Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_K17
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g25950.1 68417.m03733 vacuolar ATP synthase, putative / V-ATP... 31 0.88
At1g79830.1 68414.m09326 expressed protein weak similarity to TA... 31 0.88
At4g40020.1 68417.m05666 hypothetical protein 29 2.0
At1g62440.1 68414.m07044 leucine-rich repeat family protein / ex... 29 2.7
At1g04220.1 68414.m00412 beta-ketoacyl-CoA synthase, putative St... 28 4.7
At5g52470.1 68418.m06510 fibrillarin 1 (FBR1) (FIB1) (SKIP7) ide... 28 6.2
At3g21215.1 68416.m02681 RNA-binding protein, putative contains ... 28 6.2
At5g43760.1 68418.m05352 beta-ketoacyl-CoA synthase, putative si... 27 8.2
At3g63070.1 68416.m07084 PWWP domain-containing protein putative... 27 8.2
At1g55420.1 68414.m06339 DC1 domain-containing protein contains ... 27 8.2
At1g35620.1 68414.m04425 thioredoxin family protein similar to S... 27 8.2
At1g05320.1 68414.m00539 myosin-related similar to non-muscle my... 27 8.2
At1g04600.1 68414.m00454 myosin, putative similar to myosin (GI:... 27 8.2
>At4g25950.1 68417.m03733 vacuolar ATP synthase, putative /
V-ATPase, putative / vacuolar proton pump, putative
similar to Swiss-Prot:O82629 vacuolar ATP synthase
subunit G 2 (V-ATPase G subunit 2, Vacuolar proton pump
G subunit 2) [Arabidopsis thaliana]
Length = 108
Score = 30.7 bits (66), Expect = 0.88
Identities = 19/70 (27%), Positives = 32/70 (45%)
Frame = +1
Query: 445 ATATAKLSEASQAADESERIRKALENRTNMEDDRVAILEAQLSQAKLIAEESDKKYEEVA 624
A TAKL+ QA DE+E+ + +R E Q + AK + +E+D + +
Sbjct: 26 AARTAKLARMKQAKDEAEKEMEEYRSRLEEEYQTQVSGTDQEADAKRLDDETDVRITNLK 85
Query: 625 RKLAMVXADL 654
+ V D+
Sbjct: 86 ESSSKVSKDI 95
>At1g79830.1 68414.m09326 expressed protein weak similarity to TATA
element modulatory factor (TMF) (Swiss-Prot:P82094)
[Homo sapiens]
Length = 927
Score = 30.7 bits (66), Expect = 0.88
Identities = 17/63 (26%), Positives = 31/63 (49%)
Frame = +1
Query: 448 TATAKLSEASQAADESERIRKALENRTNMEDDRVAILEAQLSQAKLIAEESDKKYEEVAR 627
T ++L EA A +E +++ R + R+ +LEAQLS + + K E+ +
Sbjct: 647 TLNSRLQEAESKAATAEERERSVNERLSQTLSRINVLEAQLSCLRAEQGQLSKSLEKERQ 706
Query: 628 KLA 636
+ A
Sbjct: 707 RAA 709
>At4g40020.1 68417.m05666 hypothetical protein
Length = 615
Score = 29.5 bits (63), Expect = 2.0
Identities = 20/58 (34%), Positives = 32/58 (55%), Gaps = 1/58 (1%)
Frame = +1
Query: 460 KLSEASQAADESERIRKAL-ENRTNMEDDRVAILEAQLSQAKLIAEESDKKYEEVARK 630
K++EA A D ++++K L E ME+++ L Q S K + E +KK EE +K
Sbjct: 348 KVNEAV-ANDNIKKLKKMLSEIEVAMEEEKQRSLNRQESMPKEVVEVVEKKIEEKEKK 404
>At1g62440.1 68414.m07044 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 826
Score = 29.1 bits (62), Expect = 2.7
Identities = 24/80 (30%), Positives = 32/80 (40%), Gaps = 3/80 (3%)
Frame = -3
Query: 640 PWPAYEQPLRISCPTPRR*ASPVTVEPPRWQRDHPPCWCGS---PAPCVFARIHRRPGWP 470
P P Y P+ S P P PVT PP + P S P+P + + + P P
Sbjct: 692 PSPVYYPPVTQSPPPPPVYYLPVTQSPPPPSPVYYPPVAKSPPPPSPVYYPPVTQSPPPP 751
Query: 469 RTAWRWRSRDAPRTSRGPPP 410
T + +P S PPP
Sbjct: 752 STPVEYHPPASPNQS--PPP 769
>At1g04220.1 68414.m00412 beta-ketoacyl-CoA synthase, putative
Strong similarity to beta-keto-Coa synthase gb|U37088
from Simmondsia chinensis, GI:4091810
Length = 528
Score = 28.3 bits (60), Expect = 4.7
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = -3
Query: 382 PLRTQLLLFA*FPLATVCGTPRTRPYIFPFPLSFDAF 272
P+ QLL FA V + +PYI F L+F+ F
Sbjct: 372 PMSEQLLFFATLVARKVFNVKKIKPYIPDFKLAFEHF 408
>At5g52470.1 68418.m06510 fibrillarin 1 (FBR1) (FIB1) (SKIP7)
identical to fibrillarin 1 GI:9965653 from [Arabidopsis
thaliana]; C-terminus identical to SKP1 interacting
partner 7 GI:10716959 from [Arabidopsis thaliana];
contains Pfam domain PF01269: Fibrillarin
Length = 308
