Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_J20
(655 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 215 4e-57
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 188 8e-49
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 93 4e-20
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 33 0.048
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 1.8
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 2.4
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 3.1
SPBC36.09 |sap61||U2 snRNP-associated protein sap61|Schizosaccha... 27 3.1
SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 4.1
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 26 4.1
SPAC1805.01c |ppk6|SPAPJ736.02c|serine/threonine protein kinase ... 26 4.1
SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 25 7.2
SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating tra... 25 7.2
SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2... 25 9.5
SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 25 9.5
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 25 9.5
SPAC27F1.08 |pdt1||Nramp family manganese ion transporter|Schizo... 25 9.5
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 215 bits (526), Expect = 4e-57
Identities = 107/147 (72%), Positives = 126/147 (85%)
Frame = +3
Query: 156 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 335
P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 336 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIXIVGDAXVRGTAXQP 515
YGLVV+VLI+G L++ + LY GFI LGAGL+VG +GLAAGFAI IVGDA VRGTA QP
Sbjct: 67 YGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQP 124
Query: 516 XLFVGMILILIFAEVLGLYGLIVAIYL 596
LFV MILILIFAEVLGLYGLIVA+ L
Sbjct: 125 RLFVAMILILIFAEVLGLYGLIVALLL 151
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 188 bits (457), Expect = 8e-49
Identities = 88/147 (59%), Positives = 112/147 (76%)
Frame = +3
Query: 156 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 335
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 336 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIXIVGDAXVRGTAXQP 515
YGLV++VLIAG + +Y L+ GFIHL AGLAVG +G+AAG+AI +VGD V+ Q
Sbjct: 67 YGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQD 126
Query: 516 XLFVGMILILIFAEVLGLYGLIVAIYL 596
+FV M+LILIFAEVLGLYGLIV + L
Sbjct: 127 RIFVSMVLILIFAEVLGLYGLIVGLIL 153
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 92.7 bits (220), Expect = 4e-20
Identities = 47/147 (31%), Positives = 79/147 (53%), Gaps = 6/147 (4%)
Frame = +3
Query: 174 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVA 353
+G++G AS + F +GAA+G GT I +V P + K++I ++ ++AIY L++A
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104
Query: 354 VLIAGALQE--PANY----PLYKGFIHLGAGLAVGFSGLAAGFAIXIVGDAXVRGTAXQP 515
++ + + + PA + Y GF G+ VG L G + I G + A
Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164
Query: 516 XLFVGMILILIFAEVLGLYGLIVAIYL 596
LFV ++++ IF VLGL+GLIV + +
Sbjct: 165 SLFVKVLVVEIFGSVLGLFGLIVGLLI 191
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 32.7 bits (71), Expect = 0.048
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +3
Query: 300 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIXIV 479
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246
Query: 480 GDAXVRGT 503
GD V T
Sbjct: 247 GDVCVSNT 254
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.5 bits (58), Expect = 1.8
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +3
Query: 159 IYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 338
+YGPF+ A+ FS Y +G G + S+ K +I A II Y
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137
Query: 339 GLVVAVLIAGAL 374
++AVL+ G L
Sbjct: 138 TTIIAVLLWGIL 149
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 27.1 bits (57), Expect = 2.4
Identities = 21/95 (22%), Positives = 47/95 (49%), Gaps = 6/95 (6%)
Frame = +3
Query: 135 NKMAENNP---IYGPFFG-VMGAASAIIFSALGAAYGTAKSGTGIAAMSVMR-PELIMKS 299
+K+A ++P I F+G ++G + F+ + + ++ G S LI+ S
