Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_J08
(651 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g30600.2 68415.m03729 BTB/POZ domain-containing protein conta... 31 0.88
At2g30600.1 68415.m03728 BTB/POZ domain-containing protein conta... 31 0.88
At2g04300.1 68415.m00422 leucine-rich repeat protein kinase, put... 29 2.7
At3g52460.1 68416.m05769 hydroxyproline-rich glycoprotein family... 29 3.5
At3g10100.1 68416.m01210 filament protein-related similar to YEA... 29 3.5
At3g07420.1 68416.m00884 asparaginyl-tRNA synthetase 2, cytoplas... 29 3.5
At4g14840.1 68417.m02281 expressed protein 28 4.7
At3g49590.2 68416.m05420 expressed protein 28 4.7
At3g49590.1 68416.m05419 expressed protein 28 4.7
At4g38770.1 68417.m05490 proline-rich family protein (PRP4) simi... 28 6.2
At4g20730.1 68417.m03013 filament protein-related similar to Cyt... 28 6.2
At2g13620.1 68415.m01501 cation/hydrogen exchanger, putative (CH... 28 6.2
At5g50400.1 68418.m06242 calcineurin-like phosphoesterase family... 27 8.2
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 27 8.2
>At2g30600.2 68415.m03729 BTB/POZ domain-containing protein contains
Pfam PF00651: BTB/POZ domain; contains Interpro
IPR000210/ PS50097: BTBB/POZ domain; similar to MigA
(GI:1841872) [Dictyostelium discoideum]
Length = 809
Score = 30.7 bits (66), Expect = 0.88
Identities = 26/93 (27%), Positives = 45/93 (48%), Gaps = 6/93 (6%)
Frame = +1
Query: 217 QESDPMYEVMIGGWGNAKSVIRKNXTKPDKVEIESPGILNGG-EYRGFWVRWDSGIISAG 393
+++ P Y V+IG N + I+ + V+ E+ + E++ +W+ G+IS G
Sbjct: 60 KDNSPHYIVIIGSNRNRRLKIQVDGKSV--VDEEASDLCRCSLEFQSYWISIYDGLISIG 117
Query: 394 REGEAIPF----ISWSDPEP-FPVYYVGVCTGW 477
+ PF W DP+P V YVG+ + W
Sbjct: 118 K--GRYPFQNLVFKWQDPKPNCNVQYVGL-SSW 147
>At2g30600.1 68415.m03728 BTB/POZ domain-containing protein contains
Pfam PF00651: BTB/POZ domain; contains Interpro
IPR000210/ PS50097: BTBB/POZ domain; similar to MigA
(GI:1841872) [Dictyostelium discoideum]
Length = 809
Score = 30.7 bits (66), Expect = 0.88
Identities = 26/93 (27%), Positives = 45/93 (48%), Gaps = 6/93 (6%)
Frame = +1
Query: 217 QESDPMYEVMIGGWGNAKSVIRKNXTKPDKVEIESPGILNGG-EYRGFWVRWDSGIISAG 393
+++ P Y V+IG N + I+ + V+ E+ + E++ +W+ G+IS G
Sbjct: 60 KDNSPHYIVIIGSNRNRRLKIQVDGKSV--VDEEASDLCRCSLEFQSYWISIYDGLISIG 117
Query: 394 REGEAIPF----ISWSDPEP-FPVYYVGVCTGW 477
+ PF W DP+P V YVG+ + W
Sbjct: 118 K--GRYPFQNLVFKWQDPKPNCNVQYVGL-SSW 147
>At2g04300.1 68415.m00422 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 851
Score = 29.1 bits (62), Expect = 2.7
Identities = 15/56 (26%), Positives = 27/56 (48%)
Frame = +1
Query: 226 DPMYEVMIGGWGNAKSVIRKNXTKPDKVEIESPGILNGGEYRGFWVRWDSGIISAG 393
D ++ + +G ++S + T+ D V +PG L+ YR W+ S + S G
Sbjct: 675 DEHFQAKLADFGLSRSFPLEGETRVDTVVAGTPGYLDPEYYRTNWLNEKSDVYSFG 730
>At3g52460.1 68416.m05769 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 300
Score = 28.7 bits (61), Expect = 3.5
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = -2
Query: 446 GKGSGSDQDMNGIASPSRPAEIMPLSQRTQKPRYSPPLRIPG 321
G G S++D+N P P + P +TQ+ Y P + PG
Sbjct: 16 GPGQNSERDIN--QPPPPPPQSQPPPPQTQQQTYPPVMGYPG 55
>At3g10100.1 68416.m01210 filament protein-related similar to YEAST
NUF1 protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; similar to
Myosin heavy chain, smooth muscle isoform (SMMHC)
(SP:P35749) {Homo sapiens}
Length = 1004
Score = 28.7 bits (61), Expect = 3.5
Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +2
Query: 440 LSQFTTSESAQAGVPQAPGKSKMERNSILRTXXSISLDLSPLVLWNS-ITADRT 598
LSQF + P ++ E N+ L + DL P+V WN+ ITA +T
Sbjct: 70 LSQFDPLIDLEDAYNTQPQYTEAEVNARLLHEDYVEFDLDPVVSWNTEITAKKT 123
>At3g07420.1 68416.m00884 asparaginyl-tRNA synthetase 2, cytoplasmic
/ asparagine-tRNA ligase 2 (SYNC2) nearly identical to
SP|Q9SW95; HMM hit: tRNA synthetases class II
Length = 638
Score = 28.7 bits (61), Expect = 3.5
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = +1
Query: 241 VMIGGWGNAKSVIRKNXTKPDKVEIESPGILNGGE 345
V+IGGW + ++KN P + +P +GG+
