Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_J21
(605 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) riboso... 110 8e-25
At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B) 109 1e-24
At4g29950.2 68417.m04261 microtubule-associated protein identica... 30 1.0
At4g29950.1 68417.m04260 microtubule-associated protein identica... 30 1.0
At1g67280.1 68414.m07657 lactoylglutathione lyase, putative / gl... 30 1.4
At1g21740.1 68414.m02721 expressed protein contains Pfam domains... 28 4.2
At2g16390.1 68415.m01876 SNF2 domain-containing protein / helica... 28 5.5
At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP... 27 7.3
At4g31240.2 68417.m04435 expressed protein 27 9.6
At4g31240.1 68417.m04434 expressed protein 27 9.6
>At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) ribosomal
protein S6, Arabidopsis thaliana, PID:g2662469
Length = 250
Score = 110 bits (264), Expect = 8e-25
Identities = 64/121 (52%), Positives = 74/121 (61%), Gaps = 2/121 (1%)
Frame = +3
Query: 249 RAVLTNSRVRLLMSKGHSCYRP--RRDGXRKRKSVRGCIVDANLSVLALVIVRKGAXEIP 422
+ VLT RVRLL+ +G C+R RR G R+RKSVRGCIV +LSVL LVIV+KG ++P
Sbjct: 65 QGVLTPGRVRLLLHRGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKKGENDLP 124
Query: 423 GLTDGNVPRRLGPKRASKIRKLFNLSXXXXXXXXXXXXXLPAKEGKENAKPRHKAPXIQR 602
GLTD PR GPKRASKIRKLFNL K + K KAP IQR
Sbjct: 125 GLTDTEKPRMRGPKRASKIRKLFNLKKEDDVRTYVNTYR--RKFTNKKGKEVSKAPKIQR 182
Query: 603 L 605
L
Sbjct: 183 L 183
Score = 95.5 bits (227), Expect = 2e-20
Identities = 41/66 (62%), Positives = 50/66 (75%)
Frame = +2
Query: 56 MKLNVSYPATGCQKLFEVVDEHKLRIFYEKRMGAEVXADQLGDEWKGYVLRVAGGNDKQG 235
MK NV+ P TGCQK E+ D+ KLR FY+KR+ EV D LG+E+KGYV ++ GG DKQG
Sbjct: 1 MKFNVANPTTGCQKKLEIDDDQKLRAFYDKRISQEVSGDALGEEFKGYVFKIKGGCDKQG 60
Query: 236 FPMKQG 253
FPMKQG
Sbjct: 61 FPMKQG 66
>At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B)
Length = 249
Score = 109 bits (262), Expect = 1e-24
Identities = 64/121 (52%), Positives = 73/121 (60%), Gaps = 2/121 (1%)
Frame = +3
Query: 249 RAVLTNSRVRLLMSKGHSCYRP--RRDGXRKRKSVRGCIVDANLSVLALVIVRKGAXEIP 422
+ VLT RVRLL+ +G C+R RR G R+RKSVRGCIV +LSVL LVIV+KG ++P
Sbjct: 65 QGVLTPGRVRLLLHRGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKKGVSDLP 124
Query: 423 GLTDGNVPRRLGPKRASKIRKLFNLSXXXXXXXXXXXXXLPAKEGKENAKPRHKAPXIQR 602
GLTD PR GPKRASKIRKLFNL K K KAP IQR
Sbjct: 125 GLTDTEKPRMRGPKRASKIRKLFNLGKEDDVRKYVNTYRRTFTNKK--GKKVSKAPKIQR 182
Query: 603 L 605
L
Sbjct: 183 L 183
Score = 94.3 bits (224), Expect = 6e-20
Identities = 40/66 (60%), Positives = 50/66 (75%)
Frame = +2
Query: 56 MKLNVSYPATGCQKLFEVVDEHKLRIFYEKRMGAEVXADQLGDEWKGYVLRVAGGNDKQG 235
MK NV+ P TGCQK E+ D+ KLR F++KR+ EV D LG+E+KGYV ++ GG DKQG
Sbjct: 1 MKFNVANPTTGCQKKLEIDDDQKLRAFFDKRLSQEVSGDALGEEFKGYVFKIMGGCDKQG 60
Query: 236 FPMKQG 253
FPMKQG
Sbjct: 61 FPMKQG 66
>At4g29950.2 68417.m04261 microtubule-associated protein identical
to microtubule-associated protein GI:5032258 from
[Arabidopsis thaliana]; similar to TBC1 domain family
member 5 (Swiss-Prot:Q92609) [Homo sapiens]; contains
Pfam profile PF00566: TBC domain
Length = 703
Score = 30.3 bits (65), Expect = 1.0
Identities = 21/86 (24%), Positives = 36/86 (41%), Gaps = 1/86 (1%)
Frame = +2
Query: 80 ATGC-QKLFEVVDEHKLRIFYEKRMGAEVXADQLGDEWKGYVLRVAGGNDKQGFPMKQGR 256
A C Q+L ++ +R EK + A L D+ + L + G D+ P R
Sbjct: 276 AASCLQRLLNFPEKIDVRKIIEKAKSLQTLA--LDDDVRSSALSINDGFDQSISPAVPAR 333
Query: 257 PDKQPCSSSDVKGPLMLQTAPRWXEK 334
+ P S+ K PL++ W ++
Sbjct: 334 TNSFPSGSTSPKSPLIITPQSYWEDQ 359