Sbjct: 1424 DKIAMDSPRARITTMFYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMS 1483
Query: 300 IIPVVMAGIIAIYGLVVAVLIAGALQEPA-NYPLY 401
+ P+V++ IIA + + +++ L + + Y +Y
Sbjct: 1484 VAPLVLSAIIAFFFFCLGIMLRPILGDRSKTYGVY 1518
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 3.1
Identities = 18/57 (31%), Positives = 26/57 (45%)
Frame = -1
Query: 574 P*RPNTSAKIRIRIIPTNNXGX*AVPRTXASPTMXMAKPAARPENPTAKPAPKWMNP 404
P RP ++A ++ PT +VP ++P M P+A P A AP NP
Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPBC36.09 |sap61||U2 snRNP-associated protein
sap61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 492
Score = 26.6 bits (56), Expect = 3.1
Identities = 11/40 (27%), Positives = 22/40 (55%)
Frame = +2
Query: 521 IRRNDSYSYFR*SIGSLRTYRRHLPVHKINALNTHHSRRP 640
I +D + F S+G ++ + + P HK+ L+ +S +P
Sbjct: 76 INADDDLTEFYKSLGEIQEFHKKYPDHKVEDLSQLYSIKP 115
>SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 760
Score = 26.2 bits (55), Expect = 4.1
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -3
Query: 413 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 300
DEPF+K +L+ SN++S+ VD N+ D +D
Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85
>SPBC1826.01c |mot1||TATA-binding protein associated factor
Mot1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1953
Score = 26.2 bits (55), Expect = 4.1
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +1
Query: 4 YDPFLLSLAKLGPYLSCAKVVSWSECVQIVITVFGTCK 117
Y PFL A +GP AK+ S + +V+T + C+
Sbjct: 1440 YAPFLKVSAYVGPPAERAKIRSKMKKSDVVVTSYDICR 1477
>SPAC1805.01c |ppk6|SPAPJ736.02c|serine/threonine protein kinase
Ppk6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 4.1
Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = -2
Query: 108 PKNCDD--YLHTLTPRNYFCTRQIWPKLCKTQQEWVI 4
PK D YL T T Y+C I+P L K+ +++I
Sbjct: 288 PKTWDQRLYLTTKTTEGYYCPSMIYP-LSKSSFQYII 323
>SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 25.4 bits (53), Expect = 7.2
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -2
Query: 114 TGPKNCDDYLHTL 76
TGP N DDY+H +
Sbjct: 369 TGPSNTDDYIHRI 381
>SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating
transcription Rct1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 432
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/19 (57%), Positives = 13/19 (68%), Gaps = 1/19 (5%)
Frame = -2
Query: 114 TGPKNCDDYLHTLTP-RNY 61
TG KNCDD+L +P R Y
Sbjct: 402 TGNKNCDDHLRDKSPERRY 420
>SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase
Byr2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 659
Score = 25.0 bits (52), Expect = 9.5
Identities = 18/74 (24%), Positives = 33/74 (44%)
Frame = -3
Query: 596 QVDGDDKSVKTQYFSENKNKNHSDE*XRLXSSTTHACISHDAYGETGSQTRESYSQTSTQ 417
Q +G++KS K FSE+ + NH + ++ + I + S T E +
Sbjct: 329 QSNGNEKSSKYNVFSESAHGNH--QVLSFSPGSSPSFIEQPSPISPTSTTSEDTNTLEED 386
Query: 416 VDEPFVKGVVGWLL 375
D+ +K + G L+
Sbjct: 387 TDDQSIKWIRGALI 400
>SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit
Bgs2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1894
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +1
Query: 559 YWVFTDLSSPSTCTQNKRPEHTPLPSP 639
YW +T +SP+ +N R P P P
Sbjct: 497 YWYYTVFNSPTIIEKNFRQSVGPKPIP 523
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 25.0 bits (52), Expect = 9.5
Identities = 9/31 (29%), Positives = 16/31 (51%)
Frame = +1
Query: 34 LGPYLSCAKVVSWSECVQIVITVFGTCKYSH 126
LGPY+S W E + +++ G+ + H
Sbjct: 358 LGPYMSDPNCSCWYELIGVLVHAGGSTRSGH 388
>SPAC27F1.08 |pdt1||Nramp family manganese ion
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 521
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 4/41 (9%)
Frame = +3
Query: 390 YPLYKGFIHLGAG----LAVGFSGLAAGFAIXIVGDAXVRG 500
Y L K ++ + G +A+ FSG++AG+ I G G
Sbjct: 359 YDLLKEYVSISCGRLFAVALLFSGMSAGYVCTIAGQIVSEG 399
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,924,125
Number of Sequences: 5004
Number of extensions: 61192
Number of successful extensions: 194
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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