Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_K08
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 281 3e-76
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 281 3e-76
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 281 3e-76
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 281 3e-76
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 153 7e-38
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 152 2e-37
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 83 2e-16
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 82 4e-16
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 45 4e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 45 4e-05
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 42 3e-04
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 40 0.001
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 40 0.001
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 40 0.001
At5g13650.2 68418.m01585 elongation factor family protein contai... 39 0.003
At5g13650.1 68418.m01584 elongation factor family protein contai... 39 0.003
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 39 0.003
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 38 0.004
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 38 0.006
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 36 0.031
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 36 0.031
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 35 0.054
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.22
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.2
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.5
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.5
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 30 1.5
At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 29 2.0
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.7
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 29 2.7
At4g20430.1 68417.m02981 subtilase family protein contains Pfam ... 27 8.2
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 281 bits (689), Expect = 3e-76
Identities = 132/170 (77%), Positives = 144/170 (84%)
Frame = +2
Query: 143 HLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKY 322
HLIYK GGIDKR I +FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KY
Sbjct: 26 HLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKY 85
Query: 323 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAFTLGV 502
Y T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FE GISK+GQTREHALLAFTLGV
Sbjct: 86 YCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGV 145
Query: 503 KQLIVXVNKMDSTEPPYSEPXFXEIXXEVSSYIKXIGYNPXAVAFVPISG 652
KQ+I NKMD+T P YS+ + EI EVSSY+K +GYNP + FVPISG
Sbjct: 146 KQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISG 195
Score = 54.4 bits (125), Expect = 6e-08
Identities = 25/27 (92%), Positives = 25/27 (92%)
Frame = +1
Query: 67 MGKEKTHINIVVIGHVDSGKSTTTGSL 147
MGKEK HINIVVIGHVDSGKSTTTG L
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHL 27
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 281 bits (689), Expect = 3e-76
Identities = 132/170 (77%), Positives = 144/170 (84%)
Frame = +2
Query: 143 HLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKY 322
HLIYK GGIDKR I +FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KY
Sbjct: 26 HLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKY 85
Query: 323 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAFTLGV 502
Y T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FE GISK+GQTREHALLAFTLGV
Sbjct: 86 YCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGV 145
Query: 503 KQLIVXVNKMDSTEPPYSEPXFXEIXXEVSSYIKXIGYNPXAVAFVPISG 652
KQ+I NKMD+T P YS+ + EI EVSSY+K +GYNP + FVPISG
Sbjct: 146 KQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISG 195
Score = 54.4 bits (125), Expect = 6e-08
Identities = 25/27 (92%), Positives = 25/27 (92%)
Frame = +1
Query: 67 MGKEKTHINIVVIGHVDSGKSTTTGSL 147
MGKEK HINIVVIGHVDSGKSTTTG L
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHL 27
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 281 bits (689), Expect = 3e-76
Identities = 132/170 (77%), Positives = 144/170 (84%)
Frame = +2
Query: 143 HLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKY 322
HLIYK GGIDKR I +FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KY
Sbjct: 26 HLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKY 85
Query: 323 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAFTLGV 502
Y T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FE GISK+GQTREHALLAFTLGV
Sbjct: 86 YCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGV 145
Query: 503 KQLIVXVNKMDSTEPPYSEPXFXEIXXEVSSYIKXIGYNPXAVAFVPISG 652
KQ+I NKMD+T P YS+ + EI EVSSY+K +GYNP + FVPISG