>At4g29950.1 68417.m04260 microtubule-associated protein identical
to microtubule-associated protein GI:5032258 from
[Arabidopsis thaliana]; similar to TBC1 domain family
member 5 (Swiss-Prot:Q92609) [Homo sapiens]; contains
Pfam profile PF00566: TBC domain
Length = 828
Score = 30.3 bits (65), Expect = 1.0
Identities = 21/86 (24%), Positives = 36/86 (41%), Gaps = 1/86 (1%)
Frame = +2
Query: 80 ATGC-QKLFEVVDEHKLRIFYEKRMGAEVXADQLGDEWKGYVLRVAGGNDKQGFPMKQGR 256
A C Q+L ++ +R EK + A L D+ + L + G D+ P R
Sbjct: 401 AASCLQRLLNFPEKIDVRKIIEKAKSLQTLA--LDDDVRSSALSINDGFDQSISPAVPAR 458
Query: 257 PDKQPCSSSDVKGPLMLQTAPRWXEK 334
+ P S+ K PL++ W ++
Sbjct: 459 TNSFPSGSTSPKSPLIITPQSYWEDQ 484
>At1g67280.1 68414.m07657 lactoylglutathione lyase, putative /
glyoxalase I, putative similar to putative
lactoylglutathione lyase SP:Q39366, GI:2494843 from
[Brassica oleracea]
Length = 350
Score = 29.9 bits (64), Expect = 1.4
Identities = 20/70 (28%), Positives = 33/70 (47%)
Frame = +2
Query: 35 DGIDPQLMKLNVSYPATGCQKLFEVVDEHKLRIFYEKRMGAEVXADQLGDEWKGYVLRVA 214
DG +L++ + P CQ + V D + FYEK G E+ + E+K Y + +
Sbjct: 203 DGYKFELLERGPT-PEPLCQVMLRVGDLDRAIKFYEKAFGMELLRTRDNPEYK-YTIAMM 260
Query: 215 GGNDKQGFPM 244
G + FP+
Sbjct: 261 GYGPEDKFPV 270
>At1g21740.1 68414.m02721 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 953
Score = 28.3 bits (60), Expect = 4.2
Identities = 15/33 (45%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +2
Query: 92 QKLF-EVVDEHKLRIFYEKRMGAEVXADQLGDE 187
+KL+ EV DE KLR+ YE++ D LG E
Sbjct: 602 KKLYKEVKDEEKLRVVYEEKCRTLKKLDSLGAE 634
>At2g16390.1 68415.m01876 SNF2 domain-containing protein / helicase
domain-containing protein low similarity to RAD54
[Drosophila melanogaster] GI:1765914; contains Pfam
profiles PF00271: Helicase conserved C-terminal domain,
PF00176: SNF2 family N-terminal domain
Length = 888
Score = 27.9 bits (59), Expect = 5.5
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +3
Query: 414 EIPGLTDGNVPRRLGPKRASKIRKL 488
E+PGL D V L PK+ ++++KL
Sbjct: 620 ELPGLADFTVVLNLSPKQLNEVKKL 644
>At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP2)
identical to fatty acid multifunctional protein (AtMFP2)
GB:AF123254 [gi:4337027] (Arabidopsis thaliana) (fatty
acid beta-oxidation); contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 725
Score = 27.5 bits (58), Expect = 7.3
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +3
Query: 318 RDGXRKRKSVRGCIV--DANLSVLALVIVRKGAXEIPGLTD-GNVPRRL 455
R G K V +V D ++ + ++G ++PG+TD G VPR++
Sbjct: 264 RAGLEKEAEVASQVVKLDTTKGLIHVFFSQRGTAKVPGVTDRGLVPRKI 312
>At4g31240.2 68417.m04435 expressed protein
Length = 392
Score = 27.1 bits (57), Expect = 9.6
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = -2
Query: 250 LFHRESLLVVAASDTKYIALPFIA*LISLYFGAH 149
L H VVA + +K + + I LYFGAH
Sbjct: 180 LTHESRNYVVARNGSKVLVSKLVGKTIGLYFGAH 213
>At4g31240.1 68417.m04434 expressed protein
Length = 392
Score = 27.1 bits (57), Expect = 9.6
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = -2
Query: 250 LFHRESLLVVAASDTKYIALPFIA*LISLYFGAH 149
L H VVA + +K + + I LYFGAH
Sbjct: 180 LTHESRNYVVARNGSKVLVSKLVGKTIGLYFGAH 213
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,237,898
Number of Sequences: 28952
Number of extensions: 269448
Number of successful extensions: 671
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 655
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 669
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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