Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_L10
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g40290.2 68415.m04961 eukaryotic translation initiation facto... 153 7e-38
At2g40290.1 68415.m04960 eukaryotic translation initiation facto... 153 7e-38
At5g05470.1 68418.m00589 eukaryotic translation initiation facto... 151 5e-37
At3g23700.1 68416.m02980 S1 RNA-binding domain-containing protei... 31 0.51
At3g59960.1 68416.m06692 SET domain-containing protein low simil... 31 0.67
At5g59090.1 68418.m07403 subtilase family protein contains simil... 29 2.7
At3g59670.1 68416.m06657 expressed protein ; expression supporte... 29 3.6
At3g42820.1 68416.m04484 hypothetical protein hypothetical prote... 29 3.6
At1g74830.1 68414.m08670 expressed protein contains Pfam profile... 28 4.7
At3g06880.1 68416.m00817 transducin family protein / WD-40 repea... 27 8.2
>At2g40290.2 68415.m04961 eukaryotic translation initiation factor 2
subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
putative similar to Swiss-Prot:P05198 eukaryotic
translation initiation factor 2 subunit 1 (Eukaryotic
translation initiation factor 2 alpha subunit)
(eIF-2-alpha) (EIF- 2alpha) (EIF-2A) [Homo sapiens]
Length = 241
Score = 153 bits (372), Expect = 7e-38
Identities = 87/164 (53%), Positives = 106/164 (64%), Gaps = 1/164 (0%)
Frame = +2
Query: 161 KYPEVEDVVMVNVRSIAEMGAYVHLLEYNNIEGMXXXXXXXXXXXXXXNKLIRVGKTEPV 340
KYPEV+ VM+ V++IA+MGAYV LLEYNNIEGM + LI+VG+ EPV
Sbjct: 16 KYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVSSLIKVGRIEPV 75
Query: 341 VVIRVDKEKGYIDLSKRRVSAEDIYKCTERYAKAKAVNSILRHVAELLHYETSEQLEELY 520
+V+RVDKEKGYIDLSKRRVS EDI C ERY K+K V+SI+RHVAE L S LE+LY
Sbjct: 76 MVLRVDKEKGYIDLSKRRVSEEDIQTCEERYNKSKLVHSIMRHVAETL----SIDLEDLY 131
Query: 521 KKTAWYFEXKYKKKASAYDFFKQAAVDP-SVLDECGLHEETKDV 649
W Y++ A++ FK DP SVL L E K+V
Sbjct: 132 VNIGW---PLYRRHGHAFEAFKILVTDPDSVLGP--LTREIKEV 170
>At2g40290.1 68415.m04960 eukaryotic translation initiation factor 2
subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
putative similar to Swiss-Prot:P05198 eukaryotic
translation initiation factor 2 subunit 1 (Eukaryotic
translation initiation factor 2 alpha subunit)
(eIF-2-alpha) (EIF- 2alpha) (EIF-2A) [Homo sapiens]
Length = 344
Score = 153 bits (372), Expect = 7e-38
Identities = 87/164 (53%), Positives = 106/164 (64%), Gaps = 1/164 (0%)
Frame = +2
Query: 161 KYPEVEDVVMVNVRSIAEMGAYVHLLEYNNIEGMXXXXXXXXXXXXXXNKLIRVGKTEPV 340
KYPEV+ VM+ V++IA+MGAYV LLEYNNIEGM + LI+VG+ EPV
Sbjct: 16 KYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVSSLIKVGRIEPV 75
Query: 341 VVIRVDKEKGYIDLSKRRVSAEDIYKCTERYAKAKAVNSILRHVAELLHYETSEQLEELY 520
+V+RVDKEKGYIDLSKRRVS EDI C ERY K+K V+SI+RHVAE L S LE+LY
Sbjct: 76 MVLRVDKEKGYIDLSKRRVSEEDIQTCEERYNKSKLVHSIMRHVAETL----SIDLEDLY 131
Query: 521 KKTAWYFEXKYKKKASAYDFFKQAAVDP-SVLDECGLHEETKDV 649
W Y++ A++ FK DP SVL L E K+V
Sbjct: 132 VNIGW---PLYRRHGHAFEAFKILVTDPDSVLGP--LTREIKEV 170
>At5g05470.1 68418.m00589 eukaryotic translation initiation factor 2
subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
putative similar to SWISS-PROT:P20459 eukaryotic
translation initiation factor 2 alpha subunit (eIF-2-
alpha) [Saccharomyces cerevisiae]; identical to cDNA
cohesin GI:6682280
Length = 344
Score = 151 bits (365), Expect = 5e-37
Identities = 86/178 (48%), Positives = 109/178 (61%), Gaps = 1/178 (0%)
Frame = +2
Query: 116 NKHTRCRCRVDFINEKYPEVEDVVMVNVRSIAEMGAYVHLLEYNNIEGMXXXXXXXXXXX 295
N CR+ +YP+V+ VM+ V++IA+MGAYV LLEYNNIEGM
Sbjct: 3 NPAPNLECRM--YESRYPDVDMAVMIQVKTIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
Query: 296 XXXNKLIRVGKTEPVVVIRVDKEKGYIDLSKRRVSAEDIYKCTERYAKAKAVNSILRHVA 475