Sbjct: 146 KQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISG 195
Score = 54.4 bits (125), Expect = 6e-08
Identities = 25/27 (92%), Positives = 25/27 (92%)
Frame = +1
Query: 67 MGKEKTHINIVVIGHVDSGKSTTTGSL 147
MGKEK HINIVVIGHVDSGKSTTTG L
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHL 27
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 281 bits (689), Expect = 3e-76
Identities = 132/170 (77%), Positives = 144/170 (84%)
Frame = +2
Query: 143 HLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKY 322
HLIYK GGIDKR I +FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KY
Sbjct: 26 HLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKY 85
Query: 323 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAFTLGV 502
Y T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FE GISK+GQTREHALLAFTLGV
Sbjct: 86 YCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGV 145
Query: 503 KQLIVXVNKMDSTEPPYSEPXFXEIXXEVSSYIKXIGYNPXAVAFVPISG 652
KQ+I NKMD+T P YS+ + EI EVSSY+K +GYNP + FVPISG
Sbjct: 146 KQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISG 195
Score = 54.4 bits (125), Expect = 6e-08
Identities = 25/27 (92%), Positives = 25/27 (92%)
Frame = +1
Query: 67 MGKEKTHINIVVIGHVDSGKSTTTGSL 147
MGKEK HINIVVIGHVDSGKSTTTG L
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHL 27
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 153 bits (372), Expect = 7e-38
Identities = 75/170 (44%), Positives = 109/170 (64%), Gaps = 1/170 (0%)
Frame = +2
Query: 146 LIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKYY 325
+++ G +D R I K+EKEA + + S+ A+++D + ER +G T+++ FET
Sbjct: 121 ILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTNEEERLKGKTVEVGRAHFETESTR 180
Query: 326 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAFTLGVK 505
TI+DAPGH+ ++ NMI+G SQAD VL+++A GEFE G + GQTREH LA TLGV
Sbjct: 181 FTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYERGGQTREHVQLAKTLGVS 240
Query: 506 QLIVXVNKMDSTEPPYSEPXFXEIXXEVSSYIKXIGYNPXA-VAFVPISG 652
+LIV VNKMD +S+ + EI ++ ++K GYN V F+PISG
Sbjct: 241 KLIVVVNKMDDPTVNWSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISG 290
Score = 36.7 bits (81), Expect = 0.013
Identities = 14/24 (58%), Positives = 19/24 (79%)
Frame = +1
Query: 76 EKTHINIVVIGHVDSGKSTTTGSL 147
+K H+N+V IGHVD+GKST G +
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQI 121
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 152 bits (368), Expect = 2e-37
Identities = 72/169 (42%), Positives = 109/169 (64%), Gaps = 1/169 (0%)
Frame = +2
Query: 146 LIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKYY 325
L++ G I ++ + K+EKEA GKGSF YAW LD+ ERERGIT+ +A+ F + +++
Sbjct: 259 LLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAEERERGITMTVAVAYFNSKRHH 318
Query: 326 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISK-NGQTREHALLAFTLGV 502
V ++D+PGH+DF+ NMI G +QAD A+L++ A G FE G GQTREHA + GV
Sbjct: 319 VVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFDNLKGQTREHARVLRGFGV 378
Query: 503 KQLIVXVNKMDSTEPPYSEPXFXEIXXEVSSYIKXIGYNPXAVAFVPIS 649
+Q+IV +NKMD YS+ F I V S+++ + ++ ++P+S
Sbjct: 379 EQVIVAINKMDIV--GYSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLS 425
Score = 33.9 bits (74), Expect = 0.095
Identities = 12/22 (54%), Positives = 18/22 (81%)
Frame = +1
Query: 82 THINIVVIGHVDSGKSTTTGSL 147
+ +N+ ++GHVDSGKST +G L
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRL 259
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 83.0 bits (196), Expect = 2e-16
Identities = 42/102 (41%), Positives = 61/102 (59%)
Frame = +2
Query: 245 LDKLKAERERGITIDIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 424
+DK E++RGITI A ++ET K + +D PGH D++KNMITG +Q D +L+V+
Sbjct: 105 IDKAPEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGP 164
Query: 425 TGEFEXGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTEPP 550
G QT+EH LLA +GV L+ +NK+D + P
Sbjct: 165 DGPMP-------QTKEHILLARQVGVPSLVCFLNKVDVVDDP 199
Score = 33.9 bits (74), Expect = 0.095
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +1
Query: 73 KEKTHINIVVIGHVDSGKSTTTGSL 147
+ K H+N+ IGHVD GK+T T ++
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAI 87
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 81.8 bits (193), Expect = 4e-16
Identities = 41/100 (41%), Positives = 59/100 (59%)
Frame = +2
Query: 245 LDKLKAERERGITIDIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 424
+D ER RGITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 425 TGEFEXGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTE 544
G QT+EH LLA +GV ++V +NK D +
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNKEDQVD 209
Score = 37.5 bits (83), Expect = 0.008
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +1
Query: 64 KMGKEKTHINIVVIGHVDSGKSTTTGSLDLQM 159
K ++K H+NI IGHVD GK+T T +L + +
