Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_K21
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g27380.1 68418.m03269 glutathione synthetase (GSH2) non-conse... 97 9e-21
At1g33770.1 68414.m04174 protein kinase family protein contains ... 31 0.67
At1g68920.2 68414.m07888 basic helix-loop-helix (bHLH) family pr... 30 1.2
At1g68920.1 68414.m07887 basic helix-loop-helix (bHLH) family pr... 30 1.2
At5g67440.1 68418.m08503 phototropic-responsive NPH3 family prot... 29 2.0
At4g31820.1 68417.m04522 phototropic-responsive NPH3 family prot... 29 2.7
At2g23050.1 68415.m02748 phototropic-responsive NPH3 family prot... 29 3.6
At2g40560.1 68415.m05004 protein kinase family protein contains ... 28 6.2
At1g67900.2 68414.m07754 phototropic-responsive NPH3 family prot... 28 6.2
At1g67900.1 68414.m07753 phototropic-responsive NPH3 family prot... 28 6.2
>At5g27380.1 68418.m03269 glutathione synthetase (GSH2)
non-consensus AT donor splice site at exon 6, AC
acceptor splice site at exon 7; identical to
Swiss-Prot:P46416 glutathione synthetase, chloroplast
precursor (Glutathione synthase) (GSH synthetase)
(GSH-S) [Arabidopsis thaliana]
Length = 478
Score = 97.1 bits (231), Expect = 9e-21
Identities = 42/78 (53%), Positives = 57/78 (73%)
Frame = +2
Query: 416 GHPVAVVYYRSGYEPAQYPTAREWDARLRVERSTAIKCPSIHYQLAGTKKVQQALAGPGV 595
G VAVVY+RSGY P +P+ EW+ARL +E S+A+KCPSI Y L G+KK+QQ LA PGV
Sbjct: 265 GQAVAVVYFRSGYTPNDHPSESEWNARLLIEESSAVKCPSIAYHLTGSKKIQQELAKPGV 324
Query: 596 LEKFMGSGTSTSRVRDIF 649
LE+F+ + +++R F
Sbjct: 325 LERFLDNKEDIAKLRKCF 342
>At1g33770.1 68414.m04174 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 614
Score = 31.1 bits (67), Expect = 0.67
Identities = 16/54 (29%), Positives = 31/54 (57%)
Frame = +2
Query: 464 QYPTAREWDARLRVERSTAIKCPSIHYQLAGTKKVQQALAGPGVLEKFMGSGTS 625
+YP ++E DA++R E + K ++ ++ G + V++ L V +F+ SG S
Sbjct: 439 KYPPSKELDAKVRDEEAKRKKAEAVKWR--GHESVRRGLRDSKVTPEFIASGNS 490
>At1g68920.2 68414.m07888 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 485
Score = 30.3 bits (65), Expect = 1.2
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 556 NEEGPTSPGRSRGAGEVHGLRDEHLSRPGYIH 651
+E+ P SPG+ +G+ G + + GYIH
Sbjct: 270 DEQSPNSPGKKSNSGKQQGKQSSDPPKDGYIH 301
>At1g68920.1 68414.m07887 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 486
Score = 30.3 bits (65), Expect = 1.2
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 556 NEEGPTSPGRSRGAGEVHGLRDEHLSRPGYIH 651
+E+ P SPG+ +G+ G + + GYIH
Sbjct: 271 DEQSPNSPGKKSNSGKQQGKQSSDPPKDGYIH 302
>At5g67440.1 68418.m08503 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 579
Score = 29.5 bits (63), Expect = 2.0
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = -3
Query: 430 CYGMTVPVNDMSLVYVRSLFEYLNFSNSNE 341
CYGM V +N ++V VR EYL S E
Sbjct: 91 CYGMAVTLNAYNVVAVRCAAEYLEMYESIE 120
>At4g31820.1 68417.m04522 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 571
Score = 29.1 bits (62), Expect = 2.7
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -3
Query: 430 CYGMTVPVNDMSLVYVRSLFEYLNFS 353
CYGMTV +N ++ VR EYL +
Sbjct: 88 CYGMTVTLNAYNITAVRCAAEYLEMT 113
>At2g23050.1 68415.m02748 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 481
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = -3
Query: 439 VNDCYGMTVPVNDMSLVYVRSLFEYLNFSNSNE 341
V CYG+TV +N ++V VR E+L + + E
Sbjct: 90 VKFCYGITVTLNAYNVVAVRCAAEFLEMNETFE 122
>At2g40560.1 68415.m05004 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 303
Score = 27.9 bits (59), Expect = 6.2
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = -3
Query: 637 DARGARPGAHELLQHPGTGQGL 572
D R RP A ELL+HP QGL
Sbjct: 257 DMRSRRPTATELLKHPFITQGL 278
>At1g67900.2 68414.m07754 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 631
Score = 27.9 bits (59), Expect = 6.2
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -3
Query: 430 CYGMTVPVNDMSLVYVRSLFEYLNFSNSNE 341
CYG+T+ ++ ++V R EYL S E
Sbjct: 84 CYGITITISAYNIVAARCAAEYLQMSEEVE 113
>At1g67900.1 68414.m07753 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 631
Score = 27.9 bits (59), Expect = 6.2
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -3
Query: 430 CYGMTVPVNDMSLVYVRSLFEYLNFSNSNE 341
CYG+T+ ++ ++V R EYL S E
Sbjct: 84 CYGITITISAYNIVAARCAAEYLQMSEEVE 113
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,535,508
Number of Sequences: 28952
Number of extensions: 284720
Number of successful extensions: 796
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 779
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 796
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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