Sbjct: 50 VVIGGWVKSARAVKKNSPPPPLPVVAAPSPSSGGD 84
>At4g14840.1 68417.m02281 expressed protein
Length = 555
Score = 28.3 bits (60), Expect = 4.7
Identities = 18/52 (34%), Positives = 25/52 (48%), Gaps = 2/52 (3%)
Frame = +2
Query: 344 NIVVFGFVGIAALS--PLDARVKLFHSYLGLIPNLSQFTTSESAQAGVPQAP 493
+I V G+ A+S P+D + F SYL + NL Q SE+ Q P
Sbjct: 294 HIYVSSMNGVIAVSHPPVDINPEEFDSYLNSLENLLQQQPSEAGQESSSSLP 345
>At3g49590.2 68416.m05420 expressed protein
Length = 603
Score = 28.3 bits (60), Expect = 4.7
Identities = 19/60 (31%), Positives = 32/60 (53%), Gaps = 3/60 (5%)
Frame = -2
Query: 377 PLSQRTQKPRYSPPLRIPG---LSISTLSGLVXFFLMTLLAFPQPPIITSYIGSDSCGPV 207
P+++ ++ R++P PG S++ S L F L + P P IIT Y+GS + P+
Sbjct: 196 PVTETMKEFRFAPVEVPPGRLCASVTYRSDLSDFNLGAHITLP-PRIITDYVGSPATDPM 254
>At3g49590.1 68416.m05419 expressed protein
Length = 603
Score = 28.3 bits (60), Expect = 4.7
Identities = 19/60 (31%), Positives = 32/60 (53%), Gaps = 3/60 (5%)
Frame = -2
Query: 377 PLSQRTQKPRYSPPLRIPG---LSISTLSGLVXFFLMTLLAFPQPPIITSYIGSDSCGPV 207
P+++ ++ R++P PG S++ S L F L + P P IIT Y+GS + P+
Sbjct: 196 PVTETMKEFRFAPVEVPPGRLCASVTYRSDLSDFNLGAHITLP-PRIITDYVGSPATDPM 254
>At4g38770.1 68417.m05490 proline-rich family protein (PRP4) similar
to proline-rich protein [Arabidopsis thaliana]
gi|6782442|gb|AAF28388; contains proline-rich extensin
domains, INTERPRO:IPR002965
Length = 448
Score = 27.9 bits (59), Expect = 6.2
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = -2
Query: 392 PAEIMPLSQRTQKPRYSPPLRIP 324
P E+ P ++ P+YSPP+ +P
Sbjct: 171 PLELPPFLKKPCPPKYSPPVEVP 193
>At4g20730.1 68417.m03013 filament protein-related similar to
Cytadherence high molecular weight protein 2 (SP:P47460)
[Mycoplasma genitalium]; similar to YEAST NUF1
protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; also
SP|Q9UKX2|MYH2_HUMAN Myosin heavy chain, skeletal
muscle, SP|P31732|OV71_ONCVO Muscle cell intermediate
filament protein SP|P12882|MYH1_HUMAN Myosin heavy
chain, skeletal muscle,. SP|Q17107|AV71_ACAVI Muscle
cell intermediate filament protein
Length = 800
Score = 27.9 bits (59), Expect = 6.2
Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +2
Query: 440 LSQFTTSESAQAGVPQAPGKSKMERNSILRTXXSISLDLSPLVLWNS-ITADRT 598
L+QF + P ++ E N+ L + DL P+V WN+ ITA +T
Sbjct: 71 LAQFDPLIDLEDAYNTQPQYTEAEVNARLLHEDHVEFDLDPVVSWNTEITAKKT 124
>At2g13620.1 68415.m01501 cation/hydrogen exchanger, putative
(CHX15) monovalent cation:proton antiporter family 2
(CPA2) member, PMID:11500563
Length = 821
Score = 27.9 bits (59), Expect = 6.2
Identities = 17/56 (30%), Positives = 27/56 (48%)
Frame = -2
Query: 347 YSPPLRIPGLSISTLSGLVXFFLMTLLAFPQPPIITSYIGSDSCGPVVSAMWASFA 180
+S PL + L ++ + + FF+ L F QP +I+ +G GP V FA
Sbjct: 35 FSLPLFV--LQLTLVVVVTRFFVFILKPFRQPRVISEILGGIVLGPSVLGRSTKFA 88
>At5g50400.1 68418.m06242 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 611
Score = 27.5 bits (58), Expect = 8.2
Identities = 21/73 (28%), Positives = 30/73 (41%), Gaps = 2/73 (2%)
Frame = +1
Query: 217 QESDPMYEVMIGGWGNAKSVIRKNXTK--PDKVEIESPGILNGGEYRGFWVRWDSGIISA 390
Q +D + + GG N V N K + P + G ++ V W SG
Sbjct: 135 QRADFSFALFTGGLSNPTLVSVSNHVSFINPKAPVY-PRLALGKKWDEMTVTWTSGY--- 190
Query: 391 GREGEAIPFISWS 429
GEA+PF+ WS
Sbjct: 191 -NIGEAVPFVEWS 202
>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to acyl
CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
Length = 665
Score = 27.5 bits (58), Expect = 8.2
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -1
Query: 420 YEWNSFTLASSGDNAAIPTNPKTTIFPSVKNSG 322
+ WN F+L + D A +P KT I + SG
Sbjct: 201 FSWNEFSLMGNLDEANLPRKRKTDICTIMYTSG 233
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,256,430
Number of Sequences: 28952
Number of extensions: 313131
Number of successful extensions: 897
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 858
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 897
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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