+ LI+VG+TEPV+V+RVD+E+GYIDLSKRRVS ED C ERY K+K V+SI+RHVA
Sbjct: 61 RSISSLIKVGRTEPVMVLRVDRERGYIDLSKRRVSDEDKEACEERYNKSKLVHSIMRHVA 120
Query: 476 ELLHYETSEQLEELYKKTAWYFEXKYKKKASAYDFFKQAAVDP-SVLDECGLHEETKD 646
E + + LEELY W YKK A++ FK DP SV D L E K+
Sbjct: 121 ETVGVD----LEELYVNIGW---PLYKKHGHAFEAFKIVVTDPDSVFD--ALTREVKE 169
>At3g23700.1 68416.m02980 S1 RNA-binding domain-containing protein
contains Pfam domain, PF00575: S1 RNA binding domain
Length = 392
Score = 31.5 bits (68), Expect = 0.51
Identities = 21/89 (23%), Positives = 40/89 (44%), Gaps = 3/89 (3%)
Frame = +2
Query: 173 VEDVVMVNVRSIAEMGAYVHLL---EYNNIEGMXXXXXXXXXXXXXXNKLIRVGKTEPVV 343
V DV V S+ + GA++HL ++ G+ ++R G V+
Sbjct: 195 VGDVFNGRVGSVEDYGAFIHLRFDDGLYHLTGLVHVSEVSWDYVQDVRDVLRDGDEVRVI 254
Query: 344 VIRVDKEKGYIDLSKRRVSAEDIYKCTER 430
V +DKEK I LS +++ + + + ++
Sbjct: 255 VTNIDKEKSRITLSIKQLEDDPLLETLDK 283
>At3g59960.1 68416.m06692 SET domain-containing protein low
similarity to huntingtin interacting protein 1 [Homo
sapiens] GI:12697196; contains Pfam profile PF00856: SET
domain
Length = 352
Score = 31.1 bits (67), Expect = 0.67
Identities = 18/73 (24%), Positives = 34/73 (46%), Gaps = 3/73 (4%)
Frame = +2
Query: 416 KCTERYAKAKAVNSILRHVAELLHYETSEQLEE---LYKKTAWYFEXKYKKKASAYDFFK 586
K ++R K++ + + + EL E+ ++L ++ K Y + K KKK + F
Sbjct: 6 KGSDRNQIRKSLRKLKKQIGELEKLESPDRLNNVKPIFIKRNIYLKKKLKKKVKDHGIFC 65
Query: 587 QAAVDPSVLDECG 625
++DP CG
Sbjct: 66 SCSLDPGSSTLCG 78
>At5g59090.1 68418.m07403 subtilase family protein contains
similarity to prepro-cucumisin GI:807698 from [Cucumis
melo]
Length = 736
Score = 29.1 bits (62), Expect = 2.7
Identities = 24/74 (32%), Positives = 34/74 (45%)
Frame = -1
Query: 279 SSDSKIMPSILLYSRRWTYAPISAMDLTFTITTSSTSGYFSLIKSTRQRHLVCLFNSFLS 100
S +KI+P L Y A +S D TF++T + T ST + +V S LS
Sbjct: 618 SKKNKILPRNLNYPSM--SAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLS 675
Query: 99 VKY*IKKEYDETVN 58
+K Y +TVN
Sbjct: 676 IKVTPSVLYFKTVN 689
>At3g59670.1 68416.m06657 expressed protein ; expression supported
by MPSS
Length = 510
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
Frame = +2
Query: 548 KYKKKASAYDFFK-QAAVDPSVLDECG 625
+Y K+ YD K +A +DPSVL+ CG
Sbjct: 159 EYPKELELYDQEKLEANIDPSVLESCG 185
>At3g42820.1 68416.m04484 hypothetical protein hypothetical proteins
- Arabidopsis thaliana
Length = 906
Score = 28.7 bits (61), Expect = 3.6
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = -1
Query: 390 RFDKSMYPFSLSTLITTTGSVFPTRINLLMDLIRLLESSDSKIMPSILLYSR 235
RF KSM + I+ G V + ++L DLI ++ SK+M +++ +SR
Sbjct: 744 RFQKSMKKLKSISFISICGGVALSNKDIL-DLIERKKNLTSKVMDALIKFSR 794
>At1g74830.1 68414.m08670 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 542
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/55 (27%), Positives = 31/55 (56%)
Frame = +2
Query: 344 VIRVDKEKGYIDLSKRRVSAEDIYKCTERYAKAKAVNSILRHVAELLHYETSEQL 508
V+ D+EKG SK + +++ + TER + ++ +L+H+A++L E +
Sbjct: 467 VVSADEEKG--SESKEGI-VKELSEITERLSTLQSNGDLLKHIADVLDVSEGEAI 518
>At3g06880.1 68416.m00817 transducin family protein / WD-40 repeat
family protein similar to PAK/PLC-interacting protein 1
(GI:4211689) {Homo sapiens}
Length = 1115
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
Frame = +2
Query: 110 ELNKHTRCRCRVDFINEKYPEV-EDV---VMVNVRSIAEMGAY 226
E K TR RC +F+NE P+ ED+ + N+ +++ A+
Sbjct: 254 EPGKSTRVRCLNEFLNESQPDTREDIGTDTLANIFCVSQQQAH 296
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,129,890
Number of Sequences: 28952
Number of extensions: 249580
Number of successful extensions: 666
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 665
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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