Score = 27.9 bits (59), Expect = 6.2
Identities = 19/47 (40%), Positives = 25/47 (53%), Gaps = 4/47 (8%)
Frame = +3
Query: 411 GGGPREVRGAS--RDRHRQAVRGQ--PGRR*IRANTQGAGEPHQHGG 539
GGG RG S R R R A RG+ P R ++ ++ EPH+H G
Sbjct: 32 GGGRSGDRGRSGPRGRGRGAPRGRGGPPRGGMKGGSKVIVEPHRHAG 78
>At3g21215.1 68416.m02681 RNA-binding protein, putative contains RNA
recognition motif, Pfam:PF00076; contains AT-AC splice
sites at intron 8
Length = 339
Score = 27.9 bits (59), Expect = 6.2
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -3
Query: 640 PWPAYEQPLRISCPTPRR*ASPVTVEPPRWQRDHPPC 530
P+ Y P + PTP A+P + P + +D+PPC
Sbjct: 197 PYAGYHAP-PVPMPTPPPIAAPSSYVPVQNIKDNPPC 232
>At5g43760.1 68418.m05352 beta-ketoacyl-CoA synthase, putative
similar to beta-ketoacyl-CoA synthase [Simmondsia
chinensis][GI:1045614]
Length = 529
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = -3
Query: 382 PLRTQLLLFA*FPLATVCGTPRTRPYIFPFPLSFDAF 272
P+ QLL FA V + +PYI F L+F+ F
Sbjct: 380 PMSEQLLFFATLVARKVFKVKKIKPYIPDFKLAFEHF 416
>At3g63070.1 68416.m07084 PWWP domain-containing protein putative
transcription factor HUA2, Arabidopsis thaliana,
EMBL:AF116556
Length = 1347
Score = 27.5 bits (58), Expect = 8.2
Identities = 17/52 (32%), Positives = 23/52 (44%), Gaps = 1/52 (1%)
Frame = -3
Query: 562 PPRWQRDHPPCWCGSPAPCVFARIHRRPGWPR-TAWRWRSRDAPRTSRGPPP 410
PPR +PP GSP P +R+ PR ++ R P + PPP
Sbjct: 1167 PPRM---NPPMHYGSPEPSYSSRVSLSKSMPRGEGSNFQHRPYPSSHPPPPP 1215
>At1g55420.1 68414.m06339 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 725
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/32 (43%), Positives = 16/32 (50%), Gaps = 1/32 (3%)
Frame = -3
Query: 562 PPRWQRDHPPCWCGSPA-PCVFARIHRRPGWP 470
PP + R PPC PA P R RRP +P
Sbjct: 5 PPHFSRAKPPCRLSDPAQPHKLCR-RRRPSYP 35
>At1g35620.1 68414.m04425 thioredoxin family protein similar to
SP|Q43116 Protein disulfide isomerase precursor (PDI)
(EC 5.3.4.1) {Ricinus communis}; contains Pfam profile
PF00085: Thioredoxin
Length = 440
Score = 27.5 bits (58), Expect = 8.2
Identities = 15/37 (40%), Positives = 22/37 (59%)
Frame = +1
Query: 523 RTNMEDDRVAILEAQLSQAKLIAEESDKKYEEVARKL 633
R N E D A + A+L Q +IA+ + KY +ARK+
Sbjct: 66 RLNPELDAAAPILAKLKQPIVIAKLNADKYSRLARKI 102
>At1g05320.1 68414.m00539 myosin-related similar to non-muscle
myosin II heavy chain (GI:19879404) [Loligo pealei];
ESTs gb|AA042402,gb|ATTS1380 come from this gene
Length = 828
Score = 27.5 bits (58), Expect = 8.2
Identities = 17/65 (26%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
Frame = +1
Query: 469 EASQAADESERIRKALENRTNMEDDRVAILEAQLSQA---KLIAEESDKKYEEVARKLAM 639
EA + + ++ LE + + D+R + +A LSQA + E+ K EE++ K++
Sbjct: 112 EAEKLEELQKQSASELEEKLKISDERYSKTDALLSQALSQNSVLEQKLKSLEELSEKVSE 171
Query: 640 VXADL 654
+ + L
Sbjct: 172 LKSAL 176
>At1g04600.1 68414.m00454 myosin, putative similar to myosin
(GI:499047) [Arabidopsis thaliana]
Length = 1730
Score = 27.5 bits (58), Expect = 8.2
Identities = 25/99 (25%), Positives = 47/99 (47%), Gaps = 5/99 (5%)
Frame = +1
Query: 346 EIMRXEEAESE-----VAALNRRIQXXXXXXXXXXXXXATATAKLSEASQAADESERIRK 510
EI+ EAE+E V +L ++I + S A+ ++ER++K
Sbjct: 1095 EIVSNLEAENERLKALVGSLEKKINESGNNSTDEQEEGKYILKEESLTEDASIDNERVKK 1154
Query: 511 ALENRTNMEDDRVAILEAQLSQAKLIAEESDKKYEEVAR 627
+ ++ +D V+ LE ++ +E++KKYEE +R
Sbjct: 1155 LADENKDL-NDLVSSLEKKI-------DETEKKYEEASR 1185
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,113,455
Number of Sequences: 28952
Number of extensions: 246195
Number of successful extensions: 760
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 721
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 758
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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