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMAL 103
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 45.2 bits (102), Expect = 4e-05
Identities = 26/77 (33%), Positives = 37/77 (48%)
Frame = +2
Query: 245 LDKLKAERERGITIDIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 424
+D + ERE+GITI A Y V IID PGH DF + D A+L++ +
Sbjct: 108 MDSMDLEREKGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSV 167
Query: 425 TGEFEXGISKNGQTREH 475
G I+ + Q R +
Sbjct: 168 GGVQSQSITVDRQMRRY 184
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 45.2 bits (102), Expect = 4e-05
Identities = 26/77 (33%), Positives = 37/77 (48%)
Frame = +2
Query: 245 LDKLKAERERGITIDIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 424
+D + ERE+GITI A Y V IID PGH DF + D A+L++ +
Sbjct: 108 MDSMDLEREKGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSV 167
Query: 425 TGEFEXGISKNGQTREH 475
G I+ + Q R +
Sbjct: 168 GGVQSQSITVDRQMRRY 184
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 42.3 bits (95), Expect = 3e-04
Identities = 23/66 (34%), Positives = 35/66 (53%), Gaps = 4/66 (6%)
Frame = +2
Query: 245 LDKLKAERERGITIDIAL----WKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 412
LD + ERERGITI + + +E + + +ID PGH DF + + + A+L+
Sbjct: 123 LDNMDLERERGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLV 182
Query: 413 VAAGTG 430
V A G
Sbjct: 183 VDASQG 188
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 40.3 bits (90), Expect = 0.001
Identities = 20/62 (32%), Positives = 33/62 (53%)
Frame = +2
Query: 245 LDKLKAERERGITIDIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 424
+D L E+ R IT+ + + Y + +ID+PGH DF + T +D A+++V A
Sbjct: 49 MDYLDEEQRRAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAV 108
Query: 425 TG 430
G
Sbjct: 109 EG 110
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 39.9 bits (89), Expect = 0.001
Identities = 22/75 (29%), Positives = 37/75 (49%)
Frame = +2
Query: 311 TXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAF 490
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 491 TLGVKQLIVXVNKMD 535
+ +K +I+ NK+D
Sbjct: 178 MMRLKHIIILQNKID 192
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 39.9 bits (89), Expect = 0.001
Identities = 22/75 (29%), Positives = 37/75 (49%)
Frame = +2
Query: 311 TXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAF 490
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 491 TLGVKQLIVXVNKMD 535
+ +K +I+ NK+D
Sbjct: 178 MMRLKHIIILQNKID 192
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 38.7 bits (86), Expect = 0.003
Identities = 36/120 (30%), Positives = 51/120 (42%), Gaps = 2/120 (1%)
Frame = +2
Query: 242 VLDKLKAERERGITIDIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAA 421
++D ERERGITI V IID PGH DF + + D +L+V +
Sbjct: 120 IMDSNDLERERGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDS 179
Query: 422 GTGEFEXGISKNGQTREHALLAFTLGVKQLIVXVNKMD--STEPPYSEPXFXEIXXEVSS 595
G QTR A G ++V VNK+D S P + E+ E+++
Sbjct: 180 VEGPMP-------QTRFVLKKALEFG-HAVVVVVNKIDRPSARPEFVVNSTFELFIELNA 231
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 38.7 bits (86), Expect = 0.003
Identities = 36/120 (30%), Positives = 51/120 (42%), Gaps = 2/120 (1%)
Frame = +2
Query: 242 VLDKLKAERERGITIDIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAA 421
++D ERERGITI V IID PGH DF + + D +L+V +
Sbjct: 119 IMDSNDLERERGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDS 178
Query: 422 GTGEFEXGISKNGQTREHALLAFTLGVKQLIVXVNKMD--STEPPYSEPXFXEIXXEVSS 595
G QTR A G ++V VNK+D S P + E+ E+++
Sbjct: 179 VEGPMP-------QTRFVLKKALEFG-HAVVVVVNKIDRPSARPEFVVNSTFELFIELNA 230
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 38.7 bits (86), Expect = 0.003
Identities = 22/71 (30%), Positives = 35/71 (49%)
Frame = +2
Query: 323 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAFTLGV 502
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 503 KQLIVXVNKMD 535
K +I+ NK+D
Sbjct: 174 KDIIIIQNKID 184
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 38.3 bits (85), Expect = 0.004
Identities = 21/71 (29%), Positives = 35/71 (49%)
Frame = +2
Query: 323 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAFTLGV 502
+V+ +D PGH + M+ G + D A+L++AA QT EH + +
Sbjct: 122 HVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSEHLAAVEIMQL 175
Query: 503 KQLIVXVNKMD 535
K +I+ NK+D
Sbjct: 176 KHIIILQNKID 186
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 37.9 bits (84), Expect = 0.006
Identities = 21/62 (33%), Positives = 31/62 (50%)
Frame = +2
Query: 245 LDKLKAERERGITIDIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 424
+D ++ E+ERGITI A K+ + IID PGH DF + D A+ + +
Sbjct: 136 MDWMEQEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSV 195
Query: 425 TG 430
G
Sbjct: 196 AG 197
Score = 27.9 bits (59), Expect = 6.2
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +1
