++S  E  +   KK  RKS   DK ++
Sbjct: 4   GGISIEGNVSSNQRDNNSQPEEAQIHSKKEPRKSSGSDKMRS 45


>SB_3218| Best HMM Match : Ribosomal_S25 (HMM E-Value=0.65)
          Length = 290

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 10/28 (35%), Positives = 20/28 (71%)
 Frame = +3

Query: 405 REHSSDDEYDEFGXKKRKRKSEQQDKNK 488
           R+    ++ +EF   K+K+K++Q++KNK
Sbjct: 172 RDQIESEQSEEFVKSKKKKKNKQKEKNK 199


>SB_33496| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1082

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +3

Query: 399 RRREHSSDDEYDEFGXKKRKRKSEQQDKNKT 491
           RRRE    D+  +   K++KR+ EQ+DK  +
Sbjct: 459 RRRESGDTDDSVKARKKEKKRREEQRDKESS 489


>SB_31640| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 3003

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 10/31 (32%), Positives = 18/31 (58%), Gaps = 2/31 (6%)
 Frame = +3

Query: 261  WQCNKCANVNWARRQ--TCNVCNAPKFGEVE 347
            +QC+KC ++N+  R    CN C   K+ + +
Sbjct: 1881 YQCHKCRSINYDERDPFLCNACGFCKYAKFD 1911


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,053,012
Number of Sequences: 59808
Number of extensions: 333165
Number of successful extensions: 1216
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1081
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1209
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1669334250
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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