Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_K21
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3F10.04 |gsa1|gsh2|glutathione synthetase large subunit Gsa1... 80 3e-16
SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual 27 1.8
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 27 3.1
SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7 |Schizos... 26 4.1
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 26 4.1
SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyce... 26 4.1
SPAC9.05 |mfh1||ATP-dependent DNA helicase Mfh1 |Schizosaccharom... 26 4.1
SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 25 9.5
SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2 |Schizosacch... 25 9.5
SPBC19C2.13c |||conserved eukaryotic protein|Schizosaccharomyces... 25 9.5
>SPAC3F10.04 |gsa1|gsh2|glutathione synthetase large subunit
Gsa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 79.8 bits (188), Expect = 3e-16
Identities = 39/77 (50%), Positives = 47/77 (61%)
Frame = +2
Query: 419 HPVAVVYYRSGYEPAQYPTAREWDARLRVERSTAIKCPSIHYQLAGTKKVQQALAGPGVL 598
+ VAVVYYR GY YP+ WD RL +E + AIKCPSI LAG+KK+QQ LA L
Sbjct: 283 YEVAVVYYRVGYALDDYPSQEAWDMRLTIENTLAIKCPSISTHLAGSKKIQQVLAESNAL 342
Query: 599 EKFMGSGTSTSRVRDIF 649
E+F+ G VR F
Sbjct: 343 ERFL-EGDELQAVRSTF 358
>SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual
Length = 426
Score = 27.5 bits (58), Expect = 1.8
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = -3
Query: 400 MSLVYVRSLFEYLNFSNSNEYLKKKDMPAEFLAS 299
MS+ Y SLF +F ++KK+ PA FL S
Sbjct: 181 MSVGYTESLFAAFSFLGLLLFIKKQQYPAAFLWS 214
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/38 (36%), Positives = 19/38 (50%), Gaps = 1/38 (2%)
Frame = +2
Query: 416 GHPVAVVYYRSGYEPAQYPTAREWDARLR-VERSTAIK 526
G P + + G EP QY AR+ D+ + V T IK
Sbjct: 95 GFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSEKTGIK 132
>SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 721
Score = 26.2 bits (55), Expect = 4.1
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +1
Query: 247 VKKKYRQFTKTSLRPEKNWQETQRACL 327
V+KK +F + RP+ W E+ AC+
Sbjct: 603 VQKKACEFLLSKQRPDGGWSESYMACV 629
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 26.2 bits (55), Expect = 4.1
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1
Query: 630 EVLVPEPMNFSSTPGPARACWTFF 559
E++ P+ FS+TP R WT F
Sbjct: 3432 EIMKLVPVRFSNTPTTLRLMWTLF 3455
>SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 26.2 bits (55), Expect = 4.1
Identities = 13/45 (28%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +1
Query: 271 TKTSLRPEKNWQETQRACLFFLNIHSNL---KNSNIQIRNERIQV 396
T ++ W E QR CL + H +L K+S+I + E +++
Sbjct: 423 TNNKIQANLGWNERQRQCLSSCSSHLSLALQKSSDIVVAAEEVKL 467
>SPAC9.05 |mfh1||ATP-dependent DNA helicase Mfh1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 834
Score = 26.2 bits (55), Expect = 4.1
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +1
Query: 280 SLRPEKNWQETQRACLFFLNIHSNLKNSNIQI 375
S+ EK ++ +R CL L+IH N + S + +
Sbjct: 662 SIEQEKKLEKYKRVCLRGLDIHRNRRLSQLSV 693
>SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1647
Score = 25.0 bits (52), Expect = 9.5
Identities = 14/44 (31%), Positives = 22/44 (50%)
Frame = -1
Query: 219 VEFFFLI*FDFTYKALLTECRHLLSIFVLFYRKLLESGRFGCDQ 88
++ F L+ T K + + + LL L RKL +S + G DQ
Sbjct: 1579 LQIFILVLMRVTLKEISNKDKLLLQSIFLLSRKLQDSVQTGADQ 1622
>SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 683
Score = 25.0 bits (52), Expect = 9.5
Identities = 14/59 (23%), Positives = 32/59 (54%)
Frame = -3
Query: 478 CGGVLSRLVP*SVVNDCYGMTVPVNDMSLVYVRSLFEYLNFSNSNEYLKKKDMPAEFLA 302
C G ++ VP SV+NDC + + + ++++ Y+ + + +Y +K D+ +L+
Sbjct: 378 CLGDVALKVP-SVINDCISIFLEIYELNISYMVQEVTVVMETVLRKYPQKIDLLLPYLS 435
>SPBC19C2.13c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 366
Score = 25.0 bits (52), Expect = 9.5
Identities = 7/21 (33%), Positives = 15/21 (71%)
Frame = +1
Query: 160 AFCKQCFIREIKSNQKKKFDI 222
A C CF+R I++ +++F++
Sbjct: 29 AVCDSCFVRRIENKIRRQFEL 49
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,620,101
Number of Sequences: 5004
Number of extensions: 54527
Number of successful extensions: 151
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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