Query: 79 KTHINIVVIGHVDSGKSTTT 138
K + NI ++ H+D+GK+TTT
Sbjct: 94 KDYRNIGIMAHIDAGKTTTT 113
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 35.5 bits (78), Expect = 0.031
Identities = 23/61 (37%), Positives = 29/61 (47%), Gaps = 5/61 (8%)
Frame = +2
Query: 263 ERERGITI-----DIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 427
E+ER I+I + L + Y I+D PGH +F M AD AVLIV A
Sbjct: 185 EQERNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAE 244
Query: 428 G 430
G
Sbjct: 245 G 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 35.5 bits (78), Expect = 0.031
Identities = 23/61 (37%), Positives = 29/61 (47%), Gaps = 5/61 (8%)
Frame = +2
Query: 263 ERERGITI-----DIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 427
E+ER I+I + L + Y I+D PGH +F M AD AVLIV A
Sbjct: 185 EQERNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAE 244
Query: 428 G 430
G
Sbjct: 245 G 245
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 34.7 bits (76), Expect = 0.054
Identities = 33/106 (31%), Positives = 46/106 (43%), Gaps = 9/106 (8%)
Frame = +2
Query: 245 LDKLKAERERGITIDIALWKF---------ETXKYYVTIIDAPGHRDFIKNMITGTSQAD 397
LDKL +RERGIT+ E Y + +ID PGH DF + S
Sbjct: 103 LDKL--QRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLSACQ 160
Query: 398 CAVLIVAAGTGEFEXGISKNGQTREHALLAFTLGVKQLIVXVNKMD 535
A+L+V A G QT + LAF + ++ +NK+D
Sbjct: 161 GALLVVDAAQG-------VQAQTVANFYLAFEANL-TIVPVINKID 198
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.22
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 326 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 430
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 30.3 bits (65), Expect = 1.2
Identities = 21/68 (30%), Positives = 34/68 (50%)
Frame = +2
Query: 332 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAFTLGVKQL 511
+ID PGH F G+S D A+L+V + + G+ QT E +L + +
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756
Query: 512 IVXVNKMD 535
I+ +NK+D
Sbjct: 757 IIALNKVD 764
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.5
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 332 IIDAPGHRDFIKNMITGTSQADCAVLIV 415
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.5
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 332 IIDAPGHRDFIKNMITGTSQADCAVLIV 415
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.9 bits (64), Expect = 1.5
Identities = 22/70 (31%), Positives = 33/70 (47%)
Frame = +2
Query: 326 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEXGISKNGQTREHALLAFTLGVK 505
+ +ID PGH F G++ D A+L+V + G+ QT E L VK
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610
Query: 506 QLIVXVNKMD 535
I+ +NK+D
Sbjct: 611 -FIIALNKVD 619
>At3g01360.1 68416.m00057 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 319
Score = 29.5 bits (63), Expect = 2.0
Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 1/71 (1%)
Frame = +2
Query: 335 IDAPGHRDFIKNMITGTSQADCAV-LIVAAGTGEFEXGISKNGQTREHALLAFTLGVKQL 511
I GH D+ + T Q +C + L+V TG F +KNG R+ LG +
Sbjct: 226 IKCKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELE 285
Query: 512 IVXVNKMDSTE 544
+ +DS E
Sbjct: 286 NLSTFTLDSDE 296
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.1 bits (62), Expect = 2.7
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +2
Query: 317 KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 415
+Y + +ID+PGH DF + D A+++V
Sbjct: 97 EYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +1
Query: 91 NIVVIGHVDSGKSTTTGSL 147
N+ VI HVD GKST T SL
Sbjct: 21 NMSVIAHVDHGKSTLTDSL 39
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 29.1 bits (62), Expect = 2.7
Identities = 27/99 (27%), Positives = 40/99 (40%), Gaps = 4/99 (4%)
Frame = +2
Query: 251 KLKAERERGITIDIALWKFETXK----YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 418
K+ A GIT I +K +D PGH F G D A+++VA
Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584
Query: 419 AGTGEFEXGISKNGQTREHALLAFTLGVKQLIVXVNKMD 535
A + GI QT E A+ +++ +NK+D
Sbjct: 585 A-----DDGI--RPQTNE-AIAHAKAAAVPIVIAINKID 615
>At4g20430.1 68417.m02981 subtilase family protein contains Pfam
profile: PF00082 subtilase family
Length = 856
Score = 27.5 bits (58), Expect = 8.2
Identities = 20/58 (34%), Positives = 27/58 (46%)
Frame = -3
Query: 508 LFDTEGESEQGMLTGLTVLRDTXFEFTGTGSYDEHSAISLRGSCDHVLDEISVSRSIN 335
+FDT E L G+ F +TGT ++ IS GS D L I+VS+ N
Sbjct: 714 IFDTSFEDYMSFLCGINGSAPVVFNYTGTNCLRNNATIS--GS-DLNLPSITVSKLNN 768
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,464,965
Number of Sequences: 28952
Number of extensions: 221936
Number of successful extensions: 589
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 545
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 578
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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